Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	N	M	M	R	I	F	Y	I	G	L	S	G	V	G	M	M	F	S	S	M	A	S	G	N	D	A	G	G	L	Q	S	P	A	C	G	V	V	C	D	P	Y	I	C	V	N	S	D	G	I	S	P	E	L	T	R	K	Y	L	G
Secondary structure
SS confidence
Disorder		?	?																				?	?	?	?	?	?	?	?	?	?			?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		E	K	A	A	E	N	L	Q	S	L	Q	G	Y	D	P	S	E	F	T	F	A	N	G	V	F	C	D	V	K	E	K	L	C	R	D	D	R	Y	F	G	V	D	G	K	R	S	G	K	I	N	Q	T	T	T	K	M	L	F	M	C
Secondary structure
SS confidence
Disorder										?	?	?	?	?		?	?																						?		?	?	?	?	?	?	?	?	?	?	?									?	?
Disorder confidence
		.	.
Sequence		R	E
Secondary structure
SS confidence
Disorder		?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1ig4 chain A Region: 51 - 91 Aligned: 31 Modelled: 41 Confidence: 50.9% Identity: 32% Fold: DNA-binding domain Superfamily: DNA-binding domain Family: Methyl-CpG-binding domain, MBD Phyre2 PDB 2a2b chain A Region: 79 - 97 Aligned: 17 Modelled: 19 Confidence: 49.6% Identity: 41% PDB header:antibiotic Chain: A: PDB Molecule:bacteriocin curvacin a; PDBTitle: curvacin a Phyre2 PDB 2q3e chain H Region: 1 - 21 Aligned: 21 Modelled: 21 Confidence: 16.6% Identity: 29% PDB header:oxidoreductase Chain: H: PDB Molecule:udp-glucose 6-dehydrogenase; PDBTitle: structure of human udp-glucose dehydrogenase complexed with nadh and2 udp-glucose Phyre2 PDB 1flc chain A domain 1 Region: 39 - 49 Aligned: 11 Modelled: 11 Confidence: 11.7% Identity: 45% Fold: Viral protein domain Superfamily: Viral protein domain Family: Hemagglutinin domain of haemagglutinin-esterase-fusion glycoprotein HEF1 Phyre2 PDB 1ua7 chain A domain 1 Region: 79 - 88 Aligned: 10 Modelled: 10 Confidence: 11.0% Identity: 10% Fold: Glycosyl hydrolase domain Superfamily: Glycosyl hydrolase domain Family: alpha-Amylases, C-terminal beta-sheet domain Phyre2 PDB 3ebk chain A Region: 78 - 113 Aligned: 32 Modelled: 36 Confidence: 9.9% Identity: 22% PDB header:allergen Chain: A: PDB Molecule:allergen bla g 4; PDBTitle: crystal structure of major allergens, bla g 4 from2 cockroaches Phyre2 PDB 2yqp chain A Region: 27 - 44 Aligned: 18 Modelled: 18 Confidence: 8.5% Identity: 44% PDB header:gene regulation, hydrolase Chain: A: PDB Molecule:probable atp-dependent rna helicase ddx59; PDBTitle: solution structure of the zf-hit domain in dead (asp-glu-2 ala-asp) box polypeptide 59 Phyre2 PDB 1q90 chain R Region: 1 - 24 Aligned: 24 Modelled: 24 Confidence: 8.5% Identity: 33% Fold: Single transmembrane helix Superfamily: ISP transmembrane anchor Family: ISP transmembrane anchor Phyre2 PDB 1q90 chain R Region: 1 - 24 Aligned: 24 Modelled: 24 Confidence: 8.5% Identity: 33% PDB header:photosynthesis Chain: R: PDB Molecule:cytochrome b6-f complex iron-sulfur subunit; PDBTitle: structure of the cytochrome b6f (plastohydroquinone : plastocyanin2 oxidoreductase) from chlamydomonas reinhardtii Phyre2 PDB 3pln chain A Region: 2 - 21 Aligned: 20 Modelled: 20 Confidence: 8.1% Identity: 25% PDB header:oxidoreductase Chain: A: PDB Molecule:udp-glucose 6-dehydrogenase; PDBTitle: crystal structure of klebsiella pneumoniae udp-glucose 6-dehydrogenase2 complexed with udp-glucose Phyre2 PDB 1qki chain A domain 2 Region: 58 - 85 Aligned: 20 Modelled: 28 Confidence: 7.6% Identity: 45% Fold: FwdE/GAPDH domain-like Superfamily: Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain Family: Glucose 6-phosphate dehydrogenase-like Phyre2 PDB 3ggp chain A Region: 1 - 41 Aligned: 36 Modelled: 38 Confidence: 7.5% Identity: 19% PDB header:oxidoreductase Chain: A: PDB Molecule:prephenate dehydrogenase; PDBTitle: crystal structure of prephenate dehydrogenase from a. aeolicus in2 complex with hydroxyphenyl propionate and nad+ Phyre2 PDB 1hx0 chain A domain 1 Region: 79 - 88 Aligned: 10 Modelled: 10 Confidence: 7.1% Identity: 40% Fold: Glycosyl hydrolase domain Superfamily: Glycosyl hydrolase domain Family: alpha-Amylases, C-terminal beta-sheet domain Phyre2 PDB 3prj chain B Region: 1 - 21 Aligned: 21 Modelled: 21 Confidence: 7.0% Identity: 29% PDB header:oxidoreductase Chain: B: PDB Molecule:udp-glucose 6-dehydrogenase; PDBTitle: role of packing defects in the evolution of allostery and induced fit2 in human udp-glucose dehydrogenase. Phyre2 PDB 1g94 chain A domain 1 Region: 79 - 88 Aligned: 10 Modelled: 10 Confidence: 6.6% Identity: 40% Fold: Glycosyl hydrolase domain Superfamily: Glycosyl hydrolase domain Family: alpha-Amylases, C-terminal beta-sheet domain Phyre2 PDB 1jae chain A domain 1 Region: 79 - 88 Aligned: 10 Modelled: 10 Confidence: 6.6% Identity: 40% Fold: Glycosyl hydrolase domain Superfamily: Glycosyl hydrolase domain Family: alpha-Amylases, C-terminal beta-sheet domain Phyre2 PDB 2bhl chain B Region: 58 - 85 Aligned: 20 Modelled: 28 Confidence: 6.6% Identity: 45% PDB header:oxidoreductase (choh(d)-nadp) Chain: B: PDB Molecule:glucose-6-phosphate 1-dehydrogenase; PDBTitle: x-ray structure of human glucose-6-phosphate dehydrogenase2 (deletion variant) complexed with glucose-6-phosphate Phyre2 PDB 1e0f chain I Region: 83 - 110 Aligned: 27 Modelled: 28 Confidence: 6.5% Identity: 37% PDB header:coagulation/crystal structure/heparin-b Chain: I: PDB Molecule:haemadin; PDBTitle: crystal structure of the human alpha-thrombin-haemadin2 complex: an exosite ii-binding inhibitor Phyre2 PDB 1e0f chain I Region: 83 - 110 Aligned: 27 Modelled: 28 Confidence: 6.5% Identity: 37% Fold: Knottins (small inhibitors, toxins, lectins) Superfamily: Leech antihemostatic proteins Family: Hirudin-like Phyre2 PDB 3dhp chain A domain 1 Region: 79 - 88 Aligned: 10 Modelled: 10 Confidence: 6.3% Identity: 40% Fold: Glycosyl hydrolase domain Superfamily: Glycosyl hydrolase domain Family: alpha-Amylases, C-terminal beta-sheet domain Phyre2 1 d1ig4a_ 2 c2a2bA_ 3 c2q3eH_ 4 d1flca1 5 d1ua7a1 6 c3ebkA_ 7 c2yqpA_ 8 d1q90r_ 9 c1q90R_ 10 c3plnA_ 11 d1qkia2 12 c3ggpA_ 13 d1hx0a1 14 c3prjB_ 15 d1g94a1 16 d1jaea1 17 c2bhlB_ 18 c1e0fI_ 19 d1e0fi_ 20 d3dhpa1 21 22 23 24 25 26 27 28 29

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1ig4a_ 50.9 32 Fold:DNA-binding domain Superfamily:DNA-binding domain Family:Methyl-CpG-binding domain, MBD 2 c2a2bA_ 49.6 41 PDB header:antibiotic Chain: A: PDB Molecule:bacteriocin curvacin a; PDBTitle: curvacin a 3 c2q3eH_ 16.6 29 PDB header:oxidoreductase Chain: H: PDB Molecule:udp-glucose 6-dehydrogenase; PDBTitle: structure of human udp-glucose dehydrogenase complexed with nadh and2 udp-glucose 4 d1flca1 11.7 45 Fold:Viral protein domain Superfamily:Viral protein domain Family:Hemagglutinin domain of haemagglutinin-esterase-fusion glycoprotein HEF1 5 d1ua7a1 11.0 10 Fold:Glycosyl hydrolase domain Superfamily:Glycosyl hydrolase domain Family:alpha-Amylases, C-terminal beta-sheet domain 6 c3ebkA_ 9.9 22 PDB header:allergen Chain: A: PDB Molecule:allergen bla g 4; PDBTitle: crystal structure of major allergens, bla g 4 from2 cockroaches 7 c2yqpA_ 8.5 44 PDB header:gene regulation, hydrolase Chain: A: PDB Molecule:probable atp-dependent rna helicase ddx59; PDBTitle: solution structure of the zf-hit domain in dead (asp-glu-2 ala-asp) box polypeptide 59 8 d1q90r_ 8.5 33 Fold:Single transmembrane helix Superfamily:ISP transmembrane anchor Family:ISP transmembrane anchor 9 c1q90R_ 8.5 33 PDB header:photosynthesis Chain: R: PDB Molecule:cytochrome b6-f complex iron-sulfur subunit; PDBTitle: structure of the cytochrome b6f (plastohydroquinone : plastocyanin2 oxidoreductase) from chlamydomonas reinhardtii 10 c3plnA_ 8.1 25 PDB header:oxidoreductase Chain: A: PDB Molecule:udp-glucose 6-dehydrogenase; PDBTitle: crystal structure of klebsiella pneumoniae udp-glucose 6-dehydrogenase2 complexed with udp-glucose 11 d1qkia2 7.6 45 Fold:FwdE/GAPDH domain-like Superfamily:Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain Family:Glucose 6-phosphate dehydrogenase-like 12 c3ggpA_ 7.5 19 PDB header:oxidoreductase Chain: A: PDB Molecule:prephenate dehydrogenase; PDBTitle: crystal structure of prephenate dehydrogenase from a. aeolicus in2 complex with hydroxyphenyl propionate and nad+ 13 d1hx0a1 7.1 40 Fold:Glycosyl hydrolase domain Superfamily:Glycosyl hydrolase domain Family:alpha-Amylases, C-terminal beta-sheet domain 14 c3prjB_ 7.0 29 PDB header:oxidoreductase Chain: B: PDB Molecule:udp-glucose 6-dehydrogenase; PDBTitle: role of packing defects in the evolution of allostery and induced fit2 in human udp-glucose dehydrogenase. 15 d1g94a1 6.6 40 Fold:Glycosyl hydrolase domain Superfamily:Glycosyl hydrolase domain Family:alpha-Amylases, C-terminal beta-sheet domain 16 d1jaea1 6.6 40 Fold:Glycosyl hydrolase domain Superfamily:Glycosyl hydrolase domain Family:alpha-Amylases, C-terminal beta-sheet domain 17 c2bhlB_ 6.6 45 PDB header:oxidoreductase (choh(d)-nadp) Chain: B: PDB Molecule:glucose-6-phosphate 1-dehydrogenase; PDBTitle: x-ray structure of human glucose-6-phosphate dehydrogenase2 (deletion variant) complexed with glucose-6-phosphate 18 c1e0fI_ 6.5 37 PDB header:coagulation/crystal structure/heparin-b Chain: I: PDB Molecule:haemadin; PDBTitle: crystal structure of the human alpha-thrombin-haemadin2 complex: an exosite ii-binding inhibitor 19 d1e0fi_ 6.5 37 Fold:Knottins (small inhibitors, toxins, lectins) Superfamily:Leech antihemostatic proteins Family:Hirudin-like 20 d3dhpa1 6.3 40 Fold:Glycosyl hydrolase domain Superfamily:Glycosyl hydrolase domain Family:alpha-Amylases, C-terminal beta-sheet domain 21 d1t27a_ not modelled 6.2 16 Fold:TBP-like Superfamily:Bet v1-like Family:Phoshatidylinositol transfer protein, PITP 22 d1r0va3 not modelled 6.1 29 Fold:MutS N-terminal domain-like Superfamily:tRNA-intron endonuclease N-terminal domain-like Family:tRNA-intron endonuclease N-terminal domain-like 23 d1rmka_ not modelled 6.0 45 Fold:Knottins (small inhibitors, toxins, lectins) Superfamily:omega toxin-like Family:Conotoxin 24 d1h9aa1 not modelled 6.0 33 Fold:NAD(P)-binding Rossmann-fold domains Superfamily:NAD(P)-binding Rossmann-fold domains Family:Glyceraldehyde-3-phosphate dehydrogenase-like, N-terminal domain 25 c2l3bA_ not modelled 5.9 29 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:conserved protein found in conjugate transposon; PDBTitle: solution nmr structure of the bt_0084 lipoprotein from bacteroides2 thetaiotaomicron, northeast structural genomics consortium target3 btr376 26 d2a1la1 not modelled 5.9 20 Fold:TBP-like Superfamily:Bet v1-like Family:Phoshatidylinositol transfer protein, PITP 27 c2l7qA_ not modelled 5.9 33 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:conserved protein found in conjugate transposon; PDBTitle: solution nmr structure of conjugate transposon protein bvu_1572(27-2 141) from bacteroides vulgatus, northeast structural genomics3 consortium target bvr155 28 c1e0fJ_ not modelled 5.8 37 PDB header:coagulation/crystal structure/heparin-b Chain: J: PDB Molecule:haemadin; PDBTitle: crystal structure of the human alpha-thrombin-haemadin2 complex: an exosite ii-binding inhibitor 29 c1qkiE_ not modelled 5.5 45 PDB header:oxidoreductase Chain: E: PDB Molecule:glucose-6-phosphate 1-dehydrogenase; PDBTitle: x-ray structure of human glucose 6-phosphate dehydrogenase2 (variant canton r459l) complexed with structural nadp+

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0