Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	L	T	K	K	K	W	A	L	F	S	L	L	T	L	C	G	G	T	I	Y	K	L	P	S	L	K	D	A	F	Y	I	P	M	Q	E	Y	F	H	L	T	N	G	Q	I	G	N	A	M	S	V	N	S	F	V	T	T	V	G	F
Secondary structure
SS confidence
Disorder		?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		F	L	S	I	Y	F	A	D	K	L	P	R	R	Y	T	M	S	F	S	L	I	A	T	G	L	L	G	V	Y	L	T	T	M	P	G	Y	W	G	I	L	F	V	W	A	L	F	G	V	T	C	D	M	M	N	W	P	V	L	L	K
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		S	V	S	R	L	G	N	S	E	Q	Q	G	R	L	F	G	F	F	E	T	G	R	G	I	V	D	T	V	V	A	F	S	A	L	A	V	F	T	W	F	G	S	G	L	L	G	F	K	A	G	I	W	F	Y	S	L	I	V	I	A
Secondary structure
SS confidence
Disorder																																													?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		V	G	I	I	I	F	F	V	L	N	D	K	E	E	A	P	S	V	E	V	K	K	E	D	G	A	S	K	N	T	S	M	T	S	V	L	K	D	K	T	I	W	L	I	A	F	N	V	F	F	V	Y	A	V	Y	C	G	L	T	F
Secondary structure
SS confidence
Disorder														?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		F	I	P	F	L	K	N	I	Y	L	L	P	V	A	L	V	G	A	Y	G	I	I	N	Q	Y	C	L	K	M	I	G	G	P	I	G	G	M	I	S	D	K	I	L	K	S	P	S	K	Y	L	C	Y	T	F	I	I	S	T	A	A
Secondary structure
SS confidence
Disorder											?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		L	V	L	L	I	M	L	P	H	E	S	M	P	V	Y	L	G	M	A	C	T	L	G	F	G	A	I	V	F	T	Q	R	A	V	F	F	A	P	I	G	E	A	K	I	A	E	N	K	T	G	A	A	M	A	L	G	S	F	I	G
Secondary structure
SS confidence
Disorder																																											?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		Y	A	P	A	M	F	C	F	S	L	Y	G	Y	I	L	D	L	N	P	G	I	I	G	Y	K	I	V	F	G	I	M	A	C	F	A	F	S	G	A	V	V	S	V	M	L	V	K	R	I	S	Q	R	K	K	E	M	L	A	A	E
Secondary structure
SS confidence
Disorder																																																?	?	?							?	?	?	?	?
Disorder confidence
		.
Sequence		A
Secondary structure
SS confidence
Disorder		?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1pw4 chain A Region: 3 - 419 Aligned: 407 Modelled: 413 Confidence: 100.0% Identity: 14% Fold: MFS general substrate transporter Superfamily: MFS general substrate transporter Family: Glycerol-3-phosphate transporter Phyre2 PDB 1pv7 chain A Region: 2 - 421 Aligned: 413 Modelled: 420 Confidence: 100.0% Identity: 9% Fold: MFS general substrate transporter Superfamily: MFS general substrate transporter Family: LacY-like proton/sugar symporter Phyre2 PDB 3o7p chain A Region: 5 - 404 Aligned: 383 Modelled: 397 Confidence: 100.0% Identity: 11% PDB header:transport protein Chain: A: PDB Molecule:l-fucose-proton symporter; PDBTitle: crystal structure of the e.coli fucose:proton symporter, fucp (n162a) Phyre2 PDB 2gfp chain A Region: 10 - 394 Aligned: 368 Modelled: 385 Confidence: 100.0% Identity: 10% PDB header:membrane protein Chain: A: PDB Molecule:multidrug resistance protein d; PDBTitle: structure of the multidrug transporter emrd from2 escherichia coli Phyre2 PDB 2xut chain C Region: 11 - 404 Aligned: 392 Modelled: 394 Confidence: 99.9% Identity: 6% PDB header:transport protein Chain: C: PDB Molecule:proton/peptide symporter family protein; PDBTitle: crystal structure of a proton dependent oligopeptide (pot)2 family transporter. Phyre2 PDB 3mku chain A Region: 4 - 421 Aligned: 418 Modelled: 418 Confidence: 89.7% Identity: 8% PDB header:transport protein Chain: A: PDB Molecule:multi antimicrobial extrusion protein (na(+)/drug PDBTitle: structure of a cation-bound multidrug and toxin compound extrusion2 (mate) transporter Phyre2 PDB 3qnq chain D Region: 368 - 420 Aligned: 53 Modelled: 53 Confidence: 64.7% Identity: 19% PDB header:membrane protein, transport protein Chain: D: PDB Molecule:pts system, cellobiose-specific iic component; PDBTitle: crystal structure of the transporter chbc, the iic component from the2 n,n'-diacetylchitobiose-specific phosphotransferase system Phyre2 PDB 2rdd chain B Region: 388 - 418 Aligned: 31 Modelled: 31 Confidence: 35.7% Identity: 16% PDB header:membrane protein/transport protein Chain: B: PDB Molecule:upf0092 membrane protein yajc; PDBTitle: x-ray crystal structure of acrb in complex with a novel2 transmembrane helix. Phyre2 PDB 3rko chain N Region: 4 - 412 Aligned: 382 Modelled: 390 Confidence: 32.6% Identity: 9% PDB header:oxidoreductase Chain: N: PDB Molecule:nadh-quinone oxidoreductase subunit n; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 e. coli at 3.0 angstrom resolution Phyre2 PDB 2g9p chain A Region: 62 - 75 Aligned: 14 Modelled: 14 Confidence: 10.6% Identity: 21% PDB header:antimicrobial protein Chain: A: PDB Molecule:antimicrobial peptide latarcin 2a; PDBTitle: nmr structure of a novel antimicrobial peptide, latarcin 2a,2 from spider (lachesana tarabaevi) venom Phyre2 PDB 2k21 chain A Region: 388 - 416 Aligned: 29 Modelled: 29 Confidence: 8.2% Identity: 17% PDB header:membrane protein Chain: A: PDB Molecule:potassium voltage-gated channel subfamily e PDBTitle: nmr structure of human kcne1 in lmpg micelles at ph 6.0 and2 40 degree c Phyre2 PDB 2klu chain A Region: 389 - 419 Aligned: 31 Modelled: 31 Confidence: 6.8% Identity: 19% PDB header:immune system, membrane protein Chain: A: PDB Molecule:t-cell surface glycoprotein cd4; PDBTitle: nmr structure of the transmembrane and cytoplasmic domains2 of human cd4 Phyre2 PDB 3pro chain C domain 1 Region: 30 - 71 Aligned: 42 Modelled: 42 Confidence: 6.6% Identity: 12% Fold: Alpha-lytic protease prodomain-like Superfamily: Alpha-lytic protease prodomain Family: Alpha-lytic protease prodomain Phyre2 PDB 3rko chain F Region: 285 - 413 Aligned: 129 Modelled: 129 Confidence: 6.3% Identity: 13% PDB header:oxidoreductase Chain: F: PDB Molecule:nadh-quinone oxidoreductase subunit j; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 e. coli at 3.0 angstrom resolution Phyre2 PDB 1fft chain B domain 2 Region: 384 - 416 Aligned: 33 Modelled: 33 Confidence: 6.1% Identity: 15% Fold: Transmembrane helix hairpin Superfamily: Cytochrome c oxidase subunit II-like, transmembrane region Family: Cytochrome c oxidase subunit II-like, transmembrane region Phyre2 PDB 3mk7 chain K Region: 317 - 421 Aligned: 104 Modelled: 105 Confidence: 6.0% Identity: 14% PDB header:oxidoreductase Chain: K: PDB Molecule:cytochrome c oxidase, cbb3-type, subunit n; PDBTitle: the structure of cbb3 cytochrome oxidase Phyre2 PDB 2knc chain A Region: 384 - 421 Aligned: 38 Modelled: 38 Confidence: 6.0% Identity: 13% PDB header:cell adhesion Chain: A: PDB Molecule:integrin alpha-iib; PDBTitle: platelet integrin alfaiib-beta3 transmembrane-cytoplasmic2 heterocomplex Phyre2 PDB 2nr9 chain A domain 1 Region: 393 - 415 Aligned: 23 Modelled: 23 Confidence: 5.9% Identity: 4% Fold: Rhomboid-like Superfamily: Rhomboid-like Family: Rhomboid-like Phyre2 PDB 2oar chain A domain 1 Region: 352 - 421 Aligned: 70 Modelled: 70 Confidence: 5.2% Identity: 11% Fold: Gated mechanosensitive channel Superfamily: Gated mechanosensitive channel Family: Gated mechanosensitive channel Phyre2 1 d1pw4a_ 2 d1pv7a_ 3 c3o7pA_ 4 c2gfpA_ 5 c2xutC_ 6 c3mkuA_ 7 c3qnqD_ 8 c2rddB_ 9 c3rkoN_ 10 c2g9pA_ 11 c2k21A_ 12 c2kluA_ 13 d3proc1 14 c3rkoF_ 15 d1fftb2 16 c3mk7K_ 17 c2kncA_ 18 d2nr9a1 19 d2oara1

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1pw4a_ 100.0 14 Fold:MFS general substrate transporter Superfamily:MFS general substrate transporter Family:Glycerol-3-phosphate transporter 2 d1pv7a_ 100.0 9 Fold:MFS general substrate transporter Superfamily:MFS general substrate transporter Family:LacY-like proton/sugar symporter 3 c3o7pA_ 100.0 11 PDB header:transport protein Chain: A: PDB Molecule:l-fucose-proton symporter; PDBTitle: crystal structure of the e.coli fucose:proton symporter, fucp (n162a) 4 c2gfpA_ 100.0 10 PDB header:membrane protein Chain: A: PDB Molecule:multidrug resistance protein d; PDBTitle: structure of the multidrug transporter emrd from2 escherichia coli 5 c2xutC_ 99.9 6 PDB header:transport protein Chain: C: PDB Molecule:proton/peptide symporter family protein; PDBTitle: crystal structure of a proton dependent oligopeptide (pot)2 family transporter. 6 c3mkuA_ 89.7 8 PDB header:transport protein Chain: A: PDB Molecule:multi antimicrobial extrusion protein (na(+)/drug PDBTitle: structure of a cation-bound multidrug and toxin compound extrusion2 (mate) transporter 7 c3qnqD_ 64.7 19 PDB header:membrane protein, transport protein Chain: D: PDB Molecule:pts system, cellobiose-specific iic component; PDBTitle: crystal structure of the transporter chbc, the iic component from the2 n,n'-diacetylchitobiose-specific phosphotransferase system 8 c2rddB_ 35.7 16 PDB header:membrane protein/transport protein Chain: B: PDB Molecule:upf0092 membrane protein yajc; PDBTitle: x-ray crystal structure of acrb in complex with a novel2 transmembrane helix. 9 c3rkoN_ 32.6 9 PDB header:oxidoreductase Chain: N: PDB Molecule:nadh-quinone oxidoreductase subunit n; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 e. coli at 3.0 angstrom resolution 10 c2g9pA_ 10.6 21 PDB header:antimicrobial protein Chain: A: PDB Molecule:antimicrobial peptide latarcin 2a; PDBTitle: nmr structure of a novel antimicrobial peptide, latarcin 2a,2 from spider (lachesana tarabaevi) venom 11 c2k21A_ 8.2 17 PDB header:membrane protein Chain: A: PDB Molecule:potassium voltage-gated channel subfamily e PDBTitle: nmr structure of human kcne1 in lmpg micelles at ph 6.0 and2 40 degree c 12 c2kluA_ 6.8 19 PDB header:immune system, membrane protein Chain: A: PDB Molecule:t-cell surface glycoprotein cd4; PDBTitle: nmr structure of the transmembrane and cytoplasmic domains2 of human cd4 13 d3proc1 6.6 12 Fold:Alpha-lytic protease prodomain-like Superfamily:Alpha-lytic protease prodomain Family:Alpha-lytic protease prodomain 14 c3rkoF_ 6.3 13 PDB header:oxidoreductase Chain: F: PDB Molecule:nadh-quinone oxidoreductase subunit j; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 e. coli at 3.0 angstrom resolution 15 d1fftb2 6.1 15 Fold:Transmembrane helix hairpin Superfamily:Cytochrome c oxidase subunit II-like, transmembrane region Family:Cytochrome c oxidase subunit II-like, transmembrane region 16 c3mk7K_ 6.0 14 PDB header:oxidoreductase Chain: K: PDB Molecule:cytochrome c oxidase, cbb3-type, subunit n; PDBTitle: the structure of cbb3 cytochrome oxidase 17 c2kncA_ 6.0 13 PDB header:cell adhesion Chain: A: PDB Molecule:integrin alpha-iib; PDBTitle: platelet integrin alfaiib-beta3 transmembrane-cytoplasmic2 heterocomplex 18 d2nr9a1 5.9 4 Fold:Rhomboid-like Superfamily:Rhomboid-like Family:Rhomboid-like 19 d2oara1 5.2 11 Fold:Gated mechanosensitive channel Superfamily:Gated mechanosensitive channel Family:Gated mechanosensitive channel

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0