Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	N	K	A	I	K	V	S	L	Y	I	S	F	V	L	I	I	C	A	L	S	K	N	I	M	M	L	N	T	S	D	F	G	R	A	I	K	P	L	I	E	D	I	P	A	F	T	Y	D	L	P	L	L	Y	K	L	K	G	H	I
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?																?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		D	S	I	D	S	Y	E	Y	I	S	S	Y	S	Y	I	L	Y	T	Y	V	L	F	I	S	I	F	T	E	Y	L	D	A	R	V	L	S	L	F	L	K	V	I	Y	I	Y	S	L	Y	A	I	F	T	S	Y	I	K	T	E	R	Y
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		V	T	L	F	T	F	F	I	L	A	F	L	M	C	S	S	S	T	L	S	M	F	A	S	F	Y	Q	E	Q	I	V	I	I	F	L	P	F	L	V	Y	S	L	T	C	K	N	N	K	S	M	L	L	L	F	F	S	L	L	I	I
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		S	T	A	K	N	Q	F	I	L	T	P	L	I	V	Y	S	Y	Y	I	F	F	D	R	H	K	L	I	I	K	S	V	I	C	V	V	C	L	L	A	S	I	F	A	I	S	Y	S	K	G	V	V	E	L	N	K	Y	H	A	T	Y
Secondary structure
SS confidence
Disorder			?		?	?		?			?																																				?	?					?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		F	G	S	Y	L	Y	M	K	N	N	G	Y	K	M	P	S	Y	V	D	D	K	C	V	G	L	D	A	W	G	N	K	F	D	I	S	F	G	A	T	P	T	E	V	G	T	E	C	F	E	S	H	K	D	E	T	F	S	N	A	L
Secondary structure
SS confidence
Disorder																																							?	?	?	?	?	?										?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		F	L	L	V	S	K	P	S	T	I	F	K	L	P	F	D	D	G	V	M	S	Q	Y	K	E	N	Y	F	H	V	Y	K	K	L	H	V	I	Y	G	E	S	N	I	L	T	T	I	T	N	I	K	D	N	I	F	K	N	I	R	F
Secondary structure
SS confidence
Disorder																		?	?	?	?	?	?	?	?	?	?	?	?	?	?		?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		I	S	L	L	L	F	F	I	A	S	I	F	I	R	N	N	K	I	K	A	S	L	F	V	V	S	L	F	G	I	S	Q	F	Y	V	S	F	F	G	E	G	Y	R	D	L	S	K	H	L	F	G	M	Y	F	S	F	D	L	C	L
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.
Sequence		Y	I	T	V	V	F	L	I	Y	K	I	I	Q	R	N	Q	D	N	S	D	V	K	H
Secondary structure
SS confidence
Disorder														?		?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 3rce chain A Region: 2 - 230 Aligned: 221 Modelled: 229 Confidence: 96.5% Identity: 14% PDB header:transferase/peptide Chain: A: PDB Molecule:oligosaccharide transferase to n-glycosylate proteins; PDBTitle: bacterial oligosaccharyltransferase pglb Phyre2 PDB 1xap chain A Region: 251 - 314 Aligned: 62 Modelled: 64 Confidence: 23.7% Identity: 13% Fold: Nuclear receptor ligand-binding domain Superfamily: Nuclear receptor ligand-binding domain Family: Nuclear receptor ligand-binding domain Phyre2 PDB 2pqf chain F Region: 14 - 37 Aligned: 24 Modelled: 24 Confidence: 19.9% Identity: 17% PDB header:transferase Chain: F: PDB Molecule:poly [adp-ribose] polymerase 12; PDBTitle: human poly(adp-ribose) polymerase 12, catalytic fragment in complex2 with an inhibitor 3-aminobenzoic acid Phyre2 PDB 3d24 chain A domain 1 Region: 252 - 313 Aligned: 59 Modelled: 62 Confidence: 17.0% Identity: 7% Fold: Nuclear receptor ligand-binding domain Superfamily: Nuclear receptor ligand-binding domain Family: Nuclear receptor ligand-binding domain Phyre2 PDB 3d24 chain A Region: 252 - 313 Aligned: 59 Modelled: 62 Confidence: 17.0% Identity: 7% PDB header:transcription Chain: A: PDB Molecule:steroid hormone receptor err1; PDBTitle: crystal structure of ligand-binding domain of estrogen-2 related receptor alpha (erralpha) in complex with the3 peroxisome proliferators-activated receptor coactivator-4 1alpha box3 peptide (pgc-1alpha) Phyre2 PDB 2pd0 chain D Region: 400 - 414 Aligned: 15 Modelled: 15 Confidence: 15.9% Identity: 47% PDB header:structural genomics, unknown function Chain: D: PDB Molecule:hypothetical protein; PDBTitle: protein cgd2_2020 from cryptosporidium parvum Phyre2 PDB 1ef4 chain A Region: 240 - 263 Aligned: 24 Modelled: 24 Confidence: 10.4% Identity: 4% Fold: DNA/RNA-binding 3-helical bundle Superfamily: RNA polymerase subunit RPB10 Family: RNA polymerase subunit RPB10 Phyre2 PDB 2pmz chain N Region: 240 - 263 Aligned: 24 Modelled: 24 Confidence: 10.3% Identity: 8% PDB header:translation, transferase Chain: N: PDB Molecule:dna-directed rna polymerase subunit n; PDBTitle: archaeal rna polymerase from sulfolobus solfataricus Phyre2 PDB 1yvy chain A Region: 12 - 35 Aligned: 24 Modelled: 24 Confidence: 9.9% Identity: 21% PDB header:lyase Chain: A: PDB Molecule:phosphoenolpyruvate carboxykinase [atp]; PDBTitle: crystal strucutre of anaerobiospirillum succiniciproducens2 phosphoenolpyruvate carboxykinase Phyre2 PDB 3pmg chain A domain 2 Region: 13 - 44 Aligned: 32 Modelled: 32 Confidence: 9.2% Identity: 13% Fold: Phosphoglucomutase, first 3 domains Superfamily: Phosphoglucomutase, first 3 domains Family: Phosphoglucomutase, first 3 domains Phyre2 PDB 1tuz chain A Region: 389 - 410 Aligned: 22 Modelled: 22 Confidence: 9.2% Identity: 36% Fold: EF Hand-like Superfamily: EF-hand Family: EF-hand modules in multidomain proteins Phyre2 PDB 3eip chain A Region: 245 - 268 Aligned: 24 Modelled: 24 Confidence: 8.0% Identity: 13% Fold: FKBP-like Superfamily: Colicin E3 immunity protein Family: Colicin E3 immunity protein Phyre2 PDB 1a7j chain A Region: 397 - 419 Aligned: 23 Modelled: 23 Confidence: 7.8% Identity: 13% Fold: P-loop containing nucleoside triphosphate hydrolases Superfamily: P-loop containing nucleoside triphosphate hydrolases Family: Phosphoribulokinase/pantothenate kinase Phyre2 PDB 3do9 chain C Region: 298 - 342 Aligned: 45 Modelled: 45 Confidence: 7.7% Identity: 11% PDB header:structural genomics, unknown function Chain: C: PDB Molecule:upf0302 protein ba_1542/gbaa1542/bas1430; PDBTitle: crystal structure of protein ba1542 from bacillus anthracis2 str.ames Phyre2 PDB 3bge chain A domain 1 Region: 15 - 29 Aligned: 15 Modelled: 15 Confidence: 7.2% Identity: 7% Fold: post-AAA+ oligomerization domain-like Superfamily: post-AAA+ oligomerization domain-like Family: MgsA/YrvN C-terminal domain-like Phyre2 PDB 2p0o chain A Region: 243 - 295 Aligned: 52 Modelled: 53 Confidence: 7.1% Identity: 15% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical protein duf871; PDBTitle: crystal structure of a conserved protein from locus ef_2437 in2 enterococcus faecalis with an unknown function Phyre2 PDB 3rko chain F Region: 90 - 147 Aligned: 58 Modelled: 58 Confidence: 7.1% Identity: 17% PDB header:oxidoreductase Chain: F: PDB Molecule:nadh-quinone oxidoreductase subunit j; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 e. coli at 3.0 angstrom resolution Phyre2 PDB 1twf chain J Region: 234 - 263 Aligned: 30 Modelled: 30 Confidence: 7.0% Identity: 7% Fold: DNA/RNA-binding 3-helical bundle Superfamily: RNA polymerase subunit RPB10 Family: RNA polymerase subunit RPB10 Phyre2 PDB 3let chain B Region: 395 - 411 Aligned: 17 Modelled: 17 Confidence: 6.7% Identity: 35% PDB header:transferase Chain: B: PDB Molecule:adenosine monophosphate-protein transferase vops; PDBTitle: crystal structure of fic domain containing ampylator, vops Phyre2 PDB 2ijo chain A Region: 22 - 32 Aligned: 11 Modelled: 11 Confidence: 6.7% Identity: 9% PDB header:hydrolase/hydrolase inhibitor Chain: A: PDB Molecule:polyprotein; PDBTitle: crystal structure of the west nile virus ns2b-ns3 protease2 complexed with bovine pancreatic trypsin inhibitor Phyre2 1 c3rceA_ 2 d1xapa_ 3 c2pqfF_ 4 d3d24a1 5 c3d24A_ 6 c2pd0D_ 7 d1ef4a_ 8 c2pmzN_ 9 c1yvyA_ 10 d3pmga2 11 d1tuza_ 12 d3eipa_ 13 d1a7ja_ 14 c3do9C_ 15 d3bgea1 16 c2p0oA_ 17 c3rkoF_ 18 d1twfj_ 19 c3letB_ 20 c2ijoA_ 21 22 23 24 25 26 27

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c3rceA_ 96.5 14 PDB header:transferase/peptide Chain: A: PDB Molecule:oligosaccharide transferase to n-glycosylate proteins; PDBTitle: bacterial oligosaccharyltransferase pglb 2 d1xapa_ 23.7 13 Fold:Nuclear receptor ligand-binding domain Superfamily:Nuclear receptor ligand-binding domain Family:Nuclear receptor ligand-binding domain 3 c2pqfF_ 19.9 17 PDB header:transferase Chain: F: PDB Molecule:poly [adp-ribose] polymerase 12; PDBTitle: human poly(adp-ribose) polymerase 12, catalytic fragment in complex2 with an inhibitor 3-aminobenzoic acid 4 d3d24a1 17.0 7 Fold:Nuclear receptor ligand-binding domain Superfamily:Nuclear receptor ligand-binding domain Family:Nuclear receptor ligand-binding domain 5 c3d24A_ 17.0 7 PDB header:transcription Chain: A: PDB Molecule:steroid hormone receptor err1; PDBTitle: crystal structure of ligand-binding domain of estrogen-2 related receptor alpha (erralpha) in complex with the3 peroxisome proliferators-activated receptor coactivator-4 1alpha box3 peptide (pgc-1alpha) 6 c2pd0D_ 15.9 47 PDB header:structural genomics, unknown function Chain: D: PDB Molecule:hypothetical protein; PDBTitle: protein cgd2_2020 from cryptosporidium parvum 7 d1ef4a_ 10.4 4 Fold:DNA/RNA-binding 3-helical bundle Superfamily:RNA polymerase subunit RPB10 Family:RNA polymerase subunit RPB10 8 c2pmzN_ 10.3 8 PDB header:translation, transferase Chain: N: PDB Molecule:dna-directed rna polymerase subunit n; PDBTitle: archaeal rna polymerase from sulfolobus solfataricus 9 c1yvyA_ 9.9 21 PDB header:lyase Chain: A: PDB Molecule:phosphoenolpyruvate carboxykinase [atp]; PDBTitle: crystal strucutre of anaerobiospirillum succiniciproducens2 phosphoenolpyruvate carboxykinase 10 d3pmga2 9.2 13 Fold:Phosphoglucomutase, first 3 domains Superfamily:Phosphoglucomutase, first 3 domains Family:Phosphoglucomutase, first 3 domains 11 d1tuza_ 9.2 36 Fold:EF Hand-like Superfamily:EF-hand Family:EF-hand modules in multidomain proteins 12 d3eipa_ 8.0 13 Fold:FKBP-like Superfamily:Colicin E3 immunity protein Family:Colicin E3 immunity protein 13 d1a7ja_ 7.8 13 Fold:P-loop containing nucleoside triphosphate hydrolases Superfamily:P-loop containing nucleoside triphosphate hydrolases Family:Phosphoribulokinase/pantothenate kinase 14 c3do9C_ 7.7 11 PDB header:structural genomics, unknown function Chain: C: PDB Molecule:upf0302 protein ba_1542/gbaa1542/bas1430; PDBTitle: crystal structure of protein ba1542 from bacillus anthracis2 str.ames 15 d3bgea1 7.2 7 Fold:post-AAA+ oligomerization domain-like Superfamily:post-AAA+ oligomerization domain-like Family:MgsA/YrvN C-terminal domain-like 16 c2p0oA_ 7.1 15 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical protein duf871; PDBTitle: crystal structure of a conserved protein from locus ef_2437 in2 enterococcus faecalis with an unknown function 17 c3rkoF_ 7.1 17 PDB header:oxidoreductase Chain: F: PDB Molecule:nadh-quinone oxidoreductase subunit j; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 e. coli at 3.0 angstrom resolution 18 d1twfj_ 7.0 7 Fold:DNA/RNA-binding 3-helical bundle Superfamily:RNA polymerase subunit RPB10 Family:RNA polymerase subunit RPB10 19 c3letB_ 6.7 35 PDB header:transferase Chain: B: PDB Molecule:adenosine monophosphate-protein transferase vops; PDBTitle: crystal structure of fic domain containing ampylator, vops 20 c2ijoA_ 6.7 9 PDB header:hydrolase/hydrolase inhibitor Chain: A: PDB Molecule:polyprotein; PDBTitle: crystal structure of the west nile virus ns2b-ns3 protease2 complexed with bovine pancreatic trypsin inhibitor 21 d3ctda1 not modelled 6.3 7 Fold:post-AAA+ oligomerization domain-like Superfamily:post-AAA+ oligomerization domain-like Family:MgsA/YrvN C-terminal domain-like 22 c3e90C_ not modelled 6.2 9 PDB header:hydrolase Chain: C: PDB Molecule:ns2b cofactor; PDBTitle: west nile vi rus ns2b-ns3protease in complexed with2 inhibitor naph-kkr-h 23 d1osva_ not modelled 6.0 5 Fold:Nuclear receptor ligand-binding domain Superfamily:Nuclear receptor ligand-binding domain Family:Nuclear receptor ligand-binding domain 24 c1x7fA_ not modelled 5.8 21 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:outer surface protein; PDBTitle: crystal structure of an uncharacterized b. cereus protein 25 d1x6aa2 not modelled 5.6 14 Fold:Glucocorticoid receptor-like (DNA-binding domain) Superfamily:Glucocorticoid receptor-like (DNA-binding domain) Family:LIM domain 26 d2r9ga1 not modelled 5.5 14 Fold:post-AAA+ oligomerization domain-like Superfamily:post-AAA+ oligomerization domain-like Family:MgsA/YrvN C-terminal domain-like 27 c3et1A_ not modelled 5.4 15 PDB header:transcription/transferase Chain: A: PDB Molecule:peroxisome proliferator-activated receptor alpha; PDBTitle: structure of pparalpha with 3-[5-methoxy-1-(4-methoxy-2 benzenesulfonyl)-1h-indol-3-yl]-propionic acid

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0