Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	Y	R	I	V	L	G	K	V	S	T	L	S	A	A	P	L	P	P	G	L	R	E	Q	A	P	Q	G	P	R	R	E	R	W	L	A	G	R	A	L	L	S	H	T	L	S	P	L	P	E	I	I	Y	G	E	Q	G	K	P	A
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?										?	?									?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		F	A	P	E	M	P	L	W	F	N	L	S	H	S	G	D	D	I	A	L	L	L	S	D	E	G	E	V	G	C	D	I	E	V	I	R	P	R	A	N	W	R	W	L	A	N	A	V	F	S	L	G	E	H	A	E	M	D	A	V
Secondary structure
SS confidence
Disorder																																						?			?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		H	P	D	Q	Q	L	E	M	F	W	R	I	W	T	R	K	E	A	I	V	K	Q	R	G	G	S	A	W	Q	I	V	S	V	D	S	T	Y	H	S	S	L	S	V	S	H	C	Q	L	E	N	L	S	L	A	I	C	T	P	T	P
Secondary structure
SS confidence
Disorder																																?	?	?	?	?			?		?								?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.
Sequence		F	T	L	T	A	D	S	V	Q	W	I	D	S	V	N
Secondary structure
SS confidence
Disorder													?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1qr0 chain A Region: 17 - 180 Aligned: 161 Modelled: 164 Confidence: 100.0% Identity: 28% PDB header:transferase Chain: A: PDB Molecule:4'-phosphopantetheinyl transferase sfp; PDBTitle: crystal structure of the 4'-phosphopantetheinyl transferase sfp-2 coenzyme a complex Phyre2 PDB 2cg5 chain A Region: 17 - 192 Aligned: 176 Modelled: 176 Confidence: 100.0% Identity: 22% PDB header:transferase/hydrolase Chain: A: PDB Molecule:l-aminoadipate-semialdehyde dehydrogenase- PDBTitle: structure of aminoadipate-semialdehyde dehydrogenase-2 phosphopantetheinyl transferase in complex with cytosolic3 acyl carrier protein and coenzyme a Phyre2 PDB 1qr0 chain A domain 2 Region: 87 - 180 Aligned: 92 Modelled: 94 Confidence: 99.8% Identity: 27% Fold: 4'-phosphopantetheinyl transferase Superfamily: 4'-phosphopantetheinyl transferase Family: 4'-Phosphopantetheinyl transferase SFP Phyre2 PDB 1qr0 chain A domain 1 Region: 1 - 84 Aligned: 84 Modelled: 84 Confidence: 99.6% Identity: 23% Fold: 4'-phosphopantetheinyl transferase Superfamily: 4'-phosphopantetheinyl transferase Family: 4'-Phosphopantetheinyl transferase SFP Phyre2 PDB 3hyk chain B Region: 88 - 177 Aligned: 90 Modelled: 90 Confidence: 99.4% Identity: 17% PDB header:transferase Chain: B: PDB Molecule:holo-[acyl-carrier-protein] synthase; PDBTitle: 2.31 angstrom resolution crystal structure of a holo-(acyl-carrier-2 protein) synthase from bacillus anthracis str. ames in complex with3 coa (3',5'-adp) Phyre2 PDB 2was chain A Region: 86 - 177 Aligned: 88 Modelled: 92 Confidence: 99.4% Identity: 24% PDB header:transferase Chain: A: PDB Molecule:3-oxoacyl-[acyl-carrier-protein] synthase; PDBTitle: structure of the fungal type i fas ppt domain Phyre2 PDB 1f7l chain A Region: 88 - 176 Aligned: 89 Modelled: 89 Confidence: 99.4% Identity: 19% Fold: 4'-phosphopantetheinyl transferase Superfamily: 4'-phosphopantetheinyl transferase Family: Holo-(acyl carrier protein) synthase ACPS Phyre2 PDB 1fth chain A Region: 88 - 177 Aligned: 88 Modelled: 90 Confidence: 99.3% Identity: 24% Fold: 4'-phosphopantetheinyl transferase Superfamily: 4'-phosphopantetheinyl transferase Family: Holo-(acyl carrier protein) synthase ACPS Phyre2 PDB 3ne9 chain B Region: 87 - 170 Aligned: 82 Modelled: 84 Confidence: 99.2% Identity: 18% PDB header:transferase Chain: B: PDB Molecule:phosphopantethiene protein transferase, ppt1p; PDBTitle: mycobacterium tuberculosis acyl carrier protein synthase adopts two2 different structural conformations: ph driven conformational changes3 of acps enzyme Phyre2 PDB 3f09 chain B Region: 88 - 177 Aligned: 90 Modelled: 90 Confidence: 99.2% Identity: 18% PDB header:transferase Chain: B: PDB Molecule:holo-[acyl-carrier-protein] synthase; PDBTitle: 1.82 angstrom resolution crystal structure of holo-(acyl-carrier-2 protein) synthase (acps) from staphylococcus aureus Phyre2 PDB 3h88 chain A Region: 88 - 152 Aligned: 63 Modelled: 65 Confidence: 99.1% Identity: 25% PDB header:transferase Chain: A: PDB Molecule:holo-[acyl-carrier-protein] synthase; PDBTitle: crystal structure of 4'-phosphopantetheinyl transferase acps from2 vibrio cholerae o1 biovar eltor Phyre2 PDB 2jbz chain A Region: 88 - 147 Aligned: 58 Modelled: 60 Confidence: 98.9% Identity: 22% PDB header:transferase Chain: A: PDB Molecule:holo-[acyl-carrier-protein] synthase; PDBTitle: crystal structure of the streptomyces coelicolor holo-2 [acyl-carrier-protein] synthase (acps) in complex with3 coenzyme a at 1.6 a Phyre2 PDB 2qg8 chain A Region: 88 - 150 Aligned: 61 Modelled: 63 Confidence: 98.6% Identity: 23% PDB header:transferase Chain: A: PDB Molecule:acyl carrier protein synthase py06285; PDBTitle: plasmodium yoelii acyl carrier protein synthase py06285 with adp bound Phyre2 PDB 3hqj chain A Region: 17 - 87 Aligned: 68 Modelled: 71 Confidence: 97.8% Identity: 28% PDB header:transferase Chain: A: PDB Molecule:holo-[acyl-carrier-protein] synthase; PDBTitle: structure-function analysis of mycobacterium tuberculosis2 acyl carrier protein synthase (acps). Phyre2 PDB 2ch4 chain A Region: 57 - 111 Aligned: 51 Modelled: 52 Confidence: 61.3% Identity: 31% PDB header:transferase/chemotaxis Chain: A: PDB Molecule:chemotaxis protein chea; PDBTitle: complex between bacterial chemotaxis histidine kinase chea2 domains p4 and p5 and receptor-adaptor protein chew Phyre2 PDB 2bu8 chain A Region: 38 - 110 Aligned: 66 Modelled: 73 Confidence: 46.8% Identity: 20% PDB header:transferase Chain: A: PDB Molecule:pyruvate dehydrogenase kinase isoenzyme 2; PDBTitle: crystal structures of human pyruvate dehydrogenase kinase 22 containing physiological and synthetic ligands Phyre2 PDB 2q8f chain A Region: 62 - 110 Aligned: 42 Modelled: 49 Confidence: 44.7% Identity: 24% PDB header:transferase Chain: A: PDB Molecule:[pyruvate dehydrogenase [lipoamide]] kinase isozyme 1; PDBTitle: structure of pyruvate dehydrogenase kinase isoform 1 Phyre2 PDB 1y8o chain A Region: 63 - 110 Aligned: 41 Modelled: 48 Confidence: 44.1% Identity: 22% PDB header:transferase Chain: A: PDB Molecule:[pyruvate dehydrogenase [lipoamide]] kinase isozyme 3; PDBTitle: crystal structure of the pdk3-l2 complex Phyre2 PDB 3d2r chain B Region: 62 - 110 Aligned: 42 Modelled: 49 Confidence: 39.9% Identity: 21% PDB header:transferase Chain: B: PDB Molecule:[pyruvate dehydrogenase [lipoamide]] kinase isozyme 4; PDBTitle: crystal structure of pyruvate dehydrogenase kinase isoform 4 in2 complex with adp Phyre2 PDB 3k8a chain A Region: 45 - 77 Aligned: 27 Modelled: 33 Confidence: 33.5% Identity: 26% PDB header:dna binding protein Chain: A: PDB Molecule:putative primosomal replication protein; PDBTitle: neisseria gonorrhoeae prib Phyre2 1 c1qr0A_ 2 c2cg5A_ 3 d1qr0a2 4 d1qr0a1 5 c3hykB_ 6 c2wasA_ 7 d1f7la_ 8 d1ftha_ 9 c3ne9B_ 10 c3f09B_ 11 c3h88A_ 12 c2jbzA_ 13 c2qg8A_ 14 c3hqjA_ 15 c2ch4A_ 16 c2bu8A_ 17 c2q8fA_ 18 c1y8oA_ 19 c3d2rB_ 20 c3k8aA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c1qr0A_ 100.0 28 PDB header:transferase Chain: A: PDB Molecule:4'-phosphopantetheinyl transferase sfp; PDBTitle: crystal structure of the 4'-phosphopantetheinyl transferase sfp-2 coenzyme a complex 2 c2cg5A_ 100.0 22 PDB header:transferase/hydrolase Chain: A: PDB Molecule:l-aminoadipate-semialdehyde dehydrogenase- PDBTitle: structure of aminoadipate-semialdehyde dehydrogenase-2 phosphopantetheinyl transferase in complex with cytosolic3 acyl carrier protein and coenzyme a 3 d1qr0a2 99.8 27 Fold:4'-phosphopantetheinyl transferase Superfamily:4'-phosphopantetheinyl transferase Family:4'-Phosphopantetheinyl transferase SFP 4 d1qr0a1 99.6 23 Fold:4'-phosphopantetheinyl transferase Superfamily:4'-phosphopantetheinyl transferase Family:4'-Phosphopantetheinyl transferase SFP 5 c3hykB_ 99.4 17 PDB header:transferase Chain: B: PDB Molecule:holo-[acyl-carrier-protein] synthase; PDBTitle: 2.31 angstrom resolution crystal structure of a holo-(acyl-carrier-2 protein) synthase from bacillus anthracis str. ames in complex with3 coa (3',5'-adp) 6 c2wasA_ 99.4 24 PDB header:transferase Chain: A: PDB Molecule:3-oxoacyl-[acyl-carrier-protein] synthase; PDBTitle: structure of the fungal type i fas ppt domain 7 d1f7la_ 99.4 19 Fold:4'-phosphopantetheinyl transferase Superfamily:4'-phosphopantetheinyl transferase Family:Holo-(acyl carrier protein) synthase ACPS 8 d1ftha_ 99.3 24 Fold:4'-phosphopantetheinyl transferase Superfamily:4'-phosphopantetheinyl transferase Family:Holo-(acyl carrier protein) synthase ACPS 9 c3ne9B_ 99.2 18 PDB header:transferase Chain: B: PDB Molecule:phosphopantethiene protein transferase, ppt1p; PDBTitle: mycobacterium tuberculosis acyl carrier protein synthase adopts two2 different structural conformations: ph driven conformational changes3 of acps enzyme 10 c3f09B_ 99.2 18 PDB header:transferase Chain: B: PDB Molecule:holo-[acyl-carrier-protein] synthase; PDBTitle: 1.82 angstrom resolution crystal structure of holo-(acyl-carrier-2 protein) synthase (acps) from staphylococcus aureus 11 c3h88A_ 99.1 25 PDB header:transferase Chain: A: PDB Molecule:holo-[acyl-carrier-protein] synthase; PDBTitle: crystal structure of 4'-phosphopantetheinyl transferase acps from2 vibrio cholerae o1 biovar eltor 12 c2jbzA_ 98.9 22 PDB header:transferase Chain: A: PDB Molecule:holo-[acyl-carrier-protein] synthase; PDBTitle: crystal structure of the streptomyces coelicolor holo-2 [acyl-carrier-protein] synthase (acps) in complex with3 coenzyme a at 1.6 a 13 c2qg8A_ 98.6 23 PDB header:transferase Chain: A: PDB Molecule:acyl carrier protein synthase py06285; PDBTitle: plasmodium yoelii acyl carrier protein synthase py06285 with adp bound 14 c3hqjA_ 97.8 28 PDB header:transferase Chain: A: PDB Molecule:holo-[acyl-carrier-protein] synthase; PDBTitle: structure-function analysis of mycobacterium tuberculosis2 acyl carrier protein synthase (acps). 15 c2ch4A_ 61.3 31 PDB header:transferase/chemotaxis Chain: A: PDB Molecule:chemotaxis protein chea; PDBTitle: complex between bacterial chemotaxis histidine kinase chea2 domains p4 and p5 and receptor-adaptor protein chew 16 c2bu8A_ 46.8 20 PDB header:transferase Chain: A: PDB Molecule:pyruvate dehydrogenase kinase isoenzyme 2; PDBTitle: crystal structures of human pyruvate dehydrogenase kinase 22 containing physiological and synthetic ligands 17 c2q8fA_ 44.7 24 PDB header:transferase Chain: A: PDB Molecule:[pyruvate dehydrogenase [lipoamide]] kinase isozyme 1; PDBTitle: structure of pyruvate dehydrogenase kinase isoform 1 18 c1y8oA_ 44.1 22 PDB header:transferase Chain: A: PDB Molecule:[pyruvate dehydrogenase [lipoamide]] kinase isozyme 3; PDBTitle: crystal structure of the pdk3-l2 complex 19 c3d2rB_ 39.9 21 PDB header:transferase Chain: B: PDB Molecule:[pyruvate dehydrogenase [lipoamide]] kinase isozyme 4; PDBTitle: crystal structure of pyruvate dehydrogenase kinase isoform 4 in2 complex with adp 20 c3k8aA_ 33.5 26 PDB header:dna binding protein Chain: A: PDB Molecule:putative primosomal replication protein; PDBTitle: neisseria gonorrhoeae prib 21 c3en2A_ not modelled 23.6 32 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:probable primosomal replication protein n; PDBTitle: three-dimensional structure of the protein prib from2 ralstonia solanacearum at the resolution 2.3a. northeast3 structural genomics consortium target rsr213c. 22 d1txya_ not modelled 19.5 22 Fold:OB-fold Superfamily:Nucleic acid-binding proteins Family:Single strand DNA-binding domain, SSB 23 c3fhwB_ not modelled 18.2 24 PDB header:dna binding protein Chain: B: PDB Molecule:primosomal replication protein n; PDBTitle: crystal structure of the protein prib from bordetella parapertussis.2 northeast structural genomics consortium target bpr162. 24 d1v1qa_ not modelled 14.4 22 Fold:OB-fold Superfamily:Nucleic acid-binding proteins Family:Single strand DNA-binding domain, SSB 25 d1i58a_ not modelled 13.0 20 Fold:ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase Superfamily:ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase Family:Histidine kinase 26 c2z0rA_ not modelled 12.3 33 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:putative uncharacterized protein ttha0547; PDBTitle: crystal structure of hypothetical protein ttha0547 27 d1pfoa_ not modelled 11.4 34 Fold:Perfringolysin Superfamily:Perfringolysin Family:Perfringolysin 28 c1pfoA_ not modelled 11.4 34 PDB header:toxin Chain: A: PDB Molecule:perfringolysin o; PDBTitle: perfringolysin o 29 c2j8pA_ not modelled 8.8 31 PDB header:nuclear protein Chain: A: PDB Molecule:cleavage stimulation factor 64 kda subunit; PDBTitle: nmr structure of c-terminal domain of human cstf-64 30 d1s6la1 not modelled 7.9 17 Fold:DNA/RNA-binding 3-helical bundle Superfamily:"Winged helix" DNA-binding domain Family:MerB N-terminal domain-like 31 d1y8oa2 not modelled 7.8 17 Fold:ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase Superfamily:ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase Family:alpha-ketoacid dehydrogenase kinase, C-terminal domain 32 d1knxa1 not modelled 7.7 12 Fold:MurF and HprK N-domain-like Superfamily:HprK N-terminal domain-like Family:HPr kinase/phoshatase HprK N-terminal domain 33 d2a13a1 not modelled 7.0 24 Fold:Lipocalins Superfamily:Lipocalins Family:Rv2717c-like 34 c1zy7A_ not modelled 6.9 23 PDB header:hydrolase Chain: A: PDB Molecule:rna-specific adenosine deaminase b1, isoform PDBTitle: crystal structure of the catalytic domain of an adenosine2 deaminase that acts on rna (hadar2) bound to inositol3 hexakisphosphate (ihp) 35 d2yt9a1 not modelled 5.7 29 Fold:beta-beta-alpha zinc fingers Superfamily:beta-beta-alpha zinc fingers Family:Classic zinc finger, C2H2 36 d1zq1a1 not modelled 5.4 35 Fold:Sm-like fold Superfamily:GatD N-terminal domain-like Family:GatD N-terminal domain-like 37 c2knjA_ not modelled 5.2 14 PDB header:antimicrobial protein Chain: A: PDB Molecule:microplusin preprotein; PDBTitle: nmr structure of microplusin a antimicrobial peptide from2 rhipicephalus (boophilus) microplus

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0