Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	P	H	T	I	K	K	M	S	L	I	G	L	I	L	M	I	F	T	S	V	F	G	F	A	N	S	P	S	A	Y	Y	L	M	G	Y	S	A	I	P	F	Y	I	F	S	A	L	L	F	F	I	P	F	A	L	M	M	A	E	M
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?																			?							?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		G	A	A	Y	R	K	E	E	G	G	I	Y	S	W	M	N	N	S	V	G	P	R	F	A	F	I	G	T	F	M	W	F	S	S	Y	I	I	W	M	V	S	T	S	A	K	V	W	V	P	F	S	T	F	L	Y	G	S	D	M	T
Secondary structure
SS confidence
Disorder			?				?	?																																																				?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		Q	H	W	R	I	A	G	L	E	P	T	Q	V	V	G	L	L	A	V	A	W	M	I	L	V	T	V	V	A	S	K	G	I	N	K	I	A	R	I	T	A	V	G	G	I	A	V	M	C	L	N	L	V	L	L	L	V	S	I	T
Secondary structure
SS confidence
Disorder		?							?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		I	L	L	L	N	G	G	H	F	A	Q	D	I	N	F	L	A	S	P	N	P	G	Y	Q	S	G	L	A	M	L	S	F	V	V	F	A	I	F	A	Y	G	G	I	E	A	V	G	G	L	V	D	K	T	E	N	P	E	K	N	F
Secondary structure
SS confidence
Disorder									?	?	?			?		?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		A	K	G	I	V	F	A	A	I	V	I	S	I	G	Y	S	L	A	I	F	L	W	G	V	S	T	N	W	Q	Q	V	L	S	N	G	S	V	N	L	G	N	I	T	Y	V	L	M	K	S	L	G	M	T	L	G	N	A	L	H	L
Secondary structure
SS confidence
Disorder																												?				?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?		?			?	?	?	?		?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		S	P	E	A	S	L	S	L	G	V	W	F	A	R	I	T	G	L	S	M	F	L	A	Y	T	G	A	F	F	T	L	C	Y	S	P	L	K	A	I	I	Q	G	T	P	K	A	L	W	P	E	P	M	T	R	L	N	A	M	G	M
Secondary structure
SS confidence
Disorder							?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		P	S	I	A	M	W	M	Q	C	G	L	V	T	V	F	I	L	L	V	S	F	G	G	G	T	A	S	A	F	F	N	K	L	T	L	M	A	N	V	S	M	T	L	P	Y	L	F	L	A	L	A	F	P	F	F	K	A	R	Q	D
Secondary structure
SS confidence
Disorder																					?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		L	D	R	P	F	V	I	F	K	T	H	L	S	A	M	I	A	T	V	V	V	V	L	V	V	T	F	A	N	V	F	T	I	I	Q	P	V	V	E	A	G	D	W	D	S	T	L	W	M	I	G	G	P	V	F	F	S	L	L	A
Secondary structure
SS confidence
Disorder																																				?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500
Sequence		M	A	I	Y	Q	N	Y	C	S	R	V	A	K	N	P	Q	W	A	V	E
Secondary structure
SS confidence
Disorder									?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 3gia chain A Region: 5 - 496 Aligned: 425 Modelled: 425 Confidence: 100.0% Identity: 13% PDB header:transport protein Chain: A: PDB Molecule:uncharacterized protein mj0609; PDBTitle: crystal structure of apct transporter Phyre2 PDB 3lrc chain C Region: 4 - 495 Aligned: 408 Modelled: 408 Confidence: 100.0% Identity: 17% PDB header:transport protein Chain: C: PDB Molecule:arginine/agmatine antiporter; PDBTitle: structure of e. coli adic (p1) Phyre2 PDB 2jln chain A Region: 2 - 500 Aligned: 438 Modelled: 438 Confidence: 100.0% Identity: 12% PDB header:membrane protein Chain: A: PDB Molecule:mhp1; PDBTitle: structure of mhp1, a nucleobase-cation-symport-1 family2 transporter Phyre2 PDB 2xq2 chain A Region: 6 - 500 Aligned: 439 Modelled: 439 Confidence: 99.0% Identity: 12% PDB header:transport protein Chain: A: PDB Molecule:sodium/glucose cotransporter; PDBTitle: structure of the k294a mutant of vsglt Phyre2 PDB 3dh4 chain A Region: 6 - 500 Aligned: 450 Modelled: 477 Confidence: 98.7% Identity: 14% PDB header:transport protein Chain: A: PDB Molecule:sodium/glucose cotransporter; PDBTitle: crystal structure of sodium/sugar symporter with bound galactose from2 vibrio parahaemolyticus Phyre2 PDB 2a65 chain A domain 1 Region: 9 - 490 Aligned: 453 Modelled: 453 Confidence: 98.6% Identity: 15% Fold: SNF-like Superfamily: SNF-like Family: SNF-like Phyre2 PDB 2w8a chain C Region: 2 - 426 Aligned: 388 Modelled: 399 Confidence: 96.8% Identity: 12% PDB header:membrane protein Chain: C: PDB Molecule:glycine betaine transporter betp; PDBTitle: crystal structure of the sodium-coupled glycine betaine2 symporter betp from corynebacterium glutamicum with bound3 substrate Phyre2 PDB 3qnq chain D Region: 459 - 500 Aligned: 42 Modelled: 42 Confidence: 80.1% Identity: 2% PDB header:membrane protein, transport protein Chain: D: PDB Molecule:pts system, cellobiose-specific iic component; PDBTitle: crystal structure of the transporter chbc, the iic component from the2 n,n'-diacetylchitobiose-specific phosphotransferase system Phyre2 PDB 1fft chain B domain 2 Region: 385 - 453 Aligned: 69 Modelled: 69 Confidence: 44.4% Identity: 10% Fold: Transmembrane helix hairpin Superfamily: Cytochrome c oxidase subunit II-like, transmembrane region Family: Cytochrome c oxidase subunit II-like, transmembrane region Phyre2 PDB 3hfx chain A Region: 5 - 422 Aligned: 395 Modelled: 395 Confidence: 38.2% Identity: 12% PDB header:transport protein Chain: A: PDB Molecule:l-carnitine/gamma-butyrobetaine antiporter; PDBTitle: crystal structure of carnitine transporter Phyre2 PDB 3rko chain F Region: 358 - 493 Aligned: 130 Modelled: 136 Confidence: 34.1% Identity: 8% PDB header:oxidoreductase Chain: F: PDB Molecule:nadh-quinone oxidoreductase subunit j; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 e. coli at 3.0 angstrom resolution Phyre2 PDB 2knc chain A Region: 463 - 500 Aligned: 38 Modelled: 38 Confidence: 19.5% Identity: 16% PDB header:cell adhesion Chain: A: PDB Molecule:integrin alpha-iib; PDBTitle: platelet integrin alfaiib-beta3 transmembrane-cytoplasmic2 heterocomplex Phyre2 PDB 1zrt chain D Region: 464 - 495 Aligned: 32 Modelled: 32 Confidence: 9.6% Identity: 6% PDB header:oxidoreductase/metal transport Chain: D: PDB Molecule:cytochrome c1; PDBTitle: rhodobacter capsulatus cytochrome bc1 complex with2 stigmatellin bound Phyre2 PDB 3m7b chain A Region: 358 - 496 Aligned: 131 Modelled: 139 Confidence: 7.6% Identity: 8% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:tellurite resistance protein teha homolog; PDBTitle: crystal structure of plant slac1 homolog teha Phyre2 PDB 1m57 chain H Region: 386 - 453 Aligned: 67 Modelled: 68 Confidence: 6.2% Identity: 10% PDB header:oxidoreductase Chain: H: PDB Molecule:cytochrome c oxidase; PDBTitle: structure of cytochrome c oxidase from rhodobacter2 sphaeroides (eq(i-286) mutant)) Phyre2 PDB 3cwb chain Q Region: 467 - 495 Aligned: 29 Modelled: 29 Confidence: 6.1% Identity: 14% PDB header:oxidoreductase Chain: Q: PDB Molecule:mitochondrial cytochrome c1, heme protein; PDBTitle: chicken cytochrome bc1 complex inhibited by an iodinated analogue of2 the polyketide crocacin-d Phyre2 1 c3giaA_ 2 c3lrcC_ 3 c2jlnA_ 4 c2xq2A_ 5 c3dh4A_ 6 d2a65a1 7 c2w8aC_ 8 c3qnqD_ 9 d1fftb2 10 c3hfxA_ 11 c3rkoF_ 12 c2kncA_ 13 c1zrtD_ 14 c3m7bA_ 15 c1m57H_ 16 c3cwbQ_

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c3giaA_ 100.0 13 PDB header:transport protein Chain: A: PDB Molecule:uncharacterized protein mj0609; PDBTitle: crystal structure of apct transporter 2 c3lrcC_ 100.0 17 PDB header:transport protein Chain: C: PDB Molecule:arginine/agmatine antiporter; PDBTitle: structure of e. coli adic (p1) 3 c2jlnA_ 100.0 12 PDB header:membrane protein Chain: A: PDB Molecule:mhp1; PDBTitle: structure of mhp1, a nucleobase-cation-symport-1 family2 transporter 4 c2xq2A_ 99.0 12 PDB header:transport protein Chain: A: PDB Molecule:sodium/glucose cotransporter; PDBTitle: structure of the k294a mutant of vsglt 5 c3dh4A_ 98.7 14 PDB header:transport protein Chain: A: PDB Molecule:sodium/glucose cotransporter; PDBTitle: crystal structure of sodium/sugar symporter with bound galactose from2 vibrio parahaemolyticus 6 d2a65a1 98.6 15 Fold:SNF-like Superfamily:SNF-like Family:SNF-like 7 c2w8aC_ 96.8 12 PDB header:membrane protein Chain: C: PDB Molecule:glycine betaine transporter betp; PDBTitle: crystal structure of the sodium-coupled glycine betaine2 symporter betp from corynebacterium glutamicum with bound3 substrate 8 c3qnqD_ 80.1 2 PDB header:membrane protein, transport protein Chain: D: PDB Molecule:pts system, cellobiose-specific iic component; PDBTitle: crystal structure of the transporter chbc, the iic component from the2 n,n'-diacetylchitobiose-specific phosphotransferase system 9 d1fftb2 44.4 10 Fold:Transmembrane helix hairpin Superfamily:Cytochrome c oxidase subunit II-like, transmembrane region Family:Cytochrome c oxidase subunit II-like, transmembrane region 10 c3hfxA_ 38.2 12 PDB header:transport protein Chain: A: PDB Molecule:l-carnitine/gamma-butyrobetaine antiporter; PDBTitle: crystal structure of carnitine transporter 11 c3rkoF_ 34.1 8 PDB header:oxidoreductase Chain: F: PDB Molecule:nadh-quinone oxidoreductase subunit j; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 e. coli at 3.0 angstrom resolution 12 c2kncA_ 19.5 16 PDB header:cell adhesion Chain: A: PDB Molecule:integrin alpha-iib; PDBTitle: platelet integrin alfaiib-beta3 transmembrane-cytoplasmic2 heterocomplex 13 c1zrtD_ 9.6 6 PDB header:oxidoreductase/metal transport Chain: D: PDB Molecule:cytochrome c1; PDBTitle: rhodobacter capsulatus cytochrome bc1 complex with2 stigmatellin bound 14 c3m7bA_ 7.6 8 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:tellurite resistance protein teha homolog; PDBTitle: crystal structure of plant slac1 homolog teha 15 c1m57H_ 6.2 10 PDB header:oxidoreductase Chain: H: PDB Molecule:cytochrome c oxidase; PDBTitle: structure of cytochrome c oxidase from rhodobacter2 sphaeroides (eq(i-286) mutant)) 16 c3cwbQ_ 6.1 14 PDB header:oxidoreductase Chain: Q: PDB Molecule:mitochondrial cytochrome c1, heme protein; PDBTitle: chicken cytochrome bc1 complex inhibited by an iodinated analogue of2 the polyketide crocacin-d

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0