Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	T	A	E	G	H	L	L	F	S	I	A	C	A	V	F	A	K	N	A	E	L	T	P	V	L	A	Q	G	D	W	W	H	I	V	P	S	A	I	L	T	C	L	L	P	D	I	D	H	P	K	S	F	L	G	Q	R	L	K	W
Secondary structure
SS confidence
Disorder		?	?	?																		?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		I	S	K	P	I	A	R	A	F	G	H	R	G	F	T	H	S	L	L	A	V	F	A	L	L	A	T	F	Y	L	K	V	P	E	G	W	F	I	P	A	D	A	L	Q	G	M	V	L	G	Y	L	S	H	I	L	A	D	M	L	T
Secondary structure
SS confidence
Disorder																																		?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		P	A	G	V	P	L	L	W	P	C	R	W	R	F	R	L	P	I	L	V	P	Q	K	G	N	Q	L	E	R	F	I	C	M	A	L	F	V	W	S	V	W	M	P	H	S	L	P	E	N	S	A	V	R	W	S	S	Q	M	I	N
Secondary structure
SS confidence
Disorder																						?																									?		?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.
Sequence		T	L	Q	I	Q	F	H	R	L	I	K	H	Q	V	E	Y
Secondary structure
SS confidence
Disorder															?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1ca1 chain A domain 1 Region: 104 - 122 Aligned: 19 Modelled: 19 Confidence: 40.5% Identity: 32% Fold: Phospholipase C/P1 nuclease Superfamily: Phospholipase C/P1 nuclease Family: Phospholipase C Phyre2 PDB 1olp chain A domain 1 Region: 104 - 121 Aligned: 18 Modelled: 18 Confidence: 31.2% Identity: 33% Fold: Phospholipase C/P1 nuclease Superfamily: Phospholipase C/P1 nuclease Family: Phospholipase C Phyre2 PDB 1gyg chain A Region: 106 - 122 Aligned: 17 Modelled: 17 Confidence: 26.9% Identity: 35% PDB header:hydrolase Chain: A: PDB Molecule:phospholipase c; PDBTitle: r32 closed form of alpha-toxin from clostridium perfringens2 strain cer89l43 Phyre2 PDB 1kho chain A domain 1 Region: 104 - 121 Aligned: 18 Modelled: 18 Confidence: 26.7% Identity: 33% Fold: Phospholipase C/P1 nuclease Superfamily: Phospholipase C/P1 nuclease Family: Phospholipase C Phyre2 PDB 1y2k chain A domain 1 Region: 5 - 51 Aligned: 43 Modelled: 47 Confidence: 18.8% Identity: 23% Fold: HD-domain/PDEase-like Superfamily: HD-domain/PDEase-like Family: PDEase Phyre2 PDB 1xot chain B Region: 5 - 51 Aligned: 43 Modelled: 47 Confidence: 18.0% Identity: 23% PDB header:hydrolase Chain: B: PDB Molecule:camp-specific 3',5'-cyclic phosphodiesterase 4b; PDBTitle: catalytic domain of human phosphodiesterase 4b in complex with2 vardenafil Phyre2 PDB 2r8q chain A Region: 5 - 51 Aligned: 43 Modelled: 47 Confidence: 13.8% Identity: 16% PDB header:hydrolase Chain: A: PDB Molecule:class i phosphodiesterase pdeb1; PDBTitle: structure of lmjpdeb1 in complex with ibmx Phyre2 PDB 3ecm chain A Region: 5 - 51 Aligned: 43 Modelled: 47 Confidence: 12.9% Identity: 26% PDB header:hydrolase Chain: A: PDB Molecule:high affinity camp-specific and ibmx-insensitive PDBTitle: crystal structure of the unliganded pde8a catalytic domain Phyre2 PDB 1z1l chain A Region: 5 - 51 Aligned: 43 Modelled: 47 Confidence: 12.8% Identity: 30% PDB header:hydrolase Chain: A: PDB Molecule:cgmp-dependent 3',5'-cyclic phosphodiesterase; PDBTitle: the crystal structure of the phosphodiesterase 2a catalytic2 domain Phyre2 PDB 3ec2 chain A Region: 2 - 12 Aligned: 11 Modelled: 11 Confidence: 11.9% Identity: 18% PDB header:replication Chain: A: PDB Molecule:dna replication protein dnac; PDBTitle: crystal structure of the dnac helicase loader Phyre2 PDB 3dy8 chain A domain 1 Region: 5 - 51 Aligned: 43 Modelled: 47 Confidence: 11.5% Identity: 19% Fold: HD-domain/PDEase-like Superfamily: HD-domain/PDEase-like Family: PDEase Phyre2 PDB 1ah7 chain A Region: 104 - 122 Aligned: 19 Modelled: 19 Confidence: 11.2% Identity: 32% Fold: Phospholipase C/P1 nuclease Superfamily: Phospholipase C/P1 nuclease Family: Phospholipase C Phyre2 PDB 1f0j chain A Region: 5 - 51 Aligned: 43 Modelled: 47 Confidence: 11.1% Identity: 23% Fold: HD-domain/PDEase-like Superfamily: HD-domain/PDEase-like Family: PDEase Phyre2 PDB 3bjc chain A Region: 12 - 51 Aligned: 36 Modelled: 40 Confidence: 11.0% Identity: 17% PDB header:hydrolase Chain: A: PDB Molecule:cgmp-specific 3',5'-cyclic phosphodiesterase; PDBTitle: crystal structure of the pde5a catalytic domain in complex2 with a novel inhibitor Phyre2 PDB 2w58 chain B Region: 2 - 12 Aligned: 11 Modelled: 11 Confidence: 10.4% Identity: 36% PDB header:hydrolase Chain: B: PDB Molecule:primosome component (helicase loader); PDBTitle: crystal structure of the dnai Phyre2 PDB 2qgz chain A Region: 2 - 12 Aligned: 11 Modelled: 11 Confidence: 10.4% Identity: 27% PDB header:hydrolase Chain: A: PDB Molecule:putative primosome component; PDBTitle: crystal structure of a putative primosome component from2 streptococcus pyogenes serotype m3. northeast structural3 genomics target dr58 Phyre2 PDB 3cvg chain C Region: 115 - 133 Aligned: 19 Modelled: 19 Confidence: 9.5% Identity: 21% PDB header:metal binding protein Chain: C: PDB Molecule:putative metal binding protein; PDBTitle: crystal structure of a periplasmic putative metal binding protein Phyre2 PDB 1p3q chain Q Region: 40 - 48 Aligned: 9 Modelled: 4 Confidence: 9.0% Identity: 44% Fold: RuvA C-terminal domain-like Superfamily: UBA-like Family: CUE domain Phyre2 PDB 1tbf chain A Region: 5 - 51 Aligned: 43 Modelled: 47 Confidence: 8.9% Identity: 19% Fold: HD-domain/PDEase-like Superfamily: HD-domain/PDEase-like Family: PDEase Phyre2 PDB 1xoz chain A Region: 5 - 51 Aligned: 43 Modelled: 47 Confidence: 8.9% Identity: 19% PDB header:hydrolase Chain: A: PDB Molecule:cgmp-specific 3',5'-cyclic phosphodiesterase; PDBTitle: catalytic domain of human phosphodiesterase 5a in complex2 with tadalafil Phyre2 1 d1ca1a1 2 d1olpa1 3 c1gygA_ 4 d1khoa1 5 d1y2ka1 6 c1xotB_ 7 c2r8qA_ 8 c3ecmA_ 9 c1z1lA_ 10 c3ec2A_ 11 d3dy8a1 12 d1ah7a_ 13 d1f0ja_ 14 c3bjcA_ 15 c2w58B_ 16 c2qgzA_ 17 c3cvgC_ 18 d1p3qq_ 19 d1tbfa_ 20 c1xozA_ 21 22 23 24 25 26 27 28 29 30 31 32 33

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1ca1a1 40.5 32 Fold:Phospholipase C/P1 nuclease Superfamily:Phospholipase C/P1 nuclease Family:Phospholipase C 2 d1olpa1 31.2 33 Fold:Phospholipase C/P1 nuclease Superfamily:Phospholipase C/P1 nuclease Family:Phospholipase C 3 c1gygA_ 26.9 35 PDB header:hydrolase Chain: A: PDB Molecule:phospholipase c; PDBTitle: r32 closed form of alpha-toxin from clostridium perfringens2 strain cer89l43 4 d1khoa1 26.7 33 Fold:Phospholipase C/P1 nuclease Superfamily:Phospholipase C/P1 nuclease Family:Phospholipase C 5 d1y2ka1 18.8 23 Fold:HD-domain/PDEase-like Superfamily:HD-domain/PDEase-like Family:PDEase 6 c1xotB_ 18.0 23 PDB header:hydrolase Chain: B: PDB Molecule:camp-specific 3',5'-cyclic phosphodiesterase 4b; PDBTitle: catalytic domain of human phosphodiesterase 4b in complex with2 vardenafil 7 c2r8qA_ 13.8 16 PDB header:hydrolase Chain: A: PDB Molecule:class i phosphodiesterase pdeb1; PDBTitle: structure of lmjpdeb1 in complex with ibmx 8 c3ecmA_ 12.9 26 PDB header:hydrolase Chain: A: PDB Molecule:high affinity camp-specific and ibmx-insensitive PDBTitle: crystal structure of the unliganded pde8a catalytic domain 9 c1z1lA_ 12.8 30 PDB header:hydrolase Chain: A: PDB Molecule:cgmp-dependent 3',5'-cyclic phosphodiesterase; PDBTitle: the crystal structure of the phosphodiesterase 2a catalytic2 domain 10 c3ec2A_ 11.9 18 PDB header:replication Chain: A: PDB Molecule:dna replication protein dnac; PDBTitle: crystal structure of the dnac helicase loader 11 d3dy8a1 11.5 19 Fold:HD-domain/PDEase-like Superfamily:HD-domain/PDEase-like Family:PDEase 12 d1ah7a_ 11.2 32 Fold:Phospholipase C/P1 nuclease Superfamily:Phospholipase C/P1 nuclease Family:Phospholipase C 13 d1f0ja_ 11.1 23 Fold:HD-domain/PDEase-like Superfamily:HD-domain/PDEase-like Family:PDEase 14 c3bjcA_ 11.0 17 PDB header:hydrolase Chain: A: PDB Molecule:cgmp-specific 3',5'-cyclic phosphodiesterase; PDBTitle: crystal structure of the pde5a catalytic domain in complex2 with a novel inhibitor 15 c2w58B_ 10.4 36 PDB header:hydrolase Chain: B: PDB Molecule:primosome component (helicase loader); PDBTitle: crystal structure of the dnai 16 c2qgzA_ 10.4 27 PDB header:hydrolase Chain: A: PDB Molecule:putative primosome component; PDBTitle: crystal structure of a putative primosome component from2 streptococcus pyogenes serotype m3. northeast structural3 genomics target dr58 17 c3cvgC_ 9.5 21 PDB header:metal binding protein Chain: C: PDB Molecule:putative metal binding protein; PDBTitle: crystal structure of a periplasmic putative metal binding protein 18 d1p3qq_ 9.0 44 Fold:RuvA C-terminal domain-like Superfamily:UBA-like Family:CUE domain 19 d1tbfa_ 8.9 19 Fold:HD-domain/PDEase-like Superfamily:HD-domain/PDEase-like Family:PDEase 20 c1xozA_ 8.9 19 PDB header:hydrolase Chain: A: PDB Molecule:cgmp-specific 3',5'-cyclic phosphodiesterase; PDBTitle: catalytic domain of human phosphodiesterase 5a in complex2 with tadalafil 21 c1olpB_ not modelled 8.4 32 PDB header:hydrolase Chain: B: PDB Molecule:alpha-toxin; PDBTitle: alpha toxin from clostridium absonum 22 c3qi4A_ not modelled 8.4 19 PDB header:hydrolase Chain: A: PDB Molecule:high affinity cgmp-specific 3',5'-cyclic phosphodiesterase PDBTitle: crystal structure of pde9a(q453e) in complex with ibmx 23 c1wqdA_ not modelled 8.3 56 PDB header:toxin Chain: A: PDB Molecule:omtx2; PDBTitle: an unusual fold for potassium channel blockers: nmr2 structure of three toxins from the scorpion opisthacanthus3 madagascariensis 24 c1zklA_ not modelled 8.1 21 PDB header:hydrolase Chain: A: PDB Molecule:high-affinity camp-specific 3',5'-cyclic PDBTitle: multiple determinants for inhibitor selectivity of cyclic2 nucleotide phosphodiesterases 25 d2h44a1 not modelled 7.7 16 Fold:HD-domain/PDEase-like Superfamily:HD-domain/PDEase-like Family:PDEase 26 c2kjqA_ not modelled 7.6 27 PDB header:replication Chain: A: PDB Molecule:dnaa-related protein; PDBTitle: solution structure of protein nmb1076 from neisseria meningitidis.2 northeast structural genomics consortium target mr101b. 27 c3g3nA_ not modelled 7.4 21 PDB header:hydrolase Chain: A: PDB Molecule:high affinity camp-specific 3',5'-cyclic PDBTitle: pde7a catalytic domain in complex with 3-(2,6-2 difluorophenyl)-2-(methylthio)quinazolin-4(3h)-one 28 d1so2a_ not modelled 7.3 24 Fold:HD-domain/PDEase-like Superfamily:HD-domain/PDEase-like Family:PDEase 29 d1p3qr_ not modelled 7.0 43 Fold:RuvA C-terminal domain-like Superfamily:UBA-like Family:CUE domain 30 c2lf6A_ not modelled 6.8 23 PDB header:signaling protein Chain: A: PDB Molecule:effector protein hopab1; PDBTitle: solution nmr structure of hopabpph1448_220_320 from pseudomonas2 syringae pv. phaseolicola str. 1448a, midwest center for structural3 genomics target apc40132.4 and northeast structural genomics4 consortium target pst3a 31 d1mn3a_ not modelled 6.1 44 Fold:RuvA C-terminal domain-like Superfamily:UBA-like Family:CUE domain 32 c3ibjB_ not modelled 5.9 30 PDB header:hydrolase Chain: B: PDB Molecule:cgmp-dependent 3',5'-cyclic phosphodiesterase; PDBTitle: x-ray structure of pde2a 33 c2o8hA_ not modelled 5.4 18 PDB header:hydrolase Chain: A: PDB Molecule:phosphodiesterase-10a; PDBTitle: crystal structure of the catalytic domain of rat2 phosphodiesterase 10a

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0