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Job DescriptionP06864
Confidence100.00%DateThu Jan 5 10:59:27 GMT 2012
Rank2Aligned Residues975
% Identity34%Templatec1jz6C_
PDB info PDB header:hydrolaseChain: C: PDB Molecule:beta-galactosidase; PDBTitle: e. coli (lacz) beta-galactosidase in complex with galacto-2 tetrazole
Resolution2.10 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   2.......10.........20.........30.........40.........50.........60.........70.........80.
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Query Sequence  NRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPLSGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQ
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Template Sequence  RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRP. . SQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQ
Template Known Secondary structure 
GGG
TT
SS




S



BSST

..
TT

SSGGG

TS

TT
S

TT
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   14.....20.........30.........40....... ..50.........60.........70.........80.........90.
 
   82.......90.........100.........110.........120... ......130.........140.........150.........160
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Query Sequence  MEGHGKLQYTDEGFPFPIDVPFVPSDNPTGAYQRIFTLSDGW. QGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
Query Conservation    
   
 
 
   
     
 
   
  
 


 
 

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Template Conservation    
   
 
 
  

     
 
   
  
 


 
 

  
  



 
 



   
 




  

   
   
 

Template Sequence  MHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
Template Known Secondary structure  TTTSS

SSS
SS


TT


SS


SSS
STT
TTS
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   92.......100.........110.........120.........130.........140.........150.........160.........170.
 
   161........170.........180.........190.........200.........210.........220.........230.........240
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Query Sequence  DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRTDFDEAYCDATLSCEVVLENLAA
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Template Conservation 


  
  


 


 
   

 
  
 

 
  


 


 
   
   
 
  
 
 
      
 
 
 
       
Template Sequence  DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCGELR
Template Known Secondary structure 
TTT

SS

GGGGGB

S


S

SS
TTSSS


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   172.......180.........190.........200.........210.........220.........230.........240.........250.
 
   241........250.........260.... .....270.........280.........290.........300.........310....
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Query Sequence  SPVVTTLEYTLFDGERVVHSSAID. . . . . . HLAIEKLTSASFAFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQR
Query Conservation        
   
 
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Template Sequence  DYLR. . VTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD
Template Known Secondary structure  TT..TT
S




BTTB

TTS

BB
SSSB

TTS
Template Predicted Secondary structure 

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   252... ....260.........270.........280.........290.........300.........310.........320.........
 
   315....320.........330.........340.........350.........360.........370.........380.........390....
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Query Sequence  VGFRDIKVRDGLFWINNRYVMLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGLFV
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Template Sequence  VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYV
Template Known Secondary structure 
B

TTTT





BTTTBT


TT


TTS


T
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   330.........340.........350.........360.........370.........380.........390.........400.........
 
   395....400.........410.........420.........430.........440.........450.........460.........470....
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Query Sequence  MAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNESGYGCNIRAMYHAAKALDDTRLVHY
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Template Sequence  VDEANIETHGMVP. . . MNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQY
Template Known Secondary structure 
S
B
TTSSS...TTTTTT
GGGTT
TT

BS




TTS

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   410.........420.. .......430.........440.........450.........460.........470.........480......
 
   475. ...480.........490.........500. ........510.........520.........530.........540.
Predicted Secondary structure 

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Query Sequence  EE. DRDAEVVDIISTMYTRVPLMNEFGE. . . . . . . . . . . . YPHPKPRIICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHY
Query Conservation 
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Template Conservation 
         

 
 

                           

 
  

 




  
 
  

          


Template Sequence  EGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGF
Template Known Secondary structure 
TTTS
STT
SSB

BS

SS

B

SSS
B

TTSTT



S


SS

TT
TT
Template Predicted Secondary structure 












































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   487..490.........500.........510.........520.........530.........540.........550.........560......
 
   542.......550.........560.........570.........580.........590.........600.........610.........620.
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Query Sequence  VWEWCDHGIQAQDDHGNVWYKFGGDYGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHARDLTRGELKVENKLW
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Template Conservation 

   
         
     






 
 
  
   


  

 


   
 
    

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Template Sequence  VWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSG. . . QTIEVTSEYL
Template Known Secondary structure  S
SB



TT


TTTTS
SS

GGGG


SB
TTS
B
TT
ST...T

S
Template Predicted Secondary structure 































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   567..570.........580.........590.........600.........610.........620.........630... ......640...
 
   622.......630.........640.........650.........660.. .......670.........680.........690.........
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Query Sequence  FTTLDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQIT. . LPQLDAREAFLNITVTKDSRTRYSEAGHPIATYQFPL
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Template Conservation 
  
      
 
  

  
  
   
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Template Sequence  FRHSDNELLHWMVALDGKPLASGEVPL. DVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRL
Template Known Secondary structure  S


TT
TT
.


TT







SSS
S

SS

TT
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   644.....650.........660.........670 .........680.........690.........700.........710.........720..
 
   700.........710.........720.........730.........740.........750.........760.........770.....
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Query Sequence  KENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEY. . . .
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Template Sequence  AENLSVTLPAASHAI. . PHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATR
Template Known Secondary structure 








S

..

SSTTTTTSSTT






TT
BTTB
Template Predicted Secondary structure 














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   723......730....... ..740.........750.........760.........770.........780.........790.........800
 
   776...780.........790.........800.........810.........820.........830.........840.........
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Query Sequence  . . . . . . EGLWQPNHLQIMQEHLRDFAVEQSDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHI
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Template Sequence  IDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQG. KTLFISRKTYRIDGSGQMAITVDVEVASDTPH.
Template Known Secondary structure 

TTSTTTT

SSTT.TTS

TTS

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   801........810.........820.........830.........840...... ...850.........860.........870........
 
   850.........860.........870.........880.........890.........900.........910.........920.........
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Query Sequence  IPCIGFTMGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTALTNRHGNGLLVVP
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Template Sequence  PARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRG. . . . .
Template Known Secondary structure 
SS


SSS

BTTB
SS
GGGG



SS

S


TT.....
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   879880.........890.........900.........910.........920.........930.........940.........950...
 
   930.........940.........950.........960.........970... ......980.........990........
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Query Sequence  QRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGS. NSWGSEVLDSWRVWFRDFSYGFTLL
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Template Conservation  ..   


  

   
  
 
  

       
 

    



  


      
          
   
Template Sequence  . . DFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWC
Template Known Secondary structure  ..S


S
GGG



SS




S
SSS



GGGS

SS
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   954.....960.........970.........980.........990.........1000.........1010.........1020.
 
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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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