Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	F	P	V	S	S	I	G	N	D	I	S	S	D	L	V	R	R	K	M	N	D	L	P	E	S	P	T	G	N	N	L	E	A	L	A	P	G	I	E	K	L	K	Q	T	S	I	E	M	V	T	L	L	N	T	L	Q	P	G	G
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?																?	?	?		?			?	?	?				?	?																?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		K	C	I	I	T	G	D	F	Q	K	E	L	A	Y	L	Q	N	V	I	L	Y	N	V	S	S	L	R	L	D	F	L	G	Y	N	A	Q	I	I	Q	R	S	D	N	T	C	E	L	T	I	N	E	P	L	K	N	Q	E	I	S	T
Secondary structure
SS confidence
Disorder																																																							?		?	?		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		G	N	I	N	I	N	C	P	L	K	D	I	Y	N	E	I	R	R	L	N	V	I	F	S	C	G	T	G	D	I	V	D	L	S	S	L	D	L	R	N	V	D	L	D	Y	Y	D	F	T	D	K	H	M	A	N	T	I	L	N	P
Secondary structure
SS confidence
Disorder		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		F	K	L	N	S	T	N	F	T	N	A	N	M	F	Q	V	N	F	V	S	S	T	Q	N	A	T	I	S	W	D	Y	L	L	K	I	T	P	V	L	I	S	I	S	D	M	Y	S	E	E	K	I	K	F	V	E	S	C	L	N	E
Secondary structure
SS confidence
Disorder				?																																										?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		P	G	D	I	T	E	E	Q	L	K	I	M	R	F	A	I	I	K	S	I	P	R	A	T	L	T	D	K	L	E	N	E	L	T	K	E	I	Y	K	S	S	S	K	I	I	N	C	L	N	R	I	K	L	T	E	M	K	E	F	S
Secondary structure
SS confidence
Disorder				?	?											?							?																																?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		S	E	K	I	Y	D	Y	I	D	I	I	I	E	D	Y	E	N	T	K	E	N	A	Y	L	V	V	P	Q	I	N	Y	T	M	D	L	N	I	E	D	S	S	S	E	E	L	L	S	D	N	T	L	E	K	D	E	N	S	P	D	N
Secondary structure
SS confidence
Disorder																																										?	?			?		?	?	?	?		?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.
Sequence		G	F	E	V	G	E	Y	N	T	Y	E	A	Y	N	S	E	K	Q	Y	F	T	R	E	D	Y	T	Y	D	Y	D	L	L	N	A	I
Secondary structure
SS confidence
Disorder		?											?			?						?												?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 3du1 chain X Region: 151 - 193 Aligned: 43 Modelled: 43 Confidence: 34.3% Identity: 19% PDB header:structural protein Chain: X: PDB Molecule:all3740 protein; PDBTitle: the 2.0 angstrom resolution crystal structure of hetl, a pentapeptide2 repeat protein involved in heterocyst differentiation regulation from3 the cyanobacterium nostoc sp. strain pcc 7120 Phyre2 PDB 3nb2 chain B Region: 152 - 198 Aligned: 47 Modelled: 47 Confidence: 33.9% Identity: 23% PDB header:ligase Chain: B: PDB Molecule:secreted effector protein; PDBTitle: crystal structure of e. coli o157:h7 effector protein nlel Phyre2 PDB 2xtw chain B Region: 151 - 209 Aligned: 58 Modelled: 59 Confidence: 18.7% Identity: 12% PDB header:cell cycle Chain: B: PDB Molecule:qnrb1; PDBTitle: structure of qnrb1 (full length), a plasmid-mediated2 fluoroquinolone resistance protein Phyre2 PDB 2j8i chain A domain 1 Region: 151 - 193 Aligned: 43 Modelled: 43 Confidence: 17.6% Identity: 23% Fold: Single-stranded right-handed beta-helix Superfamily: Pentapeptide repeat-like Family: Pentapeptide repeats Phyre2 PDB 2xt4 chain B Region: 151 - 201 Aligned: 51 Modelled: 51 Confidence: 11.9% Identity: 18% PDB header:cell cycle Chain: B: PDB Molecule:mcbg-like protein; PDBTitle: structure of the pentapeptide repeat protein albg, a2 resistance factor for the topoisomerase poison albicidin. Phyre2 PDB 1z0a chain A domain 1 Region: 208 - 218 Aligned: 11 Modelled: 11 Confidence: 11.6% Identity: 27% Fold: P-loop containing nucleoside triphosphate hydrolases Superfamily: P-loop containing nucleoside triphosphate hydrolases Family: G proteins Phyre2 PDB 2j8i chain B Region: 151 - 204 Aligned: 54 Modelled: 54 Confidence: 10.9% Identity: 17% PDB header:toxin Chain: B: PDB Molecule:np275; PDBTitle: structure of np275, a pentapeptide repeat protein from2 nostoc punctiforme Phyre2 PDB 2nac chain A domain 2 Region: 116 - 161 Aligned: 46 Modelled: 46 Confidence: 10.7% Identity: 33% Fold: Flavodoxin-like Superfamily: Formate/glycerate dehydrogenase catalytic domain-like Family: Formate/glycerate dehydrogenases, substrate-binding domain Phyre2 PDB 3r67 chain A Region: 140 - 182 Aligned: 35 Modelled: 43 Confidence: 10.6% Identity: 29% PDB header:hydrolase Chain: A: PDB Molecule:putative glycosidase; PDBTitle: crystal structure of a putative glycosidase (bt_4094) from bacteroides2 thetaiotaomicron vpi-5482 at 2.30 a resolution Phyre2 PDB 2j8k chain A domain 1 Region: 152 - 209 Aligned: 58 Modelled: 58 Confidence: 10.4% Identity: 17% Fold: Single-stranded right-handed beta-helix Superfamily: Pentapeptide repeat-like Family: Pentapeptide repeats Phyre2 PDB 1wmx chain A Region: 92 - 153 Aligned: 62 Modelled: 62 Confidence: 10.3% Identity: 19% Fold: Galactose-binding domain-like Superfamily: Galactose-binding domain-like Family: Family 30 carbohydrate binding module, CBM30 (PKD repeat) Phyre2 PDB 2b4w chain A domain 1 Region: 140 - 186 Aligned: 36 Modelled: 47 Confidence: 10.1% Identity: 50% Fold: 5-bladed beta-propeller Superfamily: Arabinanase/levansucrase/invertase Family: LmjF10.1260-like Phyre2 PDB 2o6w chain A Region: 151 - 206 Aligned: 56 Modelled: 56 Confidence: 9.6% Identity: 16% PDB header:unknown function Chain: A: PDB Molecule:repeat five residue (rfr) protein or PDBTitle: crystal structure of a pentapeptide repeat protein (rfr23)2 from the cyanobacterium cyanothece 51142 Phyre2 PDB 3pss chain B Region: 151 - 201 Aligned: 51 Modelled: 51 Confidence: 9.0% Identity: 14% PDB header:cell cycle Chain: B: PDB Molecule:qnr; PDBTitle: crystal structure of ahqnr, the qnr protein from aeromonas hydrophila2 (p21 crystal form) Phyre2 PDB 2yui chain A Region: 52 - 80 Aligned: 29 Modelled: 29 Confidence: 8.5% Identity: 21% PDB header:apoptosis Chain: A: PDB Molecule:anamorsin; PDBTitle: solution structure of the n-terminal domain in human2 cytokine-induced apoptosis inhibitor anamorsin Phyre2 PDB 3n90 chain A Region: 151 - 198 Aligned: 48 Modelled: 48 Confidence: 8.2% Identity: 23% PDB header:unknown function Chain: A: PDB Molecule:thylakoid lumenal 15 kda protein 1, chloroplastic; PDBTitle: the 1.7 angstrom resolution crystal structure of at2g44920, a2 pentapeptide repeat protein from arabidopsis thaliana thylakoid3 lumen. Phyre2 PDB 2f3l chain A domain 1 Region: 151 - 201 Aligned: 51 Modelled: 51 Confidence: 8.0% Identity: 18% Fold: Single-stranded right-handed beta-helix Superfamily: Pentapeptide repeat-like Family: Pentapeptide repeats Phyre2 PDB 2w7z chain B Region: 138 - 202 Aligned: 65 Modelled: 65 Confidence: 7.8% Identity: 15% PDB header:inhibitor Chain: B: PDB Molecule:pentapeptide repeat family protein; PDBTitle: structure of the pentapeptide repeat protein efsqnr, a dna2 gyrase inhibitor. free amines modified by cyclic3 pentylation with glutaraldehyde. Phyre2 PDB 2zjr chain J domain 1 Region: 240 - 262 Aligned: 23 Modelled: 23 Confidence: 7.7% Identity: 26% Fold: alpha/beta-Hammerhead Superfamily: Ribosomal protein L16p/L10e Family: Ribosomal protein L16p Phyre2 PDB 2bm5 chain A domain 1 Region: 152 - 202 Aligned: 51 Modelled: 51 Confidence: 7.7% Identity: 12% Fold: Single-stranded right-handed beta-helix Superfamily: Pentapeptide repeat-like Family: Pentapeptide repeats Phyre2 1 c3du1X_ 2 c3nb2B_ 3 c2xtwB_ 4 d2j8ia1 5 c2xt4B_ 6 d1z0aa1 7 c2j8iB_ 8 d2naca2 9 c3r67A_ 10 d2j8ka1 11 d1wmxa_ 12 d2b4wa1 13 c2o6wA_ 14 c3pssB_ 15 c2yuiA_ 16 c3n90A_ 17 d2f3la1 18 c2w7zB_ 19 d2zjrj1 20 d2bm5a1 21 22 23 24 25 26 27 28 29 30 31 32

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c3du1X_ 34.3 19 PDB header:structural protein Chain: X: PDB Molecule:all3740 protein; PDBTitle: the 2.0 angstrom resolution crystal structure of hetl, a pentapeptide2 repeat protein involved in heterocyst differentiation regulation from3 the cyanobacterium nostoc sp. strain pcc 7120 2 c3nb2B_ 33.9 23 PDB header:ligase Chain: B: PDB Molecule:secreted effector protein; PDBTitle: crystal structure of e. coli o157:h7 effector protein nlel 3 c2xtwB_ 18.7 12 PDB header:cell cycle Chain: B: PDB Molecule:qnrb1; PDBTitle: structure of qnrb1 (full length), a plasmid-mediated2 fluoroquinolone resistance protein 4 d2j8ia1 17.6 23 Fold:Single-stranded right-handed beta-helix Superfamily:Pentapeptide repeat-like Family:Pentapeptide repeats 5 c2xt4B_ 11.9 18 PDB header:cell cycle Chain: B: PDB Molecule:mcbg-like protein; PDBTitle: structure of the pentapeptide repeat protein albg, a2 resistance factor for the topoisomerase poison albicidin. 6 d1z0aa1 11.6 27 Fold:P-loop containing nucleoside triphosphate hydrolases Superfamily:P-loop containing nucleoside triphosphate hydrolases Family:G proteins 7 c2j8iB_ 10.9 17 PDB header:toxin Chain: B: PDB Molecule:np275; PDBTitle: structure of np275, a pentapeptide repeat protein from2 nostoc punctiforme 8 d2naca2 10.7 33 Fold:Flavodoxin-like Superfamily:Formate/glycerate dehydrogenase catalytic domain-like Family:Formate/glycerate dehydrogenases, substrate-binding domain 9 c3r67A_ 10.6 29 PDB header:hydrolase Chain: A: PDB Molecule:putative glycosidase; PDBTitle: crystal structure of a putative glycosidase (bt_4094) from bacteroides2 thetaiotaomicron vpi-5482 at 2.30 a resolution 10 d2j8ka1 10.4 17 Fold:Single-stranded right-handed beta-helix Superfamily:Pentapeptide repeat-like Family:Pentapeptide repeats 11 d1wmxa_ 10.3 19 Fold:Galactose-binding domain-like Superfamily:Galactose-binding domain-like Family:Family 30 carbohydrate binding module, CBM30 (PKD repeat) 12 d2b4wa1 10.1 50 Fold:5-bladed beta-propeller Superfamily:Arabinanase/levansucrase/invertase Family:LmjF10.1260-like 13 c2o6wA_ 9.6 16 PDB header:unknown function Chain: A: PDB Molecule:repeat five residue (rfr) protein or PDBTitle: crystal structure of a pentapeptide repeat protein (rfr23)2 from the cyanobacterium cyanothece 51142 14 c3pssB_ 9.0 14 PDB header:cell cycle Chain: B: PDB Molecule:qnr; PDBTitle: crystal structure of ahqnr, the qnr protein from aeromonas hydrophila2 (p21 crystal form) 15 c2yuiA_ 8.5 21 PDB header:apoptosis Chain: A: PDB Molecule:anamorsin; PDBTitle: solution structure of the n-terminal domain in human2 cytokine-induced apoptosis inhibitor anamorsin 16 c3n90A_ 8.2 23 PDB header:unknown function Chain: A: PDB Molecule:thylakoid lumenal 15 kda protein 1, chloroplastic; PDBTitle: the 1.7 angstrom resolution crystal structure of at2g44920, a2 pentapeptide repeat protein from arabidopsis thaliana thylakoid3 lumen. 17 d2f3la1 8.0 18 Fold:Single-stranded right-handed beta-helix Superfamily:Pentapeptide repeat-like Family:Pentapeptide repeats 18 c2w7zB_ 7.8 15 PDB header:inhibitor Chain: B: PDB Molecule:pentapeptide repeat family protein; PDBTitle: structure of the pentapeptide repeat protein efsqnr, a dna2 gyrase inhibitor. free amines modified by cyclic3 pentylation with glutaraldehyde. 19 d2zjrj1 7.7 26 Fold:alpha/beta-Hammerhead Superfamily:Ribosomal protein L16p/L10e Family:Ribosomal protein L16p 20 d2bm5a1 7.7 12 Fold:Single-stranded right-handed beta-helix Superfamily:Pentapeptide repeat-like Family:Pentapeptide repeats 21 d2coba1 not modelled 7.6 20 Fold:DNA/RNA-binding 3-helical bundle Superfamily:Homeodomain-like Family:Psq domain 22 c2g0yA_ not modelled 7.2 26 PDB header:unknown function Chain: A: PDB Molecule:pentapeptide repeat protein; PDBTitle: crystal structure of a lumenal pentapeptide repeat protein from2 cyanothece sp 51142 at 2.3 angstrom resolution. tetragonal crystal3 form 23 d2j01q1 not modelled 6.9 35 Fold:alpha/beta-Hammerhead Superfamily:Ribosomal protein L16p/L10e Family:Ribosomal protein L16p 24 c3e3uA_ not modelled 6.6 27 PDB header:hydrolase Chain: A: PDB Molecule:peptide deformylase; PDBTitle: crystal structure of mycobacterium tuberculosis peptide2 deformylase in complex with inhibitor 25 c3bboO_ not modelled 6.5 35 PDB header:ribosome Chain: O: PDB Molecule:ribosomal protein l16; PDBTitle: homology model for the spinach chloroplast 50s subunit2 fitted to 9.4a cryo-em map of the 70s chlororibosome 26 d2gyck1 not modelled 6.4 26 Fold:alpha/beta-Hammerhead Superfamily:Ribosomal protein L16p/L10e Family:Ribosomal protein L16p 27 d1iuqa_ not modelled 6.1 21 Fold:Glycerol-3-phosphate (1)-acyltransferase Superfamily:Glycerol-3-phosphate (1)-acyltransferase Family:Glycerol-3-phosphate (1)-acyltransferase 28 c3aqoD_ not modelled 5.6 21 PDB header:membrane protein Chain: D: PDB Molecule:probable secdf protein-export membrane protein; PDBTitle: structure and function of a membrane component secdf that enhances2 protein export 29 c2k3jA_ not modelled 5.6 78 PDB header:oxidoreductase Chain: A: PDB Molecule:mitochondrial intermembrane space import and PDBTitle: the solution structure of human mia40 30 c2jx0A_ not modelled 5.6 20 PDB header:cell adhesion, signaling protein Chain: A: PDB Molecule:arf gtpase-activating protein git1; PDBTitle: the paxillin-binding domain (pbd) of g protein coupled2 receptor (gpcr)-kinase (grk) interacting protein 1 (git1) 31 d1kshb_ not modelled 5.6 38 Fold:Immunoglobulin-like beta-sandwich Superfamily:E set domains Family:RhoGDI-like 32 c3nw8B_ not modelled 5.4 19 PDB header:viral protein Chain: B: PDB Molecule:envelope glycoprotein b; PDBTitle: glycoprotein b from herpes simplex virus type 1, y179s mutant, high-ph

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0