Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	T	M	T	R	L	K	I	S	K	T	L	L	A	V	M	L	T	S	A	V	A	T	G	S	A	Y	A	E	N	N	A	Q	T	T	N	E	S	A	G	Q	K	V	D	S	S	M	N	K	V	G	N	F	M	D	D	S	A	I	T
Secondary structure
SS confidence
Disorder		?	?	?	?																			?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?			?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		A	K	V	K	A	A	L	V	D	H	D	N	I	K	S	T	D	I	S	V	K	T	D	Q	K	V	V	T	L	S	G	F	V	E	S	Q	A	Q	A	E	E	A	V	K	V	A	K	G	V	E	G	V	T	S	V	S	D	K	L	H
Secondary structure
SS confidence
Disorder												?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		V	R	D	A	K	E	G	S	V	K	G	Y	A	G	D	T	A	T	T	S	E	I	K	A	K	L	L	A	D	D	I	V	P	S	R	H	V	K	V	E	T	T	D	G	V	V	Q	L	S	G	T	V	D	S	Q	A	Q	S	D	R
Secondary structure
SS confidence
Disorder					?		?	?	?	?	?	?			?	?	?														?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.
Sequence		A	E	S	I	A	K	A	V	D	G	V	K	S	V	K	N	D	L	K	T	K
Secondary structure
SS confidence
Disorder																						?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2kgs chain A Region: 71 - 200 Aligned: 118 Modelled: 130 Confidence: 98.1% Identity: 22% PDB header:membrane protein Chain: A: PDB Molecule:uncharacterized protein rv0899/mt0922; PDBTitle: solution structure of the amino-terminal domain of ompatb, a pore2 forming protein from mycobacterium tuberculosis Phyre2 PDB 1fe0 chain A Region: 60 - 104 Aligned: 42 Modelled: 45 Confidence: 62.7% Identity: 12% Fold: Ferredoxin-like Superfamily: HMA, heavy metal-associated domain Family: HMA, heavy metal-associated domain Phyre2 PDB 2crl chain A Region: 60 - 105 Aligned: 44 Modelled: 46 Confidence: 53.8% Identity: 16% PDB header:chaperone Chain: A: PDB Molecule:copper chaperone for superoxide dismutase; PDBTitle: the apo form of hma domain of copper chaperone for2 superoxide dismutase Phyre2 PDB 3rv2 chain B Region: 58 - 109 Aligned: 52 Modelled: 52 Confidence: 49.3% Identity: 19% PDB header:transferase Chain: B: PDB Molecule:s-adenosylmethionine synthase; PDBTitle: crystal structure of s-adenosylmethionine synthetase from2 mycobacterium marinum Phyre2 PDB 1qup chain A domain 2 Region: 60 - 104 Aligned: 43 Modelled: 45 Confidence: 47.3% Identity: 14% Fold: Ferredoxin-like Superfamily: HMA, heavy metal-associated domain Family: HMA, heavy metal-associated domain Phyre2 PDB 2qfi chain B Region: 89 - 160 Aligned: 71 Modelled: 72 Confidence: 41.7% Identity: 13% PDB header:transport protein Chain: B: PDB Molecule:ferrous-iron efflux pump fief; PDBTitle: structure of the zinc transporter yiip Phyre2 PDB 1mxa chain A domain 1 Region: 136 - 174 Aligned: 39 Modelled: 39 Confidence: 39.9% Identity: 13% Fold: S-adenosylmethionine synthetase Superfamily: S-adenosylmethionine synthetase Family: S-adenosylmethionine synthetase Phyre2 PDB 1qm4 chain A domain 1 Region: 136 - 174 Aligned: 39 Modelled: 39 Confidence: 38.6% Identity: 18% Fold: S-adenosylmethionine synthetase Superfamily: S-adenosylmethionine synthetase Family: S-adenosylmethionine synthetase Phyre2 PDB 2rog chain A Region: 60 - 105 Aligned: 44 Modelled: 46 Confidence: 38.5% Identity: 14% PDB header:metal binding protein Chain: A: PDB Molecule:heavy metal binding protein; PDBTitle: solution structure of thermus thermophilus hb8 ttha17182 protein in living e. coli cells Phyre2 PDB 1qup chain A Region: 60 - 104 Aligned: 43 Modelled: 45 Confidence: 36.3% Identity: 14% PDB header:chaperone Chain: A: PDB Molecule:superoxide dismutase 1 copper chaperone; PDBTitle: crystal structure of the copper chaperone for superoxide2 dismutase Phyre2 PDB 2p02 chain A domain 1 Region: 136 - 174 Aligned: 39 Modelled: 39 Confidence: 34.5% Identity: 15% Fold: S-adenosylmethionine synthetase Superfamily: S-adenosylmethionine synthetase Family: S-adenosylmethionine synthetase Phyre2 PDB 1cc8 chain A Region: 60 - 105 Aligned: 44 Modelled: 46 Confidence: 33.2% Identity: 14% Fold: Ferredoxin-like Superfamily: HMA, heavy metal-associated domain Family: HMA, heavy metal-associated domain Phyre2 PDB 2klr chain A Region: 71 - 92 Aligned: 22 Modelled: 22 Confidence: 32.5% Identity: 18% PDB header:chaperone Chain: A: PDB Molecule:alpha-crystallin b chain; PDBTitle: solid-state nmr structure of the alpha-crystallin domain in alphab-2 crystallin oligomers Phyre2 PDB 1jk9 chain D Region: 60 - 104 Aligned: 43 Modelled: 45 Confidence: 28.3% Identity: 14% PDB header:oxidoreductase Chain: D: PDB Molecule:copper chaperone for superoxide dismutase; PDBTitle: heterodimer between h48f-ysod1 and yccs Phyre2 PDB 2k2p chain A Region: 60 - 105 Aligned: 44 Modelled: 46 Confidence: 27.2% Identity: 14% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein atu1203; PDBTitle: solution nmr structure of protein atu1203 from agrobacterium2 tumefaciens. northeast structural genomics consortium (nesg) target3 att10, ontario center for structural proteomics target atc1183 Phyre2 PDB 2wj7 chain D Region: 71 - 92 Aligned: 22 Modelled: 22 Confidence: 27.1% Identity: 18% PDB header:chaperone Chain: D: PDB Molecule:alpha-crystallin b chain; PDBTitle: human alphab crystallin Phyre2 PDB 3q9q chain B Region: 71 - 92 Aligned: 22 Modelled: 22 Confidence: 26.4% Identity: 27% PDB header:chaperone Chain: B: PDB Molecule:heat shock protein beta-1; PDBTitle: hspb1 fragment second crystal form Phyre2 PDB 3ibw chain A Region: 59 - 116 Aligned: 57 Modelled: 57 Confidence: 25.9% Identity: 16% PDB header:transferase Chain: A: PDB Molecule:gtp pyrophosphokinase; PDBTitle: crystal structure of the act domain from gtp2 pyrophosphokinase of chlorobium tepidum. northeast3 structural genomics consortium target ctr148a Phyre2 PDB 2l26 chain A Region: 73 - 200 Aligned: 117 Modelled: 128 Confidence: 25.0% Identity: 20% PDB header:membrane protein Chain: A: PDB Molecule:uncharacterized protein rv0899/mt0922; PDBTitle: rv0899 from mycobacterium tuberculosis contains two separated domains Phyre2 PDB 1sb6 chain A Region: 61 - 105 Aligned: 43 Modelled: 45 Confidence: 23.7% Identity: 16% Fold: Ferredoxin-like Superfamily: HMA, heavy metal-associated domain Family: HMA, heavy metal-associated domain Phyre2 1 c2kgsA_ 2 d1fe0a_ 3 c2crlA_ 4 c3rv2B_ 5 d1qupa2 6 c2qfiB_ 7 d1mxaa1 8 d1qm4a1 9 c2rogA_ 10 c1qupA_ 11 d2p02a1 12 d1cc8a_ 13 c2klrA_ 14 c1jk9D_ 15 c2k2pA_ 16 c2wj7D_ 17 c3q9qB_ 18 c3ibwA_ 19 c2l26A_ 20 d1sb6a_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c2kgsA_ 98.1 22 PDB header:membrane protein Chain: A: PDB Molecule:uncharacterized protein rv0899/mt0922; PDBTitle: solution structure of the amino-terminal domain of ompatb, a pore2 forming protein from mycobacterium tuberculosis 2 d1fe0a_ 62.7 12 Fold:Ferredoxin-like Superfamily:HMA, heavy metal-associated domain Family:HMA, heavy metal-associated domain 3 c2crlA_ 53.8 16 PDB header:chaperone Chain: A: PDB Molecule:copper chaperone for superoxide dismutase; PDBTitle: the apo form of hma domain of copper chaperone for2 superoxide dismutase 4 c3rv2B_ 49.3 19 PDB header:transferase Chain: B: PDB Molecule:s-adenosylmethionine synthase; PDBTitle: crystal structure of s-adenosylmethionine synthetase from2 mycobacterium marinum 5 d1qupa2 47.3 14 Fold:Ferredoxin-like Superfamily:HMA, heavy metal-associated domain Family:HMA, heavy metal-associated domain 6 c2qfiB_ 41.7 13 PDB header:transport protein Chain: B: PDB Molecule:ferrous-iron efflux pump fief; PDBTitle: structure of the zinc transporter yiip 7 d1mxaa1 39.9 13 Fold:S-adenosylmethionine synthetase Superfamily:S-adenosylmethionine synthetase Family:S-adenosylmethionine synthetase 8 d1qm4a1 38.6 18 Fold:S-adenosylmethionine synthetase Superfamily:S-adenosylmethionine synthetase Family:S-adenosylmethionine synthetase 9 c2rogA_ 38.5 14 PDB header:metal binding protein Chain: A: PDB Molecule:heavy metal binding protein; PDBTitle: solution structure of thermus thermophilus hb8 ttha17182 protein in living e. coli cells 10 c1qupA_ 36.3 14 PDB header:chaperone Chain: A: PDB Molecule:superoxide dismutase 1 copper chaperone; PDBTitle: crystal structure of the copper chaperone for superoxide2 dismutase 11 d2p02a1 34.5 15 Fold:S-adenosylmethionine synthetase Superfamily:S-adenosylmethionine synthetase Family:S-adenosylmethionine synthetase 12 d1cc8a_ 33.2 14 Fold:Ferredoxin-like Superfamily:HMA, heavy metal-associated domain Family:HMA, heavy metal-associated domain 13 c2klrA_ 32.5 18 PDB header:chaperone Chain: A: PDB Molecule:alpha-crystallin b chain; PDBTitle: solid-state nmr structure of the alpha-crystallin domain in alphab-2 crystallin oligomers 14 c1jk9D_ 28.3 14 PDB header:oxidoreductase Chain: D: PDB Molecule:copper chaperone for superoxide dismutase; PDBTitle: heterodimer between h48f-ysod1 and yccs 15 c2k2pA_ 27.2 14 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein atu1203; PDBTitle: solution nmr structure of protein atu1203 from agrobacterium2 tumefaciens. northeast structural genomics consortium (nesg) target3 att10, ontario center for structural proteomics target atc1183 16 c2wj7D_ 27.1 18 PDB header:chaperone Chain: D: PDB Molecule:alpha-crystallin b chain; PDBTitle: human alphab crystallin 17 c3q9qB_ 26.4 27 PDB header:chaperone Chain: B: PDB Molecule:heat shock protein beta-1; PDBTitle: hspb1 fragment second crystal form 18 c3ibwA_ 25.9 16 PDB header:transferase Chain: A: PDB Molecule:gtp pyrophosphokinase; PDBTitle: crystal structure of the act domain from gtp2 pyrophosphokinase of chlorobium tepidum. northeast3 structural genomics consortium target ctr148a 19 c2l26A_ 25.0 20 PDB header:membrane protein Chain: A: PDB Molecule:uncharacterized protein rv0899/mt0922; PDBTitle: rv0899 from mycobacterium tuberculosis contains two separated domains 20 d1sb6a_ 23.7 16 Fold:Ferredoxin-like Superfamily:HMA, heavy metal-associated domain Family:HMA, heavy metal-associated domain 21 c2zztA_ not modelled 23.5 9 PDB header:transport protein Chain: A: PDB Molecule:putative uncharacterized protein; PDBTitle: crystal structure of the cytosolic domain of the cation2 diffusion facilitator family protein 22 d2cu6a1 not modelled 23.1 13 Fold:Alpha-lytic protease prodomain-like Superfamily:Fe-S cluster assembly (FSCA) domain-like Family:PaaD-like 23 d2qfia1 not modelled 20.6 17 Fold:Alpha-lytic protease prodomain-like Superfamily:Cation efflux protein cytoplasmic domain-like Family:Cation efflux protein cytoplasmic domain-like 24 c2wj5A_ not modelled 16.8 23 PDB header:chaperone Chain: A: PDB Molecule:heat shock protein beta-6; PDBTitle: rat alpha crystallin domain 25 c3imlB_ not modelled 15.7 21 PDB header:transferase Chain: B: PDB Molecule:s-adenosylmethionine synthetase; PDBTitle: crystal structure of s-adenosylmethionine synthetase from burkholderia2 pseudomallei 26 c3glaA_ not modelled 15.7 22 PDB header:chaperone Chain: A: PDB Molecule:low molecular weight heat shock protein; PDBTitle: crystal structure of the hspa from xanthomonas axonopodis 27 c1rg9D_ not modelled 15.0 13 PDB header:transferase Chain: D: PDB Molecule:s-adenosylmethionine synthetase; PDBTitle: s-adenosylmethionine synthetase complexed with sam and ppnp 28 c3l1eA_ not modelled 14.6 23 PDB header:chaperone Chain: A: PDB Molecule:alpha-crystallin a chain; PDBTitle: bovine alphaa crystallin zinc bound 29 c3so4C_ not modelled 13.6 18 PDB header:transferase Chain: C: PDB Molecule:methionine-adenosyltransferase; PDBTitle: methionine-adenosyltransferase from entamoeba histolytica 30 c2obvA_ not modelled 13.1 18 PDB header:transferase Chain: A: PDB Molecule:s-adenosylmethionine synthetase isoform type-1; PDBTitle: crystal structure of the human s-adenosylmethionine synthetase 1 in2 complex with the product 31 d1xhja_ not modelled 11.3 14 Fold:Alpha-lytic protease prodomain-like Superfamily:Fe-S cluster assembly (FSCA) domain-like Family:NifU C-terminal domain-like 32 c2x36F_ not modelled 10.0 13 PDB header:hydrolase Chain: F: PDB Molecule:lon protease homolog, mitochondrial; PDBTitle: structure of the proteolytic domain of the human2 mitochondrial lon protease 33 c3lnoA_ not modelled 9.5 17 PDB header:unknown function Chain: A: PDB Molecule:putative uncharacterized protein; PDBTitle: crystal structure of domain of unknown function duf59 from2 bacillus anthracis 34 d1gpma3 not modelled 9.4 11 Fold:Alpha-lytic protease prodomain-like Superfamily:GMP synthetase C-terminal dimerisation domain Family:GMP synthetase C-terminal dimerisation domain 35 d1uwda_ not modelled 9.3 20 Fold:Alpha-lytic protease prodomain-like Superfamily:Fe-S cluster assembly (FSCA) domain-like Family:PaaD-like 36 d2joqa1 not modelled 9.2 14 Fold:Ferredoxin-like Superfamily:YbeD/HP0495-like Family:HP0495-like 37 d1rwua_ not modelled 9.0 17 Fold:Ferredoxin-like Superfamily:YbeD/HP0495-like Family:YbeD-like 38 c1rwuA_ not modelled 9.0 17 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical upf0250 protein ybed; PDBTitle: solution structure of conserved protein ybed from e. coli 39 c2bolA_ not modelled 8.6 17 PDB header:heat shock protein Chain: A: PDB Molecule:small heat shock protein; PDBTitle: crystal structure and assembly of tsp36, a metazoan small2 heat shock protein 40 d2ffma1 not modelled 8.4 21 Fold:Hypothetical protein SAV1430 Superfamily:Hypothetical protein SAV1430 Family:Hypothetical protein SAV1430 41 c2e3uA_ not modelled 8.4 15 PDB header:rna binding protein Chain: A: PDB Molecule:hypothetical protein ph1566; PDBTitle: crystal structure analysis of dim2p from pyrococcus horikoshii ot3 42 c2k1hA_ not modelled 7.7 15 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein ser13; PDBTitle: solution nmr structure of ser13 from staphylococcus epidermidis.2 northeast structural genomics consortium target ser13 43 d2fmra_ not modelled 7.6 29 Fold:Eukaryotic type KH-domain (KH-domain type I) Superfamily:Eukaryotic type KH-domain (KH-domain type I) Family:Eukaryotic type KH-domain (KH-domain type I) 44 d1m0sa2 not modelled 7.5 27 Fold:Ferredoxin-like Superfamily:D-ribose-5-phosphate isomerase (RpiA), lid domain Family:D-ribose-5-phosphate isomerase (RpiA), lid domain 45 c2ropA_ not modelled 6.1 11 PDB header:hydrolase Chain: A: PDB Molecule:copper-transporting atpase 2; PDBTitle: solution structure of domains 3 and 4 of human atp7b 46 d1vyia_ not modelled 6.1 23 Fold:Phosphoprotein M1, C-terminal domain Superfamily:Phosphoprotein M1, C-terminal domain Family:Phosphoprotein M1, C-terminal domain 47 c3fveA_ not modelled 5.9 38 PDB header:isomerase Chain: A: PDB Molecule:diaminopimelate epimerase; PDBTitle: crystal structure of diaminopimelate epimerase mycobacterium2 tuberculosis dapf 48 d1iloa_ not modelled 5.9 14 Fold:Thioredoxin fold Superfamily:Thioredoxin-like Family:Thioltransferase 49 d1shsa_ not modelled 5.8 9 Fold:HSP20-like chaperones Superfamily:HSP20-like chaperones Family:HSP20 50 c1shsD_ not modelled 5.8 9 PDB header:heat shock protein Chain: D: PDB Molecule:small heat shock protein; PDBTitle: small heat shock protein from methanococcus jannaschii

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

---

Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0