Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	S	G	E	K	K	A	K	G	W	R	F	Y	G	L	V	G	F	G	A	I	A	L	L	S	A	G	V	W	A	L	Q	Y	A	G	S	G	P	E	K	T	L	S	P	L	V	V	H	N	N	L	Q	I	D	L	N	E	P	D	L
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?																				?		?	?	?				?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		F	L	D	S	D	S	L	S	Q	L	P	K	D	L	L	T	I	P	F	L	H	D	V	L	S	E	D	F	V	F	Y	Y	Q	N	H	A	D	R	L	G	I	E	G	S	I	R	R	I	V	Y	E	H	D	L	T	L	K	D	K	L
Secondary structure
SS confidence
Disorder		?	?	?		?	?																																	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		F	S	S	L	L	D	Q	P	A	Q	A	A	L	W	H	D	K	Q	G	H	L	S	H	Y	M	V	L	I	Q	R	S	G	L	S	K	L	L	E	P	L	L	F	A	A	T	S	D	S	Q	L	S	K	T	E	I	S	S	I	K	I
Secondary structure
SS confidence
Disorder																?		?																									?	?	?		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		N	S	E	T	V	P	V	Y	Q	L	R	Y	N	G	N	N	A	L	M	F	A	T	Y	Q	D	K	M	L	V	F	S	S	T	D	M	L	F	K	D	D	Q	Q	D	T	E	A	T	A	I	A	G	D	L	L	S	G	K	K	R	W
Secondary structure
SS confidence
Disorder		?																																					?		?	?	?	?	?	?	?	?	?	?		?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		Q	A	S	F	G	L	E	E	R	T	A	E	K	T	P	V	R	Q	R	I	V	V	S	A	R	W	L	G	F	G	Y	Q	R	L	M	P	S	F	A	G	V	R	F	E	M	G	N	D	G	W	H	S	F	V	A	L	N	D	E	S
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?																																										?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		A	S	V	D	A	S	F	D	F	T	P	V	W	N	S	M	P	A	G	A	S	F	C	V	A	V	P	Y	S	H	G	I	A	E	E	M	L	S	H	I	S	Q	E	N	D	K	L	N	G	A	L	D	G	A	A	G	L	C	W	Y
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?																								?	?															?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		E	D	S	K	L	Q	T	P	L	F	V	G	Q	F	D	G	T	A	E	Q	A	Q	L	P	G	K	L	F	T	Q	N	I	G	A	H	E	S	K	A	P	E	G	V	L	P	V	S	Q	T	Q	Q	G	E	A	Q	I	W	R	R	E
Secondary structure
SS confidence
Disorder														?	?	?	?	?	?	?	?	?	?											?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		V	S	S	R	Y	G	Q	Y	P	K	A	Q	A	A	Q	P	D	Q	L	M	S	D	Y	F	F	R	V	S	L	A	M	Q	N	K	T	L	L	F	S	L	D	D	T	L	V	N	N	A	L	Q	T	L	N	K	T	R	P	A	M	V
Secondary structure
SS confidence
Disorder			?			?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?																															?		?			?
Disorder confidence
		.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.	510	.	.	.	.	.	.	.	.	.	520	.	.	.	.	.	.	.	.	.	530	.	.	.	.	.	.	.	.	.	540
Sequence		D	V	I	P	T	D	G	I	V	P	L	Y	I	N	P	Q	G	I	A	K	L	L	R	N	E	T	L	T	S	L	P	K	N	L	E	P	V	F	Y	N	A	A	Q	T	L	L	M	P	K	L	D	A	L	S	Q	Q	P	R	Y	V
Secondary structure
SS confidence
Disorder		?	?																											?	?																							?
Disorder confidence
		.	.	.	.	.	.	.	.	.	550	.	.	.	.	.	.	.	.	.	560	.	.
Sequence		M	K	L	A	Q	M	E	P	G	A	A	W	Q	W	L	P	I	T	W	Q	P	L
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?									?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1b24 chain A domain 1 Region: 187 - 197 Aligned: 11 Modelled: 11 Confidence: 26.8% Identity: 45% Fold: Homing endonuclease-like Superfamily: Homing endonucleases Family: Group I mobile intron endonuclease Phyre2 PDB 2pia chain A domain 3 Region: 433 - 470 Aligned: 38 Modelled: 38 Confidence: 26.3% Identity: 11% Fold: beta-Grasp (ubiquitin-like) Superfamily: 2Fe-2S ferredoxin-like Family: 2Fe-2S ferredoxin domains from multidomain proteins Phyre2 PDB 1cwe chain A Region: 86 - 107 Aligned: 20 Modelled: 22 Confidence: 22.8% Identity: 30% Fold: SH2-like Superfamily: SH2 domain Family: SH2 domain Phyre2 PDB 2afj chain A domain 1 Region: 415 - 493 Aligned: 77 Modelled: 79 Confidence: 15.1% Identity: 21% Fold: Concanavalin A-like lectins/glucanases Superfamily: Concanavalin A-like lectins/glucanases Family: SPRY domain Phyre2 PDB 2z4d chain A Region: 163 - 200 Aligned: 32 Modelled: 32 Confidence: 14.4% Identity: 13% PDB header:nuclear protein Chain: A: PDB Molecule:26s proteasome regulatory subunit rpn13; PDBTitle: nmr structures of yeast proteasome component rpn13 Phyre2 PDB 3ts3 chain D Region: 277 - 316 Aligned: 40 Modelled: 40 Confidence: 10.1% Identity: 13% PDB header:viral protein Chain: D: PDB Molecule:capsid polyprotein; PDBTitle: crystal structure of the projection domain of the turkey astrovirus2 capsid protein at 1.5 angstrom resolution Phyre2 PDB 3uat chain A Region: 275 - 307 Aligned: 32 Modelled: 33 Confidence: 9.8% Identity: 28% PDB header:peptide binding protein Chain: A: PDB Molecule:disks large homolog 1; PDBTitle: guanylate kinase domains of the maguk family scaffold proteins as2 specific phospho-protein binding modules Phyre2 PDB 2w9v chain A Region: 410 - 425 Aligned: 16 Modelled: 16 Confidence: 9.3% Identity: 38% PDB header:toxin Chain: A: PDB Molecule:short disintegrin jerdostatin; PDBTitle: solution structure of jerdostatin from trimeresurus2 jerdonii with end c-terminal residues n45g46 deleted Phyre2 PDB 2i46 chain A Region: 80 - 116 Aligned: 37 Modelled: 37 Confidence: 8.9% Identity: 11% PDB header:protein binding Chain: A: PDB Molecule:adrenocortical dysplasia protein homolog; PDBTitle: crystal structure of human tpp1 Phyre2 PDB 1eg3 chain A domain 2 Region: 55 - 134 Aligned: 70 Modelled: 80 Confidence: 8.6% Identity: 26% Fold: EF Hand-like Superfamily: EF-hand Family: EF-hand modules in multidomain proteins Phyre2 PDB 1rku chain A Region: 454 - 476 Aligned: 23 Modelled: 23 Confidence: 8.5% Identity: 13% Fold: HAD-like Superfamily: HAD-like Family: Homoserine kinase ThrH Phyre2 PDB 2b6c chain A domain 1 Region: 465 - 512 Aligned: 39 Modelled: 48 Confidence: 7.9% Identity: 26% Fold: alpha-alpha superhelix Superfamily: ARM repeat Family: BC3264-like Phyre2 PDB 1y0j chain A domain 1 Region: 310 - 325 Aligned: 16 Modelled: 16 Confidence: 7.3% Identity: 38% Fold: Glucocorticoid receptor-like (DNA-binding domain) Superfamily: Glucocorticoid receptor-like (DNA-binding domain) Family: Erythroid transcription factor GATA-1 Phyre2 PDB 2p11 chain A Region: 452 - 467 Aligned: 16 Modelled: 16 Confidence: 7.2% Identity: 38% PDB header:hydrolase Chain: A: PDB Molecule:hypothetical protein; PDBTitle: crystal structure of a putative haloacid dehalogenase-like hydrolase2 (bxe_b1342) from burkholderia xenovorans lb400 at 2.20 a resolution Phyre2 PDB 3u7z chain A Region: 270 - 306 Aligned: 36 Modelled: 37 Confidence: 7.1% Identity: 11% PDB header:metal binding protein Chain: A: PDB Molecule:putative metal binding protein rumgna_00854; PDBTitle: crystal structure of a putative metal binding protein rumgna_008542 (zp_02040092.1) from ruminococcus gnavus atcc 29149 at 1.30 a3 resolution Phyre2 PDB 1a3q chain A domain 1 Region: 283 - 309 Aligned: 27 Modelled: 27 Confidence: 7.1% Identity: 19% Fold: Immunoglobulin-like beta-sandwich Superfamily: E set domains Family: NF-kappa-B/REL/DORSAL transcription factors, C-terminal domain Phyre2 PDB 2f69 chain A domain 1 Region: 356 - 360 Aligned: 5 Modelled: 5 Confidence: 7.1% Identity: 60% Fold: open-sided beta-meander Superfamily: Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain Family: Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain Phyre2 PDB 2nn6 chain G domain 3 Region: 441 - 491 Aligned: 44 Modelled: 51 Confidence: 7.0% Identity: 27% Fold: Eukaryotic type KH-domain (KH-domain type I) Superfamily: Eukaryotic type KH-domain (KH-domain type I) Family: Eukaryotic type KH-domain (KH-domain type I) Phyre2 PDB 3m9b chain K Region: 142 - 213 Aligned: 71 Modelled: 71 Confidence: 6.9% Identity: 10% PDB header:chaperone Chain: K: PDB Molecule:proteasome-associated atpase; PDBTitle: crystal structure of the amino terminal coiled coil domain and the2 inter domain of the mycobacterium tuberculosis proteasomal atpase mpa Phyre2 PDB 1q90 chain R Region: 267 - 275 Aligned: 9 Modelled: 9 Confidence: 6.9% Identity: 33% PDB header:photosynthesis Chain: R: PDB Molecule:cytochrome b6-f complex iron-sulfur subunit; PDBTitle: structure of the cytochrome b6f (plastohydroquinone : plastocyanin2 oxidoreductase) from chlamydomonas reinhardtii Phyre2 1 d1b24a1 2 d2piaa3 3 d1cwea_ 4 d2afja1 5 c2z4dA_ 6 c3ts3D_ 7 c3uatA_ 8 c2w9vA_ 9 c2i46A_ 10 d1eg3a2 11 d1rkua_ 12 d2b6ca1 13 d1y0ja1 14 c2p11A_ 15 c3u7zA_ 16 d1a3qa1 17 d2f69a1 18 d2nn6g3 19 c3m9bK_ 20 c1q90R_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1b24a1 26.8 45 Fold:Homing endonuclease-like Superfamily:Homing endonucleases Family:Group I mobile intron endonuclease 2 d2piaa3 26.3 11 Fold:beta-Grasp (ubiquitin-like) Superfamily:2Fe-2S ferredoxin-like Family:2Fe-2S ferredoxin domains from multidomain proteins 3 d1cwea_ 22.8 30 Fold:SH2-like Superfamily:SH2 domain Family:SH2 domain 4 d2afja1 15.1 21 Fold:Concanavalin A-like lectins/glucanases Superfamily:Concanavalin A-like lectins/glucanases Family:SPRY domain 5 c2z4dA_ 14.4 13 PDB header:nuclear protein Chain: A: PDB Molecule:26s proteasome regulatory subunit rpn13; PDBTitle: nmr structures of yeast proteasome component rpn13 6 c3ts3D_ 10.1 13 PDB header:viral protein Chain: D: PDB Molecule:capsid polyprotein; PDBTitle: crystal structure of the projection domain of the turkey astrovirus2 capsid protein at 1.5 angstrom resolution 7 c3uatA_ 9.8 28 PDB header:peptide binding protein Chain: A: PDB Molecule:disks large homolog 1; PDBTitle: guanylate kinase domains of the maguk family scaffold proteins as2 specific phospho-protein binding modules 8 c2w9vA_ 9.3 38 PDB header:toxin Chain: A: PDB Molecule:short disintegrin jerdostatin; PDBTitle: solution structure of jerdostatin from trimeresurus2 jerdonii with end c-terminal residues n45g46 deleted 9 c2i46A_ 8.9 11 PDB header:protein binding Chain: A: PDB Molecule:adrenocortical dysplasia protein homolog; PDBTitle: crystal structure of human tpp1 10 d1eg3a2 8.6 26 Fold:EF Hand-like Superfamily:EF-hand Family:EF-hand modules in multidomain proteins 11 d1rkua_ 8.5 13 Fold:HAD-like Superfamily:HAD-like Family:Homoserine kinase ThrH 12 d2b6ca1 7.9 26 Fold:alpha-alpha superhelix Superfamily:ARM repeat Family:BC3264-like 13 d1y0ja1 7.3 38 Fold:Glucocorticoid receptor-like (DNA-binding domain) Superfamily:Glucocorticoid receptor-like (DNA-binding domain) Family:Erythroid transcription factor GATA-1 14 c2p11A_ 7.2 38 PDB header:hydrolase Chain: A: PDB Molecule:hypothetical protein; PDBTitle: crystal structure of a putative haloacid dehalogenase-like hydrolase2 (bxe_b1342) from burkholderia xenovorans lb400 at 2.20 a resolution 15 c3u7zA_ 7.1 11 PDB header:metal binding protein Chain: A: PDB Molecule:putative metal binding protein rumgna_00854; PDBTitle: crystal structure of a putative metal binding protein rumgna_008542 (zp_02040092.1) from ruminococcus gnavus atcc 29149 at 1.30 a3 resolution 16 d1a3qa1 7.1 19 Fold:Immunoglobulin-like beta-sandwich Superfamily:E set domains Family:NF-kappa-B/REL/DORSAL transcription factors, C-terminal domain 17 d2f69a1 7.1 60 Fold:open-sided beta-meander Superfamily:Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain Family:Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain 18 d2nn6g3 7.0 27 Fold:Eukaryotic type KH-domain (KH-domain type I) Superfamily:Eukaryotic type KH-domain (KH-domain type I) Family:Eukaryotic type KH-domain (KH-domain type I) 19 c3m9bK_ 6.9 10 PDB header:chaperone Chain: K: PDB Molecule:proteasome-associated atpase; PDBTitle: crystal structure of the amino terminal coiled coil domain and the2 inter domain of the mycobacterium tuberculosis proteasomal atpase mpa 20 c1q90R_ 6.9 33 PDB header:photosynthesis Chain: R: PDB Molecule:cytochrome b6-f complex iron-sulfur subunit; PDBTitle: structure of the cytochrome b6f (plastohydroquinone : plastocyanin2 oxidoreductase) from chlamydomonas reinhardtii 21 d1q90r_ not modelled 6.9 33 Fold:Single transmembrane helix Superfamily:ISP transmembrane anchor Family:ISP transmembrane anchor 22 c3pgeA_ not modelled 6.9 12 PDB header:dna binding protein Chain: A: PDB Molecule:sumo-modified proliferating cell nuclear antigen; PDBTitle: structure of sumoylated pcna 23 d2uubc1 not modelled 6.9 31 Fold:Alpha-lytic protease prodomain-like Superfamily:Prokaryotic type KH domain (KH-domain type II) Family:Prokaryotic type KH domain (KH-domain type II) 24 d1twfc1 not modelled 6.9 29 Fold:DCoH-like Superfamily:RBP11-like subunits of RNA polymerase Family:RNA polymerase alpha subunit dimerisation domain 25 d1whya_ not modelled 6.8 22 Fold:Ferredoxin-like Superfamily:RNA-binding domain, RBD Family:Canonical RBD 26 d1ji8a_ not modelled 6.8 26 Fold:DsrC, the gamma subunit of dissimilatory sulfite reductase Superfamily:DsrC, the gamma subunit of dissimilatory sulfite reductase Family:DsrC, the gamma subunit of dissimilatory sulfite reductase 27 c2kaeA_ not modelled 6.3 25 PDB header:transcription/dna Chain: A: PDB Molecule:gata-type transcription factor; PDBTitle: data-driven model of med1:dna complex 28 c2l25A_ not modelled 5.9 17 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein; PDBTitle: np_888769.1 29 c1a3qA_ not modelled 5.7 19 PDB header:transcription/dna Chain: A: PDB Molecule:protein (nuclear factor kappa-b p52); PDBTitle: human nf-kappa-b p52 bound to dna 30 c3s2xB_ not modelled 5.7 37 PDB header:transferase Chain: B: PDB Molecule:acetyl-coa synthase subunit alpha; PDBTitle: structure of acetyl-coenzyme a synthase alpha subunit c-terminal2 domain 31 d2fug31 not modelled 5.4 16 Fold:Double psi beta-barrel Superfamily:ADC-like Family:Formate dehydrogenase/DMSO reductase, C-terminal domain 32 c2l1qA_ not modelled 5.4 58 PDB header:antimicrobial protein Chain: A: PDB Molecule:liver-expressed antimicrobial peptide 2; PDBTitle: solution structure of human liver expressed antimicrobial peptide 2 33 d2vuti1 not modelled 5.3 43 Fold:Glucocorticoid receptor-like (DNA-binding domain) Superfamily:Glucocorticoid receptor-like (DNA-binding domain) Family:Erythroid transcription factor GATA-1 34 d2ieca1 not modelled 5.3 24 Fold:MK0786-like Superfamily:MK0786-like Family:MK0786-like 35 d1gjwa1 not modelled 5.2 28 Fold:Glycosyl hydrolase domain Superfamily:Glycosyl hydrolase domain Family:alpha-Amylases, C-terminal beta-sheet domain 36 d1f2fa_ not modelled 5.2 50 Fold:SH2-like Superfamily:SH2 domain Family:SH2 domain 37 c2kvoA_ not modelled 5.0 17 PDB header:photosynthesis Chain: A: PDB Molecule:photosystem ii reaction center psb28 protein; PDBTitle: solution nmr structure of photosystem ii reaction center psb28 protein2 from synechocystis sp.(strain pcc 6803), northeast structural3 genomics consortium target sgr171 38 c2de4B_ not modelled 5.0 20 PDB header:hydrolase Chain: B: PDB Molecule:dibenzothiophene desulfurization enzyme b; PDBTitle: crystal structure of dszb c27s mutant in complex with biphenyl-2-2 sulfinic acid

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0