Email l.a.kelley@imperial.ac.uk Description C6Y4B9 Date Sun Jul 8 11:45:08 BST 2012 Unique Job ID 358cbdc858c09f31

Summary

Top model

Image coloured by rainbow N → C terminus Close Jmol

Model (left) based on template d2nvpa1 Top template information Fold:alpha/alpha toroid Superfamily:Six-hairpin glycosidases Family:CPF0428-like Confidence and coverage Confidence: 27.9% Coverage: 16% 14 residues ( 16% of your sequence) have been modelled with 27.9% confidence by the single highest scoring template. You may wish to submit your sequence to Phyrealarm. This will automatically scan your sequence every week for new potential templates as they appear in the Phyre2 library. Please note: You must be registered and logged in to use Phyrealarm. 3D viewing Interactive 3D view in Jmol

Sequence analysis

Secondary structure and disorder prediction

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2nvp chain A domain 1 Region: 68 - 81 Aligned: 14 Modelled: 14 Confidence: 27.9% Identity: 43% Fold: alpha/alpha toroid Superfamily: Six-hairpin glycosidases Family: CPF0428-like Phyre2 PDB 4emo chain B Region: 64 - 74 Aligned: 11 Modelled: 11 Confidence: 26.8% Identity: 73% PDB header:protein binding Chain: B: PDB Molecule:sharpin; PDBTitle: crystal structure of the ph domain of sharpin Phyre2 PDB 2p0v chain A domain 1 Region: 70 - 81 Aligned: 12 Modelled: 12 Confidence: 25.1% Identity: 42% Fold: alpha/alpha toroid Superfamily: Six-hairpin glycosidases Family: CPF0428-like Phyre2 PDB 2p0v chain A Region: 70 - 81 Aligned: 12 Modelled: 12 Confidence: 25.1% Identity: 42% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical protein bt3781; PDBTitle: crystal structure of bt3781 protein from bacteroides2 thetaiotaomicron, northeast structural genomics target3 btr58 Phyre2 PDB 2j3w chain B domain 1 Region: 53 - 89 Aligned: 37 Modelled: 37 Confidence: 20.6% Identity: 19% Fold: Ligand-binding domain in the NO signalling and Golgi transport Superfamily: Ligand-binding domain in the NO signalling and Golgi transport Family: TRAPP components Phyre2 PDB 3qsp chain B Region: 68 - 81 Aligned: 14 Modelled: 14 Confidence: 16.9% Identity: 29% PDB header:hydrolase Chain: B: PDB Molecule:putative uncharacterized protein; PDBTitle: analysis of a new family of widely distributed metal-independent alpha2 mannosidases provides unique insight into the processing of n-linked3 glycans, streptococcus pneumoniae sp_2144 non-productive substrate4 complex with alpha-1,6-mannobiose Phyre2 PDB 1j0g chain A Region: 53 - 71 Aligned: 19 Modelled: 19 Confidence: 15.0% Identity: 47% Fold: beta-Grasp (ubiquitin-like) Superfamily: Ubiquitin-like Family: BM-002-like Phyre2 PDB 2loq chain A Region: 51 - 59 Aligned: 9 Modelled: 9 Confidence: 8.9% Identity: 56% PDB header:membrane protein Chain: A: PDB Molecule:interferon alpha-inducible protein 27-like protein 1; PDBTitle: backbone structure of human membrane protein fam14b (interferon alpha-2 inducible protein 27-like protein 1) Phyre2 PDB 2yzy chain A Region: 57 - 74 Aligned: 17 Modelled: 18 Confidence: 7.0% Identity: 59% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:putative uncharacterized protein ttha1012; PDBTitle: crystal structure of uncharacterized conserved protein from thermus2 thermophilus hb8 Phyre2 PDB 2j3t chain B Region: 53 - 73 Aligned: 21 Modelled: 20 Confidence: 6.2% Identity: 19% PDB header:transport Chain: B: PDB Molecule:trafficking protein particle complex subunit 6a; PDBTitle: the crystal structure of the bet3-trs33-bet5-trs23 complex. Phyre2 PDB 3i6p chain F Region: 55 - 65 Aligned: 11 Modelled: 11 Confidence: 6.1% Identity: 45% PDB header:structural protein Chain: F: PDB Molecule:ethanolamine utilization protein eutm; PDBTitle: ethanolamine utilization microcompartment shell subunit, eutm Phyre2 PDB 3cue chain H Region: 53 - 87 Aligned: 35 Modelled: 35 Confidence: 5.6% Identity: 20% PDB header:protein transport Chain: H: PDB Molecule:transport protein particle 31 kda subunit; PDBTitle: crystal structure of a trapp subassembly activating the rab ypt1p Phyre2 PDB 2cfh chain D Region: 53 - 73 Aligned: 21 Modelled: 21 Confidence: 5.6% Identity: 19% PDB header:transport Chain: D: PDB Molecule:trafficking protein particle complex subunit 6b; PDBTitle: structure of the bet3-tpc6b core of trapp Phyre2 PDB 3mjh chain D Region: 57 - 62 Aligned: 6 Modelled: 6 Confidence: 5.4% Identity: 83% PDB header:protein transport Chain: D: PDB Molecule:early endosome antigen 1; PDBTitle: crystal structure of human rab5a in complex with the c2h2 zinc finger2 of eea1 Phyre2 1 d2nvpa1 2 c4emoB_ 3 d2p0va1 4 c2p0vA_ 5 d2j3wb1 6 c3qspB_ 7 d1j0ga_ 8 c2loqA_ 9 c2yzyA_ 10 c2j3tB_ 11 c3i6pF_ 12 c3cueH_ 13 c2cfhD_ 14 c3mjhD_

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0