Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	K	S	T	E	F	H	P	V	H	Y	D	A	H	G	R	L	R	L	P	L	L	F	W	L	V	L	L	L	Q	A	R	T	W	V	L	F	V	I	A	G	A	S	R	E	Q	G	T	A	L	L	N	L	F	Y	P	D	H	D	N
Secondary structure
SS confidence
Disorder		?	?	?	?		?																																			?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		F	W	L	G	L	I	P	G	I	P	A	V	L	A	F	L	L	S	G	R	R	A	T	F	P	R	T	W	R	V	L	Y	F	L	L	L	L	A	Q	V	V	L	L	C	W	Q	P	W	L	W	L	N	G	E	S	V	S	G	I	G
Secondary structure
SS confidence
Disorder																								?	?	?																											?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.
Sequence		L	A	L	V	V	A	D	I	V	A	L	I	W	L	L	T	N	R	R	L	R	A	C	F	Y	E	V	K	E
Secondary structure
SS confidence
Disorder																										?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2bo9 chain B domain 2 Region: 12 - 37 Aligned: 26 Modelled: 26 Confidence: 27.6% Identity: 19% Fold: Cystatin-like Superfamily: Cystatin/monellin Family: Latexin-like Phyre2 PDB 1pcf chain A Region: 9 - 19 Aligned: 11 Modelled: 11 Confidence: 18.1% Identity: 18% Fold: ssDNA-binding transcriptional regulator domain Superfamily: ssDNA-binding transcriptional regulator domain Family: Transcriptional coactivator PC4 C-terminal domain Phyre2 PDB 1cks chain A Region: 2 - 14 Aligned: 13 Modelled: 13 Confidence: 14.0% Identity: 23% Fold: Cell cycle regulatory proteins Superfamily: Cell cycle regulatory proteins Family: Cell cycle regulatory proteins Phyre2 PDB 2bo9 chain B Region: 12 - 40 Aligned: 29 Modelled: 29 Confidence: 13.2% Identity: 17% PDB header:hydrolase Chain: B: PDB Molecule:human latexin; PDBTitle: human carboxypeptidase a4 in complex with human latexin. Phyre2 PDB 2lma chain A Region: 9 - 20 Aligned: 12 Modelled: 12 Confidence: 12.5% Identity: 25% PDB header:immune system Chain: A: PDB Molecule:thp5 peptide; PDBTitle: solution structure of cd4+ t cell derived peptide thp5 Phyre2 PDB 3bzk chain A domain 2 Region: 3 - 12 Aligned: 10 Modelled: 10 Confidence: 11.5% Identity: 30% Fold: SAM domain-like Superfamily: RuvA domain 2-like Family: Tex HhH-containing domain-like Phyre2 PDB 2k9p chain A Region: 61 - 110 Aligned: 49 Modelled: 50 Confidence: 11.1% Identity: 10% PDB header:membrane protein Chain: A: PDB Molecule:pheromone alpha factor receptor; PDBTitle: structure of tm1_tm2 in lppg micelles Phyre2 PDB 2ast chain C domain 1 Region: 3 - 14 Aligned: 12 Modelled: 12 Confidence: 9.5% Identity: 8% Fold: Cell cycle regulatory proteins Superfamily: Cell cycle regulatory proteins Family: Cell cycle regulatory proteins Phyre2 PDB 1puc chain A Region: 2 - 14 Aligned: 13 Modelled: 13 Confidence: 8.8% Identity: 15% Fold: Cell cycle regulatory proteins Superfamily: Cell cycle regulatory proteins Family: Cell cycle regulatory proteins Phyre2 PDB 1s6l chain A domain 1 Region: 11 - 17 Aligned: 7 Modelled: 7 Confidence: 7.8% Identity: 43% Fold: DNA/RNA-binding 3-helical bundle Superfamily: "Winged helix" DNA-binding domain Family: MerB N-terminal domain-like Phyre2 PDB 3qao chain A Region: 10 - 20 Aligned: 11 Modelled: 11 Confidence: 7.5% Identity: 45% PDB header:transcription regulator Chain: A: PDB Molecule:merr-like transcriptional regulator; PDBTitle: the crystal structure of the n-terminal domain of a merr-like2 transcriptional regulator from listeria monocytogenes egd-e Phyre2 PDB 1mg7 chain A domain 2 Region: 136 - 149 Aligned: 14 Modelled: 14 Confidence: 6.7% Identity: 21% Fold: Ferredoxin-like Superfamily: GHMP Kinase, C-terminal domain Family: Early switch protein XOL-1 Phyre2 PDB 1qb3 chain B Region: 2 - 14 Aligned: 13 Modelled: 13 Confidence: 6.5% Identity: 23% PDB header:cell cycle Chain: B: PDB Molecule:cyclin-dependent kinases regulatory subunit; PDBTitle: crystal structure of the cell cycle regulatory protein cks1 Phyre2 PDB 1y2t chain A Region: 2 - 12 Aligned: 11 Modelled: 11 Confidence: 6.1% Identity: 9% Fold: Cytolysin/lectin Superfamily: Cytolysin/lectin Family: Fungal fruit body lectin Phyre2 PDB 2oce chain A Region: 3 - 12 Aligned: 10 Modelled: 10 Confidence: 5.9% Identity: 30% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical protein pa5201; PDBTitle: crystal structure of tex family protein pa5201 from2 pseudomonas aeruginosa Phyre2 PDB 1x99 chain A Region: 7 - 16 Aligned: 10 Modelled: 10 Confidence: 5.7% Identity: 40% Fold: Cytolysin/lectin Superfamily: Cytolysin/lectin Family: Fungal fruit body lectin Phyre2 PDB 1qb3 chain A Region: 2 - 14 Aligned: 13 Modelled: 13 Confidence: 5.7% Identity: 23% Fold: Cell cycle regulatory proteins Superfamily: Cell cycle regulatory proteins Family: Cell cycle regulatory proteins Phyre2 PDB 3d6z chain A Region: 10 - 19 Aligned: 10 Modelled: 10 Confidence: 5.4% Identity: 20% PDB header:transcription regulator/dna Chain: A: PDB Molecule:multidrug-efflux transporter 1 regulator; PDBTitle: crystal structure of r275e mutant of bmrr bound to dna and rhodamine Phyre2 1 d2bo9b2 2 d1pcfa_ 3 d1cksa_ 4 c2bo9B_ 5 c2lmaA_ 6 d3bzka2 7 c2k9pA_ 8 d2astc1 9 d1puca_ 10 d1s6la1 11 c3qaoA_ 12 d1mg7a2 13 c1qb3B_ 14 d1y2ta_ 15 c2oceA_ 16 d1x99a_ 17 d1qb3a_ 18 c3d6zA_

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d2bo9b2 27.6 19 Fold:Cystatin-like Superfamily:Cystatin/monellin Family:Latexin-like 2 d1pcfa_ 18.1 18 Fold:ssDNA-binding transcriptional regulator domain Superfamily:ssDNA-binding transcriptional regulator domain Family:Transcriptional coactivator PC4 C-terminal domain 3 d1cksa_ 14.0 23 Fold:Cell cycle regulatory proteins Superfamily:Cell cycle regulatory proteins Family:Cell cycle regulatory proteins 4 c2bo9B_ 13.2 17 PDB header:hydrolase Chain: B: PDB Molecule:human latexin; PDBTitle: human carboxypeptidase a4 in complex with human latexin. 5 c2lmaA_ 12.5 25 PDB header:immune system Chain: A: PDB Molecule:thp5 peptide; PDBTitle: solution structure of cd4+ t cell derived peptide thp5 6 d3bzka2 11.5 30 Fold:SAM domain-like Superfamily:RuvA domain 2-like Family:Tex HhH-containing domain-like 7 c2k9pA_ 11.1 10 PDB header:membrane protein Chain: A: PDB Molecule:pheromone alpha factor receptor; PDBTitle: structure of tm1_tm2 in lppg micelles 8 d2astc1 9.5 8 Fold:Cell cycle regulatory proteins Superfamily:Cell cycle regulatory proteins Family:Cell cycle regulatory proteins 9 d1puca_ 8.8 15 Fold:Cell cycle regulatory proteins Superfamily:Cell cycle regulatory proteins Family:Cell cycle regulatory proteins 10 d1s6la1 7.8 43 Fold:DNA/RNA-binding 3-helical bundle Superfamily:"Winged helix" DNA-binding domain Family:MerB N-terminal domain-like 11 c3qaoA_ 7.5 45 PDB header:transcription regulator Chain: A: PDB Molecule:merr-like transcriptional regulator; PDBTitle: the crystal structure of the n-terminal domain of a merr-like2 transcriptional regulator from listeria monocytogenes egd-e 12 d1mg7a2 6.7 21 Fold:Ferredoxin-like Superfamily:GHMP Kinase, C-terminal domain Family:Early switch protein XOL-1 13 c1qb3B_ 6.5 23 PDB header:cell cycle Chain: B: PDB Molecule:cyclin-dependent kinases regulatory subunit; PDBTitle: crystal structure of the cell cycle regulatory protein cks1 14 d1y2ta_ 6.1 9 Fold:Cytolysin/lectin Superfamily:Cytolysin/lectin Family:Fungal fruit body lectin 15 c2oceA_ 5.9 30 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical protein pa5201; PDBTitle: crystal structure of tex family protein pa5201 from2 pseudomonas aeruginosa 16 d1x99a_ 5.7 40 Fold:Cytolysin/lectin Superfamily:Cytolysin/lectin Family:Fungal fruit body lectin 17 d1qb3a_ 5.7 23 Fold:Cell cycle regulatory proteins Superfamily:Cell cycle regulatory proteins Family:Cell cycle regulatory proteins 18 c3d6zA_ 5.4 20 PDB header:transcription regulator/dna Chain: A: PDB Molecule:multidrug-efflux transporter 1 regulator; PDBTitle: crystal structure of r275e mutant of bmrr bound to dna and rhodamine

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0