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Job DescriptionP26612
Confidence100.00%DateThu Jan 5 11:42:59 GMT 2012
Rank42Aligned Residues412
% Identity20%Templatec2ya0A_
PDB info PDB header:hydrolaseChain: A: PDB Molecule:putative alkaline amylopullulanase; PDBTitle: catalytic module of the multi-modular glycogen-degrading2 pneumococcal virulence factor spua
Resolution1.85 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   3......10........ .20.........30.........40......... 50.....
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Query Sequence  NPTLLQCFHWYYPEGG. . . . . . . . . . KLWPELAERADGFNDIGINMVWLPPAYKGAS. . . . . . . . . . . . . . . . . GGYSVG
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Template Sequence  DAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVLSYYFVNELKNHERLSDYASSNSNYNWG
Template Known Secondary structure  G




GGGTTT
SS
TTSTTT
SS

B
BSS
BGGGTTS


S

SSS
S


S
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   132.......140.........150.........160.........170.........180.........190.........200.........210.
 
   56...60.........70......... 80.........90.........100.........110.........120.......
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Query Sequence  YDSYDLFDLGEFDQKGSIPTKYGD. . . . . . . . KAQLLAAIDALKRNDIAVLLDVVVNHKMGADEKEAIRVQRVNADDRTQ
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Template Sequence  YDPQNYF. . . . . . . . . SLTGMYSSDPKNPEKRIAEFKNLINEIHKRGMGAILDVVYNHTA. . . . . . . . . . . . . . . . . . . .
Template Known Secondary structure 
SBS
SS.........SB
STTSS
TTSTTTT

TTB
S....................
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   212...... .220.........230.........240.........250.........260..
 
   128.130.........140.........150.........160.........170.........180.........190.........200.......
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Query Sequence  IDEEIIECEGWTRYTFPARAGQYSQFIWDFKCFSGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDF
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Template Sequence  . . . . . . . . . . . . . . . . . . KVDLFEDLEPNYYHFMDADGTPRT. . . . . . . . . . . . . . . . . . . . . . . . . . . . . SFGGGRLGT
Template Known Secondary structure  ..................
TTSTTTSB
B
TT

B
.............................TTBB
T
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   263......270.........280...... ...290.....
 
   208.210.........220.........230.........240.........250.........260.........270.........280.....
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Query Sequence  RNHAVTEEIKYWARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQTYIDQVEGKT. .
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Template Sequence  THHMTKRLLIDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNP. NLIMLGEGWRTYAGDENMPTKAADQDWMK
Template Known Secondary structure  TS


TTGGGSB
T.T



S



STT



BSSGGGGG
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   296...300.........310.........320.........330.........340..... ....350.........360.........370....
 
   286...290........ .300.........310....... ..320.........330.........340
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Query Sequence  . . . . . MLFDAPLQMKFHE. . . . . . . . ASRMGRDYDMTQIFTGTLV. . . . . . EADPFHAVTLVANHDTQPLQALE. . . . . .
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Template Sequence  HTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKNLIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKK
Template Known Secondary structure  G
SSS


SSSTT


TTTT

TT

TTS

SSGGG
S

SSSS
T
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   375....380.........390.........400.........410.........420.........430.........440.........450....
 
   341........350.........360.........370.........380.......
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Query Sequence  . . . . . . APVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPI. . . . . . . . . . . . . . . . . . . . . . . . . . .
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Template Sequence  DPSKAENYAEIHRRLRLGNLMVLTAQGTPFIHSGQEYGRTKQFRNPAYRTPVAEDKVPNKSHLLRDKDGNPFDYPYFIHD
Template Known Secondary structure 
TTSSSSTTTTTT


B


GGGSS


GGG

TT
TT

S
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   455....460.........470.........480.........490.........500.........510.........520.........530....
 
   388.390.........400.........410... ......420.....
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Query Sequence  . . . . . . . . . . . . . . . . . . . . . . . . DMPIIEQLDELILARQRFAHGVQTLF. . . . . . . . . . . . . . . . . . FDHPNCIAFSRS
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Template Sequence  SYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDRVHLITVPGQNGVEKEDVVIGYQIT
Template Known Secondary structure 
TT





TTT
T
GGGG
SSSS
TTSTT

SS
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   426...430.........440.........450.........460.........470....... ..480.........490...
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Query Sequence  GTDEFPGCVVVMSNGDDGEKTIHLGENYGNKTWRDFLGNRQERVVTDENGEA. . . . . . . TFFCNGGSVSVWVIEE
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Template Sequence  APNGD. . IYAVFVNADEKAREFNLGTAFAHLRNAEVLADENQAGPVGIANPKGLEWTEKGLKLNALTATVLRVSQ
Template Known Secondary structure 
TTS
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SSS

TTTTGGGGG

SS
B
SS

SS
SS
SS
TT
G
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No model constructed - rank, confidence too low




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Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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