Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	L	Q	F	I	L	R	R	L	G	L	V	I	P	T	F	I	G	I	T	L	L	T	F	A	F	V	H	M	I	P	G	D	P	V	M	I	M	A	G	E	R	G	I	S	P	E	R	H	A	Q	L	L	A	E	L	G	L	D	K
Secondary structure
SS confidence
Disorder		?																																							?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		P	M	W	Q	Q	Y	L	H	Y	I	W	G	V	M	H	G	D	L	G	I	S	M	K	S	R	I	P	V	W	E	E	F	V	P	R	F	Q	A	T	L	E	L	G	V	C	A	M	I	F	A	T	A	V	G	I	P	V	G	V	L
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		A	A	V	K	R	G	S	I	F	D	H	T	A	V	G	L	A	L	T	G	Y	S	M	P	I	F	W	W	G	M	M	L	I	M	L	V	S	V	H	W	N	L	T	P	V	S	G	R	V	S	D	M	V	F	L	D	D	S	N	P
Secondary structure
SS confidence
Disorder																																																		?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		L	T	G	F	M	L	I	D	T	A	I	W	G	E	D	G	N	F	I	D	A	V	A	H	M	I	L	P	A	I	V	L	G	T	I	P	L	A	V	I	V	R	M	T	R	S	S	M	L	E	V	L	G	E	D	Y	I	R	T	A
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		R	A	K	G	L	T	R	M	R	V	I	I	V	H	A	L	R	N	A	M	L	P	V	V	T	V	I	G	L	Q	V	G	T	L	L	A	G	A	I	L	T	E	T	I	F	S	W	P	G	L	G	R	W	L	I	D	A	L	Q	R
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.
Sequence		R	D	Y	P	V	V	Q	G	G	V	L	L	V	A	T	M	I	I	L	V	N	L	L	V	D	L	L	Y	G	V	V	N	P	R	I	R	H	K	K
Secondary structure
SS confidence
Disorder																																		?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 3dhw chain A domain 1 Region: 91 - 325 Aligned: 194 Modelled: 196 Confidence: 99.9% Identity: 23% Fold: MetI-like Superfamily: MetI-like Family: MetI-like Phyre2 PDB 2onk chain C domain 1 Region: 90 - 327 Aligned: 205 Modelled: 207 Confidence: 99.8% Identity: 12% Fold: MetI-like Superfamily: MetI-like Family: MetI-like Phyre2 PDB 2onk chain C Region: 90 - 327 Aligned: 205 Modelled: 207 Confidence: 99.8% Identity: 12% PDB header:membrane protein Chain: C: PDB Molecule:molybdate/tungstate abc transporter, permease PDBTitle: abc transporter modbc in complex with its binding protein2 moda Phyre2 PDB 3d31 chain D Region: 90 - 324 Aligned: 204 Modelled: 206 Confidence: 99.7% Identity: 11% PDB header:transport protein Chain: D: PDB Molecule:sulfate/molybdate abc transporter, permease PDBTitle: modbc from methanosarcina acetivorans Phyre2 PDB 3d31 chain C domain 1 Region: 90 - 324 Aligned: 204 Modelled: 206 Confidence: 99.7% Identity: 11% Fold: MetI-like Superfamily: MetI-like Family: MetI-like Phyre2 PDB 2r6g chain F domain 2 Region: 84 - 323 Aligned: 218 Modelled: 220 Confidence: 99.6% Identity: 12% Fold: MetI-like Superfamily: MetI-like Family: MetI-like Phyre2 PDB 3fh6 chain F Region: 1 - 339 Aligned: 289 Modelled: 319 Confidence: 99.6% Identity: 15% PDB header:transport protein Chain: F: PDB Molecule:maltose transport system permease protein malf; PDBTitle: crystal structure of the resting state maltose transporter from e.2 coli Phyre2 PDB 2r6g chain F Region: 84 - 323 Aligned: 218 Modelled: 220 Confidence: 99.5% Identity: 12% PDB header:hydrolase/transport protein Chain: F: PDB Molecule:maltose transport system permease protein malf; PDBTitle: the crystal structure of the e. coli maltose transporter Phyre2 PDB 2r6g chain G domain 1 Region: 6 - 324 Aligned: 260 Modelled: 276 Confidence: 99.1% Identity: 13% Fold: MetI-like Superfamily: MetI-like Family: MetI-like Phyre2 PDB 1cii chain A Region: 75 - 132 Aligned: 55 Modelled: 58 Confidence: 26.3% Identity: 16% PDB header:transmembrane protein Chain: A: PDB Molecule:colicin ia; PDBTitle: colicin ia Phyre2 PDB 3edn chain B Region: 32 - 60 Aligned: 29 Modelled: 29 Confidence: 26.2% Identity: 21% PDB header:biosynthetic protein Chain: B: PDB Molecule:phenazine biosynthesis protein, phzf family; PDBTitle: crystal structure of the bacillus anthracis phenazine2 biosynthesis protein, phzf family Phyre2 PDB 1qy9 chain A domain 1 Region: 32 - 60 Aligned: 29 Modelled: 29 Confidence: 26.2% Identity: 17% Fold: Diaminopimelate epimerase-like Superfamily: Diaminopimelate epimerase-like Family: PhzC/PhzF-like Phyre2 PDB 2ka1 chain A Region: 100 - 124 Aligned: 25 Modelled: 25 Confidence: 24.4% Identity: 12% PDB header:membrane protein Chain: A: PDB Molecule:bcl2/adenovirus e1b 19 kda protein-interacting PDBTitle: solution nmr structure of bnip3 transmembrane peptide dimer2 in detergent micelles Phyre2 PDB 2ka2 chain B Region: 100 - 124 Aligned: 25 Modelled: 25 Confidence: 24.4% Identity: 12% PDB header:membrane protein Chain: B: PDB Molecule:bcl2/adenovirus e1b 19 kda protein-interacting PDBTitle: solution nmr structure of bnip3 transmembrane peptide dimer2 in detergent micelles with his173-ser172 intermonomer3 hydrogen bond restraints Phyre2 PDB 2ka2 chain A Region: 100 - 124 Aligned: 25 Modelled: 25 Confidence: 24.4% Identity: 12% PDB header:membrane protein Chain: A: PDB Molecule:bcl2/adenovirus e1b 19 kda protein-interacting PDBTitle: solution nmr structure of bnip3 transmembrane peptide dimer2 in detergent micelles with his173-ser172 intermonomer3 hydrogen bond restraints Phyre2 PDB 2ka1 chain B Region: 100 - 124 Aligned: 25 Modelled: 25 Confidence: 24.4% Identity: 12% PDB header:membrane protein Chain: B: PDB Molecule:bcl2/adenovirus e1b 19 kda protein-interacting PDBTitle: solution nmr structure of bnip3 transmembrane peptide dimer2 in detergent micelles Phyre2 PDB 1xub chain A domain 1 Region: 32 - 60 Aligned: 29 Modelled: 29 Confidence: 23.5% Identity: 21% Fold: Diaminopimelate epimerase-like Superfamily: Diaminopimelate epimerase-like Family: PhzC/PhzF-like Phyre2 PDB 1u0k chain A Region: 32 - 60 Aligned: 29 Modelled: 29 Confidence: 21.3% Identity: 10% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:gene product pa4716; PDBTitle: the structure of a predicted epimerase pa4716 from pseudomonas2 aeruginosa Phyre2 PDB 1u0k chain A domain 1 Region: 32 - 60 Aligned: 29 Modelled: 29 Confidence: 20.1% Identity: 10% Fold: Diaminopimelate epimerase-like Superfamily: Diaminopimelate epimerase-like Family: PhzC/PhzF-like Phyre2 PDB 1s7j chain A Region: 32 - 60 Aligned: 29 Modelled: 29 Confidence: 19.6% Identity: 17% Fold: Diaminopimelate epimerase-like Superfamily: Diaminopimelate epimerase-like Family: PhzC/PhzF-like Phyre2 1 d3dhwa1 2 d2onkc1 3 c2onkC_ 4 c3d31D_ 5 d3d31c1 6 d2r6gf2 7 c3fh6F_ 8 c2r6gF_ 9 d2r6gg1 10 c1ciiA_ 11 c3ednB_ 12 d1qy9a1 13 c2ka1A_ 14 c2ka2B_ 15 c2ka2A_ 16 c2ka1B_ 17 d1xuba1 18 c1u0kA_ 19 d1u0ka1 20 d1s7ja_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d3dhwa1 99.9 23 Fold:MetI-like Superfamily:MetI-like Family:MetI-like 2 d2onkc1 99.8 12 Fold:MetI-like Superfamily:MetI-like Family:MetI-like 3 c2onkC_ 99.8 12 PDB header:membrane protein Chain: C: PDB Molecule:molybdate/tungstate abc transporter, permease PDBTitle: abc transporter modbc in complex with its binding protein2 moda 4 c3d31D_ 99.7 11 PDB header:transport protein Chain: D: PDB Molecule:sulfate/molybdate abc transporter, permease PDBTitle: modbc from methanosarcina acetivorans 5 d3d31c1 99.7 11 Fold:MetI-like Superfamily:MetI-like Family:MetI-like 6 d2r6gf2 99.6 12 Fold:MetI-like Superfamily:MetI-like Family:MetI-like 7 c3fh6F_ 99.6 15 PDB header:transport protein Chain: F: PDB Molecule:maltose transport system permease protein malf; PDBTitle: crystal structure of the resting state maltose transporter from e.2 coli 8 c2r6gF_ 99.5 12 PDB header:hydrolase/transport protein Chain: F: PDB Molecule:maltose transport system permease protein malf; PDBTitle: the crystal structure of the e. coli maltose transporter 9 d2r6gg1 99.1 13 Fold:MetI-like Superfamily:MetI-like Family:MetI-like 10 c1ciiA_ 26.3 16 PDB header:transmembrane protein Chain: A: PDB Molecule:colicin ia; PDBTitle: colicin ia 11 c3ednB_ 26.2 21 PDB header:biosynthetic protein Chain: B: PDB Molecule:phenazine biosynthesis protein, phzf family; PDBTitle: crystal structure of the bacillus anthracis phenazine2 biosynthesis protein, phzf family 12 d1qy9a1 26.2 17 Fold:Diaminopimelate epimerase-like Superfamily:Diaminopimelate epimerase-like Family:PhzC/PhzF-like 13 c2ka1A_ 24.4 12 PDB header:membrane protein Chain: A: PDB Molecule:bcl2/adenovirus e1b 19 kda protein-interacting PDBTitle: solution nmr structure of bnip3 transmembrane peptide dimer2 in detergent micelles 14 c2ka2B_ 24.4 12 PDB header:membrane protein Chain: B: PDB Molecule:bcl2/adenovirus e1b 19 kda protein-interacting PDBTitle: solution nmr structure of bnip3 transmembrane peptide dimer2 in detergent micelles with his173-ser172 intermonomer3 hydrogen bond restraints 15 c2ka2A_ 24.4 12 PDB header:membrane protein Chain: A: PDB Molecule:bcl2/adenovirus e1b 19 kda protein-interacting PDBTitle: solution nmr structure of bnip3 transmembrane peptide dimer2 in detergent micelles with his173-ser172 intermonomer3 hydrogen bond restraints 16 c2ka1B_ 24.4 12 PDB header:membrane protein Chain: B: PDB Molecule:bcl2/adenovirus e1b 19 kda protein-interacting PDBTitle: solution nmr structure of bnip3 transmembrane peptide dimer2 in detergent micelles 17 d1xuba1 23.5 21 Fold:Diaminopimelate epimerase-like Superfamily:Diaminopimelate epimerase-like Family:PhzC/PhzF-like 18 c1u0kA_ 21.3 10 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:gene product pa4716; PDBTitle: the structure of a predicted epimerase pa4716 from pseudomonas2 aeruginosa 19 d1u0ka1 20.1 10 Fold:Diaminopimelate epimerase-like Superfamily:Diaminopimelate epimerase-like Family:PhzC/PhzF-like 20 d1s7ja_ 19.6 17 Fold:Diaminopimelate epimerase-like Superfamily:Diaminopimelate epimerase-like Family:PhzC/PhzF-like 21 c2j5dA_ not modelled 19.5 12 PDB header:membrane protein Chain: A: PDB Molecule:bcl2/adenovirus e1b 19 kda protein-interacting PDBTitle: nmr structure of bnip3 transmembrane domain in lipid2 bicelles 22 c1u1wA_ not modelled 19.4 21 PDB header:isomerase, lyase Chain: A: PDB Molecule:phenazine biosynthesis protein phzf; PDBTitle: structure and function of phenazine-biosynthesis protein phzf from2 pseudomonas fluorescens 2-79 23 c1wz4A_ not modelled 19.4 38 PDB header:gene regulation Chain: A: PDB Molecule:major surface antigen; PDBTitle: solution conformation of adr subtype hbv pre-s2 epitope 24 c1qy9B_ not modelled 17.1 17 PDB header:unknown function Chain: B: PDB Molecule:hypothetical protein ydde; PDBTitle: crystal structure of e. coli se-met protein ydde 25 d2d6fc1 not modelled 16.9 15 Fold:GatB/YqeY motif Superfamily:GatB/YqeY motif Family:GatB/GatE C-terminal domain-like 26 c2cw1A_ not modelled 16.1 32 PDB header:de novo protein Chain: A: PDB Molecule:sn4m; PDBTitle: solution structure of the de novo-designed lambda cro fold2 protein 27 c2iv1J_ not modelled 16.0 22 PDB header:lyase Chain: J: PDB Molecule:cyanate hydratase; PDBTitle: site directed mutagenesis of key residues involved in the2 catalytic mechanism of cyanase 28 c1y66D_ not modelled 13.6 21 PDB header:de novo protein Chain: D: PDB Molecule:engrailed homeodomain; PDBTitle: dioxane contributes to the altered conformation and2 oligomerization state of a designed engrailed homeodomain3 variant 29 c2hw2A_ not modelled 13.0 37 PDB header:transferase Chain: A: PDB Molecule:rifampin adp-ribosyl transferase; PDBTitle: crystal structure of rifampin adp-ribosyl transferase in2 complex with rifampin 30 d1a9xa1 not modelled 12.9 19 Fold:Carbamoyl phosphate synthetase, large subunit connection domain Superfamily:Carbamoyl phosphate synthetase, large subunit connection domain Family:Carbamoyl phosphate synthetase, large subunit connection domain 31 c1ym5A_ not modelled 10.6 24 PDB header:oxidoreductase Chain: A: PDB Molecule:hypothetical 32.6 kda protein in dap2-slt2 PDBTitle: crystal structure of yhi9, the yeast member of the2 phenazine biosynthesis phzf enzyme superfamily. 32 c2v9kA_ not modelled 9.6 32 PDB header:lyase Chain: A: PDB Molecule:uncharacterized protein flj32312; PDBTitle: crystal structure of human pus10, a novel pseudouridine2 synthase. 33 d1n0ua5 not modelled 8.8 23 Fold:Ferredoxin-like Superfamily:EF-G C-terminal domain-like Family:EF-G/eEF-2 domains III and V 34 d2oara1 not modelled 8.3 13 Fold:Gated mechanosensitive channel Superfamily:Gated mechanosensitive channel Family:Gated mechanosensitive channel 35 d1dwka1 not modelled 8.0 19 Fold:lambda repressor-like DNA-binding domains Superfamily:lambda repressor-like DNA-binding domains Family:Cyanase N-terminal domain 36 d1g2ha_ not modelled 7.6 13 Fold:DNA/RNA-binding 3-helical bundle Superfamily:Homeodomain-like Family:FIS-like 37 c2b9sB_ not modelled 6.7 38 PDB header:isomerase/dna Chain: B: PDB Molecule:dna topoisomerase i-like protein; PDBTitle: crystal structure of heterodimeric l. donovani2 topoisomerase i-vanadate-dna complex 38 c2oarA_ not modelled 6.6 13 PDB header:membrane protein Chain: A: PDB Molecule:large-conductance mechanosensitive channel; PDBTitle: mechanosensitive channel of large conductance (mscl) 39 c1umqA_ not modelled 6.4 19 PDB header:dna-binding protein Chain: A: PDB Molecule:photosynthetic apparatus regulatory protein; PDBTitle: solution structure and dna binding of the effector domain2 from the global regulator prra(rega) from r. sphaeroides:3 insights into dna binding specificity 40 d1umqa_ not modelled 6.4 19 Fold:DNA/RNA-binding 3-helical bundle Superfamily:Homeodomain-like Family:FIS-like 41 d1ddfa_ not modelled 5.8 6 Fold:DEATH domain Superfamily:DEATH domain Family:DEATH domain, DD 42 d2auwa1 not modelled 5.7 29 Fold:lambda repressor-like DNA-binding domains Superfamily:lambda repressor-like DNA-binding domains Family:NE0471 C-terminal domain-like 43 d1gt1a_ not modelled 5.5 18 Fold:Lipocalins Superfamily:Lipocalins Family:Retinol binding protein-like 44 c2knaA_ not modelled 5.5 10 PDB header:apoptosis Chain: A: PDB Molecule:baculoviral iap repeat-containing protein 4; PDBTitle: solution structure of uba domain of xiap

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0