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Job DescriptionP06864
Confidence100.00%DateThu Jan 5 10:59:27 GMT 2012
Rank1Aligned Residues977
% Identity33%Templatec3mv14_
PDB info PDB header:hydrolaseChain: 4: PDB Molecule:beta-galactosidase; PDBTitle: e.coli (lacz) beta-galactosidase (r599a) in complex with guanidinium
Resolution2.20 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   2.......10.........20.........30.........40.........50.........60.........70.........80.
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Query Sequence  NRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPLSGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQ
Query Conservation    



 
   

    
   
      
       
    



 
 
            
       
  
 

  
 
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Template Conservation    


  
   

               
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Template Sequence  RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRP. . SQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQ
Template Known Secondary structure 
GGG
TT
SS




S



BSST

..
TT

SSGGGS
TS

TT
S

TT
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   14.....20.........30.........40....... ..50.........60.........70.........80.........90.
 
   82.......90.........100.........110.........120... ......130.........140.........150.........160
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Query Sequence  MEGHGKLQYTDEGFPFPIDVPFVPSDNPTGAYQRIFTLSDGW. QGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
Query Conservation    
   
 
 
   
     
 
   
  
 


 
 

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Template Conservation    
     
 
  

     
 
   
  
 


 
 

  
  



 
 



   
 




  

   
   
 

Template Sequence  MHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
Template Known Secondary structure  TTTSS

SSS
SS
S
TT


SS



SSSS

BSTT
TTS
Template Predicted Secondary structure 









































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   92.......100.........110.........120.........130.........140.........150.........160.........170.
 
   161........170.........180.........190.........200.........210.........220.........230.........240
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Query Sequence  DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRTDFDEAYCDATLSCEVVLENLAA
Query Conservation 


  

 


 
 
 
    
 
  
 

 
  


 
 
 
   
   
 
  
 
 
      
 
 
 
 
 
   
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Template Conservation 


  
  
 
 


 
    
 
  
 

 
  


 


 
   
   
 
  
 
 
      
 
 
 
 
     
Template Sequence  DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCGELR
Template Known Secondary structure 
TTT

SSS

GGGGGB

S

B

S

SS
TTSSS


Template Predicted Secondary structure 




































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   172.......180.........190.........200.........210.........220.........230.........240.........250.
 
   241........250.........260 .........270.........280.........290.........300.........310......
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Query Sequence  SPVVTTLEYTLFDGERVVHS. . . . SAIDHLAIEKLTSASFAFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVG
Query Conservation        
   
 
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Template Conservation      
   
                                  
  
 


 
 
 

 
   
    
   
      
Template Sequence  DYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVG
Template Known Secondary structure  TTTTS


TT
TTS

BB
SSSB

TT


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   252.......260.........270.........280.........290.........300.........310.........320.........330.
 
   317..320.........330.........340.........350.........360.........370.........380.........390......
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Query Sequence  FRDIKVRDGLFWINNRYVMLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGLFVMA
Query Conservation 

 

     
 



 
 




 
   
  
     
    

 


  
 
 

 



    






 

 
 
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Template Conservation 

 


    
 


 

 




 
   
  
     
    

 


  
 
 

 



    






 

 
  
Template Sequence  FREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVD
Template Known Secondary structure  B

TTTT





BTTTBT


TT


TTS


T
Template Predicted Secondary structure 




























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   332.......340.........350.........360.........370.........380.........390.........400.........410.
 
   397..400.........410.........420.........430.........440.........450.........460.........470......
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Query Sequence  ETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNESGYGCNIRAMYHAAKALDDTRLVHYEE
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Template Conservation 

  
 

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Template Sequence  EANIETHGMVP. . . MNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEG
Template Known Secondary structure 
S
B
TTSSS...TTTTTT
GGGTT
TT

SS




TTS


T
Template Predicted Secondary structure 









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   412.......420.. .......430.........440.........450.........460.........470.........480........
 
   477..480.........490.........500. ........510.........520.........530.........540...
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Query Sequence  . DRDAEVVDIISTMYTRVPLMNEFGE. . . . . . . . . . . . YPHPKPRIICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVW
Query Conservation  .       

 
  
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Template Conservation          

 
 

                           

 
  

 




  
 
  

          




Template Sequence  GGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW
Template Known Secondary structure  TTSSSTT
SSB

BS

SS

B

SSS
B

TSTT



S


SS

TT
TTS
Template Predicted Secondary structure 




































Template SS confidence 















































































   489490.........500.........510.........520.........530.........540.........550.........560........
 
   544.....550.........560.........570.........580.........590.........600.........610.........620...
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Query Sequence  EWCDHGIQAQDDHGNVWYKFGGDYGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHARDLTRGELKVENKLWFT
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Template Conservation     
         
     






 
 
  
   


  

 


   
 
    

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Template Sequence  DWVDQSLIKYDENGNPWSAYGGDFGDTPNDAQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSG. . . QTIEVTSEYLFR
Template Known Secondary structure 
SB



TT


TTTTS

S

GGGG


SB
TTS
B
TT
ST...T

SS
Template Predicted Secondary structure 






























...




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   569570.........580.........590.........600.........610.........620.........630... ......640.....
 
   624.....630.........640.........650.........660.. .......670.........680.........690.........700.
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Query Sequence  TLDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQIT. . LPQLDAREAFLNITVTKDSRTRYSEAGHPIATYQFPLKE
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Template Conservation   
      
 
  

  
  
   
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Template Sequence  HSDNELLHWMVALDGKPLASGEVPL. DVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAE
Template Known Secondary structure 

TT
TT
.


TT







SS

S

SS

TT
Template Predicted Secondary structure 







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   646...650.........660.........670 .........680.........690.........700.........710.........720....
 
   702.......710.........720.........730.........740.........750.........760.........770......
Predicted Secondary structure 












































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Query Sequence  NTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYE. . . . .
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Template Conservation               ..           
        
    
 
      
  

        


 



          
Template Sequence  NLSVTLPAASHAI. . PHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRID
Template Known Secondary structure 








S

..

SSTTTTT
STT






TT

SSS

Template Predicted Secondary structure 












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Template SS confidence 















































































   725....730....... ..740.........750.........760.........770.........780.........790.........800..
 
   777..780.........790.........800.........810.........820.........830.........840.........850.
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Query Sequence  . . . . . GLWQPNHLQIMQEHLRDFAVEQSDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIP
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Template Conservation         
  

                   
 
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Template Sequence  PNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQG. KTLFISRKTYRIDGSGQMAITVDVEVASDTPH. PA
Template Known Secondary structure  TTSTTTT
TTTT.TTS

TTS

.
S
Template Predicted Secondary structure 












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   803......810.........820.........830.........840...... ...850.........860.........870........ .880
 
   852.......860.........870.........880.........890.........900.........910.........920.........930.
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Query Sequence  CIGFTMGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTALTNRHGNGLLVVPQR
Query Conservation 
 
    

       
 



 
 
 

      
 
   
      
  


 


 



  
    
 

 
    
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Template Conservation 
 
    
      
 
 
 

 
 
 

      
 
   
      
  


 


 



  
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Template Sequence  RIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRG. . . . . . .
Template Known Secondary structure  S


SSS

BTTB
SS
GGGGS


SS

S


TT.......
Template Predicted Secondary structure 



































.......
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   881........890.........900.........910.........920.........930.........940.........950...
 
   932.......940.........950.........960.........970... ......980.........990........
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Query Sequence  PINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGS. NSWGSEVLDSWRVWFRDFSYGFTLL
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Template Conservation     

   

   
  
 
  

       
 

  
 



  
 
      
          
 
 
Template Sequence  DFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWC
Template Known Secondary structure  SS

SSGGG



SS




S
SSS



GGGS

SS
Template Predicted Secondary structure 
































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   954.....960.........970.........980.........990.........1000.........1010.........1020.
 
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Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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