Return to main results Retrieve Phyre Job Id

Job DescriptionP0A8V2
Confidence100.00%DateWed Jan 25 15:20:19 GMT 2012
Rank6Aligned Residues892
% Identity30%Templated1twfb_
SCOP infobeta and beta-prime subunits of DNA dependent RNA-polymerase beta and beta-prime subunits of DNA dependent RNA-polymerase RNA-polymerase beta
Resolution2.30

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   10.........20.........30.........40.........50.........60.........70........ .
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Query Sequence  RIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPEGQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEP. . . . . . . . . . V
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Template Sequence  TAEDSWAVISAFFREKGLVSQQLDSFNQFVDY. . . . . . TLQDIICEDSTL. . . . . . IEISFGKIYVTKPMVNESDGVTHA
Template Known Secondary structure 
TTTT
T......TT
B
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SSS

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   26...30.........40.........50....... ..60......... 70.........80.........90...
 
   80.........90.........100.........110.........120.........130... ..
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Query Sequence  FDVQECQIRGVTYSAPLRVKLRLVIYEREAPEGTVKDIKEQEVYMGEIPLMTDN. . . . . . . . . . . . . . . . . . . . . . . . GT
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Template Sequence  LYPQEARLRNLTYSSGLFVDVKKRTYE. . . . . . . . . . . . . . KVFIGRLPIMLRSKNCYLSEATESDLYKLKECPFDMGGY
Template Known Secondary structure 

TT









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TTSTTSGGGT

TT

TT



S
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   112.......120.........130........ .140.........150.........160.........170.......
 
   136...140.........150.........160.........170.........180.........190.........200 ...
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Query Sequence  FVINGTERVIVSQLHRSPGVFFDSDKGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDR. . . . . . . . . . . . RRK
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Template Sequence  FIINGSEKVLIAQERSAGNIVQVFKKAAP. . . SPISHVAEIRSALEKGSRFISTLQVKLYGREGSSARTIKATLPYIKQD
Template Known Secondary structure  TT

SS



TT...SS


SSS




S
SS
SS


TT
SS
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   203......210.........220.........230. ........240.........250.........260.........270.........
 
   204.....210........ .220.........230.........240.........250.........260.........270.........280..
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Query Sequence  LPATIILRALNYTTE. QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQLEKDDV
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Template Sequence  IPIVIIFRALGIIPDGEILEHIC. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Template Known Secondary structure  TT

S
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   280.........290.........300..
 
   283......290.........300.........310.........320.........330.........340.........350.........360..
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Query Sequence  KLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIETLFTNDLDHGPYISETLRVDPTNDRLSA
Query Conservation                                                                  
   
      
   
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Template Conservation  ...........................    
      
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Template Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . YDVNDWQMLEMLKPCVEDGF. . . . . . . . . . . . . . . . . . . . . . . . . VIQDRETA
Template Known Secondary structure  ...........................

TT
TT.........................T

S
Template Predicted Secondary structure  ...........................






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   303......310.........320.. .......330
 
   363......370.........380.........390.........400.........410..... ....420.........430.........440
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Query Sequence  LVEIYRMMRPGEPPTREAAESLFENLFFSEDRYDLSAVGRMKFNRSLLREEIE. . GSGILSKDDIIDVMKKLIDIRNGKG
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Template Sequence  LDFIGRRGTALGIKKEKRIQ. . . . . . . . . . . . . . . . . YAKDILQKEFLPHITQLEGFESRKAFFLGYMINRLLLCALDRK
Template Known Secondary structure  TTS
TTS
.................TSSTTT

SSS
TTSS
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   331........340.........350 .........360.........370.........380.........390...
 
   441........450.........460.........470.........480.........490.........500.........510. .....
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Query Sequence  EVDDIDHLGNRRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGSSQL. . . . SQFMD
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Template Sequence  DQDDRDHFGKKRLDLAGPLLAQLFKTLFKKLTKDIFRYMQR. . . . . . TVELAINAKTITSGLKYALATGNWGAGVSQVLN
Template Known Secondary structure 



TT
GGG
S......






T








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   394.....400.........410.........420.........430.... .....440.........450.........460.......
 
   517..520.........530.........540.........550.........560.........570.........580.
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Query Sequence  QNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYAQT. . . . . . . . . . . . . . .
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Template Sequence  RYTYSSTLSHLRRTNT. . . . . . . . . . PIAKPRQLHNTHWGLVCPAETPEGQACGLVKNLSLMSCISVGTDPMPIITFLSE
Template Known Secondary structure 
SST
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TTS

GGGTTTB
SS


SGGGBTTB
TT






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   485....490.........500 .........510.........520.........530.........540.........550....
 
   582.......590....
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Query Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . NEYGFLETPYRKV
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Template Sequence  WGMEPLEDYVPHQSPDATRVFVNGVWHGVHRNPARLMETLRTLRRKGDINPEVSMIRDIREKELKIFTDAGRVYRPLFIV
Template Known Secondary structure  TT
GGG

GGG
TT
TTS
TSS
TT
TTTT

S

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   561........570.........580.........590.........600.........610.........620.........630.........640
 
   595.. ..600.........610.........620.........630.........640..
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Query Sequence  TDG. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . VVTDEIHYLSAIEEGNYVIAQANSNLDEEGHFVEDLVTCRSKGES
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Template Sequence  EDDESLGHKELKVRKGHIAKLMATEYQDEYTWSSLLNEGLVEYIDAEEEESILIAMQPEDLEPDVDPAKRI. . . . . . . . R
Template Known Secondary structure 


TTSS
S






TTSTT

SSGGGG




TTS

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   641........650.........660.........670.........680.........690.........700.........710. .
 
   643......650.........660.........670.........680.........690.........700.........710.........720..
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Query Sequence  SLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMGANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGG
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Template Sequence  VSHHATTFTHCEIHPSMILGVAASIIPFPDHNQSPRNTYQSAMGKQAMGV. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Template Known Secondary structure 




S



S
GGGGS
TTGGGSSSGGGS
GGGB
B
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   731........740.........750.........760.........770.........780
 
   723......730.........740.........750.........760.........770.........780.........790.........800..
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Query Sequence  VVQYVDASRIVIKVNEDEMYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQNMRV
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Template Sequence  . . . . . . . . . . . . . . . FLTNYNVRMDTMANILY. . . . . . . . . . YPQKPLGTTRAMEYLK. . . . . . . . . . FRELPAGQNAIV
Template Known Secondary structure  ...............S
TTTTT


SS..........S



S
TTT..........TTTS


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   781........790....... ..800.........810... ......820.....
 
   803......810.........820.........830.........840.........850...... ...860.........870.......
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Query Sequence  AFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN. . . . . VGEAALSKLDESGIVYIGAEV
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Template Sequence  AIACYSGYNQEDSMIMNQSSIDRGLFRSLFFRSYMDQEKKYGMSITETFEKPQRTNTLRMKHGTYDKLDDDGLIAPGVRV
Template Known Secondary structure 

SSSTTTTTTTTT




S
TT


S



SSS
B

SS
GGG

TTSB

TT
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   826...830.........840.........850.........860.........870.........880.........890.........900.....
 
   878.880.........890.........900.........910.........920.........930.........940.........950.......
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Query Sequence  TGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVKDSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAK
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Template Sequence  SGEDVIIGKTTP. . . . . . . . . . . . . . . . . . . . ISSKRDASTPLRSTENGIVDQVLVTTNQDG. . . . . . . . . . . . . . . . . .
Template Known Secondary structure 
TT



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BB



TT


SSS..................
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   906...910....... ..920.........930.........940.......
 
   958.960.........970.........980.........990.........1000.........1010.........1020.........1030.......
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Query Sequence  KDLSEELQILEAGLFSRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLEAKRRKIT
Query Conservation                                                                                  
Template Conservation  ................................................................................
Template Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Template Known Secondary structure  ................................................................................
Template Predicted Secondary structure  ................................................................................
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   1038.1040.........1050.........1060.........1070.........1080.........1090.........1100.........1110.......
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Query Sequence  QGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYDENGTPVDIVLNPLGVPSRMNIGQILETHL
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Template Sequence  . . . . . . . . . LKFVKVRVRTTKIPQIGDKFASRHGQKGTIGITYRREDMPFTAEGIVPDLIINPHAIPSRMTVAHLIECLL
Template Known Secondary structure  .........



TT

TTS

TTTS
TTS


S
GGGSTTTT
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   961........970.........980.........990.........1000.........1010.........1020.........1030.
 
   1118.1120.........1130.........1140.........1150.........1160.........1170.........1180.........1190.......
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Query Sequence  GMAAKGIGDKINAMLKQQQEVAKLREFIQRAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKE
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Template Sequence  SKVAALSGNEGDASP. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . FTDITVEGISK
Template Known Secondary structure 
S


S......................................................SSS

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   1032.......1040...... ...1050.......
 
   1198.1200.........1210.........1220.........1230.........1240.........1250.........1260.........1270.......
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Query Sequence  LLKLGDLPTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGKAQFGGQRFGEMEVWA
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Template Sequence  LLREHGYQSRGFEVMYNGHTGKKLMAQIFFGPTYYQRLRHMVDDKIHARARGP. . . . . . . . . . . . . . . . GLRFGEMERDC
Template Known Secondary structure  TTTS
TTS

TTT

B
SS
BSS
GGGT







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   1058.1060.........1070.........1080.........1090.........1100.........1110 .........1120.
 
   1278.1280.........1290...... ...1300.... .....1310.........1320.........1330.........1340
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Query Sequence  LEAYGAAYTLQEMLTVKSD. . . . DVNGRTKM. . . . . . . . . . . . . YKNIVDGNHQMEPGMPESFNVLLKEIRSLGINIELE
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Template Sequence  MIAHGAASFLKERLMEASDAFRVHICGICGLMTVIAKLNHNQFECKGCDNKIDIYQIHIPYAAKLLFQELMAMNITPRLY
Template Known Secondary structure  T


SS


SSS
SS

TTTTBTTTTBSSS

TT





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   1138.1140.........1150.........1160.........1170.........1180.........1190.........1200.........1210.......
 
   1341.
Predicted Secondary structure 

Query SS confidence 

Query Sequence  DE
Query Conservation   
Alig confidence 

Template Conservation    
Template Sequence  TD
Template Known Secondary structure  S
Template Predicted Secondary structure 
Template SS confidence 

   1218.
 
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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
© Structural Bioinformatics Group, Imperial College, London
Lawrence Kelley, Benjamin Jefferys 
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