Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	S	S	D	A	D	A	H	K	V	G	L	I	P	V	T	L	M	V	S	G	N	I	M	G	S	G	V	F	L	L	P	A	N	L	A	S	T	G	G	I	A	I	Y	G	W	L	V	T	I	I	G	A	L	G	L	S	M	V	Y
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		A	K	M	S	F	L	D	P	S	P	G	G	S	Y	A	Y	A	R	R	C	F	G	P	F	L	G	Y	Q	T	N	V	L	Y	W	L	A	C	W	I	G	N	I	A	M	V	V	I	G	V	G	Y	L	S	Y	F	F	P	I	L	K
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		D	P	L	V	L	T	I	T	C	V	V	V	L	W	I	F	V	L	L	N	I	V	G	P	K	M	I	T	R	V	Q	A	V	A	T	V	L	A	L	I	P	I	V	G	I	A	V	F	G	W	F	W	F	R	G	E	T	Y	M	A
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		A	W	N	V	S	G	L	G	T	F	G	A	I	Q	S	T	L	N	V	T	L	W	S	F	I	G	V	E	S	A	S	V	A	A	G	V	V	K	N	P	K	R	N	V	P	I	A	T	I	G	G	V	L	I	A	A	V	C	Y	V
Secondary structure
SS confidence
Disorder					?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		L	S	T	T	A	I	M	G	M	I	P	N	A	A	L	R	V	S	A	S	P	F	G	D	A	A	R	M	A	L	G	D	T	A	G	A	I	V	S	F	C	A	A	A	G	C	L	G	S	L	G	G	W	T	L	L	A	G	Q	T
Secondary structure
SS confidence
Disorder																?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		A	K	A	A	A	D	D	G	L	F	P	P	I	F	A	R	V	N	K	A	G	T	P	V	A	G	L	I	I	V	G	I	L	M	T	I	F	Q	L	S	S	I	S	P	N	A	T	K	E	F	G	L	V	S	S	V	S	V	I	F
Secondary structure
SS confidence
Disorder																																											?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		T	L	V	P	Y	L	Y	T	C	A	A	L	L	L	L	G	H	G	H	F	G	K	A	R	P	A	Y	L	A	V	T	T	I	A	F	L	Y	C	I	W	A	V	V	G	S	G	A	K	E	V	M	W	S	F	V	T	L	M	V	I
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.
Sequence		T	A	M	Y	A	L	N	Y	N	R	L	H	K	N	P	Y	P	L	D	A	P	I	S	K	D
Secondary structure
SS confidence
Disorder												?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 3lrc chain C Region: 6 - 435 Aligned: 410 Modelled: 410 Confidence: 100.0% Identity: 100% PDB header:transport protein Chain: C: PDB Molecule:arginine/agmatine antiporter; PDBTitle: structure of e. coli adic (p1) Phyre2 PDB 3gia chain A Region: 6 - 443 Aligned: 427 Modelled: 427 Confidence: 100.0% Identity: 18% PDB header:transport protein Chain: A: PDB Molecule:uncharacterized protein mj0609; PDBTitle: crystal structure of apct transporter Phyre2 PDB 2jln chain A Region: 3 - 445 Aligned: 430 Modelled: 430 Confidence: 100.0% Identity: 13% PDB header:membrane protein Chain: A: PDB Molecule:mhp1; PDBTitle: structure of mhp1, a nucleobase-cation-symport-1 family2 transporter Phyre2 PDB 2xq2 chain A Region: 8 - 444 Aligned: 429 Modelled: 429 Confidence: 99.6% Identity: 12% PDB header:transport protein Chain: A: PDB Molecule:sodium/glucose cotransporter; PDBTitle: structure of the k294a mutant of vsglt Phyre2 PDB 3dh4 chain A Region: 8 - 445 Aligned: 434 Modelled: 434 Confidence: 99.5% Identity: 11% PDB header:transport protein Chain: A: PDB Molecule:sodium/glucose cotransporter; PDBTitle: crystal structure of sodium/sugar symporter with bound galactose from2 vibrio parahaemolyticus Phyre2 PDB 2a65 chain A domain 1 Region: 11 - 434 Aligned: 414 Modelled: 414 Confidence: 97.6% Identity: 14% Fold: SNF-like Superfamily: SNF-like Family: SNF-like Phyre2 PDB 2w8a chain C Region: 3 - 387 Aligned: 375 Modelled: 358 Confidence: 97.5% Identity: 13% PDB header:membrane protein Chain: C: PDB Molecule:glycine betaine transporter betp; PDBTitle: crystal structure of the sodium-coupled glycine betaine2 symporter betp from corynebacterium glutamicum with bound3 substrate Phyre2 PDB 3hfx chain A Region: 32 - 382 Aligned: 342 Modelled: 342 Confidence: 85.1% Identity: 11% PDB header:transport protein Chain: A: PDB Molecule:l-carnitine/gamma-butyrobetaine antiporter; PDBTitle: crystal structure of carnitine transporter Phyre2 PDB 2knc chain A Region: 403 - 445 Aligned: 43 Modelled: 43 Confidence: 55.1% Identity: 7% PDB header:cell adhesion Chain: A: PDB Molecule:integrin alpha-iib; PDBTitle: platelet integrin alfaiib-beta3 transmembrane-cytoplasmic2 heterocomplex Phyre2 PDB 1fft chain B domain 2 Region: 417 - 444 Aligned: 28 Modelled: 28 Confidence: 23.3% Identity: 11% Fold: Transmembrane helix hairpin Superfamily: Cytochrome c oxidase subunit II-like, transmembrane region Family: Cytochrome c oxidase subunit II-like, transmembrane region Phyre2 PDB 3qnq chain D Region: 395 - 445 Aligned: 51 Modelled: 51 Confidence: 19.0% Identity: 12% PDB header:membrane protein, transport protein Chain: D: PDB Molecule:pts system, cellobiose-specific iic component; PDBTitle: crystal structure of the transporter chbc, the iic component from the2 n,n'-diacetylchitobiose-specific phosphotransferase system Phyre2 PDB 3rko chain F Region: 320 - 436 Aligned: 117 Modelled: 117 Confidence: 13.4% Identity: 14% PDB header:oxidoreductase Chain: F: PDB Molecule:nadh-quinone oxidoreductase subunit j; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 e. coli at 3.0 angstrom resolution Phyre2 PDB 3dtu chain B domain 2 Region: 409 - 441 Aligned: 33 Modelled: 33 Confidence: 12.5% Identity: 12% Fold: Transmembrane helix hairpin Superfamily: Cytochrome c oxidase subunit II-like, transmembrane region Family: Cytochrome c oxidase subunit II-like, transmembrane region Phyre2 PDB 1m57 chain H Region: 409 - 441 Aligned: 33 Modelled: 33 Confidence: 8.2% Identity: 12% PDB header:oxidoreductase Chain: H: PDB Molecule:cytochrome c oxidase; PDBTitle: structure of cytochrome c oxidase from rhodobacter2 sphaeroides (eq(i-286) mutant)) Phyre2 PDB 2iub chain A domain 2 Region: 388 - 431 Aligned: 44 Modelled: 44 Confidence: 6.8% Identity: 11% Fold: Transmembrane helix hairpin Superfamily: Magnesium transport protein CorA, transmembrane region Family: Magnesium transport protein CorA, transmembrane region Phyre2 PDB 1ar1 chain B Region: 409 - 444 Aligned: 36 Modelled: 36 Confidence: 6.3% Identity: 8% PDB header:complex (oxidoreductase/antibody) Chain: B: PDB Molecule:cytochrome c oxidase; PDBTitle: structure at 2.7 angstrom resolution of the paracoccus2 denitrificans two-subunit cytochrome c oxidase complexed3 with an antibody fv fragment Phyre2 1 c3lrcC_ 2 c3giaA_ 3 c2jlnA_ 4 c2xq2A_ 5 c3dh4A_ 6 d2a65a1 7 c2w8aC_ 8 c3hfxA_ 9 c2kncA_ 10 d1fftb2 11 c3qnqD_ 12 c3rkoF_ 13 d3dtub2 14 c1m57H_ 15 d2iuba2 16 c1ar1B_

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c3lrcC_ 100.0 100 PDB header:transport protein Chain: C: PDB Molecule:arginine/agmatine antiporter; PDBTitle: structure of e. coli adic (p1) 2 c3giaA_ 100.0 18 PDB header:transport protein Chain: A: PDB Molecule:uncharacterized protein mj0609; PDBTitle: crystal structure of apct transporter 3 c2jlnA_ 100.0 13 PDB header:membrane protein Chain: A: PDB Molecule:mhp1; PDBTitle: structure of mhp1, a nucleobase-cation-symport-1 family2 transporter 4 c2xq2A_ 99.6 12 PDB header:transport protein Chain: A: PDB Molecule:sodium/glucose cotransporter; PDBTitle: structure of the k294a mutant of vsglt 5 c3dh4A_ 99.5 11 PDB header:transport protein Chain: A: PDB Molecule:sodium/glucose cotransporter; PDBTitle: crystal structure of sodium/sugar symporter with bound galactose from2 vibrio parahaemolyticus 6 d2a65a1 97.6 14 Fold:SNF-like Superfamily:SNF-like Family:SNF-like 7 c2w8aC_ 97.5 13 PDB header:membrane protein Chain: C: PDB Molecule:glycine betaine transporter betp; PDBTitle: crystal structure of the sodium-coupled glycine betaine2 symporter betp from corynebacterium glutamicum with bound3 substrate 8 c3hfxA_ 85.1 11 PDB header:transport protein Chain: A: PDB Molecule:l-carnitine/gamma-butyrobetaine antiporter; PDBTitle: crystal structure of carnitine transporter 9 c2kncA_ 55.1 7 PDB header:cell adhesion Chain: A: PDB Molecule:integrin alpha-iib; PDBTitle: platelet integrin alfaiib-beta3 transmembrane-cytoplasmic2 heterocomplex 10 d1fftb2 23.3 11 Fold:Transmembrane helix hairpin Superfamily:Cytochrome c oxidase subunit II-like, transmembrane region Family:Cytochrome c oxidase subunit II-like, transmembrane region 11 c3qnqD_ 19.0 12 PDB header:membrane protein, transport protein Chain: D: PDB Molecule:pts system, cellobiose-specific iic component; PDBTitle: crystal structure of the transporter chbc, the iic component from the2 n,n'-diacetylchitobiose-specific phosphotransferase system 12 c3rkoF_ 13.4 14 PDB header:oxidoreductase Chain: F: PDB Molecule:nadh-quinone oxidoreductase subunit j; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 e. coli at 3.0 angstrom resolution 13 d3dtub2 12.5 12 Fold:Transmembrane helix hairpin Superfamily:Cytochrome c oxidase subunit II-like, transmembrane region Family:Cytochrome c oxidase subunit II-like, transmembrane region 14 c1m57H_ 8.2 12 PDB header:oxidoreductase Chain: H: PDB Molecule:cytochrome c oxidase; PDBTitle: structure of cytochrome c oxidase from rhodobacter2 sphaeroides (eq(i-286) mutant)) 15 d2iuba2 6.8 11 Fold:Transmembrane helix hairpin Superfamily:Magnesium transport protein CorA, transmembrane region Family:Magnesium transport protein CorA, transmembrane region 16 c1ar1B_ 6.3 8 PDB header:complex (oxidoreductase/antibody) Chain: B: PDB Molecule:cytochrome c oxidase; PDBTitle: structure at 2.7 angstrom resolution of the paracoccus2 denitrificans two-subunit cytochrome c oxidase complexed3 with an antibody fv fragment

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0