Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	S	P	C	E	N	D	T	P	I	N	W	K	R	N	L	I	V	A	W	L	G	C	F	L	T	G	A	A	F	S	L	V	M	P	F	L	P	L	Y	V	E	Q	L	G	V	T	G	H	S	A	L	N	M	W	S	G	I	V	F
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?																																		?				?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		S	I	T	F	L	F	S	A	I	A	S	P	F	W	G	G	L	A	D	R	K	G	R	K	L	M	L	L	R	S	A	L	G	M	G	I	V	M	V	L	M	G	L	A	Q	N	I	W	Q	F	L	I	L	R	A	L	L	G	L	L
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		G	G	F	V	P	N	A	N	A	L	I	A	T	Q	V	P	R	N	K	S	G	W	A	L	G	T	L	S	T	G	G	V	S	G	A	L	L	G	P	M	A	G	G	L	L	A	D	S	Y	G	L	R	P	V	F	F	I	T	A	S
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		V	L	I	L	C	F	F	V	T	L	F	C	I	R	E	K	F	Q	P	V	S	K	K	E	M	L	H	M	R	E	V	V	T	S	L	K	N	P	K	L	V	L	S	L	F	V	T	T	L	I	I	Q	V	A	T	G	S	I	A	P
Secondary structure
SS confidence
Disorder																?	?	?	?	?	?	?	?	?	?	?	?	?	?								?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		I	L	T	L	Y	V	R	E	L	A	G	N	V	S	N	V	A	F	I	S	G	M	I	A	S	V	P	G	V	A	A	L	L	S	A	P	R	L	G	K	L	G	D	R	I	G	P	E	K	I	L	I	T	A	L	I	F	S	V	L
Secondary structure
SS confidence
Disorder										?		?			?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		L	L	I	P	M	S	Y	V	Q	T	P	L	Q	L	G	I	L	R	F	L	L	G	A	A	D	G	A	L	L	P	A	V	Q	T	L	L	V	Y	N	S	S	N	Q	I	A	G	R	I	F	S	Y	N	Q	S	F	R	D	I	G	N
Secondary structure
SS confidence
Disorder																																								?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.
Sequence		V	T	G	P	L	M	G	A	A	I	S	A	N	Y	G	F	R	A	V	F	L	V	T	A	G	V	V	L	F	N	A	V	Y	S	W	N	S	L	R	R	R	R	I	P	Q	V	S	N
Secondary structure
SS confidence
Disorder																																								?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1pw4 chain A Region: 1 - 405 Aligned: 399 Modelled: 400 Confidence: 100.0% Identity: 13% Fold: MFS general substrate transporter Superfamily: MFS general substrate transporter Family: Glycerol-3-phosphate transporter Phyre2 PDB 1pv7 chain A Region: 12 - 402 Aligned: 386 Modelled: 391 Confidence: 100.0% Identity: 14% Fold: MFS general substrate transporter Superfamily: MFS general substrate transporter Family: LacY-like proton/sugar symporter Phyre2 PDB 2gfp chain A Region: 16 - 391 Aligned: 368 Modelled: 376 Confidence: 99.9% Identity: 15% PDB header:membrane protein Chain: A: PDB Molecule:multidrug resistance protein d; PDBTitle: structure of the multidrug transporter emrd from2 escherichia coli Phyre2 PDB 3o7p chain A Region: 14 - 400 Aligned: 380 Modelled: 387 Confidence: 99.9% Identity: 12% PDB header:transport protein Chain: A: PDB Molecule:l-fucose-proton symporter; PDBTitle: crystal structure of the e.coli fucose:proton symporter, fucp (n162a) Phyre2 PDB 2xut chain C Region: 18 - 394 Aligned: 377 Modelled: 377 Confidence: 99.9% Identity: 11% PDB header:transport protein Chain: C: PDB Molecule:proton/peptide symporter family protein; PDBTitle: crystal structure of a proton dependent oligopeptide (pot)2 family transporter. Phyre2 PDB 3mku chain A Region: 6 - 407 Aligned: 402 Modelled: 402 Confidence: 37.7% Identity: 9% PDB header:transport protein Chain: A: PDB Molecule:multi antimicrobial extrusion protein (na(+)/drug PDBTitle: structure of a cation-bound multidrug and toxin compound extrusion2 (mate) transporter Phyre2 PDB 3lrc chain C Region: 277 - 404 Aligned: 123 Modelled: 128 Confidence: 28.3% Identity: 11% PDB header:transport protein Chain: C: PDB Molecule:arginine/agmatine antiporter; PDBTitle: structure of e. coli adic (p1) Phyre2 PDB 3rko chain F Region: 375 - 406 Aligned: 32 Modelled: 32 Confidence: 22.7% Identity: 16% PDB header:oxidoreductase Chain: F: PDB Molecule:nadh-quinone oxidoreductase subunit j; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 e. coli at 3.0 angstrom resolution Phyre2 PDB 2g9p chain A Region: 73 - 86 Aligned: 14 Modelled: 14 Confidence: 20.9% Identity: 36% PDB header:antimicrobial protein Chain: A: PDB Molecule:antimicrobial peptide latarcin 2a; PDBTitle: nmr structure of a novel antimicrobial peptide, latarcin 2a,2 from spider (lachesana tarabaevi) venom Phyre2 PDB 2bbj chain B Region: 336 - 402 Aligned: 65 Modelled: 67 Confidence: 16.9% Identity: 17% PDB header:metal transport/membrane protein Chain: B: PDB Molecule:divalent cation transport-related protein; PDBTitle: crystal structure of the cora mg2+ transporter Phyre2 PDB 2k9p chain A Region: 368 - 404 Aligned: 37 Modelled: 37 Confidence: 13.6% Identity: 19% PDB header:membrane protein Chain: A: PDB Molecule:pheromone alpha factor receptor; PDBTitle: structure of tm1_tm2 in lppg micelles Phyre2 PDB 2bbn chain B Region: 10 - 23 Aligned: 14 Modelled: 14 Confidence: 9.9% Identity: 29% PDB header:calcium-binding protein Chain: B: PDB Molecule:myosin light chain kinase; PDBTitle: solution structure of a calmodulin-target peptide complex2 by multidimensional nmr Phyre2 PDB 2bbm chain B Region: 10 - 23 Aligned: 14 Modelled: 14 Confidence: 9.9% Identity: 29% PDB header:calcium-binding protein Chain: B: PDB Molecule:myosin light chain kinase; PDBTitle: solution structure of a calmodulin-target peptide complex2 by multidimensional nmr Phyre2 PDB 3bl2 chain A domain 1 Region: 10 - 39 Aligned: 30 Modelled: 30 Confidence: 9.2% Identity: 23% Fold: Toxins' membrane translocation domains Superfamily: Bcl-2 inhibitors of programmed cell death Family: Bcl-2 inhibitors of programmed cell death Phyre2 PDB 3hd6 chain A Region: 5 - 219 Aligned: 206 Modelled: 215 Confidence: 8.2% Identity: 8% PDB header:membrane protein, transport protein Chain: A: PDB Molecule:ammonium transporter rh type c; PDBTitle: crystal structure of the human rhesus glycoprotein rhcg Phyre2 PDB 2k21 chain A Region: 378 - 408 Aligned: 31 Modelled: 31 Confidence: 6.7% Identity: 13% PDB header:membrane protein Chain: A: PDB Molecule:potassium voltage-gated channel subfamily e PDBTitle: nmr structure of human kcne1 in lmpg micelles at ph 6.0 and2 40 degree c Phyre2 PDB 3b60 chain A domain 2 Region: 10 - 84 Aligned: 75 Modelled: 75 Confidence: 6.6% Identity: 17% Fold: ABC transporter transmembrane region Superfamily: ABC transporter transmembrane region Family: ABC transporter transmembrane region Phyre2 PDB 3kf9 chain D Region: 10 - 23 Aligned: 14 Modelled: 14 Confidence: 6.4% Identity: 29% PDB header:cell cycle/calcium-binding protein Chain: D: PDB Molecule:myosin light chain kinase 2, skeletal/cardiac muscle; PDBTitle: crystal structure of the sdcen/skmlck complex Phyre2 PDB 1pf4 chain A domain 2 Region: 10 - 84 Aligned: 75 Modelled: 75 Confidence: 6.4% Identity: 9% Fold: ABC transporter transmembrane region Superfamily: ABC transporter transmembrane region Family: ABC transporter transmembrane region Phyre2 PDB 3kf9 chain B Region: 10 - 23 Aligned: 14 Modelled: 14 Confidence: 6.2% Identity: 29% PDB header:cell cycle/calcium-binding protein Chain: B: PDB Molecule:myosin light chain kinase 2, skeletal/cardiac muscle; PDBTitle: crystal structure of the sdcen/skmlck complex Phyre2 1 d1pw4a_ 2 d1pv7a_ 3 c2gfpA_ 4 c3o7pA_ 5 c2xutC_ 6 c3mkuA_ 7 c3lrcC_ 8 c3rkoF_ 9 c2g9pA_ 10 c2bbjB_ 11 c2k9pA_ 12 c2bbnB_ 13 c2bbmB_ 14 d3bl2a1 15 c3hd6A_ 16 c2k21A_ 17 d3b60a2 18 c3kf9D_ 19 d1pf4a2 20 c3kf9B_ 21

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1pw4a_ 100.0 13 Fold:MFS general substrate transporter Superfamily:MFS general substrate transporter Family:Glycerol-3-phosphate transporter 2 d1pv7a_ 100.0 14 Fold:MFS general substrate transporter Superfamily:MFS general substrate transporter Family:LacY-like proton/sugar symporter 3 c2gfpA_ 99.9 15 PDB header:membrane protein Chain: A: PDB Molecule:multidrug resistance protein d; PDBTitle: structure of the multidrug transporter emrd from2 escherichia coli 4 c3o7pA_ 99.9 12 PDB header:transport protein Chain: A: PDB Molecule:l-fucose-proton symporter; PDBTitle: crystal structure of the e.coli fucose:proton symporter, fucp (n162a) 5 c2xutC_ 99.9 11 PDB header:transport protein Chain: C: PDB Molecule:proton/peptide symporter family protein; PDBTitle: crystal structure of a proton dependent oligopeptide (pot)2 family transporter. 6 c3mkuA_ 37.7 9 PDB header:transport protein Chain: A: PDB Molecule:multi antimicrobial extrusion protein (na(+)/drug PDBTitle: structure of a cation-bound multidrug and toxin compound extrusion2 (mate) transporter 7 c3lrcC_ 28.3 11 PDB header:transport protein Chain: C: PDB Molecule:arginine/agmatine antiporter; PDBTitle: structure of e. coli adic (p1) 8 c3rkoF_ 22.7 16 PDB header:oxidoreductase Chain: F: PDB Molecule:nadh-quinone oxidoreductase subunit j; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 e. coli at 3.0 angstrom resolution 9 c2g9pA_ 20.9 36 PDB header:antimicrobial protein Chain: A: PDB Molecule:antimicrobial peptide latarcin 2a; PDBTitle: nmr structure of a novel antimicrobial peptide, latarcin 2a,2 from spider (lachesana tarabaevi) venom 10 c2bbjB_ 16.9 17 PDB header:metal transport/membrane protein Chain: B: PDB Molecule:divalent cation transport-related protein; PDBTitle: crystal structure of the cora mg2+ transporter 11 c2k9pA_ 13.6 19 PDB header:membrane protein Chain: A: PDB Molecule:pheromone alpha factor receptor; PDBTitle: structure of tm1_tm2 in lppg micelles 12 c2bbnB_ 9.9 29 PDB header:calcium-binding protein Chain: B: PDB Molecule:myosin light chain kinase; PDBTitle: solution structure of a calmodulin-target peptide complex2 by multidimensional nmr 13 c2bbmB_ 9.9 29 PDB header:calcium-binding protein Chain: B: PDB Molecule:myosin light chain kinase; PDBTitle: solution structure of a calmodulin-target peptide complex2 by multidimensional nmr 14 d3bl2a1 9.2 23 Fold:Toxins' membrane translocation domains Superfamily:Bcl-2 inhibitors of programmed cell death Family:Bcl-2 inhibitors of programmed cell death 15 c3hd6A_ 8.2 8 PDB header:membrane protein, transport protein Chain: A: PDB Molecule:ammonium transporter rh type c; PDBTitle: crystal structure of the human rhesus glycoprotein rhcg 16 c2k21A_ 6.7 13 PDB header:membrane protein Chain: A: PDB Molecule:potassium voltage-gated channel subfamily e PDBTitle: nmr structure of human kcne1 in lmpg micelles at ph 6.0 and2 40 degree c 17 d3b60a2 6.6 17 Fold:ABC transporter transmembrane region Superfamily:ABC transporter transmembrane region Family:ABC transporter transmembrane region 18 c3kf9D_ 6.4 29 PDB header:cell cycle/calcium-binding protein Chain: D: PDB Molecule:myosin light chain kinase 2, skeletal/cardiac muscle; PDBTitle: crystal structure of the sdcen/skmlck complex 19 d1pf4a2 6.4 9 Fold:ABC transporter transmembrane region Superfamily:ABC transporter transmembrane region Family:ABC transporter transmembrane region 20 c3kf9B_ 6.2 29 PDB header:cell cycle/calcium-binding protein Chain: B: PDB Molecule:myosin light chain kinase 2, skeletal/cardiac muscle; PDBTitle: crystal structure of the sdcen/skmlck complex 21 c2ksfA_ not modelled 6.0 14 PDB header:transferase Chain: A: PDB Molecule:sensor protein kdpd; PDBTitle: backbone structure of the membrane domain of e. coli2 histidine kinase receptor kdpd, center for structures of3 membrane proteins (csmp) target 4312c

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0