Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	P	E	S	L	M	V	I	R	S	S	S	T	L	R	K	H	W	E	W	M	T	F	S	A	D	S	V	S	S	V	H	T	L	T	D	D	L	P	L	E	S	L	A	D	Q	P	G	A	G	N	V	H	L	L	I	P	P	E	G
Secondary structure
SS confidence
Disorder		?	?	?	?																					?						?	?	?	?	?	?									?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		L	L	Y	R	S	L	T	L	P	N	A	K	Y	K	L	T	A	Q	T	L	Q	W	L	A	E	E	T	L	P	D	N	T	Q	D	W	H	W	T	V	V	D	K	Q	N	E	S	V	E	V	I	G	I	Q	S	E	K	L	S	R	Y
Secondary structure
SS confidence
Disorder									?		?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		L	E	R	L	H	T	A	G	L	N	V	T	R	V	L	P	D	G	C	Y	L	P	W	E	V	D	S	W	T	L	V	N	Q	Q	T	S	W	L	I	R	S	A	A	H	A	F	N	E	L	D	E	H	W	L	Q	H	L	A	A	Q
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		F	P	P	E	N	M	L	C	Y	G	V	V	P	H	G	V	A	A	A	N	P	L	I	Q	H	P	E	I	P	S	L	S	L	Y	S	A	D	I	A	F	Q	R	Y	D	M	L	H	G	I	F	R	K	Q	K	T	V	S	K	S	G
Secondary structure
SS confidence
Disorder				?	?		?		?	?		?	?	?	?	?	?	?	?	?	?	?	?	?																											?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		K	W	L	A	R	L	A	V	S	C	L	V	L	A	I	L	S	F	V	G	S	R	S	I	A	L	W	H	T	L	K	I	E	D	Q	L	Q	Q	Q	Q	Q	E	T	W	Q	R	Y	F	P	Q	I	K	R	T	H	N	F	R	F	Y
Secondary structure
SS confidence
Disorder		?																																																			?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		F	K	Q	Q	L	A	Q	Q	Y	P	E	A	V	P	L	L	Y	H	L	Q	T	L	L	L	E	H	P	E	L	Q	L	M	E	A	N	Y	S	Q	K	Q	K	S	L	T	L	K	M	S	A	K	S	E	A	N	I	D	R	F	C	E
Secondary structure
SS confidence
Disorder							?	?	?	?	?	?																?	?	?								?	?	?	?										?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.
Sequence		L	T	Q	S	W	L	P	M	E	K	T	E	K	D	P	V	S	G	V	W	T	V	R	N	S	G	K
Secondary structure
SS confidence
Disorder				?	?		?					?	?	?	?	?	?	?	?						?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1yf5 chain L Region: 3 - 231 Aligned: 229 Modelled: 229 Confidence: 100.0% Identity: 22% PDB header:transport protein Chain: L: PDB Molecule:general secretion pathway protein l; PDBTitle: cyto-epsl: the cytoplasmic domain of epsl, an inner membrane component2 of the type ii secretion system of vibrio cholerae Phyre2 PDB 2bh1 chain A domain 2 Region: 3 - 144 Aligned: 142 Modelled: 142 Confidence: 100.0% Identity: 24% Fold: Ribonuclease H-like motif Superfamily: Actin-like ATPase domain Family: Cyto-EpsL domain Phyre2 PDB 2w7v chain B Region: 311 - 385 Aligned: 75 Modelled: 73 Confidence: 99.8% Identity: 11% PDB header:transport protein Chain: B: PDB Molecule:general secretion pathway protein l; PDBTitle: periplasmic domain of epsl from vibrio parahaemolyticus Phyre2 PDB 2bh1 chain A domain 1 Region: 145 - 231 Aligned: 87 Modelled: 87 Confidence: 99.7% Identity: 18% Fold: Ribonuclease H-like motif Superfamily: Actin-like ATPase domain Family: Cyto-EpsL domain Phyre2 PDB 2ych chain A Region: 25 - 141 Aligned: 117 Modelled: 117 Confidence: 98.0% Identity: 16% PDB header:cell cycle Chain: A: PDB Molecule:competence protein pilm; PDBTitle: pilm-piln type iv pilus biogenesis complex Phyre2 PDB 1p6t chain A domain 2 Region: 317 - 379 Aligned: 61 Modelled: 63 Confidence: 27.1% Identity: 11% Fold: Ferredoxin-like Superfamily: HMA, heavy metal-associated domain Family: HMA, heavy metal-associated domain Phyre2 PDB 1s6u chain A Region: 317 - 375 Aligned: 57 Modelled: 59 Confidence: 25.7% Identity: 5% Fold: Ferredoxin-like Superfamily: HMA, heavy metal-associated domain Family: HMA, heavy metal-associated domain Phyre2 PDB 2l3m chain A Region: 317 - 369 Aligned: 51 Modelled: 53 Confidence: 21.0% Identity: 8% PDB header:metal binding protein Chain: A: PDB Molecule:copper-ion-binding protein; PDBTitle: solution structure of the putative copper-ion-binding protein from2 bacillus anthracis str. ames Phyre2 PDB 1e4f chain T domain 1 Region: 51 - 140 Aligned: 90 Modelled: 90 Confidence: 19.7% Identity: 6% Fold: Ribonuclease H-like motif Superfamily: Actin-like ATPase domain Family: Actin/HSP70 Phyre2 PDB 2qif chain A domain 1 Region: 317 - 368 Aligned: 50 Modelled: 52 Confidence: 16.9% Identity: 10% Fold: Ferredoxin-like Superfamily: HMA, heavy metal-associated domain Family: HMA, heavy metal-associated domain Phyre2 PDB 2aw0 chain A Region: 317 - 372 Aligned: 54 Modelled: 56 Confidence: 16.7% Identity: 4% Fold: Ferredoxin-like Superfamily: HMA, heavy metal-associated domain Family: HMA, heavy metal-associated domain Phyre2 PDB 2dyn chain A Region: 102 - 132 Aligned: 31 Modelled: 31 Confidence: 12.9% Identity: 16% Fold: PH domain-like barrel Superfamily: PH domain-like Family: Pleckstrin-homology domain (PH domain) Phyre2 PDB 1dcj chain A Region: 345 - 386 Aligned: 42 Modelled: 42 Confidence: 12.9% Identity: 19% Fold: IF3-like Superfamily: SirA-like Family: SirA-like Phyre2 PDB 1cpz chain A Region: 317 - 370 Aligned: 52 Modelled: 54 Confidence: 10.8% Identity: 4% Fold: Ferredoxin-like Superfamily: HMA, heavy metal-associated domain Family: HMA, heavy metal-associated domain Phyre2 PDB 1sb6 chain A Region: 317 - 369 Aligned: 47 Modelled: 53 Confidence: 10.4% Identity: 6% Fold: Ferredoxin-like Superfamily: HMA, heavy metal-associated domain Family: HMA, heavy metal-associated domain Phyre2 PDB 2ky4 chain A Region: 274 - 303 Aligned: 30 Modelled: 30 Confidence: 9.4% Identity: 3% PDB header:photosynthesis Chain: A: PDB Molecule:phycobilisome linker polypeptide; PDBTitle: solution nmr structure of the pbs linker domain of phycobilisome2 linker polypeptide from anabaena sp. northeast structural genomics3 consortium target nsr123e Phyre2 PDB 1uv7 chain A Region: 335 - 386 Aligned: 51 Modelled: 52 Confidence: 8.8% Identity: 6% Fold: RRF/tRNA synthetase additional domain-like Superfamily: General secretion pathway protein M, EpsM Family: General secretion pathway protein M, EpsM Phyre2 PDB 1uv7 chain A Region: 335 - 386 Aligned: 51 Modelled: 52 Confidence: 8.8% Identity: 6% PDB header:transport Chain: A: PDB Molecule:general secretion pathway protein m; PDBTitle: periplasmic domain of epsm from vibrio cholerae Phyre2 PDB 1p6t chain A domain 1 Region: 317 - 368 Aligned: 49 Modelled: 52 Confidence: 8.6% Identity: 12% Fold: Ferredoxin-like Superfamily: HMA, heavy metal-associated domain Family: HMA, heavy metal-associated domain Phyre2 PDB 3p14 chain C Region: 112 - 150 Aligned: 38 Modelled: 39 Confidence: 7.8% Identity: 16% PDB header:isomerase Chain: C: PDB Molecule:l-rhamnose isomerase; PDBTitle: crystal structure of l-rhamnose isomerase with a novel high thermo-2 stability from bacillus halodurans Phyre2 1 c1yf5L_ 2 d2bh1a2 3 c2w7vB_ 4 d2bh1a1 5 c2ychA_ 6 d1p6ta2 7 d1s6ua_ 8 c2l3mA_ 9 d1e4ft1 10 d2qifa1 11 d2aw0a_ 12 d2dyna_ 13 d1dcja_ 14 d1cpza_ 15 d1sb6a_ 16 c2ky4A_ 17 d1uv7a_ 18 c1uv7A_ 19 d1p6ta1 20 c3p14C_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c1yf5L_ 100.0 22 PDB header:transport protein Chain: L: PDB Molecule:general secretion pathway protein l; PDBTitle: cyto-epsl: the cytoplasmic domain of epsl, an inner membrane component2 of the type ii secretion system of vibrio cholerae 2 d2bh1a2 100.0 24 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Cyto-EpsL domain 3 c2w7vB_ 99.8 11 PDB header:transport protein Chain: B: PDB Molecule:general secretion pathway protein l; PDBTitle: periplasmic domain of epsl from vibrio parahaemolyticus 4 d2bh1a1 99.7 18 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Cyto-EpsL domain 5 c2ychA_ 98.0 16 PDB header:cell cycle Chain: A: PDB Molecule:competence protein pilm; PDBTitle: pilm-piln type iv pilus biogenesis complex 6 d1p6ta2 27.1 11 Fold:Ferredoxin-like Superfamily:HMA, heavy metal-associated domain Family:HMA, heavy metal-associated domain 7 d1s6ua_ 25.7 5 Fold:Ferredoxin-like Superfamily:HMA, heavy metal-associated domain Family:HMA, heavy metal-associated domain 8 c2l3mA_ 21.0 8 PDB header:metal binding protein Chain: A: PDB Molecule:copper-ion-binding protein; PDBTitle: solution structure of the putative copper-ion-binding protein from2 bacillus anthracis str. ames 9 d1e4ft1 19.7 6 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Actin/HSP70 10 d2qifa1 16.9 10 Fold:Ferredoxin-like Superfamily:HMA, heavy metal-associated domain Family:HMA, heavy metal-associated domain 11 d2aw0a_ 16.7 4 Fold:Ferredoxin-like Superfamily:HMA, heavy metal-associated domain Family:HMA, heavy metal-associated domain 12 d2dyna_ 12.9 16 Fold:PH domain-like barrel Superfamily:PH domain-like Family:Pleckstrin-homology domain (PH domain) 13 d1dcja_ 12.9 19 Fold:IF3-like Superfamily:SirA-like Family:SirA-like 14 d1cpza_ 10.8 4 Fold:Ferredoxin-like Superfamily:HMA, heavy metal-associated domain Family:HMA, heavy metal-associated domain 15 d1sb6a_ 10.4 6 Fold:Ferredoxin-like Superfamily:HMA, heavy metal-associated domain Family:HMA, heavy metal-associated domain 16 c2ky4A_ 9.4 3 PDB header:photosynthesis Chain: A: PDB Molecule:phycobilisome linker polypeptide; PDBTitle: solution nmr structure of the pbs linker domain of phycobilisome2 linker polypeptide from anabaena sp. northeast structural genomics3 consortium target nsr123e 17 d1uv7a_ 8.8 6 Fold:RRF/tRNA synthetase additional domain-like Superfamily:General secretion pathway protein M, EpsM Family:General secretion pathway protein M, EpsM 18 c1uv7A_ 8.8 6 PDB header:transport Chain: A: PDB Molecule:general secretion pathway protein m; PDBTitle: periplasmic domain of epsm from vibrio cholerae 19 d1p6ta1 8.6 12 Fold:Ferredoxin-like Superfamily:HMA, heavy metal-associated domain Family:HMA, heavy metal-associated domain 20 c3p14C_ 7.8 16 PDB header:isomerase Chain: C: PDB Molecule:l-rhamnose isomerase; PDBTitle: crystal structure of l-rhamnose isomerase with a novel high thermo-2 stability from bacillus halodurans 21 c2x29A_ not modelled 7.7 30 PDB header:cell adhesion Chain: A: PDB Molecule:tumor necrosis factor ligand superfamily member PDBTitle: crystal structure of human4-1bb ligand ectodomain 22 c3dxsX_ not modelled 7.7 11 PDB header:hydrolase Chain: X: PDB Molecule:copper-transporting atpase ran1; PDBTitle: crystal structure of a copper binding domain from hma7, a p-2 type atpase 23 d1d8wa_ not modelled 7.3 24 Fold:TIM beta/alpha-barrel Superfamily:Xylose isomerase-like Family:L-rhamnose isomerase 24 c2jpiA_ not modelled 7.2 6 PDB header:structural genomics Chain: A: PDB Molecule:hypothetical protein; PDBTitle: chemical shift assignments of pa4090 from pseudomonas2 aeruginosa 25 c2yciX_ not modelled 7.1 19 PDB header:transferase Chain: X: PDB Molecule:5-methyltetrahydrofolate corrinoid/iron sulfur protein PDBTitle: methyltransferase native 26 d1f44a1 not modelled 7.1 4 Fold:SAM domain-like Superfamily:lambda integrase-like, N-terminal domain Family:lambda integrase-like, N-terminal domain 27 c2k2pA_ not modelled 7.0 11 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein atu1203; PDBTitle: solution nmr structure of protein atu1203 from agrobacterium2 tumefaciens. northeast structural genomics consortium (nesg) target3 att10, ontario center for structural proteomics target atc1183 28 d1q8la_ not modelled 7.0 5 Fold:Ferredoxin-like Superfamily:HMA, heavy metal-associated domain Family:HMA, heavy metal-associated domain 29 c2dhiA_ not modelled 7.0 9 PDB header:signaling protein Chain: A: PDB Molecule:pleckstrin homology domain-containing family b PDBTitle: solution structure of the ph domain of evectin-2 from mouse 30 c2kkhA_ not modelled 6.6 10 PDB header:metal transport Chain: A: PDB Molecule:putative heavy metal transporter; PDBTitle: structure of the zinc binding domain of the atpase hma4 31 c1y3kA_ not modelled 5.9 5 PDB header:hydrolase Chain: A: PDB Molecule:copper-transporting atpase 1; PDBTitle: solution structure of the apo form of the fifth domain of2 menkes protein 32 d1afia_ not modelled 5.6 7 Fold:Ferredoxin-like Superfamily:HMA, heavy metal-associated domain Family:HMA, heavy metal-associated domain 33 c2rloA_ not modelled 5.6 6 PDB header:signaling protein Chain: A: PDB Molecule:centaurin-gamma 1; PDBTitle: split ph domain of pi3-kinase enhancer 34 c3lstB_ not modelled 5.4 26 PDB header:transferase Chain: B: PDB Molecule:calo1 methyltransferase; PDBTitle: crystal structure of calo1, methyltransferase in calicheamicin2 biosynthesis, sah bound form 35 d1f6ya_ not modelled 5.3 18 Fold:TIM beta/alpha-barrel Superfamily:Dihydropteroate synthetase-like Family:Methyltetrahydrofolate-utiluzing methyltransferases

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

---

Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0