Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	K	P	M	H	I	A	M	A	L	L	S	A	A	M	F	F	V	L	A	G	V	F	M	G	V	Q	L	E	L	D	G	T	K	L	V	V	D	T	A	S	D	V	R	W	Q	W	V	F	I	G	T	A	V	V	F	F	F	Q	L
Secondary structure
SS confidence
Disorder		?	?	?	?																					?	?	?	?	?		?	?																												?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		L	R	P	A	F	Q	K	G	L	K	S	V	S	G	P	K	F	I	L	P	A	I	D	G	S	T	V	K	Q	K	L	F	L	V	A	L	L	V	L	A	V	A	W	P	F	M	V	S	R	G	T	V	D	I	A	T	L	T	M	I
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		Y	I	I	L	G	L	G	L	N	V	V	V	G	L	S	G	L	L	V	L	G	Y	G	G	F	Y	A	I	G	A	Y	T	F	A	L	L	N	H	Y	Y	G	L	G	F	W	T	C	L	P	I	A	G	L	M	A	A	A	A	G	F
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		L	L	G	F	P	V	L	R	L	R	G	D	Y	L	A	I	V	T	L	G	F	G	E	I	V	R	I	L	L	L	N	N	T	E	I	T	G	G	P	N	G	I	S	Q	I	P	K	P	T	L	F	G	L	E	F	S	R	T	A	R
Secondary structure
SS confidence
Disorder																																			?							?	?	?		?	?	?	?	?	?	?			?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		E	G	G	W	D	T	F	S	N	F	F	G	L	K	Y	D	P	S	D	R	V	I	F	L	Y	L	V	A	L	L	L	V	V	L	S	L	F	V	I	N	R	L	L	R	M	P	L	G	R	A	W	E	A	L	R	E	D	E	I	A
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?		?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		C	R	S	L	G	L	S	P	R	R	I	K	L	T	A	F	T	I	S	A	A	F	A	G	F	A	G	T	L	F	A	A	R	Q	G	F	V	S	P	E	S	F	T	F	A	E	S	A	F	V	L	A	I	V	V	L	G	G	M	G
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		S	Q	F	A	V	I	L	A	A	I	L	L	V	V	S	R	E	L	M	R	D	F	N	E	Y	S	M	L	M	L	G	G	L	M	V	L	M	M	I	W	R	P	Q	G	L	L	P	M	T	R	P	Q	L	K	L	K	N	G	A	A
Secondary structure
SS confidence
Disorder																																																							?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.
Sequence		K	G	E	Q	A
Secondary structure
SS confidence
Disorder		?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2nq2 chain A Region: 149 - 326 Aligned: 148 Modelled: 154 Confidence: 97.7% Identity: 13% PDB header:metal transport Chain: A: PDB Molecule:hypothetical abc transporter permease protein PDBTitle: an inward-facing conformation of a putative metal-chelate2 type abc transporter. Phyre2 PDB 1l7v chain A Region: 140 - 326 Aligned: 156 Modelled: 159 Confidence: 96.2% Identity: 17% Fold: ABC transporter involved in vitamin B12 uptake, BtuC Superfamily: ABC transporter involved in vitamin B12 uptake, BtuC Family: ABC transporter involved in vitamin B12 uptake, BtuC Phyre2 PDB 1pv7 chain A Region: 307 - 414 Aligned: 105 Modelled: 108 Confidence: 28.8% Identity: 10% Fold: MFS general substrate transporter Superfamily: MFS general substrate transporter Family: LacY-like proton/sugar symporter Phyre2 PDB 2e74 chain G domain 1 Region: 317 - 342 Aligned: 26 Modelled: 26 Confidence: 23.0% Identity: 27% Fold: Single transmembrane helix Superfamily: PetG subunit of the cytochrome b6f complex Family: PetG subunit of the cytochrome b6f complex Phyre2 PDB 3pxp chain A Region: 282 - 314 Aligned: 33 Modelled: 32 Confidence: 22.3% Identity: 30% PDB header:transcription regulator Chain: A: PDB Molecule:helix-turn-helix domain protein; PDBTitle: crystal structure of a pas and dna binding domain containing protein2 (caur_2278) from chloroflexus aurantiacus j-10-fl at 2.30 a3 resolution Phyre2 PDB 2xut chain C Region: 313 - 424 Aligned: 112 Modelled: 112 Confidence: 13.9% Identity: 6% PDB header:transport protein Chain: C: PDB Molecule:proton/peptide symporter family protein; PDBTitle: crystal structure of a proton dependent oligopeptide (pot)2 family transporter. Phyre2 PDB 4a18 chain U Region: 291 - 309 Aligned: 19 Modelled: 19 Confidence: 8.1% Identity: 16% PDB header:ribosome Chain: U: PDB Molecule:rpl13; PDBTitle: t.thermophila 60s ribosomal subunit in complex with initiation2 factor 6. this file contains 26s rrna and proteins of molecule 1 Phyre2 PDB 1vf5 chain G Region: 317 - 336 Aligned: 20 Modelled: 20 Confidence: 7.9% Identity: 30% PDB header:photosynthesis Chain: G: PDB Molecule:protein pet g; PDBTitle: crystal structure of cytochrome b6f complex from m.laminosus Phyre2 PDB 1vf5 chain G Region: 317 - 336 Aligned: 20 Modelled: 20 Confidence: 7.9% Identity: 30% Fold: Single transmembrane helix Superfamily: PetG subunit of the cytochrome b6f complex Family: PetG subunit of the cytochrome b6f complex Phyre2 PDB 3u5e chain L Region: 291 - 309 Aligned: 19 Modelled: 19 Confidence: 7.6% Identity: 16% PDB header:ribosome Chain: L: PDB Molecule:60s ribosomal protein l13-a; PDBTitle: the structure of the eukaryotic ribosome at 3.0 resolution Phyre2 PDB 1rp1 chain A domain 2 Region: 298 - 327 Aligned: 30 Modelled: 30 Confidence: 7.2% Identity: 27% Fold: alpha/beta-Hydrolases Superfamily: alpha/beta-Hydrolases Family: Pancreatic lipase, N-terminal domain Phyre2 PDB 1y9q chain A Region: 284 - 314 Aligned: 31 Modelled: 26 Confidence: 6.9% Identity: 13% PDB header:transcription regulator Chain: A: PDB Molecule:transcriptional regulator, hth_3 family; PDBTitle: crystal structure of hth_3 family transcriptional regulator2 from vibrio cholerae Phyre2 PDB 1y6u chain A Region: 298 - 315 Aligned: 18 Modelled: 18 Confidence: 5.7% Identity: 17% PDB header:dna binding protein Chain: A: PDB Molecule:excisionase from transposon tn916; PDBTitle: the structure of the excisionase (xis) protein from2 conjugative transposon tn916 provides insights into the3 regulation of heterobivalent tyrosine recombinases Phyre2 PDB 1hpl chain A domain 2 Region: 298 - 327 Aligned: 30 Modelled: 30 Confidence: 5.5% Identity: 23% Fold: alpha/beta-Hydrolases Superfamily: alpha/beta-Hydrolases Family: Pancreatic lipase, N-terminal domain Phyre2 PDB 1eth chain A domain 2 Region: 298 - 327 Aligned: 30 Modelled: 30 Confidence: 5.2% Identity: 33% Fold: alpha/beta-Hydrolases Superfamily: alpha/beta-Hydrolases Family: Pancreatic lipase, N-terminal domain Phyre2 1 c2nq2A_ 2 d1l7va_ 3 d1pv7a_ 4 d2e74g1 5 c3pxpA_ 6 c2xutC_ 7 c4a18U_ 8 c1vf5G_ 9 d1vf5g_ 10 c3u5eL_ 11 d1rp1a2 12 c1y9qA_ 13 c1y6uA_ 14 d1hpla2 15 d1etha2

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c2nq2A_ 97.7 13 PDB header:metal transport Chain: A: PDB Molecule:hypothetical abc transporter permease protein PDBTitle: an inward-facing conformation of a putative metal-chelate2 type abc transporter. 2 d1l7va_ 96.2 17 Fold:ABC transporter involved in vitamin B12 uptake, BtuC Superfamily:ABC transporter involved in vitamin B12 uptake, BtuC Family:ABC transporter involved in vitamin B12 uptake, BtuC 3 d1pv7a_ 28.8 10 Fold:MFS general substrate transporter Superfamily:MFS general substrate transporter Family:LacY-like proton/sugar symporter 4 d2e74g1 23.0 27 Fold:Single transmembrane helix Superfamily:PetG subunit of the cytochrome b6f complex Family:PetG subunit of the cytochrome b6f complex 5 c3pxpA_ 22.3 30 PDB header:transcription regulator Chain: A: PDB Molecule:helix-turn-helix domain protein; PDBTitle: crystal structure of a pas and dna binding domain containing protein2 (caur_2278) from chloroflexus aurantiacus j-10-fl at 2.30 a3 resolution 6 c2xutC_ 13.9 6 PDB header:transport protein Chain: C: PDB Molecule:proton/peptide symporter family protein; PDBTitle: crystal structure of a proton dependent oligopeptide (pot)2 family transporter. 7 c4a18U_ 8.1 16 PDB header:ribosome Chain: U: PDB Molecule:rpl13; PDBTitle: t.thermophila 60s ribosomal subunit in complex with initiation2 factor 6. this file contains 26s rrna and proteins of molecule 1 8 c1vf5G_ 7.9 30 PDB header:photosynthesis Chain: G: PDB Molecule:protein pet g; PDBTitle: crystal structure of cytochrome b6f complex from m.laminosus 9 d1vf5g_ 7.9 30 Fold:Single transmembrane helix Superfamily:PetG subunit of the cytochrome b6f complex Family:PetG subunit of the cytochrome b6f complex 10 c3u5eL_ 7.6 16 PDB header:ribosome Chain: L: PDB Molecule:60s ribosomal protein l13-a; PDBTitle: the structure of the eukaryotic ribosome at 3.0 resolution 11 d1rp1a2 7.2 27 Fold:alpha/beta-Hydrolases Superfamily:alpha/beta-Hydrolases Family:Pancreatic lipase, N-terminal domain 12 c1y9qA_ 6.9 13 PDB header:transcription regulator Chain: A: PDB Molecule:transcriptional regulator, hth_3 family; PDBTitle: crystal structure of hth_3 family transcriptional regulator2 from vibrio cholerae 13 c1y6uA_ 5.7 17 PDB header:dna binding protein Chain: A: PDB Molecule:excisionase from transposon tn916; PDBTitle: the structure of the excisionase (xis) protein from2 conjugative transposon tn916 provides insights into the3 regulation of heterobivalent tyrosine recombinases 14 d1hpla2 5.5 23 Fold:alpha/beta-Hydrolases Superfamily:alpha/beta-Hydrolases Family:Pancreatic lipase, N-terminal domain 15 d1etha2 5.2 33 Fold:alpha/beta-Hydrolases Superfamily:alpha/beta-Hydrolases Family:Pancreatic lipase, N-terminal domain

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0