PHYRE2 Protein Fold Recognition Server

Submit both a sequence and a structure. Phyre will attempt to align the sequence and structure and construct a model of your sequence

Submit a file containing up to 100 sequences for automated modelling by Phyre (to increase limit contact Harry Powell)

Use Phyre in reverse. Submit a PDB structure and search that structure against a wide range of genomes

Backphyre options
E-mail address
Optional job description
Upload PDB format file	Single chain only
Choose 1 or more genomes to search (ctrl-click or cmd-click) * private genomes are only searchable by associated users

Search your sequence against a wide range of genomes

Gensearch options
E-mail address
Optional job description
Amino Acid Sequence
Choose 1 or more genomes to search (ctrl-click or cmd-click)

Batch processing
E-mail address
Job description
Upload file of FASTA sequences (Max 100 seqs)		Traditional Phyre2

MSA options
E-mail address
Optional job description
Multiple sequence alignment
Or upload alignment
Alignment format
Alignment method	local global
Secondary structure scoring	yes no predicted vs predicted only
Secondary structure weight

Other Resources
Missense3D Portal:	a suite of resources for the analysis of amino acid substitutions in proteins
GWYRE:	contains modeled and experimentally determined structures of human proteins and protein complexes, annotated by phenotypic effects of genetic mutations.

One to one threading
E-mail address
Optional job description
Amino Acid Sequence
or upload contents of sequence file
or UniProt accession
Upload PDB format file	Single chain only
or AlphaFold Model	"best" model or UniProt ID confidence

Please cite: The Phyre2 web portal for protein modeling, prediction and analysis
Kelley LA et al. Nature Protocols 10, 845-858 (2015) [paper] [Citation link]

Expert Mode