Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	S	E	S	L	S	W	M	Q	T	G	D	T	L	A	L	S	G	E	L	D	Q	D	V	L	L	P	L	W	E	M	R	E	E	A	V	K	G	I	T	C	I	D	L	S	R	V	S	R	V	D	T	G	G	L	A	L	L	L	H
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?																													?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.
Sequence		L	I	D	L	A	K	K	Q	G	N	N	V	T	L	Q	G	V	N	D	K	V	Y	T	L	A	K	L	Y	N	L	P	A	D	V	L	P	R
Secondary structure
SS confidence
Disorder																																			?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1th8 chain B Region: 1 - 96 Aligned: 96 Modelled: 96 Confidence: 99.7% Identity: 23% Fold: SpoIIaa-like Superfamily: SpoIIaa-like Family: Anti-sigma factor antagonist SpoIIaa Phyre2 PDB 3t6o chain A Region: 2 - 96 Aligned: 94 Modelled: 95 Confidence: 99.7% Identity: 13% PDB header:transport protein Chain: A: PDB Molecule:sulfate transporter/antisigma-factor antagonist stas; PDBTitle: the structure of an anti-sigma-factor antagonist (stas) domain protein2 from planctomyces limnophilus. Phyre2 PDB 1vc1 chain A Region: 1 - 94 Aligned: 94 Modelled: 94 Confidence: 99.7% Identity: 22% Fold: SpoIIaa-like Superfamily: SpoIIaa-like Family: Anti-sigma factor antagonist SpoIIaa Phyre2 PDB 3f43 chain A Region: 1 - 96 Aligned: 96 Modelled: 96 Confidence: 99.7% Identity: 17% PDB header:transcription Chain: A: PDB Molecule:putative anti-sigma factor antagonist tm1081; PDBTitle: crystal structure of a putative anti-sigma factor antagonist (tm1081)2 from thermotoga maritima at 1.59 a resolution Phyre2 PDB 1auz chain A Region: 1 - 96 Aligned: 96 Modelled: 96 Confidence: 99.7% Identity: 19% Fold: SpoIIaa-like Superfamily: SpoIIaa-like Family: Anti-sigma factor antagonist SpoIIaa Phyre2 PDB 1h4x chain A Region: 7 - 94 Aligned: 87 Modelled: 88 Confidence: 99.6% Identity: 16% Fold: SpoIIaa-like Superfamily: SpoIIaa-like Family: Anti-sigma factor antagonist SpoIIaa Phyre2 PDB 3mgl chain A Region: 13 - 96 Aligned: 84 Modelled: 84 Confidence: 99.6% Identity: 20% PDB header:transport protein Chain: A: PDB Molecule:sulfate permease family protein; PDBTitle: crystal structure of permease family protein from vibrio2 cholerae Phyre2 PDB 3oir chain A Region: 2 - 94 Aligned: 93 Modelled: 93 Confidence: 99.5% Identity: 18% PDB header:transport protein Chain: A: PDB Molecule:sulfate transporter sulfate transporter family protein; PDBTitle: crystal structure of sulfate transporter family protein from wolinella2 succinogenes Phyre2 PDB 2vy9 chain A Region: 5 - 91 Aligned: 87 Modelled: 86 Confidence: 99.5% Identity: 18% PDB header:gene regulation Chain: A: PDB Molecule:anti-sigma-factor antagonist; PDBTitle: molecular architecture of the stressosome, a signal2 integration and transduction hub Phyre2 PDB 2kln chain A Region: 13 - 96 Aligned: 84 Modelled: 84 Confidence: 99.5% Identity: 12% PDB header:transport protein Chain: A: PDB Molecule:probable sulphate-transport transmembrane protein, cog0659; PDBTitle: solution structure of stas domain of rv1739c from m. tuberculosis Phyre2 PDB 3lkl chain B Region: 9 - 86 Aligned: 78 Modelled: 78 Confidence: 99.4% Identity: 19% PDB header:transport protein Chain: B: PDB Molecule:antisigma-factor antagonist stas; PDBTitle: crystal structure of the c-terminal domain of anti-sigma factor2 antagonist stas from rhodobacter sphaeroides Phyre2 PDB 3ny7 chain A Region: 13 - 96 Aligned: 83 Modelled: 84 Confidence: 99.3% Identity: 17% PDB header:membrane protein Chain: A: PDB Molecule:sulfate transporter; PDBTitle: stas domain of ychm bound to acp Phyre2 PDB 3llo chain A Region: 13 - 94 Aligned: 78 Modelled: 82 Confidence: 99.2% Identity: 14% PDB header:motor protein Chain: A: PDB Molecule:prestin; PDBTitle: crystal structure of the stas domain of motor protein prestin (anion2 transporter slc26a5) Phyre2 PDB 3ih9 chain A Region: 7 - 79 Aligned: 73 Modelled: 73 Confidence: 95.6% Identity: 19% PDB header:hydrolase Chain: A: PDB Molecule:salt-tolerant glutaminase; PDBTitle: crystal structure analysis of mglu in its tris form Phyre2 PDB 3c9u chain A domain 2 Region: 50 - 91 Aligned: 41 Modelled: 42 Confidence: 25.7% Identity: 17% Fold: PurM C-terminal domain-like Superfamily: PurM C-terminal domain-like Family: PurM C-terminal domain-like Phyre2 PDB 2q3l chain A domain 1 Region: 1 - 89 Aligned: 89 Modelled: 89 Confidence: 18.5% Identity: 18% Fold: SpoIIaa-like Superfamily: SpoIIaa-like Family: Sfri0576-like Phyre2 PDB 3ghf chain A Region: 19 - 90 Aligned: 67 Modelled: 72 Confidence: 14.0% Identity: 12% PDB header:cell cycle Chain: A: PDB Molecule:septum site-determining protein minc; PDBTitle: crystal structure of the septum site-determining protein2 minc from salmonella typhimurium Phyre2 PDB 3bez chain C Region: 13 - 76 Aligned: 64 Modelled: 64 Confidence: 12.1% Identity: 20% PDB header:hydrolase Chain: C: PDB Molecule:protease 4; PDBTitle: crystal structure of escherichia coli signal peptide peptidase (sppa),2 semet crystals Phyre2 PDB 2fug chain 6 domain 1 Region: 9 - 44 Aligned: 36 Modelled: 36 Confidence: 10.3% Identity: 14% Fold: HydA/Nqo6-like Superfamily: HydA/Nqo6-like Family: Nq06-like Phyre2 PDB 2pr7 chain A Region: 40 - 92 Aligned: 53 Modelled: 53 Confidence: 8.3% Identity: 13% PDB header:hydrolase Chain: A: PDB Molecule:haloacid dehalogenase/epoxide hydrolase family; PDBTitle: crystal structure of uncharacterized protein (np_599989.1) from2 corynebacterium glutamicum atcc 13032 kitasato at 1.44 a resolution Phyre2 1 d1th8b_ 2 c3t6oA_ 3 d1vc1a_ 4 c3f43A_ 5 d1auza_ 6 d1h4xa_ 7 c3mglA_ 8 c3oirA_ 9 c2vy9A_ 10 c2klnA_ 11 c3lklB_ 12 c3ny7A_ 13 c3lloA_ 14 c3ih9A_ 15 d3c9ua2 16 d2q3la1 17 c3ghfA_ 18 c3bezC_ 19 d2fug61 20 c2pr7A_ 21 22

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1th8b_ 99.7 23 Fold:SpoIIaa-like Superfamily:SpoIIaa-like Family:Anti-sigma factor antagonist SpoIIaa 2 c3t6oA_ 99.7 13 PDB header:transport protein Chain: A: PDB Molecule:sulfate transporter/antisigma-factor antagonist stas; PDBTitle: the structure of an anti-sigma-factor antagonist (stas) domain protein2 from planctomyces limnophilus. 3 d1vc1a_ 99.7 22 Fold:SpoIIaa-like Superfamily:SpoIIaa-like Family:Anti-sigma factor antagonist SpoIIaa 4 c3f43A_ 99.7 17 PDB header:transcription Chain: A: PDB Molecule:putative anti-sigma factor antagonist tm1081; PDBTitle: crystal structure of a putative anti-sigma factor antagonist (tm1081)2 from thermotoga maritima at 1.59 a resolution 5 d1auza_ 99.7 19 Fold:SpoIIaa-like Superfamily:SpoIIaa-like Family:Anti-sigma factor antagonist SpoIIaa 6 d1h4xa_ 99.6 16 Fold:SpoIIaa-like Superfamily:SpoIIaa-like Family:Anti-sigma factor antagonist SpoIIaa 7 c3mglA_ 99.6 20 PDB header:transport protein Chain: A: PDB Molecule:sulfate permease family protein; PDBTitle: crystal structure of permease family protein from vibrio2 cholerae 8 c3oirA_ 99.5 18 PDB header:transport protein Chain: A: PDB Molecule:sulfate transporter sulfate transporter family protein; PDBTitle: crystal structure of sulfate transporter family protein from wolinella2 succinogenes 9 c2vy9A_ 99.5 18 PDB header:gene regulation Chain: A: PDB Molecule:anti-sigma-factor antagonist; PDBTitle: molecular architecture of the stressosome, a signal2 integration and transduction hub 10 c2klnA_ 99.5 12 PDB header:transport protein Chain: A: PDB Molecule:probable sulphate-transport transmembrane protein, cog0659; PDBTitle: solution structure of stas domain of rv1739c from m. tuberculosis 11 c3lklB_ 99.4 19 PDB header:transport protein Chain: B: PDB Molecule:antisigma-factor antagonist stas; PDBTitle: crystal structure of the c-terminal domain of anti-sigma factor2 antagonist stas from rhodobacter sphaeroides 12 c3ny7A_ 99.3 17 PDB header:membrane protein Chain: A: PDB Molecule:sulfate transporter; PDBTitle: stas domain of ychm bound to acp 13 c3lloA_ 99.2 14 PDB header:motor protein Chain: A: PDB Molecule:prestin; PDBTitle: crystal structure of the stas domain of motor protein prestin (anion2 transporter slc26a5) 14 c3ih9A_ 95.6 19 PDB header:hydrolase Chain: A: PDB Molecule:salt-tolerant glutaminase; PDBTitle: crystal structure analysis of mglu in its tris form 15 d3c9ua2 25.7 17 Fold:PurM C-terminal domain-like Superfamily:PurM C-terminal domain-like Family:PurM C-terminal domain-like 16 d2q3la1 18.5 18 Fold:SpoIIaa-like Superfamily:SpoIIaa-like Family:Sfri0576-like 17 c3ghfA_ 14.0 12 PDB header:cell cycle Chain: A: PDB Molecule:septum site-determining protein minc; PDBTitle: crystal structure of the septum site-determining protein2 minc from salmonella typhimurium 18 c3bezC_ 12.1 20 PDB header:hydrolase Chain: C: PDB Molecule:protease 4; PDBTitle: crystal structure of escherichia coli signal peptide peptidase (sppa),2 semet crystals 19 d2fug61 10.3 14 Fold:HydA/Nqo6-like Superfamily:HydA/Nqo6-like Family:Nq06-like 20 c2pr7A_ 8.3 13 PDB header:hydrolase Chain: A: PDB Molecule:haloacid dehalogenase/epoxide hydrolase family; PDBTitle: crystal structure of uncharacterized protein (np_599989.1) from2 corynebacterium glutamicum atcc 13032 kitasato at 1.44 a resolution 21 c2k42A_ not modelled 5.2 5 PDB header:signaling protein Chain: A: PDB Molecule:wiskott-aldrich syndrome protein; PDBTitle: solution structure of the gtpase binding domain of wasp in2 complex with espfu, an ehec effector 22 d1xbta1 not modelled 5.1 10 Fold:P-loop containing nucleoside triphosphate hydrolases Superfamily:P-loop containing nucleoside triphosphate hydrolases Family:Type II thymidine kinase

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

---

Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0