Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	L	M	I	T	S	F	A	N	P	R	V	A	Q	A	F	V	D	Y	M	A	T	Q	G	V	I	L	T	I	Q	Q	H	N	Q	S	D	V	W	L	A	D	E	S	Q	A	E	R	V	R	A	E	L	A	R	F	L	E	N	P	A
Secondary structure
SS confidence
Disorder		?	?	?			?	?																									?			?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		D	P	R	Y	L	A	A	S	W	Q	A	G	H	T	G	S	G	L	H	Y	R	R	Y	P	F	F	A	A	L	R	E	R	A	G	P	V	T	W	V	M	M	I	A	C	V	V	V	F	I	A	M	Q	I	L	G	D	Q	E	V	M
Secondary structure
SS confidence
Disorder									?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?				?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		L	W	L	A	W	P	F	D	P	T	L	K	F	E	F	W	R	Y	F	T	H	A	L	M	H	F	S	L	M	H	I	L	F	N	L	L	W	W	W	Y	L	G	G	A	V	E	K	R	L	G	S	G	K	L	I	V	I	T	L	I
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		S	A	L	L	S	G	Y	V	Q	Q	K	F	S	G	P	W	F	G	G	L	S	G	V	V	Y	A	L	M	G	Y	V	W	L	R	G	E	R	D	P	Q	S	G	I	Y	L	Q	R	G	L	I	I	F	A	L	I	W	I	V	A	G
Secondary structure
SS confidence
Disorder																																								?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.
Sequence		W	F	D	L	F	G	M	S	M	A	N	G	A	H	I	A	G	L	A	V	G	L	A	M	A	F	V	D	S	L	N	A	R	K	R	K
Secondary structure
SS confidence
Disorder					?	?	?																							?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 3b45 chain A domain 1 Region: 91 - 270 Aligned: 180 Modelled: 180 Confidence: 100.0% Identity: 100% Fold: Rhomboid-like Superfamily: Rhomboid-like Family: Rhomboid-like Phyre2 PDB 2nr9 chain A domain 1 Region: 90 - 275 Aligned: 186 Modelled: 186 Confidence: 100.0% Identity: 36% Fold: Rhomboid-like Superfamily: Rhomboid-like Family: Rhomboid-like Phyre2 PDB 2gqc chain A Region: 1 - 57 Aligned: 57 Modelled: 57 Confidence: 98.6% Identity: 25% PDB header:hydrolase Chain: A: PDB Molecule:rhomboid intramembrane protease; PDBTitle: solution structure of the n-terminal domain of rhomboid intramembrane2 protease from p. aeruginosa Phyre2 PDB 1uta chain A Region: 1 - 62 Aligned: 62 Modelled: 62 Confidence: 93.9% Identity: 19% Fold: Ferredoxin-like Superfamily: Sporulation related repeat Family: Sporulation related repeat Phyre2 PDB 1x60 chain A Region: 1 - 61 Aligned: 61 Modelled: 61 Confidence: 93.5% Identity: 15% PDB header:hydrolase Chain: A: PDB Molecule:sporulation-specific n-acetylmuramoyl-l-alanine PDBTitle: solution structure of the peptidoglycan binding domain of2 b. subtilis cell wall lytic enzyme cwlc Phyre2 PDB 2y9j chain T Region: 9 - 54 Aligned: 46 Modelled: 46 Confidence: 93.5% Identity: 4% PDB header:protein transport Chain: T: PDB Molecule:protein prgh; PDBTitle: three-dimensional model of salmonella's needle complex at2 subnanometer resolution Phyre2 PDB 1yj7 chain A Region: 9 - 54 Aligned: 46 Modelled: 46 Confidence: 92.7% Identity: 9% PDB header:protein transport Chain: A: PDB Molecule:escj; PDBTitle: crystal structure of enteropathogenic e.coli (epec) type iii secretion2 system protein escj Phyre2 PDB 1zhv chain A domain 2 Region: 6 - 54 Aligned: 49 Modelled: 49 Confidence: 60.8% Identity: 16% Fold: Ferredoxin-like Superfamily: ACT-like Family: Atu0741-like Phyre2 PDB 2hfv chain A Region: 1 - 55 Aligned: 55 Modelled: 55 Confidence: 53.8% Identity: 15% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical protein rpa1041; PDBTitle: solution nmr structure of protein rpa1041 from pseudomonas2 aeruginosa. northeast structural genomics consortium3 target pat90. Phyre2 PDB 1zvp chain A domain 2 Region: 14 - 57 Aligned: 44 Modelled: 44 Confidence: 51.5% Identity: 18% Fold: Ferredoxin-like Superfamily: ACT-like Family: VC0802-like Phyre2 PDB 2hmf chain A domain 2 Region: 8 - 58 Aligned: 51 Modelled: 51 Confidence: 44.3% Identity: 18% Fold: Ferredoxin-like Superfamily: ACT-like Family: Aspartokinase allosteric domain-like Phyre2 PDB 2hfv chain A domain 1 Region: 1 - 54 Aligned: 54 Modelled: 54 Confidence: 37.7% Identity: 15% Fold: Ferredoxin-like Superfamily: GlnB-like Family: RPA1041-like Phyre2 PDB 1zvp chain B Region: 14 - 57 Aligned: 44 Modelled: 44 Confidence: 30.6% Identity: 18% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:hypothetical protein vc0802; PDBTitle: crystal structure of a protein of unknown function vc0802 from vibrio2 cholerae, possible transport protein Phyre2 PDB 2bgr chain A domain 2 Region: 9 - 58 Aligned: 50 Modelled: 50 Confidence: 29.2% Identity: 10% Fold: alpha/beta-Hydrolases Superfamily: alpha/beta-Hydrolases Family: DPP6 catalytic domain-like Phyre2 PDB 3jw8 chain A Region: 9 - 58 Aligned: 50 Modelled: 50 Confidence: 28.1% Identity: 12% PDB header:hydrolase Chain: A: PDB Molecule:mgll protein; PDBTitle: crystal structure of human mono-glyceride lipase Phyre2 PDB 3hju chain B Region: 9 - 58 Aligned: 50 Modelled: 50 Confidence: 27.5% Identity: 12% PDB header:hydrolase Chain: B: PDB Molecule:monoglyceride lipase; PDBTitle: crystal structure of human monoglyceride lipase Phyre2 PDB 1zhv chain A Region: 7 - 54 Aligned: 48 Modelled: 48 Confidence: 23.8% Identity: 17% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical protein atu0741; PDBTitle: x-ray crystal structure protein atu0741 from agobacterium tumefaciens.2 northeast structural genomics consortium target atr8. Phyre2 PDB 2v1n chain A Region: 15 - 51 Aligned: 37 Modelled: 37 Confidence: 22.7% Identity: 11% PDB header:nuclear protein Chain: A: PDB Molecule:protein kin homolog; PDBTitle: solution structure of the region 51-160 of human kin172 reveals a winged helix fold Phyre2 PDB 1orv chain A domain 2 Region: 9 - 58 Aligned: 50 Modelled: 50 Confidence: 22.4% Identity: 12% Fold: alpha/beta-Hydrolases Superfamily: alpha/beta-Hydrolases Family: DPP6 catalytic domain-like Phyre2 PDB 3l76 chain B Region: 8 - 67 Aligned: 60 Modelled: 60 Confidence: 19.8% Identity: 25% PDB header:transferase Chain: B: PDB Molecule:aspartokinase; PDBTitle: crystal structure of aspartate kinase from synechocystis Phyre2 1 d3b45a1 2 d2nr9a1 3 c2gqcA_ 4 d1utaa_ 5 c1x60A_ 6 c2y9jt_ 7 c1yj7A_ 8 d1zhva2 9 c2hfvA_ 10 d1zvpa2 11 d2hmfa2 12 d2hfva1 13 c1zvpB_ 14 d2bgra2 15 c3jw8A_ 16 c3hjuB_ 17 c1zhvA_ 18 c2v1nA_ 19 d1orva2 20 c3l76B_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d3b45a1 100.0 100 Fold:Rhomboid-like Superfamily:Rhomboid-like Family:Rhomboid-like 2 d2nr9a1 100.0 36 Fold:Rhomboid-like Superfamily:Rhomboid-like Family:Rhomboid-like 3 c2gqcA_ 98.6 25 PDB header:hydrolase Chain: A: PDB Molecule:rhomboid intramembrane protease; PDBTitle: solution structure of the n-terminal domain of rhomboid intramembrane2 protease from p. aeruginosa 4 d1utaa_ 93.9 19 Fold:Ferredoxin-like Superfamily:Sporulation related repeat Family:Sporulation related repeat 5 c1x60A_ 93.5 15 PDB header:hydrolase Chain: A: PDB Molecule:sporulation-specific n-acetylmuramoyl-l-alanine PDBTitle: solution structure of the peptidoglycan binding domain of2 b. subtilis cell wall lytic enzyme cwlc 6 c2y9jt_ 93.5 4 PDB header:protein transport Chain: T: PDB Molecule:protein prgh; PDBTitle: three-dimensional model of salmonella's needle complex at2 subnanometer resolution 7 c1yj7A_ 92.7 9 PDB header:protein transport Chain: A: PDB Molecule:escj; PDBTitle: crystal structure of enteropathogenic e.coli (epec) type iii secretion2 system protein escj 8 d1zhva2 60.8 16 Fold:Ferredoxin-like Superfamily:ACT-like Family:Atu0741-like 9 c2hfvA_ 53.8 15 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical protein rpa1041; PDBTitle: solution nmr structure of protein rpa1041 from pseudomonas2 aeruginosa. northeast structural genomics consortium3 target pat90. 10 d1zvpa2 51.5 18 Fold:Ferredoxin-like Superfamily:ACT-like Family:VC0802-like 11 d2hmfa2 44.3 18 Fold:Ferredoxin-like Superfamily:ACT-like Family:Aspartokinase allosteric domain-like 12 d2hfva1 37.7 15 Fold:Ferredoxin-like Superfamily:GlnB-like Family:RPA1041-like 13 c1zvpB_ 30.6 18 PDB header:structural genomics, unknown function Chain: B: PDB Molecule:hypothetical protein vc0802; PDBTitle: crystal structure of a protein of unknown function vc0802 from vibrio2 cholerae, possible transport protein 14 d2bgra2 29.2 10 Fold:alpha/beta-Hydrolases Superfamily:alpha/beta-Hydrolases Family:DPP6 catalytic domain-like 15 c3jw8A_ 28.1 12 PDB header:hydrolase Chain: A: PDB Molecule:mgll protein; PDBTitle: crystal structure of human mono-glyceride lipase 16 c3hjuB_ 27.5 12 PDB header:hydrolase Chain: B: PDB Molecule:monoglyceride lipase; PDBTitle: crystal structure of human monoglyceride lipase 17 c1zhvA_ 23.8 17 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical protein atu0741; PDBTitle: x-ray crystal structure protein atu0741 from agobacterium tumefaciens.2 northeast structural genomics consortium target atr8. 18 c2v1nA_ 22.7 11 PDB header:nuclear protein Chain: A: PDB Molecule:protein kin homolog; PDBTitle: solution structure of the region 51-160 of human kin172 reveals a winged helix fold 19 d1orva2 22.4 12 Fold:alpha/beta-Hydrolases Superfamily:alpha/beta-Hydrolases Family:DPP6 catalytic domain-like 20 c3l76B_ 19.8 25 PDB header:transferase Chain: B: PDB Molecule:aspartokinase; PDBTitle: crystal structure of aspartate kinase from synechocystis 21 c2wirB_ not modelled 19.2 9 PDB header:hydrolase Chain: B: PDB Molecule:alpha/beta hydrolase fold-3 domain protein; PDBTitle: hyperthermophilic esterase from the archeon pyrobaculum2 calidifontis 22 d1u4na_ not modelled 19.2 15 Fold:alpha/beta-Hydrolases Superfamily:alpha/beta-Hydrolases Family:Carboxylesterase 23 c2dtjA_ not modelled 18.2 16 PDB header:transferase Chain: A: PDB Molecule:aspartokinase; PDBTitle: crystal structure of regulatory subunit of aspartate kinase2 from corynebacterium glutamicum 24 c3qwuA_ not modelled 17.6 10 PDB header:ligase Chain: A: PDB Molecule:dna ligase; PDBTitle: putative atp-dependent dna ligase from aquifex aeolicus. 25 d2cdqa3 not modelled 15.8 19 Fold:Ferredoxin-like Superfamily:ACT-like Family:Aspartokinase allosteric domain-like 26 d1sbpa_ not modelled 15.1 27 Fold:Periplasmic binding protein-like II Superfamily:Periplasmic binding protein-like II Family:Phosphate binding protein-like 27 c3d7rB_ not modelled 14.9 8 PDB header:hydrolase Chain: B: PDB Molecule:esterase; PDBTitle: crystal structure of a putative esterase from staphylococcus aureus 28 d1xfda2 not modelled 14.2 6 Fold:alpha/beta-Hydrolases Superfamily:alpha/beta-Hydrolases Family:DPP6 catalytic domain-like 29 c1yq3C_ not modelled 12.8 10 PDB header:oxidoreductase Chain: C: PDB Molecule:succinate dehydrogenase cytochrome b, large subunit; PDBTitle: avian respiratory complex ii with oxaloacetate and ubiquinone 30 c2voyG_ not modelled 12.4 23 PDB header:hydrolase Chain: G: PDB Molecule:sarcoplasmic/endoplasmic reticulum calcium PDBTitle: cryoem model of copa, the copper transporting atpase from2 archaeoglobus fulgidus 31 d2hu7a2 not modelled 12.0 8 Fold:alpha/beta-Hydrolases Superfamily:alpha/beta-Hydrolases Family:Acylamino-acid-releasing enzyme, C-terminal donain 32 c3aikB_ not modelled 11.2 12 PDB header:hydrolase Chain: B: PDB Molecule:303aa long hypothetical esterase; PDBTitle: crystal structure of a hsl-like carboxylesterase from sulfolobus2 tokodaii 33 d2boaa2 not modelled 11.1 22 Fold:Ferredoxin-like Superfamily:Protease propeptides/inhibitors Family:Pancreatic carboxypeptidase, activation domain 34 c3gr0D_ not modelled 10.7 11 PDB header:membrane protein Chain: D: PDB Molecule:protein prgh; PDBTitle: periplasmic domain of the t3ss inner membrane protein prgh from2 s.typhimurium (fragment 170-362) 35 d1jqga2 not modelled 10.6 12 Fold:Ferredoxin-like Superfamily:Protease propeptides/inhibitors Family:Pancreatic carboxypeptidase, activation domain 36 c2re1A_ not modelled 10.0 22 PDB header:transferase Chain: A: PDB Molecule:aspartokinase, alpha and beta subunits; PDBTitle: crystal structure of aspartokinase alpha and beta subunits 37 c1nauA_ not modelled 8.8 36 PDB header:hormone/growth factor Chain: A: PDB Molecule:glucagon; PDBTitle: nmr solution structure of the glucagon antagonist [deshis1,2 desphe6, glu9]glucagon amide in the presence of3 perdeuterated dodecylphosphocholine micelles 38 d1tc3c_ not modelled 8.5 27 Fold:DNA/RNA-binding 3-helical bundle Superfamily:Homeodomain-like Family:Recombinase DNA-binding domain 39 c3fakA_ not modelled 8.2 8 PDB header:hydrolase Chain: A: PDB Molecule:esterase/lipase; PDBTitle: structural and functional analysis of a hormone-sensitive2 lipase like este5 from a metagenome library 40 c2ecfA_ not modelled 8.1 10 PDB header:hydrolase Chain: A: PDB Molecule:dipeptidyl peptidase iv; PDBTitle: crystal structure of dipeptidyl aminopeptidase iv from2 stenotrophomonas maltophilia 41 c3ga7A_ not modelled 7.7 9 PDB header:hydrolase Chain: A: PDB Molecule:acetyl esterase; PDBTitle: 1.55 angstrom crystal structure of an acetyl esterase from salmonella2 typhimurium 42 d1udxa3 not modelled 7.6 20 Fold:Obg GTP-binding protein C-terminal domain Superfamily:Obg GTP-binding protein C-terminal domain Family:Obg GTP-binding protein C-terminal domain 43 d1o6xa_ not modelled 7.5 21 Fold:Ferredoxin-like Superfamily:Protease propeptides/inhibitors Family:Pancreatic carboxypeptidase, activation domain 44 c2k9yB_ not modelled 7.2 40 PDB header:transferase Chain: B: PDB Molecule:ephrin type-a receptor 2; PDBTitle: epha2 dimeric structure in the lipidic bicelle at ph 5.0 45 c2k9yA_ not modelled 7.2 40 PDB header:transferase Chain: A: PDB Molecule:ephrin type-a receptor 2; PDBTitle: epha2 dimeric structure in the lipidic bicelle at ph 5.0 46 c1bh0A_ not modelled 6.4 33 PDB header:synthetic hormone Chain: A: PDB Molecule:glucagon; PDBTitle: structure of a glucagon analog 47 c1jrjA_ not modelled 6.2 7 PDB header:hormone/growth factor Chain: A: PDB Molecule:exendin-4; PDBTitle: solution structure of exendin-4 in 30-vol% trifluoroethanol 48 d1h97a_ not modelled 6.2 5 Fold:Globin-like Superfamily:Globin-like Family:Globins 49 d2hmfa3 not modelled 5.8 10 Fold:Ferredoxin-like Superfamily:ACT-like Family:Aspartokinase allosteric domain-like 50 d2c0ra1 not modelled 5.6 12 Fold:PLP-dependent transferase-like Superfamily:PLP-dependent transferases Family:GABA-aminotransferase-like 51 d1lzla_ not modelled 5.6 15 Fold:alpha/beta-Hydrolases Superfamily:alpha/beta-Hydrolases Family:Carboxylesterase 52 c1d0rA_ not modelled 5.4 17 PDB header:hormone/growth factor Chain: A: PDB Molecule:glucagon-like peptide-1-(7-36)-amide; PDBTitle: solution structure of glucagon-like peptide-1-(7-36)-amide2 in trifluoroethanol/water 53 c2zhoB_ not modelled 5.3 13 PDB header:transferase Chain: B: PDB Molecule:aspartokinase; PDBTitle: crystal structure of the regulatory subunit of aspartate2 kinase from thermus thermophilus (ligand free form) 54 c3ofeB_ not modelled 5.3 18 PDB header:chaperone Chain: B: PDB Molecule:ldlr chaperone boca; PDBTitle: structured domain of drosophila melanogaster boca p41 2 2 crystal form

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0