Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	K	R	V	L	T	A	L	A	A	T	L	P	F	A	A	N	A	A	D	A	I	S	G	A	V	E	R	Q	P	T	N	W	Q	A	I	I	M	F	L	I	F	V	V	F	T	L	G	I	T	Y	W	A	S	K	R	V	R	S	R
Secondary structure
SS confidence
Disorder		?												?	?	?	?	?	?	?		?	?	?	?	?	?	?	?	?	?																									?	?				?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		S	D	Y	Y	T	A	G	G	N	I	T	G	F	Q	N	G	L	A	I	A	G	D	Y	M	S	A	A	S	F	L	G	I	S	A	L	V	F	T	S	G	Y	D	G	L	I	Y	S	L	G	F	L	V	G	W	P	I	I	L	F	L
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		I	A	E	R	L	R	N	L	G	R	Y	T	F	A	D	V	A	S	Y	R	L	K	Q	G	P	I	R	I	L	S	A	C	G	S	L	V	V	V	A	L	Y	L	I	A	Q	M	V	G	A	G	K	L	I	E	L	L	F	G	L	N
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		Y	H	I	A	V	V	L	V	G	V	L	M	M	M	Y	V	L	F	G	G	M	L	A	T	T	W	V	Q	I	I	K	A	V	L	L	L	F	G	A	S	F	M	A	F	M	V	M	K	H	V	G	F	S	F	N	N	L	F	S	E
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		A	M	A	V	H	P	K	G	V	D	I	M	K	P	G	G	L	V	K	D	P	I	S	A	L	S	L	G	L	G	L	M	F	G	T	A	G	L	P	H	I	L	M	R	F	F	T	V	S	D	A	R	E	A	R	K	S	V	F	Y
Secondary structure
SS confidence
Disorder							?	?							?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		A	T	G	F	M	G	Y	F	Y	I	L	T	F	I	I	G	F	G	A	I	M	L	V	G	A	N	P	E	Y	K	D	A	A	G	H	L	I	G	G	N	N	M	A	A	V	H	L	A	N	A	V	G	G	N	L	F	L	G	F	I
Secondary structure
SS confidence
Disorder																														?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		S	A	V	A	F	A	T	I	L	A	V	V	A	G	L	T	L	A	G	A	S	A	V	S	H	D	L	Y	A	N	V	F	K	K	G	A	T	E	R	E	E	L	R	V	S	K	I	T	V	L	I	L	G	V	I	A	I	I	L	G
Secondary structure
SS confidence
Disorder																																			?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		V	L	F	E	N	Q	N	I	A	F	M	V	G	L	A	F	A	I	A	A	S	C	N	F	P	I	I	L	L	S	M	Y	W	S	K	L	T	T	R	G	A	M	M	G	G	W	L	G	L	I	T	A	V	V	L	M	I	L	G	P
Secondary structure
SS confidence
Disorder																																																													?
Disorder confidence
		.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.	510	.	.	.	.	.	.	.	.	.	520	.	.	.	.	.	.	.	.	.	530	.	.	.	.	.	.	.	.	.	540
Sequence		T	I	W	V	Q	I	L	G	H	E	K	A	I	F	P	Y	E	Y	P	A	L	F	S	I	T	V	A	F	L	G	I	W	F	F	S	A	T	D	N	S	A	E	G	A	R	E	R	E	L	F	R	A	Q	F	I	R	S	Q	T	G
Secondary structure
SS confidence
Disorder		?	?	?	?	?																														?	?	?		?	?	?	?													?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.
Sequence		F	G	V	E	Q	G	R	A	H
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2xq2 chain A Region: 33 - 549 Aligned: 485 Modelled: 485 Confidence: 100.0% Identity: 16% PDB header:transport protein Chain: A: PDB Molecule:sodium/glucose cotransporter; PDBTitle: structure of the k294a mutant of vsglt Phyre2 PDB 3dh4 chain A Region: 54 - 525 Aligned: 460 Modelled: 448 Confidence: 100.0% Identity: 14% PDB header:transport protein Chain: A: PDB Molecule:sodium/glucose cotransporter; PDBTitle: crystal structure of sodium/sugar symporter with bound galactose from2 vibrio parahaemolyticus Phyre2 PDB 2jln chain A Region: 66 - 540 Aligned: 431 Modelled: 431 Confidence: 99.4% Identity: 12% PDB header:membrane protein Chain: A: PDB Molecule:mhp1; PDBTitle: structure of mhp1, a nucleobase-cation-symport-1 family2 transporter Phyre2 PDB 3gia chain A Region: 67 - 526 Aligned: 423 Modelled: 423 Confidence: 99.3% Identity: 9% PDB header:transport protein Chain: A: PDB Molecule:uncharacterized protein mj0609; PDBTitle: crystal structure of apct transporter Phyre2 PDB 3lrc chain C Region: 68 - 522 Aligned: 400 Modelled: 400 Confidence: 98.7% Identity: 12% PDB header:transport protein Chain: C: PDB Molecule:arginine/agmatine antiporter; PDBTitle: structure of e. coli adic (p1) Phyre2 PDB 2w8a chain C Region: 4 - 452 Aligned: 423 Modelled: 447 Confidence: 97.0% Identity: 13% PDB header:membrane protein Chain: C: PDB Molecule:glycine betaine transporter betp; PDBTitle: crystal structure of the sodium-coupled glycine betaine2 symporter betp from corynebacterium glutamicum with bound3 substrate Phyre2 PDB 3hfx chain A Region: 32 - 452 Aligned: 408 Modelled: 408 Confidence: 96.8% Identity: 12% PDB header:transport protein Chain: A: PDB Molecule:l-carnitine/gamma-butyrobetaine antiporter; PDBTitle: crystal structure of carnitine transporter Phyre2 PDB 2a65 chain A domain 1 Region: 71 - 455 Aligned: 370 Modelled: 370 Confidence: 91.0% Identity: 14% Fold: SNF-like Superfamily: SNF-like Family: SNF-like Phyre2 PDB 3rlb chain A Region: 410 - 523 Aligned: 112 Modelled: 114 Confidence: 88.7% Identity: 17% PDB header:thiamine-binding protein Chain: A: PDB Molecule:thit; PDBTitle: crystal structure at 2.0 a of the s-component for thiamin from an ecf-2 type abc transporter Phyre2 PDB 3mk7 chain F Region: 33 - 94 Aligned: 62 Modelled: 62 Confidence: 49.1% Identity: 11% PDB header:oxidoreductase Chain: F: PDB Molecule:cytochrome c oxidase, cbb3-type, subunit p; PDBTitle: the structure of cbb3 cytochrome oxidase Phyre2 PDB 3ehb chain B domain 2 Region: 33 - 70 Aligned: 38 Modelled: 38 Confidence: 9.8% Identity: 11% Fold: Transmembrane helix hairpin Superfamily: Cytochrome c oxidase subunit II-like, transmembrane region Family: Cytochrome c oxidase subunit II-like, transmembrane region Phyre2 PDB 2ky5 chain A Region: 133 - 144 Aligned: 11 Modelled: 12 Confidence: 8.0% Identity: 18% PDB header:cell adhesion Chain: A: PDB Molecule:platelet endothelial cell adhesion molecule; PDBTitle: solution structure of the pecam-1 cytoplasmic tail with dpc Phyre2 PDB 3rko chain K Region: 24 - 61 Aligned: 38 Modelled: 38 Confidence: 7.9% Identity: 16% PDB header:oxidoreductase Chain: K: PDB Molecule:nadh-quinone oxidoreductase subunit k; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 e. coli at 3.0 angstrom resolution Phyre2 PDB 2ks1 chain B Region: 39 - 56 Aligned: 18 Modelled: 18 Confidence: 7.8% Identity: 33% PDB header:transferase Chain: B: PDB Molecule:epidermal growth factor receptor; PDBTitle: heterodimeric association of transmembrane domains of erbb1 and erbb22 receptors enabling kinase activation Phyre2 PDB 2k9y chain A Region: 40 - 58 Aligned: 19 Modelled: 19 Confidence: 7.3% Identity: 11% PDB header:transferase Chain: A: PDB Molecule:ephrin type-a receptor 2; PDBTitle: epha2 dimeric structure in the lipidic bicelle at ph 5.0 Phyre2 PDB 2k9y chain B Region: 40 - 58 Aligned: 19 Modelled: 19 Confidence: 7.3% Identity: 11% PDB header:transferase Chain: B: PDB Molecule:ephrin type-a receptor 2; PDBTitle: epha2 dimeric structure in the lipidic bicelle at ph 5.0 Phyre2 PDB 3dtu chain B domain 2 Region: 33 - 70 Aligned: 38 Modelled: 38 Confidence: 6.3% Identity: 5% Fold: Transmembrane helix hairpin Superfamily: Cytochrome c oxidase subunit II-like, transmembrane region Family: Cytochrome c oxidase subunit II-like, transmembrane region Phyre2 PDB 1si2 chain A Region: 131 - 142 Aligned: 12 Modelled: 12 Confidence: 5.7% Identity: 17% Fold: SH3-like barrel Superfamily: PAZ domain Family: PAZ domain Phyre2 PDB 2k21 chain A Region: 6 - 62 Aligned: 57 Modelled: 57 Confidence: 5.6% Identity: 14% PDB header:membrane protein Chain: A: PDB Molecule:potassium voltage-gated channel subfamily e PDBTitle: nmr structure of human kcne1 in lmpg micelles at ph 6.0 and2 40 degree c Phyre2 1 c2xq2A_ 2 c3dh4A_ 3 c2jlnA_ 4 c3giaA_ 5 c3lrcC_ 6 c2w8aC_ 7 c3hfxA_ 8 d2a65a1 9 c3rlbA_ 10 c3mk7F_ 11 d3ehbb2 12 c2ky5A_ 13 c3rkoK_ 14 c2ks1B_ 15 c2k9yA_ 16 c2k9yB_ 17 d3dtub2 18 d1si2a_ 19 c2k21A_

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c2xq2A_ 100.0 16 PDB header:transport protein Chain: A: PDB Molecule:sodium/glucose cotransporter; PDBTitle: structure of the k294a mutant of vsglt 2 c3dh4A_ 100.0 14 PDB header:transport protein Chain: A: PDB Molecule:sodium/glucose cotransporter; PDBTitle: crystal structure of sodium/sugar symporter with bound galactose from2 vibrio parahaemolyticus 3 c2jlnA_ 99.4 12 PDB header:membrane protein Chain: A: PDB Molecule:mhp1; PDBTitle: structure of mhp1, a nucleobase-cation-symport-1 family2 transporter 4 c3giaA_ 99.3 9 PDB header:transport protein Chain: A: PDB Molecule:uncharacterized protein mj0609; PDBTitle: crystal structure of apct transporter 5 c3lrcC_ 98.7 12 PDB header:transport protein Chain: C: PDB Molecule:arginine/agmatine antiporter; PDBTitle: structure of e. coli adic (p1) 6 c2w8aC_ 97.0 13 PDB header:membrane protein Chain: C: PDB Molecule:glycine betaine transporter betp; PDBTitle: crystal structure of the sodium-coupled glycine betaine2 symporter betp from corynebacterium glutamicum with bound3 substrate 7 c3hfxA_ 96.8 12 PDB header:transport protein Chain: A: PDB Molecule:l-carnitine/gamma-butyrobetaine antiporter; PDBTitle: crystal structure of carnitine transporter 8 d2a65a1 91.0 14 Fold:SNF-like Superfamily:SNF-like Family:SNF-like 9 c3rlbA_ 88.7 17 PDB header:thiamine-binding protein Chain: A: PDB Molecule:thit; PDBTitle: crystal structure at 2.0 a of the s-component for thiamin from an ecf-2 type abc transporter 10 c3mk7F_ 49.1 11 PDB header:oxidoreductase Chain: F: PDB Molecule:cytochrome c oxidase, cbb3-type, subunit p; PDBTitle: the structure of cbb3 cytochrome oxidase 11 d3ehbb2 9.8 11 Fold:Transmembrane helix hairpin Superfamily:Cytochrome c oxidase subunit II-like, transmembrane region Family:Cytochrome c oxidase subunit II-like, transmembrane region 12 c2ky5A_ 8.0 18 PDB header:cell adhesion Chain: A: PDB Molecule:platelet endothelial cell adhesion molecule; PDBTitle: solution structure of the pecam-1 cytoplasmic tail with dpc 13 c3rkoK_ 7.9 16 PDB header:oxidoreductase Chain: K: PDB Molecule:nadh-quinone oxidoreductase subunit k; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 e. coli at 3.0 angstrom resolution 14 c2ks1B_ 7.8 33 PDB header:transferase Chain: B: PDB Molecule:epidermal growth factor receptor; PDBTitle: heterodimeric association of transmembrane domains of erbb1 and erbb22 receptors enabling kinase activation 15 c2k9yA_ 7.3 11 PDB header:transferase Chain: A: PDB Molecule:ephrin type-a receptor 2; PDBTitle: epha2 dimeric structure in the lipidic bicelle at ph 5.0 16 c2k9yB_ 7.3 11 PDB header:transferase Chain: B: PDB Molecule:ephrin type-a receptor 2; PDBTitle: epha2 dimeric structure in the lipidic bicelle at ph 5.0 17 d3dtub2 6.3 5 Fold:Transmembrane helix hairpin Superfamily:Cytochrome c oxidase subunit II-like, transmembrane region Family:Cytochrome c oxidase subunit II-like, transmembrane region 18 d1si2a_ 5.7 17 Fold:SH3-like barrel Superfamily:PAZ domain Family:PAZ domain 19 c2k21A_ 5.6 14 PDB header:membrane protein Chain: A: PDB Molecule:potassium voltage-gated channel subfamily e PDBTitle: nmr structure of human kcne1 in lmpg micelles at ph 6.0 and2 40 degree c

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

---

Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0