Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	F	Q	Q	E	V	T	I	T	A	P	N	G	L	H	T	R	P	A	A	Q	F	V	K	E	A	K	G	F	T	S	E	I	T	V	T	S	N	G	K	S	A	S	A	K	S	L	F	K	L	Q	T	L	G	L	T	Q	G	T	V
Secondary structure
SS confidence
Disorder		?	?																																										?									?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.
Sequence		V	T	I	S	A	E	G	E	D	E	Q	K	A	V	E	H	L	V	K	L	M	A	E	L	E
Secondary structure
SS confidence
Disorder																								?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1cm3 chain A Region: 1 - 85 Aligned: 84 Modelled: 85 Confidence: 100.0% Identity: 98% Fold: HPr-like Superfamily: HPr-like Family: HPr-like Phyre2 PDB 1ka5 chain A Region: 1 - 83 Aligned: 83 Modelled: 83 Confidence: 100.0% Identity: 33% Fold: HPr-like Superfamily: HPr-like Family: HPr-like Phyre2 PDB 3ihs chain B Region: 1 - 82 Aligned: 82 Modelled: 81 Confidence: 100.0% Identity: 37% PDB header:transport protein Chain: B: PDB Molecule:phosphocarrier protein hpr; PDBTitle: crystal structure of a phosphocarrier protein hpr from2 bacillus anthracis str. ames Phyre2 PDB 1mo1 chain A Region: 1 - 83 Aligned: 83 Modelled: 83 Confidence: 100.0% Identity: 46% Fold: HPr-like Superfamily: HPr-like Family: HPr-like Phyre2 PDB 1ptf chain A Region: 1 - 85 Aligned: 85 Modelled: 85 Confidence: 100.0% Identity: 35% Fold: HPr-like Superfamily: HPr-like Family: HPr-like Phyre2 PDB 1qr5 chain A Region: 1 - 83 Aligned: 83 Modelled: 83 Confidence: 100.0% Identity: 35% Fold: HPr-like Superfamily: HPr-like Family: HPr-like Phyre2 PDB 3le1 chain B Region: 1 - 83 Aligned: 83 Modelled: 83 Confidence: 100.0% Identity: 43% PDB header:transferase Chain: B: PDB Molecule:phosphotransferase system, hpr-related proteins; PDBTitle: crystal structure of apohpr monomer from thermoanaerobacter2 tengcongensis Phyre2 PDB 1pch chain A Region: 3 - 85 Aligned: 83 Modelled: 83 Confidence: 100.0% Identity: 34% Fold: HPr-like Superfamily: HPr-like Family: HPr-like Phyre2 PDB 2hpr chain A Region: 3 - 83 Aligned: 81 Modelled: 81 Confidence: 100.0% Identity: 36% Fold: HPr-like Superfamily: HPr-like Family: HPr-like Phyre2 PDB 1zvv chain J domain 1 Region: 1 - 83 Aligned: 82 Modelled: 83 Confidence: 100.0% Identity: 45% Fold: HPr-like Superfamily: HPr-like Family: HPr-like Phyre2 PDB 2nzu chain L domain 1 Region: 3 - 83 Aligned: 80 Modelled: 81 Confidence: 100.0% Identity: 40% Fold: HPr-like Superfamily: HPr-like Family: HPr-like Phyre2 PDB 1vq3 chain A Region: 54 - 84 Aligned: 31 Modelled: 31 Confidence: 63.6% Identity: 32% Fold: PurS-like Superfamily: PurS-like Family: PurS subunit of FGAM synthetase Phyre2 PDB 2dgb chain A Region: 28 - 84 Aligned: 57 Modelled: 57 Confidence: 60.6% Identity: 16% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical protein purs; PDBTitle: structure of thermus thermophilus purs in the p21 form Phyre2 PDB 2jpi chain A Region: 1 - 83 Aligned: 76 Modelled: 78 Confidence: 58.0% Identity: 14% PDB header:structural genomics Chain: A: PDB Molecule:hypothetical protein; PDBTitle: chemical shift assignments of pa4090 from pseudomonas2 aeruginosa Phyre2 PDB 1gtd chain A Region: 54 - 84 Aligned: 31 Modelled: 31 Confidence: 50.4% Identity: 10% Fold: PurS-like Superfamily: PurS-like Family: PurS subunit of FGAM synthetase Phyre2 PDB 2zw2 chain B Region: 54 - 84 Aligned: 31 Modelled: 31 Confidence: 42.4% Identity: 23% PDB header:ligase Chain: B: PDB Molecule:putative uncharacterized protein sts178; PDBTitle: crystal structure of formylglycinamide ribonucleotide amidotransferase2 iii from sulfolobus tokodaii (stpurs) Phyre2 PDB 3goe chain A Region: 4 - 65 Aligned: 60 Modelled: 62 Confidence: 42.1% Identity: 13% PDB header:recombination, replication Chain: A: PDB Molecule:dna repair protein rad60; PDBTitle: molecular mimicry of sumo promotes dna repair Phyre2 PDB 2eke chain C Region: 20 - 66 Aligned: 45 Modelled: 47 Confidence: 18.4% Identity: 13% PDB header:ligase/protein binding Chain: C: PDB Molecule:ubiquitin-like protein smt3; PDBTitle: structure of a sumo-binding-motif mimic bound to smt3p-2 ubc9p: conservation of a noncovalent ubiquitin-like3 protein-e2 complex as a platform for selective4 interactions within a sumo pathway Phyre2 PDB 2etn chain A domain 2 Region: 40 - 67 Aligned: 28 Modelled: 28 Confidence: 14.9% Identity: 21% Fold: FKBP-like Superfamily: FKBP-like Family: GreA transcript cleavage factor, C-terminal domain Phyre2 PDB 2etn chain A Region: 40 - 67 Aligned: 28 Modelled: 28 Confidence: 13.4% Identity: 21% PDB header:transcription Chain: A: PDB Molecule:anti-cleavage anti-grea transcription factor PDBTitle: crystal structure of thermus aquaticus gfh1 Phyre2 1 d1cm3a_ 2 d1ka5a_ 3 c3ihsB_ 4 d1mo1a_ 5 d1ptfa_ 6 d1qr5a_ 7 c3le1B_ 8 d1pcha_ 9 d2hpra_ 10 d1zvvj1 11 d2nzul1 12 d1vq3a_ 13 c2dgbA_ 14 c2jpiA_ 15 d1gtda_ 16 c2zw2B_ 17 c3goeA_ 18 c2ekeC_ 19 d2etna2 20 c2etnA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1cm3a_ 100.0 98 Fold:HPr-like Superfamily:HPr-like Family:HPr-like 2 d1ka5a_ 100.0 33 Fold:HPr-like Superfamily:HPr-like Family:HPr-like 3 c3ihsB_ 100.0 37 PDB header:transport protein Chain: B: PDB Molecule:phosphocarrier protein hpr; PDBTitle: crystal structure of a phosphocarrier protein hpr from2 bacillus anthracis str. ames 4 d1mo1a_ 100.0 46 Fold:HPr-like Superfamily:HPr-like Family:HPr-like 5 d1ptfa_ 100.0 35 Fold:HPr-like Superfamily:HPr-like Family:HPr-like 6 d1qr5a_ 100.0 35 Fold:HPr-like Superfamily:HPr-like Family:HPr-like 7 c3le1B_ 100.0 43 PDB header:transferase Chain: B: PDB Molecule:phosphotransferase system, hpr-related proteins; PDBTitle: crystal structure of apohpr monomer from thermoanaerobacter2 tengcongensis 8 d1pcha_ 100.0 34 Fold:HPr-like Superfamily:HPr-like Family:HPr-like 9 d2hpra_ 100.0 36 Fold:HPr-like Superfamily:HPr-like Family:HPr-like 10 d1zvvj1 100.0 45 Fold:HPr-like Superfamily:HPr-like Family:HPr-like 11 d2nzul1 100.0 40 Fold:HPr-like Superfamily:HPr-like Family:HPr-like 12 d1vq3a_ 63.6 32 Fold:PurS-like Superfamily:PurS-like Family:PurS subunit of FGAM synthetase 13 c2dgbA_ 60.6 16 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical protein purs; PDBTitle: structure of thermus thermophilus purs in the p21 form 14 c2jpiA_ 58.0 14 PDB header:structural genomics Chain: A: PDB Molecule:hypothetical protein; PDBTitle: chemical shift assignments of pa4090 from pseudomonas2 aeruginosa 15 d1gtda_ 50.4 10 Fold:PurS-like Superfamily:PurS-like Family:PurS subunit of FGAM synthetase 16 c2zw2B_ 42.4 23 PDB header:ligase Chain: B: PDB Molecule:putative uncharacterized protein sts178; PDBTitle: crystal structure of formylglycinamide ribonucleotide amidotransferase2 iii from sulfolobus tokodaii (stpurs) 17 c3goeA_ 42.1 13 PDB header:recombination, replication Chain: A: PDB Molecule:dna repair protein rad60; PDBTitle: molecular mimicry of sumo promotes dna repair 18 c2ekeC_ 18.4 13 PDB header:ligase/protein binding Chain: C: PDB Molecule:ubiquitin-like protein smt3; PDBTitle: structure of a sumo-binding-motif mimic bound to smt3p-2 ubc9p: conservation of a noncovalent ubiquitin-like3 protein-e2 complex as a platform for selective4 interactions within a sumo pathway 19 d2etna2 14.9 21 Fold:FKBP-like Superfamily:FKBP-like Family:GreA transcript cleavage factor, C-terminal domain 20 c2etnA_ 13.4 21 PDB header:transcription Chain: A: PDB Molecule:anti-cleavage anti-grea transcription factor PDBTitle: crystal structure of thermus aquaticus gfh1 21 d1t4aa_ not modelled 13.2 13 Fold:PurS-like Superfamily:PurS-like Family:PurS subunit of FGAM synthetase 22 d1yema_ not modelled 12.8 21 Fold:CYTH-like phosphatases Superfamily:CYTH-like phosphatases Family:CYTH domain 23 d1srva_ not modelled 12.6 19 Fold:The "swivelling" beta/beta/alpha domain Superfamily:GroEL apical domain-like Family:GroEL-like chaperone, apical domain 24 d1r11a3 not modelled 12.3 17 Fold:MutS N-terminal domain-like Superfamily:tRNA-intron endonuclease N-terminal domain-like Family:tRNA-intron endonuclease N-terminal domain-like 25 c2e6gI_ not modelled 12.2 20 PDB header:hydrolase Chain: I: PDB Molecule:5'-nucleotidase sure; PDBTitle: crystal structure of the stationary phase survival protein sure from2 thermus thermophilus hb8 in complex with phosphate 26 c2dc4A_ not modelled 11.2 11 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:165aa long hypothetical protein; PDBTitle: structure of ph1012 protein from pyrococcus horikoshii ot3 27 d1dk7a_ not modelled 11.2 19 Fold:The "swivelling" beta/beta/alpha domain Superfamily:GroEL apical domain-like Family:GroEL-like chaperone, apical domain 28 d2vbua1 not modelled 10.9 31 Fold:Reductase/isomerase/elongation factor common domain Superfamily:Riboflavin kinase-like Family:CTP-dependent riboflavin kinase-like 29 c3k6qB_ not modelled 10.1 29 PDB header:ligand binding protein Chain: B: PDB Molecule:putative ligand binding protein; PDBTitle: crystal structure of an antitoxin part of a putative toxin/antitoxin2 system (swol_0700) from syntrophomonas wolfei subsp. wolfei at 1.80 a3 resolution 30 d2f23a2 not modelled 10.0 23 Fold:FKBP-like Superfamily:FKBP-like Family:GreA transcript cleavage factor, C-terminal domain 31 c3bmbB_ not modelled 10.0 20 PDB header:rna binding protein Chain: B: PDB Molecule:regulator of nucleoside diphosphate kinase; PDBTitle: crystal structure of a new rna polymerase interacting2 protein 32 d1we3a2 not modelled 9.5 20 Fold:The "swivelling" beta/beta/alpha domain Superfamily:GroEL apical domain-like Family:GroEL-like chaperone, apical domain 33 d1l5xa_ not modelled 9.1 17 Fold:SurE-like Superfamily:SurE-like Family:SurE-like 34 c2pn0D_ not modelled 8.7 26 PDB header:transcription Chain: D: PDB Molecule:prokaryotic transcription elongation factor PDBTitle: prokaryotic transcription elongation factor grea/greb from2 nitrosomonas europaea 35 d1euvb_ not modelled 8.6 13 Fold:beta-Grasp (ubiquitin-like) Superfamily:Ubiquitin-like Family:Ubiquitin-related 36 d1aopa2 not modelled 8.6 24 Fold:Ferredoxin-like Superfamily:Nitrite/Sulfite reductase N-terminal domain-like Family:Duplicated SiR/NiR-like domains 1 and 3 37 d1sjpa2 not modelled 8.6 23 Fold:The "swivelling" beta/beta/alpha domain Superfamily:GroEL apical domain-like Family:GroEL-like chaperone, apical domain 38 c3m6cA_ not modelled 8.1 23 PDB header:chaperone Chain: A: PDB Molecule:60 kda chaperonin 1; PDBTitle: crystal structure of mycobacterium tuberculosis groel1 apical domain 39 c2p4vA_ not modelled 8.1 15 PDB header:transcription Chain: A: PDB Molecule:transcription elongation factor greb; PDBTitle: crystal structure of the transcript cleavage factor, greb2 at 2.6a resolution 40 d1w96c1 not modelled 7.8 38 Fold:Barrel-sandwich hybrid Superfamily:Rudiment single hybrid motif Family:BC C-terminal domain-like 41 c2l66B_ not modelled 7.8 13 PDB header:transcription regulator Chain: B: PDB Molecule:transcriptional regulator, abrb family; PDBTitle: the dna-recognition fold of sso7c4 suggests a new member of spovt-abrb2 superfamily from archaea. 42 c2v4oB_ not modelled 7.6 17 PDB header:hydrolase Chain: B: PDB Molecule:multifunctional protein sur e; PDBTitle: crystal structure of salmonella typhimurium sure at 2.752 angstrom resolution in monoclinic form 43 d1grja2 not modelled 7.6 26 Fold:FKBP-like Superfamily:FKBP-like Family:GreA transcript cleavage factor, C-terminal domain 44 c2jxxA_ not modelled 7.4 14 PDB header:protein binding Chain: A: PDB Molecule:nfatc2-interacting protein; PDBTitle: nmr solution structure of ubiquitin-like domain of2 nfatc2ip. northeast structural genomics consortium target3 hr5627 45 d2fy9a1 not modelled 7.4 12 Fold:Double-split beta-barrel Superfamily:AbrB/MazE/MraZ-like Family:AbrB N-terminal domain-like 46 c1ztgD_ not modelled 7.3 7 PDB header:dna, rna binding protein/dna Chain: D: PDB Molecule:poly(rc)-binding protein 1; PDBTitle: human alpha polyc binding protein kh1 47 d1yfba1 not modelled 7.1 29 Fold:Double-split beta-barrel Superfamily:AbrB/MazE/MraZ-like Family:AbrB N-terminal domain-like 48 d1kida_ not modelled 6.9 20 Fold:The "swivelling" beta/beta/alpha domain Superfamily:GroEL apical domain-like Family:GroEL-like chaperone, apical domain 49 d1b5sa_ not modelled 6.8 33 Fold:CoA-dependent acyltransferases Superfamily:CoA-dependent acyltransferases Family:CAT-like 50 d1ulza1 not modelled 6.8 13 Fold:Barrel-sandwich hybrid Superfamily:Rudiment single hybrid motif Family:BC C-terminal domain-like 51 d2uyzb1 not modelled 6.6 11 Fold:beta-Grasp (ubiquitin-like) Superfamily:Ubiquitin-like Family:Ubiquitin-related 52 c1ibaA_ not modelled 6.5 12 PDB header:phoshphotransferase Chain: A: PDB Molecule:glucose permease; PDBTitle: glucose permease (domain iib), nmr, 11 structures 53 d2io3b1 not modelled 6.3 7 Fold:beta-Grasp (ubiquitin-like) Superfamily:Ubiquitin-like Family:Ubiquitin-related 54 c2ro5B_ not modelled 6.2 22 PDB header:transcription Chain: B: PDB Molecule:stage v sporulation protein t; PDBTitle: rdc-refined solution structure of the n-terminal dna2 recognition domain of the bacillus subtilis transition-3 state regulator spovt 55 c2ii4C_ not modelled 6.2 5 PDB header:transferase Chain: C: PDB Molecule:lipoamide acyltransferase component of branched-chain PDBTitle: crystal structure of a cubic core of the dihydrolipoamide2 acyltransferase (e2b) component in the branched-chain alpha-ketoacid3 dehydrogenase complex (bckdc), coenzyme a-bound form 56 d1dpba_ not modelled 6.2 19 Fold:CoA-dependent acyltransferases Superfamily:CoA-dependent acyltransferases Family:CAT-like 57 c2yx5A_ not modelled 6.1 23 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:upf0062 protein mj1593; PDBTitle: crystal structure of methanocaldococcus jannaschii purs, one of the2 subunits of formylglycinamide ribonucleotide amidotransferase in the3 purine biosynthetic pathway 58 d2j9ga1 not modelled 6.1 22 Fold:Barrel-sandwich hybrid Superfamily:Rudiment single hybrid motif Family:BC C-terminal domain-like 59 d1q2ha_ not modelled 6.1 50 Fold:Dimerisation interlock Superfamily:Phenylalanine zipper Family:Adapter protein APS, dimerisation domain 60 c2w1tB_ not modelled 6.0 24 PDB header:transcription Chain: B: PDB Molecule:stage v sporulation protein t; PDBTitle: crystal structure of b. subtilis spovt 61 d2bfdb2 not modelled 5.8 21 Fold:TK C-terminal domain-like Superfamily:TK C-terminal domain-like Family:Branched-chain alpha-keto acid dehydrogenase beta-subunit, C-terminal-domain 62 c3gebC_ not modelled 5.6 14 PDB header:hydrolase Chain: C: PDB Molecule:eyes absent homolog 2; PDBTitle: crystal structure of edeya2 63 c2hjwA_ not modelled 5.4 25 PDB header:ligase Chain: A: PDB Molecule:acetyl-coa carboxylase 2; PDBTitle: crystal structure of the bc domain of acc2 64 d1j9ja_ not modelled 5.3 13 Fold:SurE-like Superfamily:SurE-like Family:SurE-like 65 c2k8hA_ not modelled 5.3 13 PDB header:signaling protein Chain: A: PDB Molecule:small ubiquitin protein; PDBTitle: solution structure of sumo from trypanosoma brucei 66 d1w96a1 not modelled 5.1 38 Fold:Barrel-sandwich hybrid Superfamily:Rudiment single hybrid motif Family:BC C-terminal domain-like 67 d1oela2 not modelled 5.1 18 Fold:The "swivelling" beta/beta/alpha domain Superfamily:GroEL apical domain-like Family:GroEL-like chaperone, apical domain

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0