Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	K	Q	K	E	L	W	I	N	Q	I	K	G	L	C	I	C	L	V	V	I	Y	H	S	V	I	T	F	Y	P	H	L	T	T	F	Q	H	P	L	S	E	V	L	S	K	C	W	I	Y	F	N	L	Y	L	A	P	F	R	M	P
Secondary structure
SS confidence
Disorder		?	?	?																												?							?			?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		V	F	F	F	I	S	G	Y	L	I	R	R	Y	I	D	S	V	P	W	G	N	C	L	D	K	R	I	W	N	I	F	W	V	L	A	L	W	G	V	V	Q	W	L	A	L	S	A	L	N	Q	W	L	A	P	E	R	D	L	S	N
Secondary structure
SS confidence
Disorder											?	?	?	?	?	?	?																																	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		A	S	N	A	A	Y	A	D	S	T	G	E	F	L	H	G	M	I	T	A	S	T	S	L	W	Y	L	Y	A	L	I	V	Y	F	V	V	C	K	I	F	S	R	L	A	L	P	L	F	A	L	F	V	L	L	S	V	A	V	N	F
Secondary structure
SS confidence
Disorder		?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		V	P	T	P	W	W	G	M	N	S	V	I	R	N	L	P	Y	Y	S	L	G	A	W	F	G	A	T	I	M	T	C	V	K	E	V	P	L	R	R	H	L	L	M	A	S	L	L	T	V	L	A	V	G	A	W	L	F	T	I	S
Secondary structure
SS confidence
Disorder		?	?	?	?	?																												?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		L	L	L	S	L	V	S	I	V	V	I	M	K	L	F	Y	Q	Y	E	Q	R	F	G	M	R	S	T	S	L	L	N	V	I	G	S	N	T	I	A	I	Y	T	T	H	R	I	L	V	E	I	F	S	L	T	L	L	A	Q	M	N
Secondary structure
SS confidence
Disorder																						?	?	?	?	?	?																																		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.
Sequence		A	A	R	W	S	P	Q	V	E	L	T	L	L	L	V	Y	P	F	V	S	L	F	I	C	T	V	A	G	L	L	V	R	K	L	S	Q	R	A	F	S	D	L	L	F	S	P	P	S	L	P	A	A	V	S	Y	S	R
Secondary structure
SS confidence
Disorder		?	?																																															?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2l3o chain A Region: 343 - 354 Aligned: 12 Modelled: 12 Confidence: 27.0% Identity: 50% PDB header:cytokine Chain: A: PDB Molecule:interleukin 3; PDBTitle: solution structure of murine interleukin 3 Phyre2 PDB 2wwb chain B Region: 270 - 287 Aligned: 18 Modelled: 18 Confidence: 15.5% Identity: 11% PDB header:ribosome Chain: B: PDB Molecule:protein transport protein sec61 subunit gamma; PDBTitle: cryo-em structure of the mammalian sec61 complex bound to the2 actively translating wheat germ 80s ribosome Phyre2 PDB 1rhz chain B Region: 270 - 291 Aligned: 22 Modelled: 22 Confidence: 13.0% Identity: 14% Fold: Single transmembrane helix Superfamily: Preprotein translocase SecE subunit Family: Preprotein translocase SecE subunit Phyre2 PDB 1h4u chain A Region: 339 - 356 Aligned: 18 Modelled: 18 Confidence: 12.1% Identity: 11% PDB header:extracellular matrix protein Chain: A: PDB Molecule:nidogen-1; PDBTitle: domain g2 of mouse nidogen-1 Phyre2 PDB 1gl4 chain A domain 1 Region: 339 - 356 Aligned: 18 Modelled: 18 Confidence: 10.2% Identity: 11% Fold: GFP-like Superfamily: GFP-like Family: Domain G2 of nidogen-1 Phyre2 PDB 3bhp chain A Region: 4 - 13 Aligned: 10 Modelled: 10 Confidence: 10.2% Identity: 0% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:upf0291 protein ynzc; PDBTitle: crystal structure of upf0291 protein ynzc from bacillus2 subtilis at resolution 2.0 a. northeast structural3 genomics consortium target sr384 Phyre2 PDB 3kdv chain B Region: 349 - 357 Aligned: 9 Modelled: 9 Confidence: 9.1% Identity: 89% PDB header:dna binding protein Chain: B: PDB Molecule:dna damage response b protein; PDBTitle: crystal structure of dna damage response b (ddrb) from deinococcus2 geothermalis Phyre2 PDB 1xrd chain A domain 1 Region: 315 - 354 Aligned: 40 Modelled: 40 Confidence: 8.9% Identity: 18% Fold: Light-harvesting complex subunits Superfamily: Light-harvesting complex subunits Family: Light-harvesting complex subunits Phyre2 PDB 1wu9 chain A domain 1 Region: 2 - 18 Aligned: 17 Modelled: 17 Confidence: 8.8% Identity: 6% Fold: EB1 dimerisation domain-like Superfamily: EB1 dimerisation domain-like Family: EB1 dimerisation domain-like Phyre2 PDB 1ujl chain A Region: 1 - 11 Aligned: 11 Modelled: 11 Confidence: 8.6% Identity: 18% PDB header:membrane protein Chain: A: PDB Molecule:potassium voltage-gated channel subfamily h PDBTitle: solution structure of the herg k+ channel s5-p2 extracellular linker Phyre2 PDB 1txq chain B domain 1 Region: 2 - 18 Aligned: 17 Modelled: 17 Confidence: 8.4% Identity: 6% Fold: EB1 dimerisation domain-like Superfamily: EB1 dimerisation domain-like Family: EB1 dimerisation domain-like Phyre2 PDB 1txq chain B Region: 2 - 18 Aligned: 17 Modelled: 17 Confidence: 8.4% Identity: 6% PDB header:structural protein/protein binding Chain: B: PDB Molecule:microtubule-associated protein rp/eb family PDBTitle: crystal structure of the eb1 c-terminal domain complexed2 with the cap-gly domain of p150glued Phyre2 PDB 2knc chain A Region: 301 - 334 Aligned: 34 Modelled: 34 Confidence: 8.4% Identity: 18% PDB header:cell adhesion Chain: A: PDB Molecule:integrin alpha-iib; PDBTitle: platelet integrin alfaiib-beta3 transmembrane-cytoplasmic2 heterocomplex Phyre2 PDB 2qna chain B Region: 3 - 15 Aligned: 13 Modelled: 13 Confidence: 8.0% Identity: 15% PDB header:transport protein Chain: B: PDB Molecule:snurportin-1; PDBTitle: crystal structure of human importin-beta (127-876) in complex with the2 ibb-domain of snurportin1 (1-65) Phyre2 PDB 2lat chain A Region: 9 - 30 Aligned: 22 Modelled: 22 Confidence: 7.6% Identity: 36% PDB header:membrane protein Chain: A: PDB Molecule:dolichyl-diphosphooligosaccharide--protein PDBTitle: solution structure of a human minimembrane protein ost4 Phyre2 PDB 2ys9 chain A Region: 331 - 354 Aligned: 24 Modelled: 24 Confidence: 7.6% Identity: 33% PDB header:transcription Chain: A: PDB Molecule:homeobox and leucine zipper protein homez; PDBTitle: structure of the third homeodomain from the human homeobox2 and leucine zipper protein, homez Phyre2 PDB 2eqo chain A Region: 333 - 343 Aligned: 11 Modelled: 11 Confidence: 7.4% Identity: 27% PDB header:transcription Chain: A: PDB Molecule:tnf receptor-associated factor 3-interacting PDBTitle: solution structure of the stn_traf3ip1_nd domain of2 interleukin 13 receptor alpha 1-binding protein-1 [homo3 sapiens] Phyre2 PDB 2gzd chain C Region: 1 - 11 Aligned: 11 Modelled: 11 Confidence: 7.3% Identity: 18% PDB header:protein transport Chain: C: PDB Molecule:rab11 family-interacting protein 2; PDBTitle: crystal structure of rab11 in complex with rab11-fip2 Phyre2 PDB 2cpg chain A Region: 1 - 5 Aligned: 5 Modelled: 5 Confidence: 7.3% Identity: 40% Fold: Ribbon-helix-helix Superfamily: Ribbon-helix-helix Family: CopG-like Phyre2 PDB 2k1a chain A Region: 301 - 334 Aligned: 34 Modelled: 34 Confidence: 6.9% Identity: 21% PDB header:cell adhesion Chain: A: PDB Molecule:integrin alpha-iib; PDBTitle: bicelle-embedded integrin alpha(iib) transmembrane segment Phyre2 1 c2l3oA_ 2 c2wwbB_ 3 d1rhzb_ 4 c1h4uA_ 5 d1gl4a1 6 c3bhpA_ 7 c3kdvB_ 8 d1xrda1 9 d1wu9a1 10 c1ujlA_ 11 d1txqb1 12 c1txqB_ 13 c2kncA_ 14 c2qnaB_ 15 c2latA_ 16 c2ys9A_ 17 c2eqoA_ 18 c2gzdC_ 19 d2cpga_ 20 c2k1aA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c2l3oA_ 27.0 50 PDB header:cytokine Chain: A: PDB Molecule:interleukin 3; PDBTitle: solution structure of murine interleukin 3 2 c2wwbB_ 15.5 11 PDB header:ribosome Chain: B: PDB Molecule:protein transport protein sec61 subunit gamma; PDBTitle: cryo-em structure of the mammalian sec61 complex bound to the2 actively translating wheat germ 80s ribosome 3 d1rhzb_ 13.0 14 Fold:Single transmembrane helix Superfamily:Preprotein translocase SecE subunit Family:Preprotein translocase SecE subunit 4 c1h4uA_ 12.1 11 PDB header:extracellular matrix protein Chain: A: PDB Molecule:nidogen-1; PDBTitle: domain g2 of mouse nidogen-1 5 d1gl4a1 10.2 11 Fold:GFP-like Superfamily:GFP-like Family:Domain G2 of nidogen-1 6 c3bhpA_ 10.2 0 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:upf0291 protein ynzc; PDBTitle: crystal structure of upf0291 protein ynzc from bacillus2 subtilis at resolution 2.0 a. northeast structural3 genomics consortium target sr384 7 c3kdvB_ 9.1 89 PDB header:dna binding protein Chain: B: PDB Molecule:dna damage response b protein; PDBTitle: crystal structure of dna damage response b (ddrb) from deinococcus2 geothermalis 8 d1xrda1 8.9 18 Fold:Light-harvesting complex subunits Superfamily:Light-harvesting complex subunits Family:Light-harvesting complex subunits 9 d1wu9a1 8.8 6 Fold:EB1 dimerisation domain-like Superfamily:EB1 dimerisation domain-like Family:EB1 dimerisation domain-like 10 c1ujlA_ 8.6 18 PDB header:membrane protein Chain: A: PDB Molecule:potassium voltage-gated channel subfamily h PDBTitle: solution structure of the herg k+ channel s5-p2 extracellular linker 11 d1txqb1 8.4 6 Fold:EB1 dimerisation domain-like Superfamily:EB1 dimerisation domain-like Family:EB1 dimerisation domain-like 12 c1txqB_ 8.4 6 PDB header:structural protein/protein binding Chain: B: PDB Molecule:microtubule-associated protein rp/eb family PDBTitle: crystal structure of the eb1 c-terminal domain complexed2 with the cap-gly domain of p150glued 13 c2kncA_ 8.4 18 PDB header:cell adhesion Chain: A: PDB Molecule:integrin alpha-iib; PDBTitle: platelet integrin alfaiib-beta3 transmembrane-cytoplasmic2 heterocomplex 14 c2qnaB_ 8.0 15 PDB header:transport protein Chain: B: PDB Molecule:snurportin-1; PDBTitle: crystal structure of human importin-beta (127-876) in complex with the2 ibb-domain of snurportin1 (1-65) 15 c2latA_ 7.6 36 PDB header:membrane protein Chain: A: PDB Molecule:dolichyl-diphosphooligosaccharide--protein PDBTitle: solution structure of a human minimembrane protein ost4 16 c2ys9A_ 7.6 33 PDB header:transcription Chain: A: PDB Molecule:homeobox and leucine zipper protein homez; PDBTitle: structure of the third homeodomain from the human homeobox2 and leucine zipper protein, homez 17 c2eqoA_ 7.4 27 PDB header:transcription Chain: A: PDB Molecule:tnf receptor-associated factor 3-interacting PDBTitle: solution structure of the stn_traf3ip1_nd domain of2 interleukin 13 receptor alpha 1-binding protein-1 [homo3 sapiens] 18 c2gzdC_ 7.3 18 PDB header:protein transport Chain: C: PDB Molecule:rab11 family-interacting protein 2; PDBTitle: crystal structure of rab11 in complex with rab11-fip2 19 d2cpga_ 7.3 40 Fold:Ribbon-helix-helix Superfamily:Ribbon-helix-helix Family:CopG-like 20 c2k1aA_ 6.9 21 PDB header:cell adhesion Chain: A: PDB Molecule:integrin alpha-iib; PDBTitle: bicelle-embedded integrin alpha(iib) transmembrane segment 21 c3a98B_ not modelled 6.9 17 PDB header:signaling protein Chain: B: PDB Molecule:engulfment and cell motility protein 1; PDBTitle: crystal structure of the complex of the interacting regions of dock22 and elmo1 22 c3sojA_ not modelled 6.8 20 PDB header:cell adhesion Chain: A: PDB Molecule:pile; PDBTitle: francisella tularensis pilin pile 23 c2qx7A_ not modelled 6.5 21 PDB header:plant protein Chain: A: PDB Molecule:eugenol synthase 1; PDBTitle: structure of eugenol synthase from ocimum basilicum 24 c2jvdA_ not modelled 6.4 0 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:upf0291 protein ynzc; PDBTitle: solution nmr structure of the folded n-terminal fragment of2 upf0291 protein ynzc from bacillus subtilis. northeast3 structural genomics target sr384-1-46 25 c2wssT_ not modelled 6.4 14 PDB header:hydrolase Chain: T: PDB Molecule:atp synthase subunit b, mitochondrial; PDBTitle: the structure of the membrane extrinsic region of bovine2 atp synthase 26 d1szia_ not modelled 6.2 19 Fold:Four-helical up-and-down bundle Superfamily:Mannose-6-phosphate receptor binding protein 1 (Tip47), C-terminal domain Family:Mannose-6-phosphate receptor binding protein 1 (Tip47), C-terminal domain 27 c2adlB_ not modelled 6.1 60 PDB header:dna binding protein Chain: B: PDB Molecule:ccda; PDBTitle: solution structure of the bacterial antitoxin ccda:2 implications for dna and toxin binding 28 c1jb0M_ not modelled 6.1 29 PDB header:photosynthesis Chain: M: PDB Molecule:photosystem 1 reaction centre subunit xii; PDBTitle: crystal structure of photosystem i: a photosynthetic reaction center2 and core antenna system from cyanobacteria 29 d1jb0m_ not modelled 6.1 29 Fold:Single transmembrane helix Superfamily:Subunit XII of photosystem I reaction centre, PsaM Family:Subunit XII of photosystem I reaction centre, PsaM 30 d1fc2c_ not modelled 6.0 30 Fold:immunoglobulin/albumin-binding domain-like Superfamily:Bacterial immunoglobulin/albumin-binding domains Family:Immunoglobulin-binding protein A modules 31 c2q5dD_ not modelled 5.9 10 PDB header:protein transport Chain: D: PDB Molecule:snurportin-1; PDBTitle: crystal structure of human importin beta bound to the2 snurportin1 ibb-domain second crystal form 32 d1iq8a4 not modelled 5.8 20 Fold:Cystatin-like Superfamily:Pre-PUA domain Family:Archaeosine tRNA-guanine transglycosylase, C2 domain 33 c2k6sB_ not modelled 5.6 20 PDB header:protein transport Chain: B: PDB Molecule:rab11fip2 protein; PDBTitle: structure of rab11-fip2 c-terminal coiled-coil domain 34 c3pvlB_ not modelled 5.6 45 PDB header:motor protein/protein transport Chain: B: PDB Molecule:usher syndrome type-1g protein; PDBTitle: structure of myosin viia myth4-ferm-sh3 in complex with the cen1 of2 sans 35 d1nkzb_ not modelled 5.6 13 Fold:Light-harvesting complex subunits Superfamily:Light-harvesting complex subunits Family:Light-harvesting complex subunits 36 c2hg5D_ not modelled 5.6 13 PDB header:membrane protein Chain: D: PDB Molecule:kcsa channel; PDBTitle: cs+ complex of a k channel with an amide to ester substitution in the2 selectivity filter 37 c3mtuD_ not modelled 5.5 6 PDB header:contractile protein Chain: D: PDB Molecule:tropomyosin alpha-1 chain, microtubule-associated protein PDBTitle: structure of the tropomyosin overlap complex from chicken smooth2 muscle 38 d1lp1b_ not modelled 5.5 30 Fold:immunoglobulin/albumin-binding domain-like Superfamily:Bacterial immunoglobulin/albumin-binding domains Family:Immunoglobulin-binding protein A modules 39 c2pbdV_ not modelled 5.5 71 PDB header:structural protein Chain: V: PDB Molecule:vasodilator-stimulated phosphoprotein; PDBTitle: ternary complex of profilin-actin with the poly-pro-gab2 domain of vasp* 40 c2ww9B_ not modelled 5.4 18 PDB header:ribosome Chain: B: PDB Molecule:protein transport protein sss1; PDBTitle: cryo-em structure of the active yeast ssh1 complex bound to the2 yeast 80s ribosome 41 d1prtf_ not modelled 5.3 21 Fold:OB-fold Superfamily:Bacterial enterotoxins Family:Bacterial AB5 toxins, B-subunits 42 d2f2fa1 not modelled 5.3 33 Fold:beta-Trefoil Superfamily:Ricin B-like lectins Family:Ricin B-like

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0