Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	M	L	S	E	E	T	S	A	V	R	P	Q	K	Q	T	R	F	N	G	A	K	L	V	W	M	L	K	G	S	P	L	T	V	T	S	A	V	I	I	V	L	M	L	L	M	M	I	F	S	P	W	L	A	T	H	D	P	N	A
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		I	D	L	T	A	R	L	L	P	P	S	A	A	H	W	F	G	T	D	E	V	G	R	D	L	F	S	R	V	L	V	G	S	Q	Q	S	I	L	A	G	L	V	V	V	A	I	A	G	M	I	G	S	L	L	G	C	L	S	G	V
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		L	G	G	R	A	D	A	I	I	M	R	I	M	D	I	M	L	S	I	P	S	L	V	L	T	M	A	L	A	A	A	L	G	P	S	L	F	N	A	M	L	A	I	A	I	V	R	I	P	F	Y	V	R	L	A	R	G	Q	A	L
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		V	V	R	Q	Y	T	Y	V	Q	A	A	K	T	F	G	A	S	R	W	H	L	I	N	W	H	I	L	R	N	S	L	P	P	L	I	V	Q	A	S	L	D	I	G	S	A	I	L	M	A	A	T	L	G	F	I	G	L	G	A	Q
Secondary structure
SS confidence
Disorder				?	?																																							?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.
Sequence		Q	P	S	A	E	W	G	A	M	V	A	N	G	R	N	Y	V	L	D	Q	W	W	Y	C	A	F	P	G	A	A	I	L	L	T	A	V	G	F	N	L	F	G	D	G	I	R	D	L	L	D	P	K	A	G	G	K	Q	S
Secondary structure
SS confidence
Disorder																																																				?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2onk chain C Region: 26 - 285 Aligned: 236 Modelled: 242 Confidence: 99.9% Identity: 17% PDB header:membrane protein Chain: C: PDB Molecule:molybdate/tungstate abc transporter, permease PDBTitle: abc transporter modbc in complex with its binding protein2 moda Phyre2 PDB 2onk chain C domain 1 Region: 26 - 285 Aligned: 236 Modelled: 242 Confidence: 99.9% Identity: 17% Fold: MetI-like Superfamily: MetI-like Family: MetI-like Phyre2 PDB 3fh6 chain F Region: 21 - 287 Aligned: 267 Modelled: 267 Confidence: 99.9% Identity: 12% PDB header:transport protein Chain: F: PDB Molecule:maltose transport system permease protein malf; PDBTitle: crystal structure of the resting state maltose transporter from e.2 coli Phyre2 PDB 3dhw chain A domain 1 Region: 89 - 283 Aligned: 187 Modelled: 195 Confidence: 99.9% Identity: 17% Fold: MetI-like Superfamily: MetI-like Family: MetI-like Phyre2 PDB 2r6g chain F domain 2 Region: 79 - 281 Aligned: 203 Modelled: 203 Confidence: 99.9% Identity: 14% Fold: MetI-like Superfamily: MetI-like Family: MetI-like Phyre2 PDB 3d31 chain C domain 1 Region: 32 - 283 Aligned: 229 Modelled: 235 Confidence: 99.9% Identity: 14% Fold: MetI-like Superfamily: MetI-like Family: MetI-like Phyre2 PDB 3d31 chain D Region: 32 - 283 Aligned: 229 Modelled: 235 Confidence: 99.9% Identity: 14% PDB header:transport protein Chain: D: PDB Molecule:sulfate/molybdate abc transporter, permease PDBTitle: modbc from methanosarcina acetivorans Phyre2 PDB 2r6g chain F Region: 88 - 281 Aligned: 194 Modelled: 194 Confidence: 99.9% Identity: 14% PDB header:hydrolase/transport protein Chain: F: PDB Molecule:maltose transport system permease protein malf; PDBTitle: the crystal structure of the e. coli maltose transporter Phyre2 PDB 2r6g chain G domain 1 Region: 28 - 283 Aligned: 254 Modelled: 256 Confidence: 99.9% Identity: 15% Fold: MetI-like Superfamily: MetI-like Family: MetI-like Phyre2 PDB 2jwa chain A Region: 94 - 127 Aligned: 34 Modelled: 34 Confidence: 29.1% Identity: 18% PDB header:transferase Chain: A: PDB Molecule:receptor tyrosine-protein kinase erbb-2; PDBTitle: erbb2 transmembrane segment dimer spatial structure Phyre2 PDB 1umq chain A Region: 173 - 204 Aligned: 32 Modelled: 32 Confidence: 9.5% Identity: 9% Fold: DNA/RNA-binding 3-helical bundle Superfamily: Homeodomain-like Family: FIS-like Phyre2 PDB 1umq chain A Region: 173 - 204 Aligned: 32 Modelled: 32 Confidence: 9.5% Identity: 9% PDB header:dna-binding protein Chain: A: PDB Molecule:photosynthetic apparatus regulatory protein; PDBTitle: solution structure and dna binding of the effector domain2 from the global regulator prra(rega) from r. sphaeroides:3 insights into dna binding specificity Phyre2 PDB 2cw1 chain A Region: 176 - 197 Aligned: 20 Modelled: 22 Confidence: 7.8% Identity: 25% PDB header:de novo protein Chain: A: PDB Molecule:sn4m; PDBTitle: solution structure of the de novo-designed lambda cro fold2 protein Phyre2 PDB 1wz4 chain A Region: 289 - 295 Aligned: 7 Modelled: 7 Confidence: 7.3% Identity: 57% PDB header:gene regulation Chain: A: PDB Molecule:major surface antigen; PDBTitle: solution conformation of adr subtype hbv pre-s2 epitope Phyre2 PDB 1ntc chain A Region: 173 - 205 Aligned: 33 Modelled: 33 Confidence: 7.1% Identity: 24% Fold: DNA/RNA-binding 3-helical bundle Superfamily: Homeodomain-like Family: FIS-like Phyre2 PDB 1eto chain B Region: 173 - 205 Aligned: 33 Modelled: 33 Confidence: 7.1% Identity: 15% Fold: DNA/RNA-binding 3-helical bundle Superfamily: Homeodomain-like Family: FIS-like Phyre2 PDB 1fip chain A Region: 173 - 205 Aligned: 33 Modelled: 33 Confidence: 7.0% Identity: 15% Fold: DNA/RNA-binding 3-helical bundle Superfamily: Homeodomain-like Family: FIS-like Phyre2 PDB 1g2h chain A Region: 189 - 205 Aligned: 17 Modelled: 17 Confidence: 5.7% Identity: 24% Fold: DNA/RNA-binding 3-helical bundle Superfamily: Homeodomain-like Family: FIS-like Phyre2 PDB 1etx chain A Region: 173 - 205 Aligned: 33 Modelled: 33 Confidence: 5.3% Identity: 15% Fold: DNA/RNA-binding 3-helical bundle Superfamily: Homeodomain-like Family: FIS-like Phyre2 1 c2onkC_ 2 d2onkc1 3 c3fh6F_ 4 d3dhwa1 5 d2r6gf2 6 d3d31c1 7 c3d31D_ 8 c2r6gF_ 9 d2r6gg1 10 c2jwaA_ 11 d1umqa_ 12 c1umqA_ 13 c2cw1A_ 14 c1wz4A_ 15 d1ntca_ 16 d1etob_ 17 d1fipa_ 18 d1g2ha_ 19 d1etxa_

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c2onkC_ 99.9 17 PDB header:membrane protein Chain: C: PDB Molecule:molybdate/tungstate abc transporter, permease PDBTitle: abc transporter modbc in complex with its binding protein2 moda 2 d2onkc1 99.9 17 Fold:MetI-like Superfamily:MetI-like Family:MetI-like 3 c3fh6F_ 99.9 12 PDB header:transport protein Chain: F: PDB Molecule:maltose transport system permease protein malf; PDBTitle: crystal structure of the resting state maltose transporter from e.2 coli 4 d3dhwa1 99.9 17 Fold:MetI-like Superfamily:MetI-like Family:MetI-like 5 d2r6gf2 99.9 14 Fold:MetI-like Superfamily:MetI-like Family:MetI-like 6 d3d31c1 99.9 14 Fold:MetI-like Superfamily:MetI-like Family:MetI-like 7 c3d31D_ 99.9 14 PDB header:transport protein Chain: D: PDB Molecule:sulfate/molybdate abc transporter, permease PDBTitle: modbc from methanosarcina acetivorans 8 c2r6gF_ 99.9 14 PDB header:hydrolase/transport protein Chain: F: PDB Molecule:maltose transport system permease protein malf; PDBTitle: the crystal structure of the e. coli maltose transporter 9 d2r6gg1 99.9 15 Fold:MetI-like Superfamily:MetI-like Family:MetI-like 10 c2jwaA_ 29.1 18 PDB header:transferase Chain: A: PDB Molecule:receptor tyrosine-protein kinase erbb-2; PDBTitle: erbb2 transmembrane segment dimer spatial structure 11 d1umqa_ 9.5 9 Fold:DNA/RNA-binding 3-helical bundle Superfamily:Homeodomain-like Family:FIS-like 12 c1umqA_ 9.5 9 PDB header:dna-binding protein Chain: A: PDB Molecule:photosynthetic apparatus regulatory protein; PDBTitle: solution structure and dna binding of the effector domain2 from the global regulator prra(rega) from r. sphaeroides:3 insights into dna binding specificity 13 c2cw1A_ 7.8 25 PDB header:de novo protein Chain: A: PDB Molecule:sn4m; PDBTitle: solution structure of the de novo-designed lambda cro fold2 protein 14 c1wz4A_ 7.3 57 PDB header:gene regulation Chain: A: PDB Molecule:major surface antigen; PDBTitle: solution conformation of adr subtype hbv pre-s2 epitope 15 d1ntca_ 7.1 24 Fold:DNA/RNA-binding 3-helical bundle Superfamily:Homeodomain-like Family:FIS-like 16 d1etob_ 7.1 15 Fold:DNA/RNA-binding 3-helical bundle Superfamily:Homeodomain-like Family:FIS-like 17 d1fipa_ 7.0 15 Fold:DNA/RNA-binding 3-helical bundle Superfamily:Homeodomain-like Family:FIS-like 18 d1g2ha_ 5.7 24 Fold:DNA/RNA-binding 3-helical bundle Superfamily:Homeodomain-like Family:FIS-like 19 d1etxa_ 5.3 15 Fold:DNA/RNA-binding 3-helical bundle Superfamily:Homeodomain-like Family:FIS-like

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0