Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	E	T	F	N	S	L	F	M	V	S	P	L	L	L	G	V	L	F	F	V	A	M	L	A	G	F	I	D	S	I	A	G	G	G	G	L	L	T	I	P	A	L	M	A	A	G	M	S	P	A	N	A	L	A	T	N	K	L	Q
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		A	C	G	G	S	I	S	A	T	I	Y	F	I	R	R	K	V	V	S	L	S	D	Q	K	L	N	I	A	M	T	F	V	G	S	M	S	G	A	L	L	V	Q	Y	V	Q	A	D	V	L	R	Q	I	L	P	I	L	V	I	C	I
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		G	L	Y	F	L	L	M	P	K	L	G	E	E	D	R	Q	R	R	M	Y	G	L	P	F	A	L	I	A	G	G	C	V	G	F	Y	D	G	F	F	G	P	A	A	G	S	F	Y	A	L	A	F	V	T	L	C	G	F	N	L	A
Secondary structure
SS confidence
Disorder							?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		K	A	T	A	H	A	K	L	L	N	A	T	S	N	I	G	G	L	L	L	F	I	L	G	G	K	V	I	W	A	T	G	F	V	M	L	V	G	Q	F	L	G	A	R	M	G	S	R	L	V	L	S	K	G	Q	K	L	I	R	P
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.
Sequence		M	I	V	I	V	S	A	V	M	S	A	K	L	L	Y	D	S	H	G	Q	E	I	L	H	W	L	G	M	N
Secondary structure
SS confidence
Disorder																											?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 3lrc chain C Region: 54 - 134 Aligned: 80 Modelled: 81 Confidence: 14.9% Identity: 10% PDB header:transport protein Chain: C: PDB Molecule:arginine/agmatine antiporter; PDBTitle: structure of e. coli adic (p1) Phyre2 PDB 3gia chain A Region: 36 - 140 Aligned: 105 Modelled: 105 Confidence: 13.6% Identity: 10% PDB header:transport protein Chain: A: PDB Molecule:uncharacterized protein mj0609; PDBTitle: crystal structure of apct transporter Phyre2 PDB 1j4n chain A Region: 65 - 157 Aligned: 93 Modelled: 93 Confidence: 11.7% Identity: 11% Fold: Aquaporin-like Superfamily: Aquaporin-like Family: Aquaporin-like Phyre2 PDB 2j83 chain B Region: 256 - 268 Aligned: 13 Modelled: 13 Confidence: 9.1% Identity: 38% PDB header:hydrolase Chain: B: PDB Molecule:ulilysin; PDBTitle: ulilysin metalloprotease in complex with batimastat. Phyre2 PDB 3llq chain B Region: 65 - 236 Aligned: 138 Modelled: 143 Confidence: 8.9% Identity: 13% PDB header:membrane protein Chain: B: PDB Molecule:aquaporin z 2; PDBTitle: aquaporin structure from plant pathogen agrobacterium tumerfaciens Phyre2 PDB 1fx8 chain A Region: 65 - 154 Aligned: 90 Modelled: 90 Confidence: 8.7% Identity: 12% Fold: Aquaporin-like Superfamily: Aquaporin-like Family: Aquaporin-like Phyre2 PDB 2d57 chain A Region: 65 - 163 Aligned: 99 Modelled: 99 Confidence: 7.0% Identity: 17% PDB header:transport protein Chain: A: PDB Molecule:aquaporin-4; PDBTitle: double layered 2d crystal structure of aquaporin-4 (aqp4m23) at 3.2 a2 resolution by electron crystallography Phyre2 PDB 1lda chain A Region: 65 - 238 Aligned: 154 Modelled: 168 Confidence: 6.5% Identity: 18% PDB header:transport protein Chain: A: PDB Molecule:glycerol uptake facilitator protein; PDBTitle: crystal structure of the e. coli glycerol facilitator (glpf) without2 substrate glycerol Phyre2 PDB 1ymg chain A domain 1 Region: 65 - 157 Aligned: 93 Modelled: 93 Confidence: 6.5% Identity: 15% Fold: Aquaporin-like Superfamily: Aquaporin-like Family: Aquaporin-like Phyre2 PDB 1ymg chain A Region: 65 - 157 Aligned: 93 Modelled: 93 Confidence: 6.5% Identity: 15% PDB header:membrane protein Chain: A: PDB Molecule:lens fiber major intrinsic protein; PDBTitle: the channel architecture of aquaporin o at 2.2 angstrom resolution Phyre2 PDB 3klz chain E Region: 18 - 104 Aligned: 87 Modelled: 87 Confidence: 6.3% Identity: 17% PDB header:membrane protein Chain: E: PDB Molecule:putative formate transporter 1; PDBTitle: pentameric formate channel with formate bound Phyre2 PDB 1cii chain A Region: 193 - 235 Aligned: 43 Modelled: 43 Confidence: 6.2% Identity: 16% PDB header:transmembrane protein Chain: A: PDB Molecule:colicin ia; PDBTitle: colicin ia Phyre2 PDB 1u7g chain A Region: 50 - 205 Aligned: 155 Modelled: 156 Confidence: 5.9% Identity: 12% Fold: Ammonium transporter Superfamily: Ammonium transporter Family: Ammonium transporter Phyre2 PDB 3c02 chain A Region: 65 - 233 Aligned: 152 Modelled: 169 Confidence: 5.8% Identity: 16% PDB header:membrane protein Chain: A: PDB Molecule:aquaglyceroporin; PDBTitle: x-ray structure of the aquaglyceroporin from plasmodium falciparum Phyre2 PDB 3hfw chain A Region: 216 - 241 Aligned: 26 Modelled: 26 Confidence: 5.7% Identity: 8% PDB header:hydrolase Chain: A: PDB Molecule:protein adp-ribosylarginine hydrolase; PDBTitle: crystal structure of human adp-ribosylhydrolase 1 (harh1) Phyre2 PDB 1rc2 chain A Region: 65 - 201 Aligned: 137 Modelled: 137 Confidence: 5.6% Identity: 10% Fold: Aquaporin-like Superfamily: Aquaporin-like Family: Aquaporin-like Phyre2 PDB 1h6i chain A Region: 65 - 163 Aligned: 99 Modelled: 99 Confidence: 5.5% Identity: 8% Fold: Aquaporin-like Superfamily: Aquaporin-like Family: Aquaporin-like Phyre2 PDB 2ht2 chain B Region: 71 - 257 Aligned: 165 Modelled: 174 Confidence: 5.5% Identity: 11% PDB header:membrane protein Chain: B: PDB Molecule:h(+)/cl(-) exchange transporter clca; PDBTitle: structure of the escherichia coli clc chloride channel2 y445h mutant and fab complex Phyre2 PDB 3g9d chain B Region: 216 - 241 Aligned: 26 Modelled: 26 Confidence: 5.4% Identity: 19% PDB header:hydrolase Chain: B: PDB Molecule:dinitrogenase reductase activacting PDBTitle: crystal structure glycohydrolase Phyre2 PDB 2jp3 chain A Region: 111 - 142 Aligned: 32 Modelled: 32 Confidence: 5.3% Identity: 16% PDB header:transcription Chain: A: PDB Molecule:fxyd domain-containing ion transport regulator 4; PDBTitle: solution structure of the human fxyd4 (chif) protein in sds2 micelles Phyre2 1 c3lrcC_ 2 c3giaA_ 3 d1j4na_ 4 c2j83B_ 5 c3llqB_ 6 d1fx8a_ 7 c2d57A_ 8 c1ldaA_ 9 d1ymga1 10 c1ymgA_ 11 c3klzE_ 12 c1ciiA_ 13 d1u7ga_ 14 c3c02A_ 15 c3hfwA_ 16 d1rc2a_ 17 d1h6ia_ 18 c2ht2B_ 19 c3g9dB_ 20 c2jp3A_ 21

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c3lrcC_ 14.9 10 PDB header:transport protein Chain: C: PDB Molecule:arginine/agmatine antiporter; PDBTitle: structure of e. coli adic (p1) 2 c3giaA_ 13.6 10 PDB header:transport protein Chain: A: PDB Molecule:uncharacterized protein mj0609; PDBTitle: crystal structure of apct transporter 3 d1j4na_ 11.7 11 Fold:Aquaporin-like Superfamily:Aquaporin-like Family:Aquaporin-like 4 c2j83B_ 9.1 38 PDB header:hydrolase Chain: B: PDB Molecule:ulilysin; PDBTitle: ulilysin metalloprotease in complex with batimastat. 5 c3llqB_ 8.9 13 PDB header:membrane protein Chain: B: PDB Molecule:aquaporin z 2; PDBTitle: aquaporin structure from plant pathogen agrobacterium tumerfaciens 6 d1fx8a_ 8.7 12 Fold:Aquaporin-like Superfamily:Aquaporin-like Family:Aquaporin-like 7 c2d57A_ 7.0 17 PDB header:transport protein Chain: A: PDB Molecule:aquaporin-4; PDBTitle: double layered 2d crystal structure of aquaporin-4 (aqp4m23) at 3.2 a2 resolution by electron crystallography 8 c1ldaA_ 6.5 18 PDB header:transport protein Chain: A: PDB Molecule:glycerol uptake facilitator protein; PDBTitle: crystal structure of the e. coli glycerol facilitator (glpf) without2 substrate glycerol 9 d1ymga1 6.5 15 Fold:Aquaporin-like Superfamily:Aquaporin-like Family:Aquaporin-like 10 c1ymgA_ 6.5 15 PDB header:membrane protein Chain: A: PDB Molecule:lens fiber major intrinsic protein; PDBTitle: the channel architecture of aquaporin o at 2.2 angstrom resolution 11 c3klzE_ 6.3 17 PDB header:membrane protein Chain: E: PDB Molecule:putative formate transporter 1; PDBTitle: pentameric formate channel with formate bound 12 c1ciiA_ 6.2 16 PDB header:transmembrane protein Chain: A: PDB Molecule:colicin ia; PDBTitle: colicin ia 13 d1u7ga_ 5.9 12 Fold:Ammonium transporter Superfamily:Ammonium transporter Family:Ammonium transporter 14 c3c02A_ 5.8 16 PDB header:membrane protein Chain: A: PDB Molecule:aquaglyceroporin; PDBTitle: x-ray structure of the aquaglyceroporin from plasmodium falciparum 15 c3hfwA_ 5.7 8 PDB header:hydrolase Chain: A: PDB Molecule:protein adp-ribosylarginine hydrolase; PDBTitle: crystal structure of human adp-ribosylhydrolase 1 (harh1) 16 d1rc2a_ 5.6 10 Fold:Aquaporin-like Superfamily:Aquaporin-like Family:Aquaporin-like 17 d1h6ia_ 5.5 8 Fold:Aquaporin-like Superfamily:Aquaporin-like Family:Aquaporin-like 18 c2ht2B_ 5.5 11 PDB header:membrane protein Chain: B: PDB Molecule:h(+)/cl(-) exchange transporter clca; PDBTitle: structure of the escherichia coli clc chloride channel2 y445h mutant and fab complex 19 c3g9dB_ 5.4 19 PDB header:hydrolase Chain: B: PDB Molecule:dinitrogenase reductase activacting PDBTitle: crystal structure glycohydrolase 20 c2jp3A_ 5.3 16 PDB header:transcription Chain: A: PDB Molecule:fxyd domain-containing ion transport regulator 4; PDBTitle: solution structure of the human fxyd4 (chif) protein in sds2 micelles 21 d1kqfb2 not modelled 5.2 12 Fold:Single transmembrane helix Superfamily:Iron-sulfur subunit of formate dehydrogenase N, transmembrane anchor Family:Iron-sulfur subunit of formate dehydrogenase N, transmembrane anchor

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0