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Job DescriptionP26612
Confidence100.00%DateThu Jan 5 11:42:59 GMT 2012
Rank31Aligned Residues420
% Identity19%Templatec2wcsA_
PDB info PDB header:hydrolaseChain: A: PDB Molecule:alpha amylase, catalytic region; PDBTitle: crystal structure of debranching enzyme from nostoc2 punctiforme (npde)
Resolution2.80 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   1........10.... .....20.........30.........40.........50....
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Query Sequence  MRNPTLLQCFHWYY. . . . . . . . . . . . . . . . . . . . . . . . . . PEGGKLWPELAERADGFNDIGINMVWLPPAYKGASGGYSV
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Template Sequence  VKHAVFYQIFPDRFARSKQPRKRLLQEARWEDWDSMPTLQGYKGGDLWGIMEDLDYIQNLGINAIYFTPIFQSAS. . . NH
Template Known Secondary structure  GGG


GGGT


SS



S
TT

TTTGGG
T

S

TT

S

ST...T
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   910.........20.........30.........40.........50.........60.........70.........80... ..
 
   55....60.........70.........80.........90.........100.........110.........120.........130....
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Query Sequence  GYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVVVNHKMGADEKEAIRVQRVNADDRTQIDEEIIE
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Template Sequence  RYHTHDYY. . . . . . . . . QVDPMLGGNEAFKELLDAAHQRNIKVVLDGVFNHSS. . RGFFFFHDVLEN. . . . . . . . . . . . .
Template Known Secondary structure  TTS.........
GGGTTT


SB

..TTS.............
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   86...90... ......100.........110.........120......... 130.........140.
 
   135....140.........150.........160.........170.........180.........190.........200.........210....
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Query Sequence  CEGWTRYTFPARAGQYSQFIWDFKCFSGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTE
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Template Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . GPHSPWVNWFKIEGWPLSPYNGEFPANYVGWAGNRALPEFNHDNPEVRE
Template Known Secondary structure  ...............................GGG
TTGGGB
B
SSS

SS

SS

SB
BGGG
TTSBBB
TTS
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   142.......150.........160.........170.........180.........190
 
   215....220.........230.........240. ........250.........260.........270.........280.........290...
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Query Sequence  EIKYWARWVMEQTQCDGFRLDAVKHIP. AWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQTYIDQVEGKTMLFDAPLQ
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Template Sequence  YIMEIAEYWL. KFGIDGWRLDVPFEIKTPGFWQEFRDRTKAINP. EAYIVGEVWGDSRQWLDG. . . . . TQFDGVMNYLFA
Template Known Secondary structure  .T

STTS


TT
T.T


S

GGG
SS.....SS
S
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   191........200 .........210.........220.........230... ......240.........250. ........260...
 
   294.....300.. .......310.........320.........330.........340.........350..
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Query Sequence  MKFHEASRM. . . . . . . . . . . . . . . . . . . . . GRDYDMTQIFTGTLVEADPFHAVTLVANHDTQPLQALEAPVEPWFKPLAY
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Template Conservation                                                             
                    
Template Sequence  GPTIAFAAGDRVVLEQVQSRDYQPYPPLFAAEYATKIQEVLQLYPWEIQLTQLNLLASHDTARLMTIAGGDIASVELSTL
Template Known Secondary structure  TGGG

SSS

TTT



S


TSS
TT

S

TTS

TTT
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   264.....270.........280.........290.........300.........310.........320.........330.........340...
 
   353......360.........370.........380....... ..390.........400....... ..410.........420......
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Query Sequence  ALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPI. . . DMPIIEQLDELILARQRFAH. . . GVQTLFFDHPNCIAFSRSG
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Template Sequence  . . LLLTFPGAPSIYYGDEVGLPGGIDPDSRRGFPEANWNQEIFNTHRQLITIRQTYPALRTGDYQVLYAQGQLYLFARTL
Template Known Secondary structure  ..TTSSSTTGGGT



SSSSS










TSTT
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   344.....350.........360.........370.........380.........390.........400.........410.........420.
 
   427..430.........440.........450.........460.........470... ......480.........490
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Query Sequence  TDEFPGCVVVMSNGDDGEKTIHLGENYGNKTWRDFLGNRQERVVTDE. . NGEATFFCNGGSVSVWV
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Template Sequence  GTE. . . ELIIAINAGTSSATANVDVASLHTQPNKLLYGTAEAEWNGEEGTQQLSLTLPARSGCILG
Template Known Secondary structure  TT...
SSS



S
SS


S




STT


SS
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   423.. ....430.........440.........450.........460.........470.........480.....
 
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No model constructed - rank, confidence too low




Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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