Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	F	Q	L	S	V	Q	D	I	H	P	G	E	K	A	G	D	K	E	E	A	I	R	Q	V	A	A	A	L	V	Q	A	G	N	V	A	E	G	Y	V	N	G	M	L	A	R	E	Q	Q	T	S	T	F	L	G	N	G	I	A	I
Secondary structure
SS confidence
Disorder		?	?	?	?	?																																														?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		P	H	G	T	T	D	T	R	D	Q	V	L	K	T	G	V	Q	V	F	Q	F	P	E	G	V	T	W	G	D	G	Q	V	A	Y	V	A	I	G	I	A	A	S	S	D	E	H	L	G	L	L	R	Q	L	T	H	V	L	S	D	D
Secondary structure
SS confidence
Disorder																											?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		S	V	A	E	Q	L	K	S	A	T	T	A	E	E	L	R	A	L	L	M	G	E	K	Q	S	E	Q	L	K	L	D	N	E	M	L	T	L	D	I	V	A	S	D	L	L	T	L	Q	A	L	N	A	A	R	L	K	E	A	G	A
Secondary structure
SS confidence
Disorder																								?	?	?	?	?	?	?	?	?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		V	D	A	T	F	V	T	K	A	I	N	E	Q	P	L	N	L	G	Q	G	I	W	L	S	D	S	A	E	G	N	L	R	S	A	I	A	V	S	R	A	A	N	A	F	D	V	D	G	E	T	A	A	M	L	V	S	V	A	M	N
Secondary structure
SS confidence
Disorder		?											?	?		?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		D	D	Q	P	I	A	V	L	K	R	L	A	D	L	L	L	D	N	K	A	D	R	L	L	K	A	D	A	A	T	L	L	A	L	L	T	S	D	D	A	P	T	D	D	V	L	S	A	E	F	V	V	R	N	E	H	G	L	H	A
Secondary structure
SS confidence
Disorder																						?					?		?							?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		R	P	G	T	M	L	V	N	T	I	K	Q	F	N	S	D	I	T	V	T	N	L	D	G	T	G	K	P	A	N	G	R	S	L	M	K	V	V	A	L	G	V	K	K	G	H	R	L	R	F	T	A	Q	G	A	D	A	E	Q	A
Secondary structure
SS confidence
Disorder																									?	?	?	?	?	?	?	?									?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.
Sequence		L	K	A	I	G	D	A	I	A	A	G	L	G	E	G	A
Secondary structure
SS confidence
Disorder											?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1a3a chain A Region: 1 - 143 Aligned: 143 Modelled: 143 Confidence: 100.0% Identity: 47% Fold: Phoshotransferase/anion transport protein Superfamily: Phoshotransferase/anion transport protein Family: IIA domain of mannitol-specific and ntr phosphotransferase EII Phyre2 PDB 3bjv chain A Region: 4 - 149 Aligned: 144 Modelled: 144 Confidence: 100.0% Identity: 27% PDB header:transferase Chain: A: PDB Molecule:rmpa; PDBTitle: the crystal structure of a putative pts iia(ptxa) from streptococcus2 mutans Phyre2 PDB 2oqt chain D Region: 4 - 146 Aligned: 141 Modelled: 143 Confidence: 100.0% Identity: 22% PDB header:transferase Chain: D: PDB Molecule:hypothetical protein spy0176; PDBTitle: structural genomics, the crystal structure of a putative2 pts iia domain from streptococcus pyogenes m1 gas Phyre2 PDB 3oxp chain B Region: 4 - 141 Aligned: 136 Modelled: 137 Confidence: 100.0% Identity: 25% PDB header:transferase Chain: B: PDB Molecule:phosphotransferase enzyme ii, a component; PDBTitle: structure of phosphotransferase enzyme ii, a component from yersinia2 pestis co92 at 1.2 a resolution Phyre2 PDB 3oxp chain A Region: 4 - 141 Aligned: 136 Modelled: 138 Confidence: 100.0% Identity: 25% PDB header:transferase Chain: A: PDB Molecule:phosphotransferase enzyme ii, a component; PDBTitle: structure of phosphotransferase enzyme ii, a component from yersinia2 pestis co92 at 1.2 a resolution Phyre2 PDB 2oq3 chain A Region: 4 - 141 Aligned: 136 Modelled: 138 Confidence: 100.0% Identity: 25% PDB header:transferase Chain: A: PDB Molecule:mannitol-specific cryptic phosphotransferase PDBTitle: solution structure of the mannitol- specific cryptic2 phosphotransferase enzyme iia cmtb from escherichia coli Phyre2 PDB 3urr chain B Region: 4 - 144 Aligned: 137 Modelled: 141 Confidence: 100.0% Identity: 25% PDB header:transferase Chain: B: PDB Molecule:pts iia-like nitrogen-regulatory protein ptsn; PDBTitle: structure of pts iia-like nitrogen-regulatory protein ptsn (bth_i0484)2 (ptsn) Phyre2 PDB 2a0j chain A Region: 4 - 139 Aligned: 133 Modelled: 136 Confidence: 100.0% Identity: 29% PDB header:transferase Chain: A: PDB Molecule:pts system, nitrogen regulatory iia protein; PDBTitle: crystal structure of nitrogen regulatory protein iia-ntr from2 neisseria meningitidis Phyre2 PDB 1a6j chain A Region: 4 - 143 Aligned: 138 Modelled: 140 Confidence: 100.0% Identity: 23% Fold: Phoshotransferase/anion transport protein Superfamily: Phoshotransferase/anion transport protein Family: IIA domain of mannitol-specific and ntr phosphotransferase EII Phyre2 PDB 2hpr chain A Region: 287 - 374 Aligned: 85 Modelled: 88 Confidence: 99.9% Identity: 40% Fold: HPr-like Superfamily: HPr-like Family: HPr-like Phyre2 PDB 1pch chain A Region: 287 - 375 Aligned: 86 Modelled: 89 Confidence: 99.9% Identity: 35% Fold: HPr-like Superfamily: HPr-like Family: HPr-like Phyre2 PDB 1ka5 chain A Region: 286 - 374 Aligned: 86 Modelled: 89 Confidence: 99.9% Identity: 41% Fold: HPr-like Superfamily: HPr-like Family: HPr-like Phyre2 PDB 1qr5 chain A Region: 286 - 374 Aligned: 86 Modelled: 89 Confidence: 99.9% Identity: 41% Fold: HPr-like Superfamily: HPr-like Family: HPr-like Phyre2 PDB 1xiz chain A Region: 1 - 141 Aligned: 138 Modelled: 141 Confidence: 99.9% Identity: 23% Fold: Phoshotransferase/anion transport protein Superfamily: Phoshotransferase/anion transport protein Family: IIA domain of mannitol-specific and ntr phosphotransferase EII Phyre2 PDB 3ihs chain B Region: 283 - 369 Aligned: 84 Modelled: 87 Confidence: 99.9% Identity: 29% PDB header:transport protein Chain: B: PDB Molecule:phosphocarrier protein hpr; PDBTitle: crystal structure of a phosphocarrier protein hpr from2 bacillus anthracis str. ames Phyre2 PDB 1mo1 chain A Region: 286 - 374 Aligned: 86 Modelled: 89 Confidence: 99.9% Identity: 28% Fold: HPr-like Superfamily: HPr-like Family: HPr-like Phyre2 PDB 3le1 chain B Region: 286 - 372 Aligned: 84 Modelled: 87 Confidence: 99.9% Identity: 36% PDB header:transferase Chain: B: PDB Molecule:phosphotransferase system, hpr-related proteins; PDBTitle: crystal structure of apohpr monomer from thermoanaerobacter2 tengcongensis Phyre2 PDB 2nzu chain L domain 1 Region: 287 - 374 Aligned: 84 Modelled: 88 Confidence: 99.9% Identity: 40% Fold: HPr-like Superfamily: HPr-like Family: HPr-like Phyre2 PDB 1cm3 chain A Region: 286 - 370 Aligned: 81 Modelled: 85 Confidence: 99.9% Identity: 33% Fold: HPr-like Superfamily: HPr-like Family: HPr-like Phyre2 PDB 1ptf chain A Region: 286 - 371 Aligned: 83 Modelled: 86 Confidence: 99.9% Identity: 40% Fold: HPr-like Superfamily: HPr-like Family: HPr-like Phyre2 1 d1a3aa_ 2 c3bjvA_ 3 c2oqtD_ 4 c3oxpB_ 5 c3oxpA_ 6 c2oq3A_ 7 c3urrB_ 8 c2a0jA_ 9 d1a6ja_ 10 d2hpra_ 11 d1pcha_ 12 d1ka5a_ 13 d1qr5a_ 14 d1xiza_ 15 c3ihsB_ 16 d1mo1a_ 17 c3le1B_ 18 d2nzul1 19 d1cm3a_ 20 d1ptfa_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1a3aa_ 100.0 47 Fold:Phoshotransferase/anion transport protein Superfamily:Phoshotransferase/anion transport protein Family:IIA domain of mannitol-specific and ntr phosphotransferase EII 2 c3bjvA_ 100.0 27 PDB header:transferase Chain: A: PDB Molecule:rmpa; PDBTitle: the crystal structure of a putative pts iia(ptxa) from streptococcus2 mutans 3 c2oqtD_ 100.0 22 PDB header:transferase Chain: D: PDB Molecule:hypothetical protein spy0176; PDBTitle: structural genomics, the crystal structure of a putative2 pts iia domain from streptococcus pyogenes m1 gas 4 c3oxpB_ 100.0 25 PDB header:transferase Chain: B: PDB Molecule:phosphotransferase enzyme ii, a component; PDBTitle: structure of phosphotransferase enzyme ii, a component from yersinia2 pestis co92 at 1.2 a resolution 5 c3oxpA_ 100.0 25 PDB header:transferase Chain: A: PDB Molecule:phosphotransferase enzyme ii, a component; PDBTitle: structure of phosphotransferase enzyme ii, a component from yersinia2 pestis co92 at 1.2 a resolution 6 c2oq3A_ 100.0 25 PDB header:transferase Chain: A: PDB Molecule:mannitol-specific cryptic phosphotransferase PDBTitle: solution structure of the mannitol- specific cryptic2 phosphotransferase enzyme iia cmtb from escherichia coli 7 c3urrB_ 100.0 25 PDB header:transferase Chain: B: PDB Molecule:pts iia-like nitrogen-regulatory protein ptsn; PDBTitle: structure of pts iia-like nitrogen-regulatory protein ptsn (bth_i0484)2 (ptsn) 8 c2a0jA_ 100.0 29 PDB header:transferase Chain: A: PDB Molecule:pts system, nitrogen regulatory iia protein; PDBTitle: crystal structure of nitrogen regulatory protein iia-ntr from2 neisseria meningitidis 9 d1a6ja_ 100.0 23 Fold:Phoshotransferase/anion transport protein Superfamily:Phoshotransferase/anion transport protein Family:IIA domain of mannitol-specific and ntr phosphotransferase EII 10 d2hpra_ 99.9 40 Fold:HPr-like Superfamily:HPr-like Family:HPr-like 11 d1pcha_ 99.9 35 Fold:HPr-like Superfamily:HPr-like Family:HPr-like 12 d1ka5a_ 99.9 41 Fold:HPr-like Superfamily:HPr-like Family:HPr-like 13 d1qr5a_ 99.9 41 Fold:HPr-like Superfamily:HPr-like Family:HPr-like 14 d1xiza_ 99.9 23 Fold:Phoshotransferase/anion transport protein Superfamily:Phoshotransferase/anion transport protein Family:IIA domain of mannitol-specific and ntr phosphotransferase EII 15 c3ihsB_ 99.9 29 PDB header:transport protein Chain: B: PDB Molecule:phosphocarrier protein hpr; PDBTitle: crystal structure of a phosphocarrier protein hpr from2 bacillus anthracis str. ames 16 d1mo1a_ 99.9 28 Fold:HPr-like Superfamily:HPr-like Family:HPr-like 17 c3le1B_ 99.9 36 PDB header:transferase Chain: B: PDB Molecule:phosphotransferase system, hpr-related proteins; PDBTitle: crystal structure of apohpr monomer from thermoanaerobacter2 tengcongensis 18 d2nzul1 99.9 40 Fold:HPr-like Superfamily:HPr-like Family:HPr-like 19 d1cm3a_ 99.9 33 Fold:HPr-like Superfamily:HPr-like Family:HPr-like 20 d1ptfa_ 99.9 40 Fold:HPr-like Superfamily:HPr-like Family:HPr-like 21 d1zvvj1 not modelled 99.9 30 Fold:HPr-like Superfamily:HPr-like Family:HPr-like 22 c1hynQ_ not modelled 95.5 13 PDB header:membrane protein Chain: Q: PDB Molecule:band 3 anion transport protein; PDBTitle: crystal structure of the cytoplasmic domain of human2 erythrocyte band-3 protein 23 d1hynp_ not modelled 95.5 14 Fold:Phoshotransferase/anion transport protein Superfamily:Phoshotransferase/anion transport protein Family:Anion transport protein, cytoplasmic domain 24 c2jpiA_ not modelled 43.7 14 PDB header:structural genomics Chain: A: PDB Molecule:hypothetical protein; PDBTitle: chemical shift assignments of pa4090 from pseudomonas2 aeruginosa 25 c2dgbA_ not modelled 29.7 19 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical protein purs; PDBTitle: structure of thermus thermophilus purs in the p21 form 26 d2vo1a1 not modelled 28.9 14 Fold:P-loop containing nucleoside triphosphate hydrolases Superfamily:P-loop containing nucleoside triphosphate hydrolases Family:Nitrogenase iron protein-like 27 c3bmbB_ not modelled 26.9 21 PDB header:rna binding protein Chain: B: PDB Molecule:regulator of nucleoside diphosphate kinase; PDBTitle: crystal structure of a new rna polymerase interacting2 protein 28 d1gtda_ not modelled 24.9 16 Fold:PurS-like Superfamily:PurS-like Family:PurS subunit of FGAM synthetase 29 d2ihta1 not modelled 24.2 31 Fold:DHS-like NAD/FAD-binding domain Superfamily:DHS-like NAD/FAD-binding domain Family:Pyruvate oxidase and decarboxylase, middle domain 30 c2p73A_ not modelled 20.8 25 PDB header:transferase Chain: A: PDB Molecule:putative glycosyltransferase (mannosyltransferase) involved PDBTitle: crystal structure of a glycosyltransferase involved in the2 glycosylation of the major capsid of pbcv-1 31 d5csma_ not modelled 19.7 16 Fold:Chorismate mutase II Superfamily:Chorismate mutase II Family:Allosteric chorismate mutase 32 d2mhra_ not modelled 18.3 24 Fold:Four-helical up-and-down bundle Superfamily:Hemerythrin-like Family:Hemerythrin-like 33 c3citB_ not modelled 17.6 38 PDB header:transferase Chain: B: PDB Molecule:sensor histidine kinase; PDBTitle: crystal structure of the gaf domain of a putative sensor histidine2 kinase from pseudomonas syringae pv. tomato 34 d1vq3a_ not modelled 15.4 16 Fold:PurS-like Superfamily:PurS-like Family:PurS subunit of FGAM synthetase 35 d1zpda1 not modelled 13.5 12 Fold:DHS-like NAD/FAD-binding domain Superfamily:DHS-like NAD/FAD-binding domain Family:Pyruvate oxidase and decarboxylase, middle domain 36 d1r11a3 not modelled 13.1 18 Fold:MutS N-terminal domain-like Superfamily:tRNA-intron endonuclease N-terminal domain-like Family:tRNA-intron endonuclease N-terminal domain-like 37 d1xi3a_ not modelled 12.1 14 Fold:TIM beta/alpha-barrel Superfamily:Thiamin phosphate synthase Family:Thiamin phosphate synthase 38 d1umdb2 not modelled 11.8 22 Fold:TK C-terminal domain-like Superfamily:TK C-terminal domain-like Family:Branched-chain alpha-keto acid dehydrogenase beta-subunit, C-terminal-domain 39 c2zw2B_ not modelled 11.0 15 PDB header:ligase Chain: B: PDB Molecule:putative uncharacterized protein sts178; PDBTitle: crystal structure of formylglycinamide ribonucleotide amidotransferase2 iii from sulfolobus tokodaii (stpurs) 40 c2d5wA_ not modelled 10.6 17 PDB header:peptide binding protein Chain: A: PDB Molecule:peptide abc transporter, peptide-binding protein; PDBTitle: the crystal structure of oligopeptide binding protein from thermus2 thermophilus hb8 complexed with pentapeptide 41 d2ifea_ not modelled 10.1 18 Fold:IF3-like Superfamily:Translation initiation factor IF3, C-terminal domain Family:Translation initiation factor IF3, C-terminal domain 42 d1pvda1 not modelled 8.6 12 Fold:DHS-like NAD/FAD-binding domain Superfamily:DHS-like NAD/FAD-binding domain Family:Pyruvate oxidase and decarboxylase, middle domain 43 c3m8uA_ not modelled 8.4 16 PDB header:transport protein Chain: A: PDB Molecule:heme-binding protein a; PDBTitle: crystal structure of glutathione-binding protein a (gbpa) from2 haemophilus parasuis sh0165 in complex with glutathione disulfide3 (gssg) 44 d1hrba_ not modelled 8.4 20 Fold:Four-helical up-and-down bundle Superfamily:Hemerythrin-like Family:Hemerythrin-like 45 d1aopa2 not modelled 7.7 29 Fold:Ferredoxin-like Superfamily:Nitrite/Sulfite reductase N-terminal domain-like Family:Duplicated SiR/NiR-like domains 1 and 3 46 d1a04a2 not modelled 7.4 18 Fold:Flavodoxin-like Superfamily:CheY-like Family:CheY-related 47 c3kt9A_ not modelled 7.2 11 PDB header:hydrolase Chain: A: PDB Molecule:aprataxin; PDBTitle: aprataxin fha domain 48 d1i4ya_ not modelled 7.2 20 Fold:Four-helical up-and-down bundle Superfamily:Hemerythrin-like Family:Hemerythrin-like 49 c1yj5C_ not modelled 7.1 14 PDB header:transferase Chain: C: PDB Molecule:5' polynucleotide kinase-3' phosphatase fha domain; PDBTitle: molecular architecture of mammalian polynucleotide kinase, a dna2 repair enzyme 50 d1dpea_ not modelled 7.0 23 Fold:Periplasmic binding protein-like II Superfamily:Periplasmic binding protein-like II Family:Phosphate binding protein-like 51 c3hzhA_ not modelled 7.0 19 PDB header:signaling protein Chain: A: PDB Molecule:chemotaxis response regulator (chey-3); PDBTitle: crystal structure of the chex-chey-bef3-mg+2 complex from2 borrelia burgdorferi 52 d1zlqa1 not modelled 6.9 17 Fold:Periplasmic binding protein-like II Superfamily:Periplasmic binding protein-like II Family:Phosphate binding protein-like 53 d1yjma1 not modelled 6.8 14 Fold:SMAD/FHA domain Superfamily:SMAD/FHA domain Family:FHA domain 54 c1ibaA_ not modelled 6.8 14 PDB header:phoshphotransferase Chain: A: PDB Molecule:glucose permease; PDBTitle: glucose permease (domain iib), nmr, 11 structures 55 d1us7b_ not modelled 6.5 38 Fold:Hsp90 co-chaperone CDC37 Superfamily:Hsp90 co-chaperone CDC37 Family:Hsp90 co-chaperone CDC37 56 c1us7B_ not modelled 6.5 38 PDB header:chaperone Chain: B: PDB Molecule:hsp90 co-chaperone cdc37; PDBTitle: complex of hsp90 and p50 57 d1fmfa_ not modelled 6.3 14 Fold:Flavodoxin-like Superfamily:Cobalamin (vitamin B12)-binding domain Family:Cobalamin (vitamin B12)-binding domain 58 d1mzha_ not modelled 6.2 17 Fold:TIM beta/alpha-barrel Superfamily:Aldolase Family:Class I aldolase 59 d1ccwa_ not modelled 6.2 14 Fold:Flavodoxin-like Superfamily:Cobalamin (vitamin B12)-binding domain Family:Cobalamin (vitamin B12)-binding domain 60 d1grja2 not modelled 6.1 23 Fold:FKBP-like Superfamily:FKBP-like Family:GreA transcript cleavage factor, C-terminal domain 61 c3nvaB_ not modelled 6.1 24 PDB header:ligase Chain: B: PDB Molecule:ctp synthase; PDBTitle: dimeric form of ctp synthase from sulfolobus solfataricus 62 d1m2ka_ not modelled 5.8 18 Fold:DHS-like NAD/FAD-binding domain Superfamily:DHS-like NAD/FAD-binding domain Family:Sir2 family of transcriptional regulators 63 d2hmza_ not modelled 5.8 18 Fold:Four-helical up-and-down bundle Superfamily:Hemerythrin-like Family:Hemerythrin-like 64 d1tiga_ not modelled 5.7 18 Fold:IF3-like Superfamily:Translation initiation factor IF3, C-terminal domain Family:Translation initiation factor IF3, C-terminal domain

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0