Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	K	M	L	R	D	P	L	F	W	L	I	A	L	F	V	A	L	I	F	W	L	P	Y	S	Q	P	L	F	A	A	L	F	P	Q	L	P	R	P	V	Y	Q	Q	E	S	F	A	A	L	A	L	A	H	F	W	L	V	G	I	S
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?																			?	?	?	?	?	?	?		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		S	L	F	A	V	I	I	G	T	G	A	G	I	A	V	T	R	P	W	G	A	E	F	R	P	L	V	E	T	I	A	A	V	G	Q	T	F	P	P	V	A	V	L	A	I	A	V	P	V	I	G	F	G	L	Q	P	A	I	I	A
Secondary structure
SS confidence
Disorder																			?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		L	I	L	Y	G	V	L	P	V	L	Q	A	T	L	A	G	L	G	A	I	D	A	S	V	T	E	V	A	K	G	M	G	M	S	R	G	Q	R	V	R	K	V	E	L	P	L	A	A	P	V	I	L	A	G	V	R	T	S	V	I
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		I	N	I	G	T	A	T	I	A	S	T	V	G	A	S	T	L	G	T	P	I	I	I	G	L	S	G	F	N	T	A	Y	V	I	Q	G	A	L	L	V	A	L	A	A	I	I	A	D	R	L	F	E	R	L	V	Q	A	L	S	Q
Secondary structure
SS confidence
Disorder																													?	?																													?		?
Disorder confidence
		.	.	.
Sequence		H	A	K
Secondary structure
SS confidence
Disorder		?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2onk chain C domain 1 Region: 1 - 234 Aligned: 233 Modelled: 234 Confidence: 100.0% Identity: 20% Fold: MetI-like Superfamily: MetI-like Family: MetI-like Phyre2 PDB 2onk chain C Region: 1 - 234 Aligned: 233 Modelled: 234 Confidence: 100.0% Identity: 20% PDB header:membrane protein Chain: C: PDB Molecule:molybdate/tungstate abc transporter, permease PDBTitle: abc transporter modbc in complex with its binding protein2 moda Phyre2 PDB 3d31 chain C domain 1 Region: 7 - 232 Aligned: 225 Modelled: 226 Confidence: 100.0% Identity: 18% Fold: MetI-like Superfamily: MetI-like Family: MetI-like Phyre2 PDB 3d31 chain D Region: 7 - 232 Aligned: 225 Modelled: 226 Confidence: 100.0% Identity: 18% PDB header:transport protein Chain: D: PDB Molecule:sulfate/molybdate abc transporter, permease PDBTitle: modbc from methanosarcina acetivorans Phyre2 PDB 3fh6 chain F Region: 7 - 243 Aligned: 237 Modelled: 237 Confidence: 100.0% Identity: 16% PDB header:transport protein Chain: F: PDB Molecule:maltose transport system permease protein malf; PDBTitle: crystal structure of the resting state maltose transporter from e.2 coli Phyre2 PDB 2r6g chain F Region: 34 - 230 Aligned: 196 Modelled: 197 Confidence: 100.0% Identity: 18% PDB header:hydrolase/transport protein Chain: F: PDB Molecule:maltose transport system permease protein malf; PDBTitle: the crystal structure of the e. coli maltose transporter Phyre2 PDB 2r6g chain F domain 2 Region: 34 - 234 Aligned: 200 Modelled: 201 Confidence: 100.0% Identity: 16% Fold: MetI-like Superfamily: MetI-like Family: MetI-like Phyre2 PDB 3dhw chain A domain 1 Region: 46 - 230 Aligned: 185 Modelled: 185 Confidence: 99.9% Identity: 24% Fold: MetI-like Superfamily: MetI-like Family: MetI-like Phyre2 PDB 2r6g chain G domain 1 Region: 2 - 233 Aligned: 231 Modelled: 232 Confidence: 99.9% Identity: 21% Fold: MetI-like Superfamily: MetI-like Family: MetI-like Phyre2 PDB 1ntc chain A Region: 131 - 162 Aligned: 32 Modelled: 32 Confidence: 26.5% Identity: 16% Fold: DNA/RNA-binding 3-helical bundle Superfamily: Homeodomain-like Family: FIS-like Phyre2 PDB 1umq chain A Region: 131 - 162 Aligned: 32 Modelled: 32 Confidence: 25.1% Identity: 22% PDB header:dna-binding protein Chain: A: PDB Molecule:photosynthetic apparatus regulatory protein; PDBTitle: solution structure and dna binding of the effector domain2 from the global regulator prra(rega) from r. sphaeroides:3 insights into dna binding specificity Phyre2 PDB 1umq chain A Region: 131 - 162 Aligned: 32 Modelled: 32 Confidence: 25.1% Identity: 22% Fold: DNA/RNA-binding 3-helical bundle Superfamily: Homeodomain-like Family: FIS-like Phyre2 PDB 3rko chain F Region: 2 - 82 Aligned: 81 Modelled: 81 Confidence: 16.8% Identity: 10% PDB header:oxidoreductase Chain: F: PDB Molecule:nadh-quinone oxidoreductase subunit j; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 e. coli at 3.0 angstrom resolution Phyre2 PDB 1fip chain A Region: 131 - 162 Aligned: 32 Modelled: 32 Confidence: 14.9% Identity: 22% Fold: DNA/RNA-binding 3-helical bundle Superfamily: Homeodomain-like Family: FIS-like Phyre2 PDB 1eto chain B Region: 131 - 162 Aligned: 32 Modelled: 32 Confidence: 13.7% Identity: 22% Fold: DNA/RNA-binding 3-helical bundle Superfamily: Homeodomain-like Family: FIS-like Phyre2 PDB 3e7l chain D Region: 131 - 162 Aligned: 32 Modelled: 32 Confidence: 12.3% Identity: 19% PDB header:transcription regulator Chain: D: PDB Molecule:transcriptional regulator (ntrc family); PDBTitle: crystal structure of sigma54 activator ntrc4's dna binding2 domain Phyre2 PDB 1etx chain A Region: 131 - 162 Aligned: 32 Modelled: 32 Confidence: 11.7% Identity: 22% Fold: DNA/RNA-binding 3-helical bundle Superfamily: Homeodomain-like Family: FIS-like Phyre2 PDB 2jwa chain A Region: 51 - 84 Aligned: 34 Modelled: 34 Confidence: 11.6% Identity: 21% PDB header:transferase Chain: A: PDB Molecule:receptor tyrosine-protein kinase erbb-2; PDBTitle: erbb2 transmembrane segment dimer spatial structure Phyre2 PDB 2cw1 chain A Region: 141 - 155 Aligned: 15 Modelled: 15 Confidence: 10.3% Identity: 27% PDB header:de novo protein Chain: A: PDB Molecule:sn4m; PDBTitle: solution structure of the de novo-designed lambda cro fold2 protein Phyre2 PDB 2d7d chain B Region: 134 - 164 Aligned: 31 Modelled: 31 Confidence: 7.7% Identity: 19% PDB header:hydrolase/dna Chain: B: PDB Molecule:40-mer from uvrabc system protein b; PDBTitle: structural insights into the cryptic dna dependent atp-ase2 activity of uvrb Phyre2 1 d2onkc1 2 c2onkC_ 3 d3d31c1 4 c3d31D_ 5 c3fh6F_ 6 c2r6gF_ 7 d2r6gf2 8 d3dhwa1 9 d2r6gg1 10 d1ntca_ 11 c1umqA_ 12 d1umqa_ 13 c3rkoF_ 14 d1fipa_ 15 d1etob_ 16 c3e7lD_ 17 d1etxa_ 18 c2jwaA_ 19 c2cw1A_ 20 c2d7dB_

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d2onkc1 100.0 20 Fold:MetI-like Superfamily:MetI-like Family:MetI-like 2 c2onkC_ 100.0 20 PDB header:membrane protein Chain: C: PDB Molecule:molybdate/tungstate abc transporter, permease PDBTitle: abc transporter modbc in complex with its binding protein2 moda 3 d3d31c1 100.0 18 Fold:MetI-like Superfamily:MetI-like Family:MetI-like 4 c3d31D_ 100.0 18 PDB header:transport protein Chain: D: PDB Molecule:sulfate/molybdate abc transporter, permease PDBTitle: modbc from methanosarcina acetivorans 5 c3fh6F_ 100.0 16 PDB header:transport protein Chain: F: PDB Molecule:maltose transport system permease protein malf; PDBTitle: crystal structure of the resting state maltose transporter from e.2 coli 6 c2r6gF_ 100.0 18 PDB header:hydrolase/transport protein Chain: F: PDB Molecule:maltose transport system permease protein malf; PDBTitle: the crystal structure of the e. coli maltose transporter 7 d2r6gf2 100.0 16 Fold:MetI-like Superfamily:MetI-like Family:MetI-like 8 d3dhwa1 99.9 24 Fold:MetI-like Superfamily:MetI-like Family:MetI-like 9 d2r6gg1 99.9 21 Fold:MetI-like Superfamily:MetI-like Family:MetI-like 10 d1ntca_ 26.5 16 Fold:DNA/RNA-binding 3-helical bundle Superfamily:Homeodomain-like Family:FIS-like 11 c1umqA_ 25.1 22 PDB header:dna-binding protein Chain: A: PDB Molecule:photosynthetic apparatus regulatory protein; PDBTitle: solution structure and dna binding of the effector domain2 from the global regulator prra(rega) from r. sphaeroides:3 insights into dna binding specificity 12 d1umqa_ 25.1 22 Fold:DNA/RNA-binding 3-helical bundle Superfamily:Homeodomain-like Family:FIS-like 13 c3rkoF_ 16.8 10 PDB header:oxidoreductase Chain: F: PDB Molecule:nadh-quinone oxidoreductase subunit j; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 e. coli at 3.0 angstrom resolution 14 d1fipa_ 14.9 22 Fold:DNA/RNA-binding 3-helical bundle Superfamily:Homeodomain-like Family:FIS-like 15 d1etob_ 13.7 22 Fold:DNA/RNA-binding 3-helical bundle Superfamily:Homeodomain-like Family:FIS-like 16 c3e7lD_ 12.3 19 PDB header:transcription regulator Chain: D: PDB Molecule:transcriptional regulator (ntrc family); PDBTitle: crystal structure of sigma54 activator ntrc4's dna binding2 domain 17 d1etxa_ 11.7 22 Fold:DNA/RNA-binding 3-helical bundle Superfamily:Homeodomain-like Family:FIS-like 18 c2jwaA_ 11.6 21 PDB header:transferase Chain: A: PDB Molecule:receptor tyrosine-protein kinase erbb-2; PDBTitle: erbb2 transmembrane segment dimer spatial structure 19 c2cw1A_ 10.3 27 PDB header:de novo protein Chain: A: PDB Molecule:sn4m; PDBTitle: solution structure of the de novo-designed lambda cro fold2 protein 20 c2d7dB_ 7.7 19 PDB header:hydrolase/dna Chain: B: PDB Molecule:40-mer from uvrabc system protein b; PDBTitle: structural insights into the cryptic dna dependent atp-ase2 activity of uvrb

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0