Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	T	E	I	V	A	D	K	T	V	E	V	V	K	N	A	I	E	T	A	D	G	A	L	D	L	Y	N	K	Y	L	D	Q	V	I	P	W	Q	T	F	D	E	T	I	K	E	L	S	R	F	K	Q	E	Y	S	Q	A	A	S	V
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?																																															?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		L	V	G	D	I	K	T	L	L	M	D	S	Q	D	K	Y	F	E	A	T	Q	T	V	Y	E	W	C	G	V	A	T	Q	L	L	A	A	Y	I	L	L	F	D	E	Y	N	E	K	K	A	S	A	Q	K	D	I	L	I	K	V	L
Secondary structure
SS confidence
Disorder													?																																?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		D	D	G	I	T	K	L	N	E	A	Q	K	S	L	L	V	S	S	Q	S	F	N	N	A	S	G	K	L	L	A	L	D	S	Q	L	T	N	D	F	S	E	K	S	S	Y	F	Q	S	Q	V	D	K	I	R	K	E	A	Y	A	G
Secondary structure
SS confidence
Disorder			?		?																															?	?	?	?					?	?									?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		A	A	A	G	V	V	A	G	P	F	G	L	I	I	S	Y	S	I	A	A	G	V	V	E	G	K	L	I	P	E	L	K	N	K	L	K	S	V	Q	N	F	F	T	T	L	S	N	T	V	K	Q	A	N	K	D	I	D	A	A	K
Secondary structure
SS confidence
Disorder																				?		?	?																														?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		L	K	L	T	T	E	I	A	A	I	G	E	I	K	T	E	T	E	T	T	R	F	Y	V	D	Y	D	D	L	M	L	S	L	L	K	E	A	A	K	K	M	I	N	T	C	N	E	Y	Q	K	R	H	G	K	K	T	L	F	E	V
Secondary structure
SS confidence
Disorder																																																										?		?	?
Disorder confidence
		.	.	.
Sequence		P	E	V
Secondary structure
SS confidence
Disorder		?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1qoy chain A Region: 1 - 298 Aligned: 298 Modelled: 298 Confidence: 100.0% Identity: 99% PDB header:toxin Chain: A: PDB Molecule:hemolysin e; PDBTitle: e.coli hemolysin e (hlye, clya, shea) Phyre2 PDB 3brv chain B Region: 261 - 291 Aligned: 31 Modelled: 31 Confidence: 20.7% Identity: 6% PDB header:transferase/transcription Chain: B: PDB Molecule:nf-kappa-b essential modulator; PDBTitle: nemo/ikkb association domain structure Phyre2 PDB 1pca chain A domain 1 Region: 5 - 57 Aligned: 44 Modelled: 53 Confidence: 17.1% Identity: 14% Fold: Ferredoxin-like Superfamily: Protease propeptides/inhibitors Family: Pancreatic carboxypeptidase, activation domain Phyre2 PDB 2cob chain A domain 1 Region: 38 - 60 Aligned: 23 Modelled: 23 Confidence: 15.3% Identity: 26% Fold: DNA/RNA-binding 3-helical bundle Superfamily: Homeodomain-like Family: Psq domain Phyre2 PDB 1r11 chain A domain 3 Region: 83 - 89 Aligned: 7 Modelled: 7 Confidence: 14.0% Identity: 29% Fold: MutS N-terminal domain-like Superfamily: tRNA-intron endonuclease N-terminal domain-like Family: tRNA-intron endonuclease N-terminal domain-like Phyre2 PDB 3pja chain K Region: 82 - 173 Aligned: 91 Modelled: 92 Confidence: 13.8% Identity: 15% PDB header:hydrolase Chain: K: PDB Molecule:translin-associated protein x; PDBTitle: crystal structure of human c3po complex Phyre2 PDB 2q1k chain A Region: 141 - 176 Aligned: 36 Modelled: 36 Confidence: 12.6% Identity: 14% PDB header:chaperone Chain: A: PDB Molecule:asce; PDBTitle: cyrstal structure of asce from aeromonas hydrophilla Phyre2 PDB 1f6f chain A Region: 132 - 267 Aligned: 130 Modelled: 136 Confidence: 12.4% Identity: 10% Fold: 4-helical cytokines Superfamily: 4-helical cytokines Family: Long-chain cytokines Phyre2 PDB 1pfv chain A domain 1 Region: 17 - 99 Aligned: 83 Modelled: 83 Confidence: 12.0% Identity: 16% Fold: Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases Superfamily: Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases Family: Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases Phyre2 PDB 4a9z chain D Region: 124 - 146 Aligned: 23 Modelled: 23 Confidence: 11.2% Identity: 13% PDB header:transcription Chain: D: PDB Molecule:tumor protein 63; PDBTitle: crystal structure of human p63 tetramerization domain Phyre2 PDB 1rqg chain A domain 1 Region: 18 - 99 Aligned: 82 Modelled: 82 Confidence: 10.5% Identity: 16% Fold: Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases Superfamily: Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases Family: Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases Phyre2 PDB 1gg2 chain G Region: 58 - 87 Aligned: 30 Modelled: 30 Confidence: 10.3% Identity: 23% Fold: Non-globular all-alpha subunits of globular proteins Superfamily: Transducin (heterotrimeric G protein), gamma chain Family: Transducin (heterotrimeric G protein), gamma chain Phyre2 PDB 3f1i chain H Region: 85 - 151 Aligned: 67 Modelled: 67 Confidence: 10.1% Identity: 13% PDB header:protein binding Chain: H: PDB Molecule:hepatocyte growth factor-regulated tyrosine kinase PDBTitle: human escrt-0 core complex Phyre2 PDB 1h3n chain A domain 1 Region: 37 - 103 Aligned: 66 Modelled: 67 Confidence: 10.1% Identity: 14% Fold: Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases Superfamily: Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases Family: Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases Phyre2 PDB 1rw5 chain A domain 1 Region: 132 - 229 Aligned: 98 Modelled: 98 Confidence: 9.9% Identity: 7% Fold: 4-helical cytokines Superfamily: 4-helical cytokines Family: Long-chain cytokines Phyre2 PDB 3zy1 chain A Region: 124 - 146 Aligned: 23 Modelled: 23 Confidence: 9.9% Identity: 13% PDB header:transcription Chain: A: PDB Molecule:tumor protein 63; PDBTitle: crystal structure of the human p63 tetramerization domain Phyre2 PDB 3kwl chain A Region: 104 - 167 Aligned: 62 Modelled: 64 Confidence: 9.4% Identity: 18% PDB header:unknown function Chain: A: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of a hypothetical protein from helicobacter pylori Phyre2 PDB 1cii chain A Region: 158 - 205 Aligned: 48 Modelled: 48 Confidence: 9.4% Identity: 13% PDB header:transmembrane protein Chain: A: PDB Molecule:colicin ia; PDBTitle: colicin ia Phyre2 PDB 2ql2 chain A Region: 274 - 286 Aligned: 13 Modelled: 13 Confidence: 8.9% Identity: 23% PDB header:transcription/dna Chain: A: PDB Molecule:transcription factor e2-alpha; PDBTitle: crystal structure of the basic-helix-loop-helix domains of2 the heterodimer e47/neurod1 bound to dna Phyre2 PDB 2ezw chain A domain 1 Region: 283 - 293 Aligned: 11 Modelled: 11 Confidence: 8.9% Identity: 27% Fold: Dimerization-anchoring domain of cAMP-dependent PK regulatory subunit Superfamily: Dimerization-anchoring domain of cAMP-dependent PK regulatory subunit Family: Dimerization-anchoring domain of cAMP-dependent PK regulatory subunit Phyre2 1 c1qoyA_ 2 c3brvB_ 3 d1pcaa1 4 d2coba1 5 d1r11a3 6 c3pjaK_ 7 c2q1kA_ 8 d1f6fa_ 9 d1pfva1 10 c4a9zD_ 11 d1rqga1 12 d1gg2g_ 13 c3f1iH_ 14 d1h3na1 15 d1rw5a1 16 c3zy1A_ 17 c3kwlA_ 18 c1ciiA_ 19 c2ql2A_ 20 d2ezwa1 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c1qoyA_ 100.0 99 PDB header:toxin Chain: A: PDB Molecule:hemolysin e; PDBTitle: e.coli hemolysin e (hlye, clya, shea) 2 c3brvB_ 20.7 6 PDB header:transferase/transcription Chain: B: PDB Molecule:nf-kappa-b essential modulator; PDBTitle: nemo/ikkb association domain structure 3 d1pcaa1 17.1 14 Fold:Ferredoxin-like Superfamily:Protease propeptides/inhibitors Family:Pancreatic carboxypeptidase, activation domain 4 d2coba1 15.3 26 Fold:DNA/RNA-binding 3-helical bundle Superfamily:Homeodomain-like Family:Psq domain 5 d1r11a3 14.0 29 Fold:MutS N-terminal domain-like Superfamily:tRNA-intron endonuclease N-terminal domain-like Family:tRNA-intron endonuclease N-terminal domain-like 6 c3pjaK_ 13.8 15 PDB header:hydrolase Chain: K: PDB Molecule:translin-associated protein x; PDBTitle: crystal structure of human c3po complex 7 c2q1kA_ 12.6 14 PDB header:chaperone Chain: A: PDB Molecule:asce; PDBTitle: cyrstal structure of asce from aeromonas hydrophilla 8 d1f6fa_ 12.4 10 Fold:4-helical cytokines Superfamily:4-helical cytokines Family:Long-chain cytokines 9 d1pfva1 12.0 16 Fold:Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases Superfamily:Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases Family:Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases 10 c4a9zD_ 11.2 13 PDB header:transcription Chain: D: PDB Molecule:tumor protein 63; PDBTitle: crystal structure of human p63 tetramerization domain 11 d1rqga1 10.5 16 Fold:Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases Superfamily:Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases Family:Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases 12 d1gg2g_ 10.3 23 Fold:Non-globular all-alpha subunits of globular proteins Superfamily:Transducin (heterotrimeric G protein), gamma chain Family:Transducin (heterotrimeric G protein), gamma chain 13 c3f1iH_ 10.1 13 PDB header:protein binding Chain: H: PDB Molecule:hepatocyte growth factor-regulated tyrosine kinase PDBTitle: human escrt-0 core complex 14 d1h3na1 10.1 14 Fold:Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases Superfamily:Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases Family:Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases 15 d1rw5a1 9.9 7 Fold:4-helical cytokines Superfamily:4-helical cytokines Family:Long-chain cytokines 16 c3zy1A_ 9.9 13 PDB header:transcription Chain: A: PDB Molecule:tumor protein 63; PDBTitle: crystal structure of the human p63 tetramerization domain 17 c3kwlA_ 9.4 18 PDB header:unknown function Chain: A: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of a hypothetical protein from helicobacter pylori 18 c1ciiA_ 9.4 13 PDB header:transmembrane protein Chain: A: PDB Molecule:colicin ia; PDBTitle: colicin ia 19 c2ql2A_ 8.9 23 PDB header:transcription/dna Chain: A: PDB Molecule:transcription factor e2-alpha; PDBTitle: crystal structure of the basic-helix-loop-helix domains of2 the heterodimer e47/neurod1 bound to dna 20 d2ezwa1 8.9 27 Fold:Dimerization-anchoring domain of cAMP-dependent PK regulatory subunit Superfamily:Dimerization-anchoring domain of cAMP-dependent PK regulatory subunit Family:Dimerization-anchoring domain of cAMP-dependent PK regulatory subunit 21 d1b0na1 not modelled 8.5 12 Fold:Dimerisation interlock Superfamily:SinR repressor dimerisation domain-like Family:SinR repressor dimerisation domain-like 22 d1omwg_ not modelled 8.4 17 Fold:Non-globular all-alpha subunits of globular proteins Superfamily:Transducin (heterotrimeric G protein), gamma chain Family:Transducin (heterotrimeric G protein), gamma chain 23 c2k29A_ not modelled 8.1 24 PDB header:transcription Chain: A: PDB Molecule:antitoxin relb; PDBTitle: structure of the dbd domain of e. coli antitoxin relb 24 d1ggpa_ not modelled 7.7 16 Fold:Ribosome inactivating proteins (RIP) Superfamily:Ribosome inactivating proteins (RIP) Family:Plant cytotoxins 25 d1gotg_ not modelled 7.2 9 Fold:Non-globular all-alpha subunits of globular proteins Superfamily:Transducin (heterotrimeric G protein), gamma chain Family:Transducin (heterotrimeric G protein), gamma chain 26 d1xppa_ not modelled 7.2 8 Fold:DCoH-like Superfamily:RBP11-like subunits of RNA polymerase Family:RBP11/RpoL 27 d1tbge_ not modelled 6.3 9 Fold:Non-globular all-alpha subunits of globular proteins Superfamily:Transducin (heterotrimeric G protein), gamma chain Family:Transducin (heterotrimeric G protein), gamma chain 28 c1gaxB_ not modelled 6.2 15 PDB header:ligase/rna Chain: B: PDB Molecule:valyl-trna synthetase; PDBTitle: crystal structure of thermus thermophilus valyl-trna2 synthetase complexed with trna(val) and valyl-adenylate3 analogue 29 c2zwmA_ not modelled 6.1 14 PDB header:transcription Chain: A: PDB Molecule:transcriptional regulatory protein yycf; PDBTitle: crystal structure of yycf receiver domain from bacillus2 subtilis 30 c2rlwA_ not modelled 5.9 16 PDB header:toxin Chain: A: PDB Molecule:plnf; PDBTitle: three-dimensional structure of the two peptides that2 constitute the two-peptide bacteriocin plantaracin ef 31 c3qqwC_ not modelled 5.9 19 PDB header:lyase Chain: C: PDB Molecule:putative citrate lyase; PDBTitle: crystal structure of a hypothetical lyase (reut_b4148) from ralstonia2 eutropha jmp134 at 2.44 a resolution 32 c3hisA_ not modelled 5.9 11 PDB header:hydrolase Chain: A: PDB Molecule:vacuolar saporin; PDBTitle: crystal structure of saporin-l1 from saponaria officinalis 33 c3df8A_ not modelled 5.8 12 PDB header:transcription Chain: A: PDB Molecule:possible hxlr family transcriptional factor; PDBTitle: the crystal structure of a possible hxlr family transcriptional factor2 from thermoplasma volcanium gss1 34 c2xzmV_ not modelled 5.7 20 PDB header:ribosome Chain: V: PDB Molecule:rps17e; PDBTitle: crystal structure of the eukaryotic 40s ribosomal2 subunit in complex with initiation factor 1. this file3 contains the 40s subunit and initiation factor for4 molecule 1 35 d1vcsa1 not modelled 5.6 14 Fold:STAT-like Superfamily:t-snare proteins Family:t-snare proteins 36 c1jsdB_ not modelled 5.5 23 PDB header:viral protein Chain: B: PDB Molecule:haemagglutinin (ha2 chain); PDBTitle: crystal structure of swine h9 haemagglutinin 37 c1lt1G_ not modelled 5.4 11 PDB header:de novo protein Chain: G: PDB Molecule:l13g-df1; PDBTitle: sliding helix induced change of coordination geometry in a2 model di-mn(ii) protein 38 c3i5pA_ not modelled 5.4 18 PDB header:protein transport Chain: A: PDB Molecule:nucleoporin nup170; PDBTitle: nup170(aa979-1502), s.cerevisiae 39 c2uwjE_ not modelled 5.3 11 PDB header:chaperone Chain: E: PDB Molecule:type iii export protein psce; PDBTitle: structure of the heterotrimeric complex which regulates2 type iii secretion needle formation 40 d1d9ca_ not modelled 5.3 16 Fold:4-helical cytokines Superfamily:4-helical cytokines Family:Interferons/interleukin-10 (IL-10) 41 d1twfk_ not modelled 5.1 16 Fold:DCoH-like Superfamily:RBP11-like subunits of RNA polymerase Family:RBP11/RpoL

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0