Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	T	E	Q	E	K	T	S	A	V	V	E	E	T	R	E	A	V	D	T	T	S	Q	P	V	A	T	E	K	K	S	K	N	N	T	A	L	I	L	S	A	V	A	I	A	I	A	L	A	A	G	I	G	L	Y	G	W	G	K	Q
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		Q	A	V	N	Q	T	A	T	S	D	A	L	A	N	Q	L	T	A	L	Q	K	A	Q	E	S	Q	K	A	E	L	E	G	I	I	K	Q	Q	A	A	Q	L	K	Q	A	N	R	Q	Q	E	T	L	A	K	Q	L	D	E	V	Q	Q
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		K	V	A	T	I	S	G	S	D	A	K	T	W	L	L	A	Q	A	D	F	L	V	K	L	A	G	R	K	L	W	S	D	Q	D	V	T	T	A	A	A	L	L	K	S	A	D	A	S	L	A	D	M	N	D	P	S	L	I	T	V
Secondary structure
SS confidence
Disorder						?	?	?	?	?																																											?		?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		R	R	A	I	T	D	D	I	A	S	L	S	A	V	S	Q	V	D	Y	D	G	I	I	L	K	L	N	Q	L	S	N	Q	V	D	N	L	R	L	A	D	N	D	S	D	G	S	P	M	D	S	D	G	E	E	L	S	S	S	I	S
Secondary structure
SS confidence
Disorder														?	?	?	?	?	?																?		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		E	W	R	I	N	L	Q	K	S	W	Q	N	F	M	D	N	F	I	T	I	R	R	R	D	D	T	A	V	P	L	L	A	P	N	Q	D	I	Y	L	R	E	N	I	R	S	R	L	L	V	A	A	Q	A	V	P	R	H	Q	E	E
Secondary structure
SS confidence
Disorder																																																											?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		T	Y	R	Q	A	L	E	N	V	S	T	W	V	R	A	Y	Y	D	T	D	D	A	T	T	K	A	F	L	D	E	V	D	Q	L	S	Q	Q	N	I	S	M	D	L	P	E	T	L	Q	S	Q	A	M	L	E	K	L	M	Q	T	R
Secondary structure
SS confidence
Disorder																																					?	?	?	?	?	?	?		?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.
Sequence		V	R	N	L	L	A	Q	P	A	A	G	T	T	E	A	K	P	A	P	A	P	Q	A	D	T	P	A	A	A	P	Q	G	E
Secondary structure
SS confidence
Disorder			?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2zbi chain B Region: 150 - 216 Aligned: 64 Modelled: 67 Confidence: 48.8% Identity: 16% PDB header:structural protein Chain: B: PDB Molecule:flagellin homolog; PDBTitle: crysatl structure of a bacterial cell-surface flagellin Phyre2 PDB 1deq chain O Region: 58 - 126 Aligned: 69 Modelled: 69 Confidence: 43.4% Identity: 10% PDB header: PDB COMPND: Phyre2 PDB 2a6q chain B domain 1 Region: 238 - 285 Aligned: 48 Modelled: 48 Confidence: 28.4% Identity: 27% Fold: YefM-like Superfamily: YefM-like Family: YefM-like Phyre2 PDB 2d4x chain A Region: 141 - 216 Aligned: 73 Modelled: 76 Confidence: 27.6% Identity: 16% PDB header:structural protein Chain: A: PDB Molecule:flagellar hook-associated protein 3; PDBTitle: crystal structure of a 26k fragment of hap3 (flgl) Phyre2 PDB 3k8w chain A Region: 135 - 216 Aligned: 79 Modelled: 82 Confidence: 26.3% Identity: 16% PDB header:structural protein Chain: A: PDB Molecule:flagellin homolog; PDBTitle: crysatl structure of a bacterial cell-surface flagellin n20c45 Phyre2 PDB 2y69 chain Q Region: 31 - 61 Aligned: 31 Modelled: 31 Confidence: 19.9% Identity: 13% PDB header:electron transport Chain: Q: PDB Molecule:cytochrome c oxidase subunit 4 isoform 1; PDBTitle: bovine heart cytochrome c oxidase re-refined with molecular2 oxygen Phyre2 PDB 3ghg chain K Region: 62 - 172 Aligned: 111 Modelled: 111 Confidence: 19.4% Identity: 5% PDB header:blood clotting Chain: K: PDB Molecule:fibrinogen beta chain; PDBTitle: crystal structure of human fibrinogen Phyre2 PDB 3g5o chain A Region: 238 - 286 Aligned: 49 Modelled: 49 Confidence: 18.7% Identity: 20% PDB header:toxin/antitoxin Chain: A: PDB Molecule:uncharacterized protein rv2865; PDBTitle: the crystal structure of the toxin-antitoxin complex relbe2 (rv2865-2 2866) from mycobacterium tuberculosis Phyre2 PDB 1v54 chain D Region: 31 - 61 Aligned: 31 Modelled: 31 Confidence: 18.5% Identity: 13% Fold: Single transmembrane helix Superfamily: Mitochondrial cytochrome c oxidase subunit IV Family: Mitochondrial cytochrome c oxidase subunit IV Phyre2 PDB 2a6q chain A domain 1 Region: 238 - 286 Aligned: 49 Modelled: 49 Confidence: 17.0% Identity: 29% Fold: YefM-like Superfamily: YefM-like Family: YefM-like Phyre2 PDB 1gk4 chain A Region: 68 - 162 Aligned: 76 Modelled: 76 Confidence: 16.7% Identity: 17% PDB header:vimentin Chain: A: PDB Molecule:vimentin; PDBTitle: human vimentin coil 2b fragment (cys2) Phyre2 PDB 1io1 chain A Region: 150 - 216 Aligned: 64 Modelled: 67 Confidence: 16.6% Identity: 22% Fold: Phase 1 flagellin Superfamily: Phase 1 flagellin Family: Phase 1 flagellin Phyre2 PDB 3k8v chain B Region: 135 - 216 Aligned: 79 Modelled: 82 Confidence: 15.6% Identity: 16% PDB header:structural protein Chain: B: PDB Molecule:flagellin homolog; PDBTitle: crysatl structure of a bacterial cell-surface flagellin n20c20 Phyre2 PDB 1ei3 chain E Region: 59 - 175 Aligned: 117 Modelled: 117 Confidence: 15.4% Identity: 8% PDB header: PDB COMPND: Phyre2 PDB 3j00 chain Z Region: 35 - 60 Aligned: 26 Modelled: 26 Confidence: 15.1% Identity: 4% PDB header:ribosome/ribosomal protein Chain: Z: PDB Molecule:cell division protein ftsq; PDBTitle: structure of the ribosome-secye complex in the membrane environment Phyre2 PDB 1ud0 chain A Region: 298 - 336 Aligned: 36 Modelled: 39 Confidence: 13.9% Identity: 11% Fold: immunoglobulin/albumin-binding domain-like Superfamily: Heat shock protein 70kD (HSP70), C-terminal subdomain Family: Heat shock protein 70kD (HSP70), C-terminal subdomain Phyre2 PDB 3ibp chain A Region: 101 - 218 Aligned: 112 Modelled: 118 Confidence: 13.5% Identity: 21% PDB header:cell cycle Chain: A: PDB Molecule:chromosome partition protein mukb; PDBTitle: the crystal structure of the dimerization domain of escherichia coli2 structural maintenance of chromosomes protein mukb Phyre2 PDB 3pe0 chain B Region: 69 - 227 Aligned: 152 Modelled: 159 Confidence: 12.9% Identity: 12% PDB header:structural protein Chain: B: PDB Molecule:plectin; PDBTitle: structure of the central region of the plakin domain of plectin Phyre2 PDB 3fap chain B Region: 300 - 323 Aligned: 24 Modelled: 24 Confidence: 11.7% Identity: 21% Fold: Four-helical up-and-down bundle Superfamily: FKBP12-rapamycin-binding domain of FKBP-rapamycin-associated protein (FRAP) Family: FKBP12-rapamycin-binding domain of FKBP-rapamycin-associated protein (FRAP) Phyre2 PDB 3lof chain F Region: 298 - 336 Aligned: 36 Modelled: 39 Confidence: 11.4% Identity: 17% PDB header:chaperone Chain: F: PDB Molecule:heat shock 70 kda protein 1; PDBTitle: c-terminal domain of human heat shock 70kda protein 1b. Phyre2 1 c2zbiB_ 2 c1deqO_ 3 d2a6qb1 4 c2d4xA_ 5 c3k8wA_ 6 c2y69Q_ 7 c3ghgK_ 8 c3g5oA_ 9 d1v54d_ 10 d2a6qa1 11 c1gk4A_ 12 d1io1a_ 13 c3k8vB_ 14 c1ei3E_ 15 c3j00Z_ 16 d1ud0a_ 17 c3ibpA_ 18 c3pe0B_ 19 d3fapb_ 20 c3lofF_ 21 22 23 24 25 26 27 28

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c2zbiB_ 48.8 16 PDB header:structural protein Chain: B: PDB Molecule:flagellin homolog; PDBTitle: crysatl structure of a bacterial cell-surface flagellin 2 c1deqO_ 43.4 10 PDB header: PDB COMPND: 3 d2a6qb1 28.4 27 Fold:YefM-like Superfamily:YefM-like Family:YefM-like 4 c2d4xA_ 27.6 16 PDB header:structural protein Chain: A: PDB Molecule:flagellar hook-associated protein 3; PDBTitle: crystal structure of a 26k fragment of hap3 (flgl) 5 c3k8wA_ 26.3 16 PDB header:structural protein Chain: A: PDB Molecule:flagellin homolog; PDBTitle: crysatl structure of a bacterial cell-surface flagellin n20c45 6 c2y69Q_ 19.9 13 PDB header:electron transport Chain: Q: PDB Molecule:cytochrome c oxidase subunit 4 isoform 1; PDBTitle: bovine heart cytochrome c oxidase re-refined with molecular2 oxygen 7 c3ghgK_ 19.4 5 PDB header:blood clotting Chain: K: PDB Molecule:fibrinogen beta chain; PDBTitle: crystal structure of human fibrinogen 8 c3g5oA_ 18.7 20 PDB header:toxin/antitoxin Chain: A: PDB Molecule:uncharacterized protein rv2865; PDBTitle: the crystal structure of the toxin-antitoxin complex relbe2 (rv2865-2 2866) from mycobacterium tuberculosis 9 d1v54d_ 18.5 13 Fold:Single transmembrane helix Superfamily:Mitochondrial cytochrome c oxidase subunit IV Family:Mitochondrial cytochrome c oxidase subunit IV 10 d2a6qa1 17.0 29 Fold:YefM-like Superfamily:YefM-like Family:YefM-like 11 c1gk4A_ 16.7 17 PDB header:vimentin Chain: A: PDB Molecule:vimentin; PDBTitle: human vimentin coil 2b fragment (cys2) 12 d1io1a_ 16.6 22 Fold:Phase 1 flagellin Superfamily:Phase 1 flagellin Family:Phase 1 flagellin 13 c3k8vB_ 15.6 16 PDB header:structural protein Chain: B: PDB Molecule:flagellin homolog; PDBTitle: crysatl structure of a bacterial cell-surface flagellin n20c20 14 c1ei3E_ 15.4 8 PDB header: PDB COMPND: 15 c3j00Z_ 15.1 4 PDB header:ribosome/ribosomal protein Chain: Z: PDB Molecule:cell division protein ftsq; PDBTitle: structure of the ribosome-secye complex in the membrane environment 16 d1ud0a_ 13.9 11 Fold:immunoglobulin/albumin-binding domain-like Superfamily:Heat shock protein 70kD (HSP70), C-terminal subdomain Family:Heat shock protein 70kD (HSP70), C-terminal subdomain 17 c3ibpA_ 13.5 21 PDB header:cell cycle Chain: A: PDB Molecule:chromosome partition protein mukb; PDBTitle: the crystal structure of the dimerization domain of escherichia coli2 structural maintenance of chromosomes protein mukb 18 c3pe0B_ 12.9 12 PDB header:structural protein Chain: B: PDB Molecule:plectin; PDBTitle: structure of the central region of the plakin domain of plectin 19 d3fapb_ 11.7 21 Fold:Four-helical up-and-down bundle Superfamily:FKBP12-rapamycin-binding domain of FKBP-rapamycin-associated protein (FRAP) Family:FKBP12-rapamycin-binding domain of FKBP-rapamycin-associated protein (FRAP) 20 c3lofF_ 11.4 17 PDB header:chaperone Chain: F: PDB Molecule:heat shock 70 kda protein 1; PDBTitle: c-terminal domain of human heat shock 70kda protein 1b. 21 c1ud0B_ not modelled 11.0 11 PDB header:chaperone Chain: B: PDB Molecule:70 kda heat-shock-like protein; PDBTitle: crystal structure of the c-terminal 10-kda subdomain of hsc70 22 c3mkxC_ not modelled 7.2 13 PDB header:antiviral protein Chain: C: PDB Molecule:bone marrow stromal antigen 2; PDBTitle: crystal structure of bst2/tetherin 23 d2e74a1 not modelled 6.5 15 Fold:Heme-binding four-helical bundle Superfamily:Transmembrane di-heme cytochromes Family:Cytochrome b of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase) 24 d1q90b_ not modelled 6.4 10 Fold:Heme-binding four-helical bundle Superfamily:Transmembrane di-heme cytochromes Family:Cytochrome b of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase) 25 c1x8yA_ not modelled 6.3 14 PDB header:structural protein Chain: A: PDB Molecule:lamin a/c; PDBTitle: human lamin coil 2b 26 c2rpaA_ not modelled 6.1 10 PDB header:hydrolase Chain: A: PDB Molecule:katanin p60 atpase-containing subunit a1; PDBTitle: the solution structure of n-terminal domain of microtubule severing2 enzyme 27 c2npuA_ not modelled 6.1 21 PDB header:transferase Chain: A: PDB Molecule:fkbp12-rapamycin complex-associated protein; PDBTitle: the solution structure of the rapamycin-binding domain of2 mtor (frb) 28 c3e1rB_ not modelled 5.9 23 PDB header:cell cycle/transport protein Chain: B: PDB Molecule:centrosomal protein of 55 kda; PDBTitle: midbody targeting of the escrt machinery by a non-canonical2 coiled-coil in cep55

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0