Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	F	K	S	F	F	P	K	P	G	T	F	F	L	S	A	F	V	W	A	L	I	A	V	I	F	W	Q	A	G	G	G	D	W	V	A	R	I	T	G	A	S	G	Q	I	P	I	S	A	A	R	F	W	S	L	D	F	L	I	F
Secondary structure
SS confidence
Disorder		?	?	?	?	?																																				?	?	?	?	?										?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		Y	A	Y	Y	I	V	C	V	G	L	F	A	L	F	W	F	I	Y	S	P	H	R	W	Q	Y	W	S	I	L	G	T	A	L	I	I	F	V	T	W	F	L	V	E	V	G	V	A	V	N	A	W	Y	A	P	F	Y	D	L	I	Q
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		T	A	L	S	S	P	H	K	V	T	I	E	Q	F	Y	R	E	V	G	V	F	L	G	I	A	L	I	A	V	V	I	S	V	L	N	N	F	F	V	S	H	Y	V	F	R	W	R	T	A	M	N	E	Y	Y	M	A	N	W	Q	Q
Secondary structure
SS confidence
Disorder				?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		L	R	H	I	E	G	A	A	Q	R	V	Q	E	D	T	M	R	F	A	S	T	L	E	N	M	G	V	S	F	I	N	A	I	M	T	L	I	A	F	L	P	V	L	V	T	L	S	A	H	V	P	E	L	P	I	I	G	H	I	P
Secondary structure
SS confidence
Disorder		?	?	?	?		?																																												?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		Y	G	L	V	I	A	A	I	V	W	S	L	M	G	T	G	L	L	A	V	V	G	I	K	L	P	G	L	E	F	K	N	Q	R	V	E	A	A	Y	R	K	E	L	V	Y	G	E	D	D	A	T	R	A	T	P	P	T	V	R	E
Secondary structure
SS confidence
Disorder																								?					?	?							?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		L	F	S	A	V	R	K	N	Y	F	R	L	Y	F	H	Y	M	Y	F	N	I	A	R	I	L	Y	L	Q	V	D	N	V	F	G	L	F	L	L	F	P	S	I	V	A	G	T	I	T	L	G	L	M	T	Q	I	T	N	V	F	G
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.
Sequence		Q	V	R	G	A	F	Q	Y	L	I	N	S	W	T	T	L	V	E	L	M	S	I	Y	K	R	L	R	S	F	E	H	E	L	D	G	D	K	I	Q	E	V	T	H	T	L	S
Secondary structure
SS confidence
Disorder																																				?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 3b60 chain A domain 2 Region: 69 - 396 Aligned: 311 Modelled: 328 Confidence: 99.9% Identity: 11% Fold: ABC transporter transmembrane region Superfamily: ABC transporter transmembrane region Family: ABC transporter transmembrane region Phyre2 PDB 2yl4 chain A Region: 67 - 402 Aligned: 322 Modelled: 331 Confidence: 99.9% Identity: 11% PDB header:membrane protein Chain: A: PDB Molecule:atp-binding cassette sub-family b member 10, PDBTitle: structure of the human mitochondrial abc transporter, abcb10 Phyre2 PDB 2hyd chain A domain 2 Region: 70 - 395 Aligned: 315 Modelled: 326 Confidence: 99.9% Identity: 10% Fold: ABC transporter transmembrane region Superfamily: ABC transporter transmembrane region Family: ABC transporter transmembrane region Phyre2 PDB 2hyd chain B Region: 70 - 406 Aligned: 326 Modelled: 337 Confidence: 99.9% Identity: 10% PDB header:transport protein Chain: B: PDB Molecule:abc transporter homolog; PDBTitle: multidrug abc transporter sav1866 Phyre2 PDB 3g5u chain B Region: 68 - 404 Aligned: 326 Modelled: 337 Confidence: 99.9% Identity: 8% PDB header:membrane protein Chain: B: PDB Molecule:multidrug resistance protein 1a; PDBTitle: structure of p-glycoprotein reveals a molecular basis for2 poly-specific drug binding Phyre2 PDB 3b5w chain E Region: 71 - 400 Aligned: 305 Modelled: 311 Confidence: 99.8% Identity: 13% PDB header:membrane protein Chain: E: PDB Molecule:lipid a export atp-binding/permease protein msba; PDBTitle: crystal structure of eschericia coli msba Phyre2 PDB 1pf4 chain A domain 2 Region: 69 - 388 Aligned: 268 Modelled: 293 Confidence: 99.6% Identity: 13% Fold: ABC transporter transmembrane region Superfamily: ABC transporter transmembrane region Family: ABC transporter transmembrane region Phyre2 PDB 3b5x chain B Region: 69 - 406 Aligned: 321 Modelled: 338 Confidence: 99.6% Identity: 11% PDB header:membrane protein Chain: B: PDB Molecule:lipid a export atp-binding/permease protein msba; PDBTitle: crystal structure of msba from vibrio cholerae Phyre2 PDB 1ou5 chain A domain 1 Region: 180 - 198 Aligned: 19 Modelled: 19 Confidence: 63.8% Identity: 42% Fold: Poly A polymerase C-terminal region-like Superfamily: Poly A polymerase C-terminal region-like Family: Poly A polymerase C-terminal region-like Phyre2 PDB 1miw chain A domain 1 Region: 181 - 198 Aligned: 18 Modelled: 17 Confidence: 21.2% Identity: 33% Fold: Poly A polymerase C-terminal region-like Superfamily: Poly A polymerase C-terminal region-like Family: Poly A polymerase C-terminal region-like Phyre2 PDB 1n2a chain A domain 1 Region: 342 - 399 Aligned: 56 Modelled: 58 Confidence: 19.4% Identity: 9% Fold: GST C-terminal domain-like Superfamily: GST C-terminal domain-like Family: Glutathione S-transferase (GST), C-terminal domain Phyre2 PDB 3geh chain A Region: 337 - 405 Aligned: 69 Modelled: 69 Confidence: 18.3% Identity: 12% PDB header:hydrolase Chain: A: PDB Molecule:trna modification gtpase mnme; PDBTitle: crystal structure of mnme from nostoc in complex with gdp, folinic2 acid and zn Phyre2 PDB 1aw9 chain A domain 1 Region: 277 - 400 Aligned: 103 Modelled: 103 Confidence: 18.2% Identity: 11% Fold: GST C-terminal domain-like Superfamily: GST C-terminal domain-like Family: Glutathione S-transferase (GST), C-terminal domain Phyre2 PDB 2gsq chain A domain 1 Region: 286 - 400 Aligned: 114 Modelled: 115 Confidence: 17.1% Identity: 11% Fold: GST C-terminal domain-like Superfamily: GST C-terminal domain-like Family: Glutathione S-transferase (GST), C-terminal domain Phyre2 PDB 1m0u chain B Region: 342 - 401 Aligned: 60 Modelled: 60 Confidence: 17.1% Identity: 12% PDB header:transferase Chain: B: PDB Molecule:gst2 gene product; PDBTitle: crystal structure of the drosophila glutathione s-2 transferase-2 in complex with glutathione Phyre2 PDB 3a0h chain K Region: 9 - 20 Aligned: 12 Modelled: 12 Confidence: 16.0% Identity: 50% PDB header:electron transport Chain: K: PDB Molecule:photosystem ii reaction center protein k; PDBTitle: crystal structure of i-substituted photosystem ii complex Phyre2 PDB 2gst chain A domain 1 Region: 286 - 397 Aligned: 111 Modelled: 112 Confidence: 15.5% Identity: 14% Fold: GST C-terminal domain-like Superfamily: GST C-terminal domain-like Family: Glutathione S-transferase (GST), C-terminal domain Phyre2 PDB 1hio chain B Region: 186 - 212 Aligned: 27 Modelled: 27 Confidence: 14.3% Identity: 11% Fold: Histone-fold Superfamily: Histone-fold Family: Nucleosome core histones Phyre2 PDB 1vhe chain A Region: 376 - 404 Aligned: 29 Modelled: 29 Confidence: 13.7% Identity: 17% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:aminopeptidase/glucanase homolog; PDBTitle: crystal structure of a aminopeptidase/glucanase homolog Phyre2 PDB 2uz8 chain A Region: 342 - 401 Aligned: 60 Modelled: 60 Confidence: 12.8% Identity: 7% PDB header:rna-binding protein Chain: A: PDB Molecule:eukaryotic translation elongation factor 1 PDBTitle: the crystal structure of p18, human translation elongation2 factor 1 epsilon 1 Phyre2 1 d3b60a2 2 c2yl4A_ 3 d2hyda2 4 c2hydB_ 5 c3g5uB_ 6 c3b5wE_ 7 d1pf4a2 8 c3b5xB_ 9 d1ou5a1 10 d1miwa1 11 d1n2aa1 12 c3gehA_ 13 d1aw9a1 14 d2gsqa1 15 c1m0uB_ 16 c3a0hk_ 17 d2gsta1 18 d1hiob_ 19 c1vheA_ 20 c2uz8A_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d3b60a2 99.9 11 Fold:ABC transporter transmembrane region Superfamily:ABC transporter transmembrane region Family:ABC transporter transmembrane region 2 c2yl4A_ 99.9 11 PDB header:membrane protein Chain: A: PDB Molecule:atp-binding cassette sub-family b member 10, PDBTitle: structure of the human mitochondrial abc transporter, abcb10 3 d2hyda2 99.9 10 Fold:ABC transporter transmembrane region Superfamily:ABC transporter transmembrane region Family:ABC transporter transmembrane region 4 c2hydB_ 99.9 10 PDB header:transport protein Chain: B: PDB Molecule:abc transporter homolog; PDBTitle: multidrug abc transporter sav1866 5 c3g5uB_ 99.9 8 PDB header:membrane protein Chain: B: PDB Molecule:multidrug resistance protein 1a; PDBTitle: structure of p-glycoprotein reveals a molecular basis for2 poly-specific drug binding 6 c3b5wE_ 99.8 13 PDB header:membrane protein Chain: E: PDB Molecule:lipid a export atp-binding/permease protein msba; PDBTitle: crystal structure of eschericia coli msba 7 d1pf4a2 99.6 13 Fold:ABC transporter transmembrane region Superfamily:ABC transporter transmembrane region Family:ABC transporter transmembrane region 8 c3b5xB_ 99.6 11 PDB header:membrane protein Chain: B: PDB Molecule:lipid a export atp-binding/permease protein msba; PDBTitle: crystal structure of msba from vibrio cholerae 9 d1ou5a1 63.8 42 Fold:Poly A polymerase C-terminal region-like Superfamily:Poly A polymerase C-terminal region-like Family:Poly A polymerase C-terminal region-like 10 d1miwa1 21.2 33 Fold:Poly A polymerase C-terminal region-like Superfamily:Poly A polymerase C-terminal region-like Family:Poly A polymerase C-terminal region-like 11 d1n2aa1 19.4 9 Fold:GST C-terminal domain-like Superfamily:GST C-terminal domain-like Family:Glutathione S-transferase (GST), C-terminal domain 12 c3gehA_ 18.3 12 PDB header:hydrolase Chain: A: PDB Molecule:trna modification gtpase mnme; PDBTitle: crystal structure of mnme from nostoc in complex with gdp, folinic2 acid and zn 13 d1aw9a1 18.2 11 Fold:GST C-terminal domain-like Superfamily:GST C-terminal domain-like Family:Glutathione S-transferase (GST), C-terminal domain 14 d2gsqa1 17.1 11 Fold:GST C-terminal domain-like Superfamily:GST C-terminal domain-like Family:Glutathione S-transferase (GST), C-terminal domain 15 c1m0uB_ 17.1 12 PDB header:transferase Chain: B: PDB Molecule:gst2 gene product; PDBTitle: crystal structure of the drosophila glutathione s-2 transferase-2 in complex with glutathione 16 c3a0hk_ 16.0 50 PDB header:electron transport Chain: K: PDB Molecule:photosystem ii reaction center protein k; PDBTitle: crystal structure of i-substituted photosystem ii complex 17 d2gsta1 15.5 14 Fold:GST C-terminal domain-like Superfamily:GST C-terminal domain-like Family:Glutathione S-transferase (GST), C-terminal domain 18 d1hiob_ 14.3 11 Fold:Histone-fold Superfamily:Histone-fold Family:Nucleosome core histones 19 c1vheA_ 13.7 17 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:aminopeptidase/glucanase homolog; PDBTitle: crystal structure of a aminopeptidase/glucanase homolog 20 c2uz8A_ 12.8 7 PDB header:rna-binding protein Chain: A: PDB Molecule:eukaryotic translation elongation factor 1 PDBTitle: the crystal structure of p18, human translation elongation2 factor 1 epsilon 1 21 d1p3mh_ not modelled 12.8 11 Fold:Histone-fold Superfamily:Histone-fold Family:Nucleosome core histones 22 c1aw9A_ not modelled 12.2 7 PDB header:transferase Chain: A: PDB Molecule:glutathione s-transferase iii; PDBTitle: structure of glutathione s-transferase iii in apo form 23 d1tw9a1 not modelled 12.2 9 Fold:GST C-terminal domain-like Superfamily:GST C-terminal domain-like Family:Glutathione S-transferase (GST), C-terminal domain 24 d1iwpa_ not modelled 11.7 26 Fold:TIM beta/alpha-barrel Superfamily:Cobalamin (vitamin B12)-dependent enzymes Family:Diol dehydratase, alpha subunit 25 c2hsmA_ not modelled 10.8 8 PDB header:ligase/rna binding protein Chain: A: PDB Molecule:glutamyl-trna synthetase, cytoplasmic; PDBTitle: structural basis of yeast aminoacyl-trna synthetase complex2 formation revealed by crystal structures of two binary sub-3 complexes 26 c1z23A_ not modelled 10.5 26 PDB header:cell adhesion Chain: A: PDB Molecule:crk-associated substrate; PDBTitle: the serine-rich domain from crk-associated substrate2 (p130cas) 27 c2l81A_ not modelled 10.1 21 PDB header:cell adhesion Chain: A: PDB Molecule:enhancer of filamentation 1; PDBTitle: solution nmr structure of the serine-rich domain of hef1 (enhancer of2 filamentation 1) from homo sapiens, northeast structural genomics3 consortium target hr5554a 28 c3a0bK_ not modelled 10.1 50 PDB header:electron transport Chain: K: PDB Molecule:photosystem ii reaction center protein k; PDBTitle: crystal structure of br-substituted photosystem ii complex 29 c3a0bk_ not modelled 10.1 50 PDB header:electron transport Chain: K: PDB Molecule:photosystem ii reaction center protein k; PDBTitle: crystal structure of br-substituted photosystem ii complex 30 d1eexa_ not modelled 9.7 19 Fold:TIM beta/alpha-barrel Superfamily:Cobalamin (vitamin B12)-dependent enzymes Family:Diol dehydratase, alpha subunit 31 d1b4pa1 not modelled 9.7 12 Fold:GST C-terminal domain-like Superfamily:GST C-terminal domain-like Family:Glutathione S-transferase (GST), C-terminal domain 32 d1wspa1 not modelled 8.6 36 Fold:beta-Grasp (ubiquitin-like) Superfamily:Ubiquitin-like Family:DIX domain 33 d1id3d_ not modelled 8.5 11 Fold:Histone-fold Superfamily:Histone-fold Family:Nucleosome core histones 34 c2qx2A_ not modelled 7.4 3 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:sex pheromone staph-cam373; PDBTitle: structure of the c-terminal domain of sex pheromone staph-cam3732 precursor from staphylococcus aureus 35 c1byeA_ not modelled 7.0 7 PDB header:transferase Chain: A: PDB Molecule:protein (glutathione s-transferase); PDBTitle: glutathione s-transferase i from mais in complex with2 atrazine glutathione conjugate 36 c2yruA_ not modelled 6.6 19 PDB header:apoptosis Chain: A: PDB Molecule:steroid receptor rna activator 1; PDBTitle: solution structure of mouse steroid receptor rna activator2 1 (sra1) protein 37 d1pd3a_ not modelled 6.4 18 Fold:ROP-like Superfamily:Nonstructural protein ns2, Nep, M1-binding domain Family:Nonstructural protein ns2, Nep, M1-binding domain 38 d1tzyb_ not modelled 6.1 11 Fold:Histone-fold Superfamily:Histone-fold Family:Nucleosome core histones 39 c3lszA_ not modelled 5.7 10 PDB header:transferase Chain: A: PDB Molecule:glutathione s-transferase; PDBTitle: crystal structure of glutathione s-transferase from2 rhodobacter sphaeroides 40 d1b8xa1 not modelled 5.4 13 Fold:GST C-terminal domain-like Superfamily:GST C-terminal domain-like Family:Glutathione S-transferase (GST), C-terminal domain 41 d1tu7a1 not modelled 5.4 9 Fold:GST C-terminal domain-like Superfamily:GST C-terminal domain-like Family:Glutathione S-transferase (GST), C-terminal domain 42 c2akfB_ not modelled 5.3 18 PDB header:protein binding Chain: B: PDB Molecule:coronin-1a; PDBTitle: crystal structure of the coiled-coil domain of coronin 1 43 c2akfA_ not modelled 5.3 18 PDB header:protein binding Chain: A: PDB Molecule:coronin-1a; PDBTitle: crystal structure of the coiled-coil domain of coronin 1 44 c2akfC_ not modelled 5.3 18 PDB header:protein binding Chain: C: PDB Molecule:coronin-1a; PDBTitle: crystal structure of the coiled-coil domain of coronin 1 45 d2axtk1 not modelled 5.3 50 Fold:Single transmembrane helix Superfamily:Photosystem II reaction center protein K, PsbK Family:PsbK-like 46 c2fq8A_ not modelled 5.2 36 PDB header:unknown function Chain: A: PDB Molecule:2f; PDBTitle: nmr structure of 2f associated with lipid disc 47 c2fq5A_ not modelled 5.2 36 PDB header:unknown function Chain: A: PDB Molecule:peptide 2f; PDBTitle: nmr structure of 2f associated with lipid disc 48 c1zvaA_ not modelled 5.2 14 PDB header:viral protein Chain: A: PDB Molecule:e2 glycoprotein; PDBTitle: a structure-based mechanism of sars virus membrane fusion 49 c1ou5A_ not modelled 5.1 54 PDB header:translation, transferase Chain: A: PDB Molecule:trna cca-adding enzyme; PDBTitle: crystal structure of human cca-adding enzyme 50 d1gnwa1 not modelled 5.0 16 Fold:GST C-terminal domain-like Superfamily:GST C-terminal domain-like Family:Glutathione S-transferase (GST), C-terminal domain

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0