PHYRE2 Protein Fold Recognition Server

Standard Mode for job manager, batch processing, Phyre alarm and other advanced options

Retrieve Phyre Job Id

Subscribe to Phyre at Google Groups

Email:

Visit Phyre at Google Groups

Follow @Phyre2server

Protein Homology/analogY Recognition Engine V 2.2

gives you access to our Expert Mode features. Info icon

READ THIS FIRST We are replacing the current Phyre2 server from Thursday 25th to Friday 26th June 2026. While jobs that are submitted might run, we cannot guarantee this and any results could be lost.

If you submit a large batch and many of the individual jobs fail with an "ERROR", please let me know and I will do my best to rescue the situation so that you do not need to resubmit the failed jobs.

We recommend not submitting any jobs that you are not prepared to run again later.

Large "batch" jobs (> 100 jobs) are those that are most likely to be affected.
If you get the web-page that shows that your normal, intensive or one-to-one threading mode job has started, it will probably run successfully, but while we are replacing the server you will not have access to the results

When the server is down, there will be no access to any results

One-to-One Threading Info icon

(which models your sequence against a user-supplied model) can now use models directly from the AlphaFold Protein Structure Database Info icon

Please do not use "intensive mode" unless your search using "normal mode" indicates that a single model does not cover most of your sequence. Info icon

Current Phyre2 server load = 3% (normal running) Info icon

E-mail address:
Optional Job description
Amino Acid Sequence
or upload contents of sequence file
or UniProt accession
Modelling Mode	Normal Intensive AlphaThread Traditional Phyre2 Test mode
Please tick as appropriate.	\|NOT for Profit\| FOR Profit (Commercial)\| Other\|

Examples of running Phyre2.2 on UniProt accession P0DV45 in Normal, Intensive and AlphaThread modes Info icon

6251822 submissions since Feb 14 2011

Other Resources
Missense3D Portal:	a suite of resources for the analysis of amino acid substitutions in proteins
GWYRE:	contains modeled and experimentally determined structures of human proteins and protein complexes, annotated by phenotypic effects of genetic mutations.

Phyre is now FREE for commercial users!

All images and data generated by Phyre2 are free to use in any publication with acknowledgement

Accessibility Statement

Please cite: Phyre2.2: A Community Resource for Template-based Protein Structure Prediction

Powell HR et al. Journal of Molecular Biology (2025) 437 168960 - 168969 DOI: https://doi.org/10.1016/j.jmb.2025.168960

© Structural Bioinformatics Group, Imperial College, London
Michael Sternberg
Disclaimer
Terms and Conditions

Phyre2 is part of Genome3D