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Job DescriptionP26612
Confidence100.00%DateThu Jan 5 11:42:59 GMT 2012
Rank46Aligned Residues412
% Identity18%Templatec2e8yA_
PDB info PDB header:hydrolaseChain: A: PDB Molecule:amyx protein; PDBTitle: crystal structure of pullulanase type i from bacillus subtilis str.2 168
Resolution2.11 Å

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   4.....10........ .20....... ..30.........40......... 50.........60..
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Query Sequence  PTLLQCFHWYYPEGG. . . KLWPELAER. . . . . . . . . . . ADGFNDIGINMVWLPPAYKGAS. . . . . . . GGYSVGYDSYDLF
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Template Sequence  ETHLRDFSIHENSGMINKGKYLALTETDTQTANGSSSGLAYVKELGVTHVELLPVNDFAGVDEEKPLDAYNWGYNPLHFF
Template Known Secondary structure 
STTS

SSTTSGGGGG
TT
B
TTS
B
T
SS

SSS
TTSGGG





S
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   219220.........230.........240.........250.........260.........270.........280.........290........
 
   63......70......... 80.........90.........100.........110.........120.........130....
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Query Sequence  DLGEFDQKGSIPTKYGD. . . . . . . . KAQLLAAIDALKRNDIAVLLDVVVNHKMGADEKEAIRVQRVNADDRTQIDEEIIE
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Template Sequence  . . . . . . . . . APEGSYASNPHDPQTRKTELKQMINTLHQHGLRVILDVVFNHVYKRENS. . . . . . . . . . . . . . . . . . . . . .
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STTSS
SSSTT

TT

SSGGGS......................
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   299300.........310.........320.........330.........340.......
 
   135....140.........150.........160.........170.........180.........190.........200.........210....
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Query Sequence  CEGWTRYTFPARAGQYSQFIWDFKCFSGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTE
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Template Sequence  . . . . . . . . . . . . . . PFEKTVPGYFFRHDECGKPSN. . . . . . . . . . . . . . . . . . . . . . . . . . . . GTGVGNDIASERRMARK
Template Known Secondary structure  ..............STTTSB
B
TTSSB

............................TTSSS

B
TTS
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   348.350.........360........ .370.........380.....
 
   215....220.........230.........240.........250.........260.........270.........280..... ....
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Query Sequence  EIKYWARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQTYIDQVEGKT. . . . . MLFD
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Template Sequence  FIADCVVYWLEEYNVDGFRFDLLGILDIDTVLYMKEKATKAKP. GILLFGEGWDLATPLPHEQKAALANAPRMPGIGFFN
Template Known Secondary structure 


TTGGGSBST.T







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GGGB

GGGGGG
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   386...390.........400.........410.........420........ .430.........440.........450.........460....
 
   290........ .300.........310........ .320.........330.........340.....
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Query Sequence  APLQMKFHE. . . . . . . . ASRMGRDYDMTQIFTGTLVE. . . . . . . . . . ADPFHAVTLVANHDTQPLQALEAPVEP. . . . . .
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Template Sequence  DMFRDAVKGNTFHLKATGFALGNGESAQAVMHGIAGSSGWKALAPIVPEPSQSINYVESHDNHTFWDKMSFALPQENDSR
Template Known Secondary structure 

SSSTT


GGGT
GGGBTTS
BTTB

SSSSGGG
S

SSSS

TT

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   465....470.........480.........490.........500.........510.........520.........530.........540....
 
   346...350.........360.........370........ .380.........390.........400.........
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Query Sequence  . WFKPLAYALILLRENGVPSVFYPDLYGAHYEDV. . . . . . . . . . . GGDGQTYPIDMPIIEQLDELILARQRFAHGV. . . .
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Template Sequence  KRSRQRLAVAIILLAQGVPFIHSGQEFFRTKQGVENSYQSSDSINQLDWDRRETFKEDVHYIRRLISLRKAHPAFRLRSA
Template Known Secondary structure  TSBSTTGGGT


TT
S

TT

S

T
GGGG

S
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   545....550.........560.........570.........580.........590.........600.........610.........620....
 
   410.........420.........430.. .......440.........450.........460.........470.........480.
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Query Sequence  . . . . . . QTLFFDHPNCIAFSRSGTDEFPG. . CVVVMSNGDDGEKTIHLGENYGNKTWRDFLGNRQERVVTDENGEATFFC
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Template Sequence  ADIQRHLECLTLKEHLIAYRLYDLDEVDEWKDIIVIHHASPDSVEWRL. . . PNDIPYRLLCDPSGFQEDPTEIKK. TVAV
Template Known Secondary structure 
SS


TTT
SSS
SSS
...SS
S
TT
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   625....630.........640.........650.........660.........670.. .......680.........690...... ...700
 
   482.......490...
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Query Sequence  NGGSVSVWVIEE
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Template Sequence  NGIGTVILYLAS
Template Known Secondary structure  SS

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   701........710..
 
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No model constructed - rank, confidence too low




Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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