Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	R	Y	S	K	L	T	M	L	I	P	C	A	L	L	L	S	A	C	T	T	V	T	P	A	Y	K	D	N	G	T	R	S	G	P	C	V	E	G	G	P	D	N	V	A	Q	Q	F	Y	D	Y	R	I	L	H	R	S	N	D	I
Secondary structure
SS confidence
Disorder		?	?	?	?		?											?							?			?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		T	A	L	R	P	Y	L	S	D	K	L	A	T	L	L	S	D	A	S	R	D	N	N	H	R	E	L	L	T	N	D	P	F	S	S	R	T	T	L	P	D	S	A	H	V	A	S	A	S	T	I	P	N	R	D	A	R	N	I	P
Secondary structure
SS confidence
Disorder																				?	?	?	?	?	?	?	?	?		?					?	?						?	?			?	?	?		?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.
Sequence		L	R	V	D	L	K	Q	G	D	Q	G	W	Q	D	E	V	L	M	I	Q	E	G	Q	C	W	V	I	D	D	V	R	Y	L	G	G	S	V	H	A	T	A	G	T	L	R	Q	S	I	E	N	R
Secondary structure
SS confidence
Disorder							?		?	?	?	?																									?	?	?	?	?	?	?	?					?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2qgu chain A Region: 93 - 156 Aligned: 64 Modelled: 64 Confidence: 84.4% Identity: 11% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:probable signal peptide protein; PDBTitle: three-dimensional structure of the phospholipid-binding protein from2 ralstonia solanacearum q8xv73_ralsq in complex with a phospholipid at3 the resolution 1.53 a. northeast structural genomics consortium4 target rsr89 Phyre2 PDB 3duk chain D Region: 104 - 153 Aligned: 47 Modelled: 50 Confidence: 78.3% Identity: 9% PDB header:unknown function Chain: D: PDB Molecule:ntf2-like protein of unknown function; PDBTitle: crystal structure of a ntf2-like protein of unknown function2 (mfla_0564) from methylobacillus flagellatus kt at 2.200 a resolution Phyre2 PDB 2cw9 chain A domain 1 Region: 57 - 154 Aligned: 94 Modelled: 98 Confidence: 77.9% Identity: 16% Fold: Cystatin-like Superfamily: NTF2-like Family: TIM44-like Phyre2 PDB 1vqq chain A domain 1 Region: 92 - 148 Aligned: 56 Modelled: 57 Confidence: 76.0% Identity: 11% Fold: Cystatin-like Superfamily: NTF2-like Family: Penicillin binding protein 2a (PBP2A), N-terminal domain Phyre2 PDB 3qk9 chain B Region: 57 - 154 Aligned: 94 Modelled: 98 Confidence: 75.0% Identity: 11% PDB header:protein transport Chain: B: PDB Molecule:mitochondrial import inner membrane translocase subunit PDBTitle: yeast tim44 c-terminal domain complexed with cymal-3 Phyre2 PDB 3fka chain D Region: 98 - 153 Aligned: 52 Modelled: 56 Confidence: 71.4% Identity: 17% PDB header:unknown function Chain: D: PDB Molecule:uncharacterized ntf-2 like protein; PDBTitle: crystal structure of a ntf-2 like protein of unknown function2 (spo1084) from silicibacter pomeroyi dss-3 at 1.69 a resolution Phyre2 PDB 3blz chain A domain 1 Region: 104 - 153 Aligned: 47 Modelled: 50 Confidence: 64.6% Identity: 11% Fold: Cystatin-like Superfamily: NTF2-like Family: Sbal0622-like Phyre2 PDB 1w8o chain A domain 3 Region: 84 - 145 Aligned: 61 Modelled: 62 Confidence: 50.6% Identity: 10% Fold: 6-bladed beta-propeller Superfamily: Sialidases Family: Sialidases (neuraminidases) Phyre2 PDB 2fxt chain A domain 1 Region: 57 - 127 Aligned: 66 Modelled: 71 Confidence: 35.3% Identity: 9% Fold: Cystatin-like Superfamily: NTF2-like Family: TIM44-like Phyre2 PDB 1dmu chain A Region: 106 - 145 Aligned: 39 Modelled: 40 Confidence: 32.1% Identity: 10% Fold: Restriction endonuclease-like Superfamily: Restriction endonuclease-like Family: Restriction endonuclease BglI Phyre2 PDB 3f7s chain A Region: 93 - 164 Aligned: 72 Modelled: 72 Confidence: 31.5% Identity: 17% PDB header:unknown function Chain: A: PDB Molecule:uncharacterized ntf2-like protein; PDBTitle: crystal structure of a ntf2-like protein of unknown function (pp_4556)2 from pseudomonas putida kt2440 at 2.11 a resolution Phyre2 PDB 2ber chain A Region: 84 - 145 Aligned: 60 Modelled: 62 Confidence: 22.7% Identity: 8% PDB header:hydrolase Chain: A: PDB Molecule:bacterial sialidase; PDBTitle: y370g active site mutant of the sialidase from2 micromonospora viridifaciens in complex with beta-neu5ac3 (sialic acid). Phyre2 PDB 1m98 chain A domain 2 Region: 22 - 63 Aligned: 40 Modelled: 42 Confidence: 21.6% Identity: 20% Fold: Cystatin-like Superfamily: NTF2-like Family: Orange carotenoid protein, C-terminal domain Phyre2 PDB 3gwr chain A Region: 37 - 147 Aligned: 96 Modelled: 93 Confidence: 20.3% Identity: 18% PDB header:protein binding Chain: A: PDB Molecule:putative calcium/calmodulin-dependent protein kinase type PDBTitle: crystal structure of putative calcium/calmodulin-dependent protein2 kinase type ii association domain (yp_315894.1) from thiobacillus3 denitrificans atcc 25259 at 2.00 a resolution Phyre2 PDB 3k7c chain C Region: 55 - 147 Aligned: 90 Modelled: 93 Confidence: 16.1% Identity: 11% PDB header:protein binding Chain: C: PDB Molecule:putative ntf2-like transpeptidase; PDBTitle: crystal structure of putative ntf2-like transpeptidase (np_281412.1)2 from campylobacter jejuni at 2.00 a resolution Phyre2 PDB 1kke chain A domain 2 Region: 85 - 160 Aligned: 55 Modelled: 76 Confidence: 14.9% Identity: 35% Fold: Virus attachment protein globular domain Superfamily: Virus attachment protein globular domain Family: Reovirus attachment protein sigma 1 head domain Phyre2 PDB 2j2z chain A domain 2 Region: 129 - 149 Aligned: 21 Modelled: 21 Confidence: 13.2% Identity: 19% Fold: C2 domain-like Superfamily: Periplasmic chaperone C-domain Family: Periplasmic chaperone C-domain Phyre2 PDB 1ub1 chain A Region: 130 - 155 Aligned: 26 Modelled: 26 Confidence: 12.7% Identity: 19% Fold: DNA-binding domain Superfamily: DNA-binding domain Family: Methyl-CpG-binding domain, MBD Phyre2 PDB 1kke chain A Region: 85 - 160 Aligned: 55 Modelled: 76 Confidence: 12.5% Identity: 35% PDB header:viral protein Chain: A: PDB Molecule:sigma 1 protein; PDBTitle: crystal structure of reovirus attachment protein sigma12 trimer Phyre2 PDB 3cnx chain A domain 1 Region: 102 - 147 Aligned: 46 Modelled: 46 Confidence: 10.7% Identity: 9% Fold: Cystatin-like Superfamily: NTF2-like Family: SAV4671-like Phyre2 1 c2qguA_ 2 c3dukD_ 3 d2cw9a1 4 d1vqqa1 5 c3qk9B_ 6 c3fkaD_ 7 d3blza1 8 d1w8oa3 9 d2fxta1 10 d1dmua_ 11 c3f7sA_ 12 c2berA_ 13 d1m98a2 14 c3gwrA_ 15 c3k7cC_ 16 d1kkea2 17 d2j2za2 18 d1ub1a_ 19 c1kkeA_ 20 d3cnxa1 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c2qguA_ 84.4 11 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:probable signal peptide protein; PDBTitle: three-dimensional structure of the phospholipid-binding protein from2 ralstonia solanacearum q8xv73_ralsq in complex with a phospholipid at3 the resolution 1.53 a. northeast structural genomics consortium4 target rsr89 2 c3dukD_ 78.3 9 PDB header:unknown function Chain: D: PDB Molecule:ntf2-like protein of unknown function; PDBTitle: crystal structure of a ntf2-like protein of unknown function2 (mfla_0564) from methylobacillus flagellatus kt at 2.200 a resolution 3 d2cw9a1 77.9 16 Fold:Cystatin-like Superfamily:NTF2-like Family:TIM44-like 4 d1vqqa1 76.0 11 Fold:Cystatin-like Superfamily:NTF2-like Family:Penicillin binding protein 2a (PBP2A), N-terminal domain 5 c3qk9B_ 75.0 11 PDB header:protein transport Chain: B: PDB Molecule:mitochondrial import inner membrane translocase subunit PDBTitle: yeast tim44 c-terminal domain complexed with cymal-3 6 c3fkaD_ 71.4 17 PDB header:unknown function Chain: D: PDB Molecule:uncharacterized ntf-2 like protein; PDBTitle: crystal structure of a ntf-2 like protein of unknown function2 (spo1084) from silicibacter pomeroyi dss-3 at 1.69 a resolution 7 d3blza1 64.6 11 Fold:Cystatin-like Superfamily:NTF2-like Family:Sbal0622-like 8 d1w8oa3 50.6 10 Fold:6-bladed beta-propeller Superfamily:Sialidases Family:Sialidases (neuraminidases) 9 d2fxta1 35.3 9 Fold:Cystatin-like Superfamily:NTF2-like Family:TIM44-like 10 d1dmua_ 32.1 10 Fold:Restriction endonuclease-like Superfamily:Restriction endonuclease-like Family:Restriction endonuclease BglI 11 c3f7sA_ 31.5 17 PDB header:unknown function Chain: A: PDB Molecule:uncharacterized ntf2-like protein; PDBTitle: crystal structure of a ntf2-like protein of unknown function (pp_4556)2 from pseudomonas putida kt2440 at 2.11 a resolution 12 c2berA_ 22.7 8 PDB header:hydrolase Chain: A: PDB Molecule:bacterial sialidase; PDBTitle: y370g active site mutant of the sialidase from2 micromonospora viridifaciens in complex with beta-neu5ac3 (sialic acid). 13 d1m98a2 21.6 20 Fold:Cystatin-like Superfamily:NTF2-like Family:Orange carotenoid protein, C-terminal domain 14 c3gwrA_ 20.3 18 PDB header:protein binding Chain: A: PDB Molecule:putative calcium/calmodulin-dependent protein kinase type PDBTitle: crystal structure of putative calcium/calmodulin-dependent protein2 kinase type ii association domain (yp_315894.1) from thiobacillus3 denitrificans atcc 25259 at 2.00 a resolution 15 c3k7cC_ 16.1 11 PDB header:protein binding Chain: C: PDB Molecule:putative ntf2-like transpeptidase; PDBTitle: crystal structure of putative ntf2-like transpeptidase (np_281412.1)2 from campylobacter jejuni at 2.00 a resolution 16 d1kkea2 14.9 35 Fold:Virus attachment protein globular domain Superfamily:Virus attachment protein globular domain Family:Reovirus attachment protein sigma 1 head domain 17 d2j2za2 13.2 19 Fold:C2 domain-like Superfamily:Periplasmic chaperone C-domain Family:Periplasmic chaperone C-domain 18 d1ub1a_ 12.7 19 Fold:DNA-binding domain Superfamily:DNA-binding domain Family:Methyl-CpG-binding domain, MBD 19 c1kkeA_ 12.5 35 PDB header:viral protein Chain: A: PDB Molecule:sigma 1 protein; PDBTitle: crystal structure of reovirus attachment protein sigma12 trimer 20 d3cnxa1 10.7 9 Fold:Cystatin-like Superfamily:NTF2-like Family:SAV4671-like 21 c1mwuA_ not modelled 10.1 11 PDB header:biosynthetic protein Chain: A: PDB Molecule:penicillin-binding protein 2a; PDBTitle: structure of methicillin acyl-penicillin binding protein 2a2 from methicillin resistant staphylococcus aureus strain3 27r at 2.60 a resolution. 22 d2duya1 not modelled 9.9 27 Fold:SAM domain-like Superfamily:RuvA domain 2-like Family:ComEA-like 23 d2r4ia1 not modelled 9.7 9 Fold:Cystatin-like Superfamily:NTF2-like Family:CHU142-like 24 d1g2qa_ not modelled 9.6 15 Fold:PRTase-like Superfamily:PRTase-like Family:Phosphoribosyltransferases (PRTases) 25 c2zxkB_ not modelled 9.4 24 PDB header:oxidoreductase Chain: B: PDB Molecule:red chlorophyll catabolite reductase, PDBTitle: crystal structure of semet-red chlorophyll catabolite2 reductase 26 c3lo2A_ not modelled 9.2 35 PDB header:antimicrobial protein Chain: A: PDB Molecule:neutrophil defensin 1; PDBTitle: crystal structure of human alpha-defensin 1 (y21a mutant) 27 c3lo2B_ not modelled 9.2 35 PDB header:antimicrobial protein Chain: B: PDB Molecule:neutrophil defensin 1; PDBTitle: crystal structure of human alpha-defensin 1 (y21a mutant) 28 c3gzrA_ not modelled 9.2 16 PDB header:unknown function Chain: A: PDB Molecule:uncharacterized protein with a ntf2-like fold; PDBTitle: crystal structure of an uncharacterized protein with a cystatin-like2 fold (cc_2572) from caulobacter vibrioides at 1.40 a resolution 29 c2veeC_ not modelled 9.1 12 PDB header:transport protein Chain: C: PDB Molecule:protoglobin; PDBTitle: structure of protoglobin from methanosarcina acetivorans2 c2a 30 d1ea9c2 not modelled 8.5 23 Fold:Glycosyl hydrolase domain Superfamily:Glycosyl hydrolase domain Family:alpha-Amylases, C-terminal beta-sheet domain 31 c2e26A_ not modelled 7.8 13 PDB header:signaling protein Chain: A: PDB Molecule:reelin; PDBTitle: crystal structure of two repeat fragment of reelin 32 c3s6xA_ not modelled 7.8 35 PDB header:viral protein Chain: A: PDB Molecule:outer capsid protein sigma-1; PDBTitle: structure of reovirus attachment protein sigma1 in complex with alpha-2 2,3-sialyllactose 33 d1sf9a_ not modelled 7.2 11 Fold:SH3-like barrel Superfamily:Hypothetical protein YfhH Family:Hypothetical protein YfhH 34 c2v6yA_ not modelled 7.1 30 PDB header:hydrolase Chain: A: PDB Molecule:aaa family atpase, p60 katanin; PDBTitle: structure of the mit domain from a s. solfataricus vps4-2 like atpase 35 d1kt0a2 not modelled 6.8 19 Fold:FKBP-like Superfamily:FKBP-like Family:FKBP immunophilin/proline isomerase 36 c3ke7A_ not modelled 6.7 11 PDB header:isomerase Chain: A: PDB Molecule:putative ketosteroid isomerase; PDBTitle: crystal structure of putative ketosteroid isomerase (yp_001303366.1)2 from parabacteroides distasonis atcc 8503 at 1.45 a resolution 37 d2gxfa1 not modelled 6.7 8 Fold:Cystatin-like Superfamily:NTF2-like Family:YybH-like 38 c3lo1A_ not modelled 6.6 35 PDB header:antimicrobial protein Chain: A: PDB Molecule:neutrophil defensin 1; PDBTitle: crystal structure of human alpha-defensin 1 (y16a mutant) 39 d3bb9a1 not modelled 6.6 14 Fold:Cystatin-like Superfamily:NTF2-like Family:SO0125-like 40 c3ie4A_ not modelled 6.3 15 PDB header:immune system Chain: A: PDB Molecule:gram-negative binding protein 3; PDBTitle: b-glucan binding domain of drosophila gnbp3 defines a novel2 family of pattern recognition receptor 41 c2ky8A_ not modelled 5.6 27 PDB header:transcription/dna Chain: A: PDB Molecule:methyl-cpg-binding domain protein 2; PDBTitle: solution structure and dynamic analysis of chicken mbd2 methyl binding2 domain bound to a target methylated dna sequence 42 d2dloa2 not modelled 5.5 43 Fold:Glucocorticoid receptor-like (DNA-binding domain) Superfamily:Glucocorticoid receptor-like (DNA-binding domain) Family:LIM domain 43 d3b7ca1 not modelled 5.5 16 Fold:Cystatin-like Superfamily:NTF2-like Family:SO0125-like 44 c2vtwF_ not modelled 5.3 19 PDB header:viral protein Chain: F: PDB Molecule:fiber protein 2; PDBTitle: structure of the c-terminal head domain of the fowl2 adenovirus type 1 short fibre

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0