Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	R	F	I	I	R	T	V	M	L	I	A	L	V	W	I	G	L	L	L	S	G	Y	G	V	L	I	G	S	K	E	N	A	A	G	L	G	L	Q	C	T	Y	L	T	A	R	G	T	S	T	V	Q	Y	L	H	T	K	S	G	F
Secondary structure
SS confidence
Disorder		?	?	?																											?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.
Sequence		L	G	I	T	D	C	P	L	L	R	K	S	N	I	V	V	D	N	G
Secondary structure
SS confidence
Disorder								?						?				?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1w6t chain A domain 2 Region: 38 - 67 Aligned: 30 Modelled: 30 Confidence: 23.9% Identity: 30% Fold: Enolase N-terminal domain-like Superfamily: Enolase N-terminal domain-like Family: Enolase N-terminal domain-like Phyre2 PDB 2fym chain A Region: 41 - 67 Aligned: 27 Modelled: 27 Confidence: 21.2% Identity: 33% PDB header:lyase Chain: A: PDB Molecule:enolase; PDBTitle: crystal structure of e. coli enolase complexed with the2 minimal binding segment of rnase e. Phyre2 PDB 2ivd chain A domain 2 Region: 22 - 40 Aligned: 19 Modelled: 19 Confidence: 20.9% Identity: 32% Fold: FAD-linked reductases, C-terminal domain Superfamily: FAD-linked reductases, C-terminal domain Family: L-aminoacid/polyamine oxidase Phyre2 PDB 2a8v chain A Region: 22 - 32 Aligned: 11 Modelled: 11 Confidence: 14.5% Identity: 27% PDB header:protein/rna Chain: A: PDB Molecule:rna binding domain of rho transcription PDBTitle: rho transcription termination factor/rna complex Phyre2 PDB 1sez chain A domain 2 Region: 20 - 38 Aligned: 19 Modelled: 19 Confidence: 14.2% Identity: 53% Fold: FAD-linked reductases, C-terminal domain Superfamily: FAD-linked reductases, C-terminal domain Family: L-aminoacid/polyamine oxidase Phyre2 PDB 2d13 chain A domain 1 Region: 65 - 79 Aligned: 14 Modelled: 15 Confidence: 13.6% Identity: 36% Fold: Adenine nucleotide alpha hydrolase-like Superfamily: Adenine nucleotide alpha hydrolases-like Family: N-type ATP pyrophosphatases Phyre2 PDB 2ozt chain A Region: 36 - 66 Aligned: 31 Modelled: 31 Confidence: 12.3% Identity: 29% PDB header:lyase Chain: A: PDB Molecule:tlr1174 protein; PDBTitle: crystal structure of o-succinylbenzoate synthase from2 thermosynechococcus elongatus bp-1 Phyre2 PDB 3d4c chain A Region: 30 - 42 Aligned: 13 Modelled: 13 Confidence: 11.6% Identity: 23% PDB header:cell adhesion Chain: A: PDB Molecule:maltose-binding periplasmic protein, linker, zona pellucida PDBTitle: zp-n domain of mammalian sperm receptor zp3 (crystal form i) Phyre2 PDB 3tqp chain A Region: 41 - 67 Aligned: 27 Modelled: 27 Confidence: 11.1% Identity: 22% PDB header:lyase Chain: A: PDB Molecule:enolase; PDBTitle: structure of an enolase (eno) from coxiella burnetii Phyre2 PDB 1a62 chain A domain 2 Region: 22 - 32 Aligned: 11 Modelled: 11 Confidence: 11.0% Identity: 27% Fold: OB-fold Superfamily: Nucleic acid-binding proteins Family: Cold shock DNA-binding domain-like Phyre2 PDB 1wuf chain A domain 2 Region: 36 - 67 Aligned: 32 Modelled: 32 Confidence: 10.6% Identity: 25% Fold: Enolase N-terminal domain-like Superfamily: Enolase N-terminal domain-like Family: Enolase N-terminal domain-like Phyre2 PDB 1ru8 chain A Region: 65 - 79 Aligned: 14 Modelled: 15 Confidence: 10.2% Identity: 50% Fold: Adenine nucleotide alpha hydrolase-like Superfamily: Adenine nucleotide alpha hydrolases-like Family: N-type ATP pyrophosphatases Phyre2 PDB 1muc chain A domain 2 Region: 54 - 67 Aligned: 14 Modelled: 14 Confidence: 9.6% Identity: 21% Fold: Enolase N-terminal domain-like Superfamily: Enolase N-terminal domain-like Family: Enolase N-terminal domain-like Phyre2 PDB 1iyx chain A domain 2 Region: 41 - 67 Aligned: 27 Modelled: 27 Confidence: 9.1% Identity: 33% Fold: Enolase N-terminal domain-like Superfamily: Enolase N-terminal domain-like Family: Enolase N-terminal domain-like Phyre2 PDB 3qn3 chain B Region: 40 - 67 Aligned: 28 Modelled: 28 Confidence: 9.0% Identity: 29% PDB header:lyase Chain: B: PDB Molecule:enolase; PDBTitle: phosphopyruvate hydratase from campylobacter jejuni. Phyre2 PDB 2ezl chain A Region: 15 - 32 Aligned: 15 Modelled: 18 Confidence: 8.8% Identity: 40% Fold: DNA/RNA-binding 3-helical bundle Superfamily: Homeodomain-like Family: Recombinase DNA-binding domain Phyre2 PDB 2mnr chain A domain 2 Region: 54 - 69 Aligned: 16 Modelled: 16 Confidence: 8.3% Identity: 25% Fold: Enolase N-terminal domain-like Superfamily: Enolase N-terminal domain-like Family: Enolase N-terminal domain-like Phyre2 PDB 3tj4 chain B Region: 54 - 69 Aligned: 16 Modelled: 16 Confidence: 8.2% Identity: 19% PDB header:lyase Chain: B: PDB Molecule:mandelate racemase; PDBTitle: crystal structure of an enolase from agrobacterium tumefaciens (efi2 target efi-502087) no mg Phyre2 PDB 1bqg chain A domain 2 Region: 45 - 67 Aligned: 23 Modelled: 23 Confidence: 7.6% Identity: 26% Fold: Enolase N-terminal domain-like Superfamily: Enolase N-terminal domain-like Family: Enolase N-terminal domain-like Phyre2 PDB 1jdf chain A domain 2 Region: 45 - 67 Aligned: 23 Modelled: 23 Confidence: 7.5% Identity: 22% Fold: Enolase N-terminal domain-like Superfamily: Enolase N-terminal domain-like Family: Enolase N-terminal domain-like Phyre2 1 d1w6ta2 2 c2fymA_ 3 d2ivda2 4 c2a8vA_ 5 d1seza2 6 d2d13a1 7 c2oztA_ 8 c3d4cA_ 9 c3tqpA_ 10 d1a62a2 11 d1wufa2 12 d1ru8a_ 13 d1muca2 14 d1iyxa2 15 c3qn3B_ 16 d2ezla_ 17 d2mnra2 18 c3tj4B_ 19 d1bqga2 20 d1jdfa2 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1w6ta2 23.9 30 Fold:Enolase N-terminal domain-like Superfamily:Enolase N-terminal domain-like Family:Enolase N-terminal domain-like 2 c2fymA_ 21.2 33 PDB header:lyase Chain: A: PDB Molecule:enolase; PDBTitle: crystal structure of e. coli enolase complexed with the2 minimal binding segment of rnase e. 3 d2ivda2 20.9 32 Fold:FAD-linked reductases, C-terminal domain Superfamily:FAD-linked reductases, C-terminal domain Family:L-aminoacid/polyamine oxidase 4 c2a8vA_ 14.5 27 PDB header:protein/rna Chain: A: PDB Molecule:rna binding domain of rho transcription PDBTitle: rho transcription termination factor/rna complex 5 d1seza2 14.2 53 Fold:FAD-linked reductases, C-terminal domain Superfamily:FAD-linked reductases, C-terminal domain Family:L-aminoacid/polyamine oxidase 6 d2d13a1 13.6 36 Fold:Adenine nucleotide alpha hydrolase-like Superfamily:Adenine nucleotide alpha hydrolases-like Family:N-type ATP pyrophosphatases 7 c2oztA_ 12.3 29 PDB header:lyase Chain: A: PDB Molecule:tlr1174 protein; PDBTitle: crystal structure of o-succinylbenzoate synthase from2 thermosynechococcus elongatus bp-1 8 c3d4cA_ 11.6 23 PDB header:cell adhesion Chain: A: PDB Molecule:maltose-binding periplasmic protein, linker, zona pellucida PDBTitle: zp-n domain of mammalian sperm receptor zp3 (crystal form i) 9 c3tqpA_ 11.1 22 PDB header:lyase Chain: A: PDB Molecule:enolase; PDBTitle: structure of an enolase (eno) from coxiella burnetii 10 d1a62a2 11.0 27 Fold:OB-fold Superfamily:Nucleic acid-binding proteins Family:Cold shock DNA-binding domain-like 11 d1wufa2 10.6 25 Fold:Enolase N-terminal domain-like Superfamily:Enolase N-terminal domain-like Family:Enolase N-terminal domain-like 12 d1ru8a_ 10.2 50 Fold:Adenine nucleotide alpha hydrolase-like Superfamily:Adenine nucleotide alpha hydrolases-like Family:N-type ATP pyrophosphatases 13 d1muca2 9.6 21 Fold:Enolase N-terminal domain-like Superfamily:Enolase N-terminal domain-like Family:Enolase N-terminal domain-like 14 d1iyxa2 9.1 33 Fold:Enolase N-terminal domain-like Superfamily:Enolase N-terminal domain-like Family:Enolase N-terminal domain-like 15 c3qn3B_ 9.0 29 PDB header:lyase Chain: B: PDB Molecule:enolase; PDBTitle: phosphopyruvate hydratase from campylobacter jejuni. 16 d2ezla_ 8.8 40 Fold:DNA/RNA-binding 3-helical bundle Superfamily:Homeodomain-like Family:Recombinase DNA-binding domain 17 d2mnra2 8.3 25 Fold:Enolase N-terminal domain-like Superfamily:Enolase N-terminal domain-like Family:Enolase N-terminal domain-like 18 c3tj4B_ 8.2 19 PDB header:lyase Chain: B: PDB Molecule:mandelate racemase; PDBTitle: crystal structure of an enolase from agrobacterium tumefaciens (efi2 target efi-502087) no mg 19 d1bqga2 7.6 26 Fold:Enolase N-terminal domain-like Superfamily:Enolase N-terminal domain-like Family:Enolase N-terminal domain-like 20 d1jdfa2 7.5 22 Fold:Enolase N-terminal domain-like Superfamily:Enolase N-terminal domain-like Family:Enolase N-terminal domain-like 21 c2pa6A_ not modelled 7.2 32 PDB header:lyase Chain: A: PDB Molecule:enolase; PDBTitle: crystal structure of mj0232 from methanococcus jannaschii 22 c3uj2C_ not modelled 7.1 26 PDB header:lyase Chain: C: PDB Molecule:enolase 1; PDBTitle: crystal structure of an enolase from anaerostipes caccae (efi target2 efi-502054) with bound mg and sulfate 23 c1iyxA_ not modelled 7.1 36 PDB header:lyase Chain: A: PDB Molecule:enolase; PDBTitle: crystal structure of enolase from enterococcus hirae 24 d2gtlo1 not modelled 7.1 29 Fold:Streptavidin-like Superfamily:Extracellular hemoglobin linker subunit, receptor domain Family:Extracellular hemoglobin linker subunit, receptor domain 25 d1rvka2 not modelled 6.9 21 Fold:Enolase N-terminal domain-like Superfamily:Enolase N-terminal domain-like Family:Enolase N-terminal domain-like 26 d2gtln1 not modelled 6.6 17 Fold:Streptavidin-like Superfamily:Extracellular hemoglobin linker subunit, receptor domain Family:Extracellular hemoglobin linker subunit, receptor domain 27 d1jpma2 not modelled 6.4 29 Fold:Enolase N-terminal domain-like Superfamily:Enolase N-terminal domain-like Family:Enolase N-terminal domain-like 28 d2gl5a2 not modelled 6.1 36 Fold:Enolase N-terminal domain-like Superfamily:Enolase N-terminal domain-like Family:Enolase N-terminal domain-like 29 c1x5vA_ not modelled 6.0 50 PDB header:toxin Chain: A: PDB Molecule:pcfk1; PDBTitle: nmr structure of pcfk1 30 c1ec8B_ not modelled 6.0 29 PDB header:lyase Chain: B: PDB Molecule:glucarate dehydratase; PDBTitle: e. coli glucarate dehydratase bound to product 2,3-2 dihydroxy-5-oxo-hexanedioate 31 c2pozA_ not modelled 5.7 19 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:putative dehydratase; PDBTitle: crystal structure of a putative dehydratase from mesorhizobium loti 32 c3msyC_ not modelled 5.5 23 PDB header:isomerase Chain: C: PDB Molecule:mandelate racemase/muconate lactonizing enzyme; PDBTitle: crystal structure of mandelate racemase/muconate lactonizing enzyme2 from a marine actinobacterium 33 c2hxtA_ not modelled 5.4 6 PDB header:unknown function Chain: A: PDB Molecule:l-fuconate dehydratase; PDBTitle: crystal structure of l-fuconate dehydratase from xanthomonas2 campestris liganded with mg++ and d-erythronohydroxamate 34 c2l35B_ not modelled 5.3 13 PDB header:protein binding Chain: B: PDB Molecule:tyro protein tyrosine kinase-binding protein; PDBTitle: structure of the dap12-nkg2c transmembrane heterotrimer 35 c3sjnB_ not modelled 5.2 25 PDB header:lyase Chain: B: PDB Molecule:mandelate racemase/muconate lactonizing protein; PDBTitle: crystal structure of enolase spea_3858 (target efi-500646) from2 shewanella pealeana with magnesium bound

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0