Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	M	M	M	Q	S	R	K	I	W	Y	Y	R	I	T	L	I	I	L	L	F	A	M	L	L	A	W	A	L	L	P	G	V	H	E	F	I	N	R	S	V	A	A	F	A	A	V	D	Q	Q	G	I	E	R	F	I	Q	S	Y	G
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?																																			?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		A	L	A	A	V	V	S	F	L	L	M	I	L	Q	A	I	A	A	P	L	P	A	F	L	I	T	F	A	N	A	S	L	F	G	A	F	W	G	G	L	L	S	W	T	S	S	M	A	G	A	A	L	C	F	F	I	A	R	V	M
Secondary structure
SS confidence
Disorder																			?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		G	R	E	V	V	E	K	L	T	G	K	T	V	L	D	S	M	D	G	F	F	T	R	Y	G	K	H	T	I	L	V	C	R	L	L	P	F	V	P	F	D	P	I	S	Y	A	A	G	L	T	S	I	R	F	R	S	F	F	I	A
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.
Sequence		T	G	L	G	Q	L	P	A	T	I	V	Y	S	W	A	G	S	M	L	T	G	G	T	F	W	F	V	T	G	L	F	I	L	F	A	L	T	V	V	I	F	M	A	K	K	I	W	L	E	R	Q	K	R	N	A
Secondary structure
SS confidence
Disorder																									?																												?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 3p5n chain A Region: 81 - 112 Aligned: 32 Modelled: 32 Confidence: 69.6% Identity: 9% PDB header:transport protein Chain: A: PDB Molecule:riboflavin uptake protein; PDBTitle: structure and mechanism of the s component of a bacterial ecf2 transporter Phyre2 PDB 3qbr chain A Region: 11 - 66 Aligned: 56 Modelled: 56 Confidence: 39.4% Identity: 11% PDB header:apoptosis Chain: A: PDB Molecule:sjchgc06286 protein; PDBTitle: bakbh3 in complex with sja Phyre2 PDB 1f16 chain A Region: 3 - 66 Aligned: 64 Modelled: 64 Confidence: 36.6% Identity: 17% Fold: Toxins' membrane translocation domains Superfamily: Bcl-2 inhibitors of programmed cell death Family: Bcl-2 inhibitors of programmed cell death Phyre2 PDB 1bxl chain A Region: 11 - 66 Aligned: 56 Modelled: 56 Confidence: 35.4% Identity: 20% Fold: Toxins' membrane translocation domains Superfamily: Bcl-2 inhibitors of programmed cell death Family: Bcl-2 inhibitors of programmed cell death Phyre2 PDB 1ysg chain A domain 1 Region: 11 - 66 Aligned: 56 Modelled: 56 Confidence: 31.1% Identity: 20% Fold: Toxins' membrane translocation domains Superfamily: Bcl-2 inhibitors of programmed cell death Family: Bcl-2 inhibitors of programmed cell death Phyre2 PDB 1o0l chain A Region: 11 - 66 Aligned: 56 Modelled: 56 Confidence: 29.0% Identity: 14% Fold: Toxins' membrane translocation domains Superfamily: Bcl-2 inhibitors of programmed cell death Family: Bcl-2 inhibitors of programmed cell death Phyre2 PDB 2o2f chain A Region: 11 - 66 Aligned: 56 Modelled: 56 Confidence: 28.3% Identity: 13% PDB header:apoptosis Chain: A: PDB Molecule:apoptosis regulator bcl-2; PDBTitle: solution structure of the anti-apoptotic protein bcl-2 in2 complex with an acyl-sulfonamide-based ligand Phyre2 PDB 2pon chain B domain 1 Region: 11 - 69 Aligned: 59 Modelled: 59 Confidence: 26.7% Identity: 19% Fold: Toxins' membrane translocation domains Superfamily: Bcl-2 inhibitors of programmed cell death Family: Bcl-2 inhibitors of programmed cell death Phyre2 PDB 3pk1 chain A Region: 3 - 69 Aligned: 67 Modelled: 67 Confidence: 26.7% Identity: 12% PDB header:apoptosis/apoptosis regulator Chain: A: PDB Molecule:induced myeloid leukemia cell differentiation protein mcl- PDBTitle: crystal structure of mcl-1 in complex with the baxbh3 domain Phyre2 PDB 1g5m chain A Region: 11 - 69 Aligned: 59 Modelled: 59 Confidence: 26.3% Identity: 12% Fold: Toxins' membrane translocation domains Superfamily: Bcl-2 inhibitors of programmed cell death Family: Bcl-2 inhibitors of programmed cell death Phyre2 PDB 1pq1 chain A Region: 11 - 69 Aligned: 59 Modelled: 59 Confidence: 25.3% Identity: 17% Fold: Toxins' membrane translocation domains Superfamily: Bcl-2 inhibitors of programmed cell death Family: Bcl-2 inhibitors of programmed cell death Phyre2 PDB 2xa0 chain A Region: 11 - 66 Aligned: 56 Modelled: 56 Confidence: 25.2% Identity: 13% PDB header:apoptosis Chain: A: PDB Molecule:apoptosis regulator bcl-2; PDBTitle: crystal structure of bcl-2 in complex with a bax bh32 peptide Phyre2 PDB 1zy3 chain A domain 1 Region: 11 - 68 Aligned: 58 Modelled: 58 Confidence: 23.8% Identity: 16% Fold: Toxins' membrane translocation domains Superfamily: Bcl-2 inhibitors of programmed cell death Family: Bcl-2 inhibitors of programmed cell death Phyre2 PDB 2jm6 chain B domain 1 Region: 11 - 69 Aligned: 59 Modelled: 59 Confidence: 22.0% Identity: 15% Fold: Toxins' membrane translocation domains Superfamily: Bcl-2 inhibitors of programmed cell death Family: Bcl-2 inhibitors of programmed cell death Phyre2 PDB 2yv6 chain A Region: 11 - 62 Aligned: 52 Modelled: 52 Confidence: 21.8% Identity: 15% PDB header:apoptosis Chain: A: PDB Molecule:bcl-2 homologous antagonist/killer; PDBTitle: crystal structure of human bcl-2 family protein bak Phyre2 PDB 2bbj chain B Region: 131 - 232 Aligned: 70 Modelled: 71 Confidence: 20.8% Identity: 23% PDB header:metal transport/membrane protein Chain: B: PDB Molecule:divalent cation transport-related protein; PDBTitle: crystal structure of the cora mg2+ transporter Phyre2 1 c3p5nA_ 2 c3qbrA_ 3 d1f16a_ 4 d1bxla_ 5 d1ysga1 6 d1o0la_ 7 c2o2fA_ 8 d2ponb1 9 c3pk1A_ 10 d1g5ma_ 11 d1pq1a_ 12 c2xa0A_ 13 d1zy3a1 14 d2jm6b1 15 c2yv6A_ 16 c2bbjB_

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c3p5nA_ 69.6 9 PDB header:transport protein Chain: A: PDB Molecule:riboflavin uptake protein; PDBTitle: structure and mechanism of the s component of a bacterial ecf2 transporter 2 c3qbrA_ 39.4 11 PDB header:apoptosis Chain: A: PDB Molecule:sjchgc06286 protein; PDBTitle: bakbh3 in complex with sja 3 d1f16a_ 36.6 17 Fold:Toxins' membrane translocation domains Superfamily:Bcl-2 inhibitors of programmed cell death Family:Bcl-2 inhibitors of programmed cell death 4 d1bxla_ 35.4 20 Fold:Toxins' membrane translocation domains Superfamily:Bcl-2 inhibitors of programmed cell death Family:Bcl-2 inhibitors of programmed cell death 5 d1ysga1 31.1 20 Fold:Toxins' membrane translocation domains Superfamily:Bcl-2 inhibitors of programmed cell death Family:Bcl-2 inhibitors of programmed cell death 6 d1o0la_ 29.0 14 Fold:Toxins' membrane translocation domains Superfamily:Bcl-2 inhibitors of programmed cell death Family:Bcl-2 inhibitors of programmed cell death 7 c2o2fA_ 28.3 13 PDB header:apoptosis Chain: A: PDB Molecule:apoptosis regulator bcl-2; PDBTitle: solution structure of the anti-apoptotic protein bcl-2 in2 complex with an acyl-sulfonamide-based ligand 8 d2ponb1 26.7 19 Fold:Toxins' membrane translocation domains Superfamily:Bcl-2 inhibitors of programmed cell death Family:Bcl-2 inhibitors of programmed cell death 9 c3pk1A_ 26.7 12 PDB header:apoptosis/apoptosis regulator Chain: A: PDB Molecule:induced myeloid leukemia cell differentiation protein mcl- PDBTitle: crystal structure of mcl-1 in complex with the baxbh3 domain 10 d1g5ma_ 26.3 12 Fold:Toxins' membrane translocation domains Superfamily:Bcl-2 inhibitors of programmed cell death Family:Bcl-2 inhibitors of programmed cell death 11 d1pq1a_ 25.3 17 Fold:Toxins' membrane translocation domains Superfamily:Bcl-2 inhibitors of programmed cell death Family:Bcl-2 inhibitors of programmed cell death 12 c2xa0A_ 25.2 13 PDB header:apoptosis Chain: A: PDB Molecule:apoptosis regulator bcl-2; PDBTitle: crystal structure of bcl-2 in complex with a bax bh32 peptide 13 d1zy3a1 23.8 16 Fold:Toxins' membrane translocation domains Superfamily:Bcl-2 inhibitors of programmed cell death Family:Bcl-2 inhibitors of programmed cell death 14 d2jm6b1 22.0 15 Fold:Toxins' membrane translocation domains Superfamily:Bcl-2 inhibitors of programmed cell death Family:Bcl-2 inhibitors of programmed cell death 15 c2yv6A_ 21.8 15 PDB header:apoptosis Chain: A: PDB Molecule:bcl-2 homologous antagonist/killer; PDBTitle: crystal structure of human bcl-2 family protein bak 16 c2bbjB_ 20.8 23 PDB header:metal transport/membrane protein Chain: B: PDB Molecule:divalent cation transport-related protein; PDBTitle: crystal structure of the cora mg2+ transporter

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0