Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	N	A	I	I	S	P	D	Y	Y	Y	V	L	T	V	A	G	Q	S	N	A	M	A	Y	G	E	G	L	P	L	P	D	R	E	D	A	P	H	P	R	I	K	Q	L	A	R	F	A	H	T	H	P	G	G	P	P	C	H	F	N
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?															?		?	?	?	?	?	?		?	?	?	?	?	?	?									?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		D	I	I	P	L	T	H	C	P	H	D	V	Q	D	M	Q	G	Y	H	H	P	L	A	T	N	H	Q	T	Q	Y	G	T	V	G	Q	A	L	H	I	A	R	K	L	L	P	F	I	P	D	N	A	G	I	L	I	V	P	C	C	R
Secondary structure
SS confidence
Disorder					?				?	?	?	?	?		?	?	?	?							?	?		?	?	?																															?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		G	G	S	A	F	T	A	G	S	E	G	T	Y	S	E	R	H	G	A	S	H	D	A	C	R	W	G	T	D	T	P	L	Y	Q	D	L	V	S	R	T	R	A	A	L	A	K	N	P	Q	N	K	F	L	G	A	C	W	M	Q	G
Secondary structure
SS confidence
Disorder								?	?	?	?					?			?	?	?	?	?	?		?	?	?	?	?																?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		E	F	D	L	M	T	S	D	Y	A	S	H	P	Q	H	F	N	H	M	V	E	A	F	R	R	D	L	K	Q	Y	H	S	Q	L	N	N	I	T	D	A	P	W	F	C	G	D	T	T	W	Y	W	K	E	N	F	P	H	S	Y	E
Secondary structure
SS confidence
Disorder					?	?	?	?	?																						?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		A	I	Y	G	N	Y	Q	N	N	V	L	A	N	I	I	F	V	D	F	Q	Q	Q	G	E	R	G	L	T	N	A	P	D	E	D	P	D	D	L	S	T	G	Y	Y	G	S	A	Y	R	S	P	E	N	W	T	T	A	L	R	S	S
Secondary structure
SS confidence
Disorder			?		?							?	?	?	?	?		?	?	?		?	?	?	?	?	?	?		?	?	?	?	?	?	?		?	?			?			?					?			?	?	?	?		?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.
Sequence		H	F	S	T	A	A	R	R	G	I	I	S	D	R	F	V	E	A	I	L	Q	F	W	R	E	R
Secondary structure
SS confidence
Disorder																									?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 3pt5 chain A Region: 4 - 326 Aligned: 320 Modelled: 323 Confidence: 100.0% Identity: 97% PDB header:hydrolase Chain: A: PDB Molecule:nans (yjhs), a 9-o-acetyl n-acetylneuraminic acid esterase; PDBTitle: crystal structure of nans Phyre2 PDB 1zmb chain A domain 1 Region: 11 - 321 Aligned: 220 Modelled: 231 Confidence: 100.0% Identity: 22% Fold: Flavodoxin-like Superfamily: SGNH hydrolase Family: Putative acetylxylan esterase-like Phyre2 PDB 2apj chain A domain 1 Region: 6 - 321 Aligned: 226 Modelled: 243 Confidence: 100.0% Identity: 25% Fold: Flavodoxin-like Superfamily: SGNH hydrolase Family: Putative acetylxylan esterase-like Phyre2 PDB 1jrl chain A Region: 13 - 324 Aligned: 174 Modelled: 200 Confidence: 97.4% Identity: 18% Fold: Flavodoxin-like Superfamily: SGNH hydrolase Family: TAP-like Phyre2 PDB 2o14 chain A Region: 83 - 322 Aligned: 182 Modelled: 196 Confidence: 97.0% Identity: 10% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical protein yxim; PDBTitle: x-ray crystal structure of protein yxim_bacsu from bacillus2 subtilis. northeast structural genomics consortium target3 sr595 Phyre2 PDB 3hp4 chain A Region: 88 - 325 Aligned: 169 Modelled: 193 Confidence: 96.3% Identity: 15% PDB header:hydrolase Chain: A: PDB Molecule:gdsl-esterase; PDBTitle: crystal structure of psychrotrophic esterase esta from2 pseudoalteromonas sp. 643a inhibited by monoethylphosphonate Phyre2 PDB 3mil chain A Region: 9 - 325 Aligned: 192 Modelled: 197 Confidence: 95.7% Identity: 17% PDB header:hydrolase Chain: A: PDB Molecule:isoamyl acetate-hydrolyzing esterase; PDBTitle: crystal structure of isoamyl acetate-hydrolyzing esterase from2 saccharomyces cerevisiae Phyre2 PDB 1yzf chain A domain 1 Region: 13 - 326 Aligned: 189 Modelled: 206 Confidence: 95.5% Identity: 14% Fold: Flavodoxin-like Superfamily: SGNH hydrolase Family: TAP-like Phyre2 PDB 3p94 chain A Region: 147 - 324 Aligned: 148 Modelled: 157 Confidence: 95.4% Identity: 11% PDB header:hydrolase Chain: A: PDB Molecule:gdsl-like lipase; PDBTitle: crystal structure of a gdsl-like lipase (bdi_0976) from2 parabacteroides distasonis atcc 8503 at 1.93 a resolution Phyre2 PDB 1es9 chain A Region: 118 - 323 Aligned: 170 Modelled: 185 Confidence: 95.3% Identity: 11% Fold: Flavodoxin-like Superfamily: SGNH hydrolase Family: Acetylhydrolase Phyre2 PDB 2waa chain A Region: 175 - 324 Aligned: 108 Modelled: 119 Confidence: 95.1% Identity: 12% PDB header:hydrolase Chain: A: PDB Molecule:xylan esterase, putative, axe2c; PDBTitle: structure of a family two carbohydrate esterase from2 cellvibrio japonicus Phyre2 PDB 1fxw chain F Region: 118 - 323 Aligned: 171 Modelled: 185 Confidence: 94.9% Identity: 13% Fold: Flavodoxin-like Superfamily: SGNH hydrolase Family: Acetylhydrolase Phyre2 PDB 3bzw chain A Region: 115 - 322 Aligned: 168 Modelled: 184 Confidence: 94.8% Identity: 12% PDB header:hydrolase Chain: A: PDB Molecule:putative lipase; PDBTitle: crystal structure of a putative lipase from bacteroides2 thetaiotaomicron Phyre2 PDB 3bzw chain A domain 1 Region: 115 - 322 Aligned: 168 Modelled: 184 Confidence: 94.8% Identity: 12% Fold: Flavodoxin-like Superfamily: SGNH hydrolase Family: BT2961-like Phyre2 PDB 2vpt chain A Region: 131 - 324 Aligned: 155 Modelled: 169 Confidence: 94.2% Identity: 12% PDB header:hydrolase Chain: A: PDB Molecule:lipolytic enzyme; PDBTitle: clostridium thermocellum family 3 carbohydrate esterase Phyre2 PDB 2wao chain A Region: 175 - 324 Aligned: 114 Modelled: 125 Confidence: 93.4% Identity: 17% PDB header:hydrolase Chain: A: PDB Molecule:endoglucanase e; PDBTitle: structure of a family two carbohydrate esterase from2 clostridium thermocellum in complex with cellohexaose Phyre2 PDB 2q0q chain C Region: 13 - 323 Aligned: 212 Modelled: 217 Confidence: 93.2% Identity: 10% PDB header:hydrolase Chain: C: PDB Molecule:aryl esterase; PDBTitle: structure of the native m. smegmatis aryl esterase Phyre2 PDB 2o14 chain A domain 2 Region: 13 - 322 Aligned: 188 Modelled: 203 Confidence: 91.8% Identity: 18% Fold: Flavodoxin-like Superfamily: SGNH hydrolase Family: YxiM C-terminal domain-like Phyre2 PDB 1k7c chain A Region: 192 - 321 Aligned: 100 Modelled: 114 Confidence: 86.2% Identity: 12% Fold: Flavodoxin-like Superfamily: SGNH hydrolase Family: Rhamnogalacturonan acetylesterase Phyre2 PDB 3rjt chain A Region: 99 - 319 Aligned: 160 Modelled: 176 Confidence: 82.4% Identity: 16% PDB header:hydrolase Chain: A: PDB Molecule:lipolytic protein g-d-s-l family; PDBTitle: crystal structure of lipolytic protein g-d-s-l family from2 alicyclobacillus acidocaldarius subsp. acidocaldarius dsm 446 Phyre2 1 c3pt5A_ 2 d1zmba1 3 d2apja1 4 d1jrla_ 5 c2o14A_ 6 c3hp4A_ 7 c3milA_ 8 d1yzfa1 9 c3p94A_ 10 d1es9a_ 11 c2waaA_ 12 d1fxwf_ 13 c3bzwA_ 14 d3bzwa1 15 c2vptA_ 16 c2waoA_ 17 c2q0qC_ 18 d2o14a2 19 d1k7ca_ 20 c3rjtA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c3pt5A_ 100.0 97 PDB header:hydrolase Chain: A: PDB Molecule:nans (yjhs), a 9-o-acetyl n-acetylneuraminic acid esterase; PDBTitle: crystal structure of nans 2 d1zmba1 100.0 22 Fold:Flavodoxin-like Superfamily:SGNH hydrolase Family:Putative acetylxylan esterase-like 3 d2apja1 100.0 25 Fold:Flavodoxin-like Superfamily:SGNH hydrolase Family:Putative acetylxylan esterase-like 4 d1jrla_ 97.4 18 Fold:Flavodoxin-like Superfamily:SGNH hydrolase Family:TAP-like 5 c2o14A_ 97.0 10 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical protein yxim; PDBTitle: x-ray crystal structure of protein yxim_bacsu from bacillus2 subtilis. northeast structural genomics consortium target3 sr595 6 c3hp4A_ 96.3 15 PDB header:hydrolase Chain: A: PDB Molecule:gdsl-esterase; PDBTitle: crystal structure of psychrotrophic esterase esta from2 pseudoalteromonas sp. 643a inhibited by monoethylphosphonate 7 c3milA_ 95.7 17 PDB header:hydrolase Chain: A: PDB Molecule:isoamyl acetate-hydrolyzing esterase; PDBTitle: crystal structure of isoamyl acetate-hydrolyzing esterase from2 saccharomyces cerevisiae 8 d1yzfa1 95.5 14 Fold:Flavodoxin-like Superfamily:SGNH hydrolase Family:TAP-like 9 c3p94A_ 95.4 11 PDB header:hydrolase Chain: A: PDB Molecule:gdsl-like lipase; PDBTitle: crystal structure of a gdsl-like lipase (bdi_0976) from2 parabacteroides distasonis atcc 8503 at 1.93 a resolution 10 d1es9a_ 95.3 11 Fold:Flavodoxin-like Superfamily:SGNH hydrolase Family:Acetylhydrolase 11 c2waaA_ 95.1 12 PDB header:hydrolase Chain: A: PDB Molecule:xylan esterase, putative, axe2c; PDBTitle: structure of a family two carbohydrate esterase from2 cellvibrio japonicus 12 d1fxwf_ 94.9 13 Fold:Flavodoxin-like Superfamily:SGNH hydrolase Family:Acetylhydrolase 13 c3bzwA_ 94.8 12 PDB header:hydrolase Chain: A: PDB Molecule:putative lipase; PDBTitle: crystal structure of a putative lipase from bacteroides2 thetaiotaomicron 14 d3bzwa1 94.8 12 Fold:Flavodoxin-like Superfamily:SGNH hydrolase Family:BT2961-like 15 c2vptA_ 94.2 12 PDB header:hydrolase Chain: A: PDB Molecule:lipolytic enzyme; PDBTitle: clostridium thermocellum family 3 carbohydrate esterase 16 c2waoA_ 93.4 17 PDB header:hydrolase Chain: A: PDB Molecule:endoglucanase e; PDBTitle: structure of a family two carbohydrate esterase from2 clostridium thermocellum in complex with cellohexaose 17 c2q0qC_ 93.2 10 PDB header:hydrolase Chain: C: PDB Molecule:aryl esterase; PDBTitle: structure of the native m. smegmatis aryl esterase 18 d2o14a2 91.8 18 Fold:Flavodoxin-like Superfamily:SGNH hydrolase Family:YxiM C-terminal domain-like 19 d1k7ca_ 86.2 12 Fold:Flavodoxin-like Superfamily:SGNH hydrolase Family:Rhamnogalacturonan acetylesterase 20 c3rjtA_ 82.4 16 PDB header:hydrolase Chain: A: PDB Molecule:lipolytic protein g-d-s-l family; PDBTitle: crystal structure of lipolytic protein g-d-s-l family from2 alicyclobacillus acidocaldarius subsp. acidocaldarius dsm 446 21 d3dc7a1 not modelled 69.8 8 Fold:Flavodoxin-like Superfamily:SGNH hydrolase Family:BT2961-like 22 c3dciB_ not modelled 40.8 9 PDB header:hydrolase Chain: B: PDB Molecule:arylesterase; PDBTitle: the structure of a putative arylesterase from agrobacterium2 tumefaciens str. c58 23 d1vjga_ not modelled 34.6 10 Fold:Flavodoxin-like Superfamily:SGNH hydrolase Family:Hypothetical protein alr1529 24 c3dc7B_ not modelled 27.2 8 PDB header:structural genomics, unknown function Chain: B: PDB Molecule:putative uncharacterized protein lp_3323; PDBTitle: crystal structure of the protein q88sr8 from lactobacillus plantarum.2 northeast structural genomics consortium target lpr109. 25 c3d3qB_ not modelled 26.9 15 PDB header:transferase Chain: B: PDB Molecule:trna delta(2)-isopentenylpyrophosphate PDBTitle: crystal structure of trna delta(2)-isopentenylpyrophosphate2 transferase (se0981) from staphylococcus epidermidis.3 northeast structural genomics consortium target ser100 26 d1a0da_ not modelled 23.8 12 Fold:TIM beta/alpha-barrel Superfamily:Xylose isomerase-like Family:Xylose isomerase 27 d1aq0a_ not modelled 21.0 18 Fold:TIM beta/alpha-barrel Superfamily:(Trans)glycosidases Family:beta-glycanases 28 d2cyga1 not modelled 20.9 15 Fold:TIM beta/alpha-barrel Superfamily:(Trans)glycosidases Family:beta-glycanases 29 c2r2yA_ not modelled 20.8 20 PDB header:protein binding Chain: A: PDB Molecule:protein adrm1; PDBTitle: crystal structure of the proteasomal rpn13 pru-domain 30 c2z59A_ not modelled 20.8 20 PDB header:protein transport Chain: A: PDB Molecule:protein adrm1; PDBTitle: complex structures of mouse rpn13 (22-130aa) and ubiquitin 31 c3f55A_ not modelled 20.2 12 PDB header:hydrolase, allergen Chain: A: PDB Molecule:beta-1,3-glucanase; PDBTitle: crystal structure of the native endo beta-1,3-glucanase (hev b 2), a2 major allergen from hevea brasiliensis (space group p41) 32 d2hsja1 not modelled 19.1 12 Fold:Flavodoxin-like Superfamily:SGNH hydrolase Family:Acetylhydrolase 33 d1ghsa_ not modelled 15.4 18 Fold:TIM beta/alpha-barrel Superfamily:(Trans)glycosidases Family:beta-glycanases 34 c3eplA_ not modelled 15.0 18 PDB header:transferase/rna Chain: A: PDB Molecule:trna isopentenyltransferase; PDBTitle: crystallographic snapshots of eukaryotic2 dimethylallyltransferase acting on trna: insight into trna3 recognition and reaction mechanism 35 c2y6eF_ not modelled 13.3 6 PDB header:hydrolase Chain: F: PDB Molecule:ubiquitin carboxyl-terminal hydrolase 4; PDBTitle: ubiquitin specific protease 4 is inhibited by its ubiquitin-2 like domain 36 d1a0ea_ not modelled 12.6 12 Fold:TIM beta/alpha-barrel Superfamily:Xylose isomerase-like Family:Xylose isomerase 37 c3exaD_ not modelled 12.5 15 PDB header:transferase Chain: D: PDB Molecule:trna delta(2)-isopentenylpyrophosphate PDBTitle: crystal structure of the full-length trna2 isopentenylpyrophosphate transferase (bh2366) from3 bacillus halodurans, northeast structural genomics4 consortium target bhr41. 38 c3fozB_ not modelled 11.6 18 PDB header:transferase/rna Chain: B: PDB Molecule:trna delta(2)-isopentenylpyrophosphate transferase; PDBTitle: structure of e. coli isopentenyl-trna transferase in complex with e.2 coli trna(phe) 39 d1bxda_ not modelled 10.8 16 Fold:ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase Superfamily:ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase Family:Histidine kinase 40 d1a0ca_ not modelled 10.2 14 Fold:TIM beta/alpha-barrel Superfamily:Xylose isomerase-like Family:Xylose isomerase 41 c1y80A_ not modelled 8.9 8 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:predicted cobalamin binding protein; PDBTitle: structure of a corrinoid (factor iiim)-binding protein from2 moorella thermoacetica 42 d1t1ra2 not modelled 8.2 21 Fold:NAD(P)-binding Rossmann-fold domains Superfamily:NAD(P)-binding Rossmann-fold domains Family:Glyceraldehyde-3-phosphate dehydrogenase-like, N-terminal domain 43 c2ibiA_ not modelled 7.8 6 PDB header:hydrolase Chain: A: PDB Molecule:ubiquitin carboxyl-terminal hydrolase 2; PDBTitle: covalent ubiquitin-usp2 complex 44 d1tdha3 not modelled 7.2 50 Fold:Glucocorticoid receptor-like (DNA-binding domain) Superfamily:Glucocorticoid receptor-like (DNA-binding domain) Family:C-terminal, Zn-finger domain of MutM-like DNA repair proteins 45 c2yh6C_ not modelled 6.5 6 PDB header:lipid binding protein Chain: C: PDB Molecule:lipoprotein 34; PDBTitle: structure of the n-terminal domain of bamc from e. coli 46 c3jsbA_ not modelled 6.4 25 PDB header:rna binding protein Chain: A: PDB Molecule:rna-directed rna polymerase; PDBTitle: crystal structure of the n-terminal domain of the lymphocytic2 choriomeningitis virus l protein 47 d2jrxa1 not modelled 6.1 27 Fold:YejL-like Superfamily:YejL-like Family:YejL-like 48 d2oeba1 not modelled 6.0 33 Fold:ATPD N-terminal domain-like Superfamily:AF1862-like Family:Cas Cmr5-like 49 d2jpqa1 not modelled 6.0 23 Fold:YejL-like Superfamily:YejL-like Family:YejL-like 50 c3aysA_ not modelled 5.5 9 PDB header:hydrolase Chain: A: PDB Molecule:endoglucanase; PDBTitle: gh5 endoglucanase from a ruminal fungus in complex with cellotriose 51 c3bjxB_ not modelled 5.3 14 PDB header:hydrolase Chain: B: PDB Molecule:halocarboxylic acid dehalogenase dehi; PDBTitle: structure of a group i haloacid dehalogenase from2 pseudomonas putida strain pp3 52 c3ieiD_ not modelled 5.3 7 PDB header:transferase Chain: D: PDB Molecule:leucine carboxyl methyltransferase 1; PDBTitle: crystal structure of human leucine carboxylmethyltransferase-1 in2 complex with s-adenosyl homocysteine 53 d1wioa2 not modelled 5.2 29 Fold:Immunoglobulin-like beta-sandwich Superfamily:Immunoglobulin Family:V set domains (antibody variable domain-like) 54 c2yihA_ not modelled 5.1 7 PDB header:hydrolase Chain: A: PDB Molecule:cel44c; PDBTitle: structure of a paenibacillus polymyxa xyloglucanase from gh2 family 44 with xyloglucan 55 d1onka_ not modelled 5.1 15 Fold:Ribosome inactivating proteins (RIP) Superfamily:Ribosome inactivating proteins (RIP) Family:Plant cytotoxins

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0