Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	N	E	Q	N	L	K	H	V	I	A	L	L	L	E	D	A	K	R	L	Q	Q	I	E	P	N	A	G	T	E	A	R	I	L	L	A	K	Q	A	L	K	T	C	G	A	Q	A	P	G	R	T	E	F	M	N	F	M	A	N	T
Secondary structure
SS confidence
Disorder		?	?																					?	?	?	?		?															?		?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.
Sequence		L	T	P	L	P	C	N	G	E	K	V	S	R	V	Y	H	D	T	M	V	K	A	L	R	I	E	L	D	G	L	R	R	K	I	V	M	N	E	I	V	T	N
Secondary structure
SS confidence
Disorder																																								?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1sf9 chain A Region: 1 - 51 Aligned: 51 Modelled: 51 Confidence: 73.9% Identity: 37% Fold: SH3-like barrel Superfamily: Hypothetical protein YfhH Family: Hypothetical protein YfhH Phyre2 PDB 2yxy chain A Region: 1 - 51 Aligned: 51 Modelled: 51 Confidence: 66.3% Identity: 31% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical conserved protein, gk0453; PDBTitle: crystarl structure of hypothetical conserved protein, gk0453 Phyre2 PDB 2kbz chain A Region: 2 - 18 Aligned: 17 Modelled: 17 Confidence: 29.1% Identity: 35% PDB header:viral protein Chain: A: PDB Molecule:15 protein (bacteriophage spp1 complete PDBTitle: nmr structure of protein gp15 of bacteriophage spp1 Phyre2 PDB 3h5j chain A Region: 25 - 76 Aligned: 52 Modelled: 52 Confidence: 20.3% Identity: 21% PDB header:lyase Chain: A: PDB Molecule:3-isopropylmalate dehydratase small subunit; PDBTitle: leud_1-168 small subunit of isopropylmalate isomerase (rv2987c) from2 mycobacterium tuberculosis Phyre2 PDB 1onv chain B Region: 77 - 90 Aligned: 14 Modelled: 14 Confidence: 15.7% Identity: 57% PDB header:transcription Chain: B: PDB Molecule:serine phosphatase fcp1a; PDBTitle: nmr structure of a complex containing the tfiif subunit2 rap74 and the rnap ii ctd phosphatase fcp1 Phyre2 PDB 2e74 chain D domain 2 Region: 49 - 58 Aligned: 10 Modelled: 10 Confidence: 12.8% Identity: 60% Fold: Single transmembrane helix Superfamily: ISP transmembrane anchor Family: ISP transmembrane anchor Phyre2 PDB 3q3w chain B Region: 25 - 76 Aligned: 52 Modelled: 52 Confidence: 10.7% Identity: 21% PDB header:transferase Chain: B: PDB Molecule:3-isopropylmalate dehydratase small subunit; PDBTitle: isopropylmalate isomerase small subunit from campylobacter jejuni. Phyre2 PDB 1qb2 chain A Region: 1 - 29 Aligned: 29 Modelled: 29 Confidence: 10.5% Identity: 24% Fold: Signal peptide-binding domain Superfamily: Signal peptide-binding domain Family: Signal peptide-binding domain Phyre2 PDB 1ifl chain A Region: 24 - 42 Aligned: 18 Modelled: 19 Confidence: 9.8% Identity: 50% PDB header:virus Chain: A: PDB Molecule:inovirus; PDBTitle: molecular models and structural comparisons of native and2 mutant class i filamentous bacteriophages ff (fd, f1, m13),3 if1 and ike Phyre2 PDB 2gbo chain B Region: 7 - 23 Aligned: 17 Modelled: 15 Confidence: 8.7% Identity: 41% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:upf0358 protein ef2458; PDBTitle: protein of unknown function ef2458 from enterococcus faecalis Phyre2 PDB 2gbo chain A domain 1 Region: 7 - 23 Aligned: 17 Modelled: 15 Confidence: 8.7% Identity: 41% Fold: Open three-helical up-and-down bundle Superfamily: EF2458-like Family: EF2458-like Phyre2 PDB 1t0w chain A Region: 41 - 51 Aligned: 11 Modelled: 11 Confidence: 7.9% Identity: 45% Fold: Knottins (small inhibitors, toxins, lectins) Superfamily: Plant lectins/antimicrobial peptides Family: Hevein-like agglutinin (lectin) domain Phyre2 PDB 4a1a chain H Region: 22 - 46 Aligned: 25 Modelled: 25 Confidence: 6.9% Identity: 32% PDB header:ribosome Chain: H: PDB Molecule:60s ribosomal protein l10; PDBTitle: t.thermophila 60s ribosomal subunit in complex with2 initiation factor 6. this file contains 5s rrna,3 5.8s rrna and proteins of molecule 3. Phyre2 PDB 3t97 chain A Region: 67 - 79 Aligned: 13 Modelled: 13 Confidence: 6.6% Identity: 31% PDB header:protein transport Chain: A: PDB Molecule:nuclear pore glycoprotein p62; PDBTitle: molecular architecture of the transport channel of the nuclear pore2 complex: nup62/nup54 Phyre2 PDB 1szh chain A Region: 51 - 89 Aligned: 34 Modelled: 39 Confidence: 5.7% Identity: 29% Fold: Her-1 Superfamily: Her-1 Family: Her-1 Phyre2 PDB 2dw3 chain A Region: 23 - 44 Aligned: 22 Modelled: 22 Confidence: 5.3% Identity: 32% PDB header:photosynthesis Chain: A: PDB Molecule:intrinsic membrane protein pufx; PDBTitle: solution structure of the rhodobacter sphaeroides pufx2 membrane protein Phyre2 PDB 1gc5 chain A Region: 1 - 39 Aligned: 39 Modelled: 39 Confidence: 5.2% Identity: 13% Fold: Ribokinase-like Superfamily: Ribokinase-like Family: ADP-specific Phosphofructokinase/Glucokinase Phyre2 PDB 2pa2 chain A domain 1 Region: 22 - 47 Aligned: 26 Modelled: 26 Confidence: 5.2% Identity: 35% Fold: alpha/beta-Hammerhead Superfamily: Ribosomal protein L16p/L10e Family: Ribosomal protein L10e Phyre2 PDB 1oft chain C Region: 16 - 42 Aligned: 26 Modelled: 27 Confidence: 5.1% Identity: 19% PDB header:bacterial cell division inhibitor Chain: C: PDB Molecule:hypothetical protein pa3008; PDBTitle: crystal structure of sula from pseudomonas aeruginosa Phyre2 PDB 1ofu chain X Region: 16 - 42 Aligned: 26 Modelled: 27 Confidence: 5.0% Identity: 19% Fold: P-loop containing nucleoside triphosphate hydrolases Superfamily: P-loop containing nucleoside triphosphate hydrolases Family: Bacterial cell division inhibitor SulA Phyre2 1 d1sf9a_ 2 c2yxyA_ 3 c2kbzA_ 4 c3h5jA_ 5 c1onvB_ 6 d2e74d2 7 c3q3wB_ 8 d1qb2a_ 9 c1iflA_ 10 c2gboB_ 11 d2gboa1 12 d1t0wa_ 13 c4a1aH_ 14 c3t97A_ 15 d1szha_ 16 c2dw3A_ 17 d1gc5a_ 18 d2pa2a1 19 c1oftC_ 20 d1ofux_

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1sf9a_ 73.9 37 Fold:SH3-like barrel Superfamily:Hypothetical protein YfhH Family:Hypothetical protein YfhH 2 c2yxyA_ 66.3 31 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical conserved protein, gk0453; PDBTitle: crystarl structure of hypothetical conserved protein, gk0453 3 c2kbzA_ 29.1 35 PDB header:viral protein Chain: A: PDB Molecule:15 protein (bacteriophage spp1 complete PDBTitle: nmr structure of protein gp15 of bacteriophage spp1 4 c3h5jA_ 20.3 21 PDB header:lyase Chain: A: PDB Molecule:3-isopropylmalate dehydratase small subunit; PDBTitle: leud_1-168 small subunit of isopropylmalate isomerase (rv2987c) from2 mycobacterium tuberculosis 5 c1onvB_ 15.7 57 PDB header:transcription Chain: B: PDB Molecule:serine phosphatase fcp1a; PDBTitle: nmr structure of a complex containing the tfiif subunit2 rap74 and the rnap ii ctd phosphatase fcp1 6 d2e74d2 12.8 60 Fold:Single transmembrane helix Superfamily:ISP transmembrane anchor Family:ISP transmembrane anchor 7 c3q3wB_ 10.7 21 PDB header:transferase Chain: B: PDB Molecule:3-isopropylmalate dehydratase small subunit; PDBTitle: isopropylmalate isomerase small subunit from campylobacter jejuni. 8 d1qb2a_ 10.5 24 Fold:Signal peptide-binding domain Superfamily:Signal peptide-binding domain Family:Signal peptide-binding domain 9 c1iflA_ 9.8 50 PDB header:virus Chain: A: PDB Molecule:inovirus; PDBTitle: molecular models and structural comparisons of native and2 mutant class i filamentous bacteriophages ff (fd, f1, m13),3 if1 and ike 10 c2gboB_ 8.7 41 PDB header:structural genomics, unknown function Chain: B: PDB Molecule:upf0358 protein ef2458; PDBTitle: protein of unknown function ef2458 from enterococcus faecalis 11 d2gboa1 8.7 41 Fold:Open three-helical up-and-down bundle Superfamily:EF2458-like Family:EF2458-like 12 d1t0wa_ 7.9 45 Fold:Knottins (small inhibitors, toxins, lectins) Superfamily:Plant lectins/antimicrobial peptides Family:Hevein-like agglutinin (lectin) domain 13 c4a1aH_ 6.9 32 PDB header:ribosome Chain: H: PDB Molecule:60s ribosomal protein l10; PDBTitle: t.thermophila 60s ribosomal subunit in complex with2 initiation factor 6. this file contains 5s rrna,3 5.8s rrna and proteins of molecule 3. 14 c3t97A_ 6.6 31 PDB header:protein transport Chain: A: PDB Molecule:nuclear pore glycoprotein p62; PDBTitle: molecular architecture of the transport channel of the nuclear pore2 complex: nup62/nup54 15 d1szha_ 5.7 29 Fold:Her-1 Superfamily:Her-1 Family:Her-1 16 c2dw3A_ 5.3 32 PDB header:photosynthesis Chain: A: PDB Molecule:intrinsic membrane protein pufx; PDBTitle: solution structure of the rhodobacter sphaeroides pufx2 membrane protein 17 d1gc5a_ 5.2 13 Fold:Ribokinase-like Superfamily:Ribokinase-like Family:ADP-specific Phosphofructokinase/Glucokinase 18 d2pa2a1 5.2 35 Fold:alpha/beta-Hammerhead Superfamily:Ribosomal protein L16p/L10e Family:Ribosomal protein L10e 19 c1oftC_ 5.1 19 PDB header:bacterial cell division inhibitor Chain: C: PDB Molecule:hypothetical protein pa3008; PDBTitle: crystal structure of sula from pseudomonas aeruginosa 20 d1ofux_ 5.0 19 Fold:P-loop containing nucleoside triphosphate hydrolases Superfamily:P-loop containing nucleoside triphosphate hydrolases Family:Bacterial cell division inhibitor SulA

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0