Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	T	L	P	S	G	H	P	K	S	R	L	I	K	K	F	T	A	L	G	P	Y	I	R	E	G	K	C	K	D	N	R	F	F	F	D	C	L	A	V	C	V	N	V	K	P	A	P	E	V	R	E	F	W	G	W	W	M	E	L
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?																								?													?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		E	A	Q	E	S	R	F	T	Y	S	Y	Q	F	G	L	F	D	K	A	G	D	W	K	S	V	P	V	K	D	T	E	V	V	E	R	L	E	H	T	L	R	E	F	H	E	K	L	R	E	L	L	T	T	L	N	L	K	L	E	P
Secondary structure
SS confidence
Disorder																						?								?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.
Sequence		A	D	D	F	R	D	E	P	V	K	L	T	A
Secondary structure
SS confidence
Disorder		?			?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 3rpj chain A Region: 7 - 131 Aligned: 124 Modelled: 125 Confidence: 100.0% Identity: 48% PDB header:transcription regulator Chain: A: PDB Molecule:curlin genes transcriptional regulator; PDBTitle: structure of a curlin genes transcriptional regulator protein from2 proteus mirabilis hi4320. Phyre2 PDB 2bhu chain A domain 1 Region: 38 - 86 Aligned: 44 Modelled: 49 Confidence: 67.4% Identity: 20% Fold: Immunoglobulin-like beta-sandwich Superfamily: E set domains Family: E-set domains of sugar-utilizing enzymes Phyre2 PDB 2b9b chain A Region: 47 - 55 Aligned: 9 Modelled: 9 Confidence: 62.2% Identity: 44% PDB header:viral protein Chain: A: PDB Molecule:fusion glycoprotein f0; PDBTitle: structure of the parainfluenza virus 5 f protein in its metastable,2 pre-fusion conformation Phyre2 PDB 2a1v chain A domain 1 Region: 41 - 121 Aligned: 74 Modelled: 81 Confidence: 49.9% Identity: 18% Fold: Secretion chaperone-like Superfamily: YjbR-like Family: YjbR-like Phyre2 PDB 3due chain A domain 1 Region: 56 - 102 Aligned: 47 Modelled: 47 Confidence: 45.9% Identity: 23% Fold: BLIP-like Superfamily: BT0923-like Family: BT0923-like Phyre2 PDB 2fki chain A domain 1 Region: 40 - 114 Aligned: 69 Modelled: 75 Confidence: 32.8% Identity: 22% Fold: Secretion chaperone-like Superfamily: YjbR-like Family: YjbR-like Phyre2 PDB 3nrl chain B Region: 36 - 59 Aligned: 24 Modelled: 24 Confidence: 22.2% Identity: 25% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:uncharacterized protein rumgna_01417; PDBTitle: crystal structure of protein rumgna_01417 from ruminococcus gnavus,2 northeast structural genomics consortium target ugr76 Phyre2 PDB 1axi chain B domain 1 Region: 47 - 84 Aligned: 29 Modelled: 38 Confidence: 18.0% Identity: 28% Fold: Immunoglobulin-like beta-sandwich Superfamily: Fibronectin type III Family: Fibronectin type III Phyre2 PDB 3elg chain A domain 1 Region: 56 - 102 Aligned: 47 Modelled: 44 Confidence: 17.7% Identity: 15% Fold: BLIP-like Superfamily: BT0923-like Family: BT0923-like Phyre2 PDB 1agq chain B Region: 54 - 74 Aligned: 16 Modelled: 21 Confidence: 16.4% Identity: 31% PDB header:growth factor Chain: B: PDB Molecule:glial cell-derived neurotrophic factor; PDBTitle: glial cell-derived neurotrophic factor from rat Phyre2 PDB 1b63 chain A domain 1 Region: 34 - 52 Aligned: 19 Modelled: 19 Confidence: 16.0% Identity: 21% Fold: Ribosomal protein S5 domain 2-like Superfamily: Ribosomal protein S5 domain 2-like Family: DNA gyrase/MutL, second domain Phyre2 PDB 2p02 chain A domain 2 Region: 1 - 24 Aligned: 23 Modelled: 24 Confidence: 15.1% Identity: 35% Fold: S-adenosylmethionine synthetase Superfamily: S-adenosylmethionine synthetase Family: S-adenosylmethionine synthetase Phyre2 PDB 1mxa chain A domain 2 Region: 1 - 24 Aligned: 23 Modelled: 24 Confidence: 14.3% Identity: 30% Fold: S-adenosylmethionine synthetase Superfamily: S-adenosylmethionine synthetase Family: S-adenosylmethionine synthetase Phyre2 PDB 1qm4 chain A domain 2 Region: 1 - 24 Aligned: 23 Modelled: 24 Confidence: 13.0% Identity: 30% Fold: S-adenosylmethionine synthetase Superfamily: S-adenosylmethionine synthetase Family: S-adenosylmethionine synthetase Phyre2 PDB 2gyr chain B Region: 54 - 74 Aligned: 16 Modelled: 21 Confidence: 11.9% Identity: 13% PDB header:hormone/growth factor Chain: B: PDB Molecule:neurotrophic factor artemin, isoform 3; PDBTitle: crystal structure of human artemin Phyre2 PDB 1zym chain A domain 1 Region: 10 - 25 Aligned: 16 Modelled: 16 Confidence: 10.4% Identity: 19% Fold: SAM domain-like Superfamily: Enzyme I of the PEP:sugar phosphotransferase system HPr-binding (sub)domain Family: Enzyme I of the PEP:sugar phosphotransferase system HPr-binding (sub)domain Phyre2 PDB 2w82 chain C Region: 68 - 96 Aligned: 29 Modelled: 29 Confidence: 9.7% Identity: 28% PDB header:replication inhibitor Chain: C: PDB Molecule:orf18; PDBTitle: the structure of arda Phyre2 PDB 1lu0 chain A Region: 36 - 42 Aligned: 7 Modelled: 7 Confidence: 9.6% Identity: 86% PDB header:hydrolase inhibitor Chain: A: PDB Molecule:trypsin inhibitor i; PDBTitle: atomic resolution structure of squash trypsin inhibitor: unexpected2 metal coordination Phyre2 PDB 1lu0 chain A Region: 36 - 42 Aligned: 7 Modelled: 7 Confidence: 9.6% Identity: 86% Fold: Knottins (small inhibitors, toxins, lectins) Superfamily: Plant inhibitors of proteinases and amylases Family: Plant inhibitors of proteinases and amylases Phyre2 PDB 3cti chain A Region: 36 - 42 Aligned: 7 Modelled: 7 Confidence: 9.6% Identity: 86% PDB header:proteinase inhibitor (trypsin) Chain: A: PDB Molecule:trypsin inhibitor; PDBTitle: relaxation matrix refinement of the solution structure of2 squash trypsin inhibitor Phyre2 1 c3rpjA_ 2 d2bhua1 3 c2b9bA_ 4 d2a1va1 5 d3duea1 6 d2fkia1 7 c3nrlB_ 8 d1axib1 9 d3elga1 10 c1agqB_ 11 d1b63a1 12 d2p02a2 13 d1mxaa2 14 d1qm4a2 15 c2gyrB_ 16 d1zyma1 17 c2w82C_ 18 c1lu0A_ 19 d1lu0a_ 20 c3ctiA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c3rpjA_ 100.0 48 PDB header:transcription regulator Chain: A: PDB Molecule:curlin genes transcriptional regulator; PDBTitle: structure of a curlin genes transcriptional regulator protein from2 proteus mirabilis hi4320. 2 d2bhua1 67.4 20 Fold:Immunoglobulin-like beta-sandwich Superfamily:E set domains Family:E-set domains of sugar-utilizing enzymes 3 c2b9bA_ 62.2 44 PDB header:viral protein Chain: A: PDB Molecule:fusion glycoprotein f0; PDBTitle: structure of the parainfluenza virus 5 f protein in its metastable,2 pre-fusion conformation 4 d2a1va1 49.9 18 Fold:Secretion chaperone-like Superfamily:YjbR-like Family:YjbR-like 5 d3duea1 45.9 23 Fold:BLIP-like Superfamily:BT0923-like Family:BT0923-like 6 d2fkia1 32.8 22 Fold:Secretion chaperone-like Superfamily:YjbR-like Family:YjbR-like 7 c3nrlB_ 22.2 25 PDB header:structural genomics, unknown function Chain: B: PDB Molecule:uncharacterized protein rumgna_01417; PDBTitle: crystal structure of protein rumgna_01417 from ruminococcus gnavus,2 northeast structural genomics consortium target ugr76 8 d1axib1 18.0 28 Fold:Immunoglobulin-like beta-sandwich Superfamily:Fibronectin type III Family:Fibronectin type III 9 d3elga1 17.7 15 Fold:BLIP-like Superfamily:BT0923-like Family:BT0923-like 10 c1agqB_ 16.4 31 PDB header:growth factor Chain: B: PDB Molecule:glial cell-derived neurotrophic factor; PDBTitle: glial cell-derived neurotrophic factor from rat 11 d1b63a1 16.0 21 Fold:Ribosomal protein S5 domain 2-like Superfamily:Ribosomal protein S5 domain 2-like Family:DNA gyrase/MutL, second domain 12 d2p02a2 15.1 35 Fold:S-adenosylmethionine synthetase Superfamily:S-adenosylmethionine synthetase Family:S-adenosylmethionine synthetase 13 d1mxaa2 14.3 30 Fold:S-adenosylmethionine synthetase Superfamily:S-adenosylmethionine synthetase Family:S-adenosylmethionine synthetase 14 d1qm4a2 13.0 30 Fold:S-adenosylmethionine synthetase Superfamily:S-adenosylmethionine synthetase Family:S-adenosylmethionine synthetase 15 c2gyrB_ 11.9 13 PDB header:hormone/growth factor Chain: B: PDB Molecule:neurotrophic factor artemin, isoform 3; PDBTitle: crystal structure of human artemin 16 d1zyma1 10.4 19 Fold:SAM domain-like Superfamily:Enzyme I of the PEP:sugar phosphotransferase system HPr-binding (sub)domain Family:Enzyme I of the PEP:sugar phosphotransferase system HPr-binding (sub)domain 17 c2w82C_ 9.7 28 PDB header:replication inhibitor Chain: C: PDB Molecule:orf18; PDBTitle: the structure of arda 18 c1lu0A_ 9.6 86 PDB header:hydrolase inhibitor Chain: A: PDB Molecule:trypsin inhibitor i; PDBTitle: atomic resolution structure of squash trypsin inhibitor: unexpected2 metal coordination 19 d1lu0a_ 9.6 86 Fold:Knottins (small inhibitors, toxins, lectins) Superfamily:Plant inhibitors of proteinases and amylases Family:Plant inhibitors of proteinases and amylases 20 c3ctiA_ 9.6 86 PDB header:proteinase inhibitor (trypsin) Chain: A: PDB Molecule:trypsin inhibitor; PDBTitle: relaxation matrix refinement of the solution structure of2 squash trypsin inhibitor 21 c2ctiA_ not modelled 9.6 86 PDB header:proteinase inhibitor (trypsin) Chain: A: PDB Molecule:trypsin inhibitor; PDBTitle: determination of the complete three-dimensional structure2 of the trypsin inhibitor from squash seeds in aqueous3 solution by nuclear magnetic resonance and a combination4 of distance geometry and dynamical simulated annealing 22 c1ppeI_ not modelled 9.6 86 PDB header:hydrolase(serine proteinase) Chain: I: PDB Molecule:trypsin inhibitor cmti-i; PDBTitle: the refined 2.0 angstroms x-ray crystal structure of the2 complex formed between bovine beta-trypsin and cmti-i, a3 trypsin inhibitor from squash seeds (cucurbita maxima):4 topological similarity of the squash seed inhibitors with5 the carboxypeptidase a inhibitor from potatoes 23 c1ctiA_ not modelled 9.6 86 PDB header:proteinase inhibitor (trypsin) Chain: A: PDB Molecule:trypsin inhibitor; PDBTitle: determination of the complete three-dimensional structure2 of the trypsin inhibitor from squash seeds in aqueous3 solution by nuclear magnetic resonance and a combination4 of distance geometry and dynamical simulated annealing 24 c2staI_ not modelled 9.6 86 PDB header:hydrolase/hydrolase inhibitor Chain: I: PDB Molecule:protein (trypsin inhibitor); PDBTitle: anionic salmon trypsin in complex with squash seed2 inhibitor (cucurbita maxima trypsin inhibitor i) 25 d2id1a1 not modelled 9.5 25 Fold:Nucleotidyltransferase Superfamily:Nucleotidyltransferase Family:Iojap/YbeB-like 26 c1lu0B_ not modelled 9.5 86 PDB header:hydrolase inhibitor Chain: B: PDB Molecule:trypsin inhibitor i; PDBTitle: atomic resolution structure of squash trypsin inhibitor: unexpected2 metal coordination 27 c2v1vA_ not modelled 9.5 86 PDB header:hydrolase inhibitor Chain: A: PDB Molecule:trypsin inhibitor 1; PDBTitle: 3d structure of the m8l mutant of squash trypsin inhibitor2 cmti-i 28 c2a7oA_ not modelled 9.2 50 PDB header:transcription Chain: A: PDB Molecule:huntingtin interacting protein b; PDBTitle: solution structure of the hset2/hypb sri domain 29 d2c4ba2 not modelled 9.2 86 Fold:Knottins (small inhibitors, toxins, lectins) Superfamily:Plant inhibitors of proteinases and amylases Family:Plant inhibitors of proteinases and amylases 30 c2vpzG_ not modelled 9.2 50 PDB header:oxidoreductase Chain: G: PDB Molecule:hypothetical membrane spanning protein; PDBTitle: polysulfide reductase native structure 31 d1h9ii_ not modelled 9.1 60 Fold:Knottins (small inhibitors, toxins, lectins) Superfamily:Plant inhibitors of proteinases and amylases Family:Plant inhibitors of proteinases and amylases 32 d1h9hi_ not modelled 8.8 86 Fold:Knottins (small inhibitors, toxins, lectins) Superfamily:Plant inhibitors of proteinases and amylases Family:Plant inhibitors of proteinases and amylases 33 c1mcvI_ not modelled 8.8 86 PDB header:hydrolase Chain: I: PDB Molecule:hei-toe i; PDBTitle: crystal structure analysis of a hybrid squash inhibitor in2 complex with porcine pancreatic elastase 34 d1mcvi_ not modelled 8.8 86 Fold:Knottins (small inhibitors, toxins, lectins) Superfamily:Plant inhibitors of proteinases and amylases Family:Plant inhibitors of proteinases and amylases 35 c2it7A_ not modelled 8.7 86 PDB header:plant protein Chain: A: PDB Molecule:trypsin inhibitor 2; PDBTitle: solution structure of the squash trypsin inhibitor eeti-ii 36 d2it7a1 not modelled 8.7 86 Fold:Knottins (small inhibitors, toxins, lectins) Superfamily:Plant inhibitors of proteinases and amylases Family:Plant inhibitors of proteinases and amylases 37 c2letA_ not modelled 8.5 86 PDB header:proteinase inhibitor(trypsin) Chain: A: PDB Molecule:trypsin inhibitor ii; PDBTitle: an 1h nmr determination of the three dimensional structures2 of mirror image forms of a leu-5 variant of the trypsin3 inhibitor ecballium elaterium (eeti-ii) 38 d1rpya_ not modelled 8.1 19 Fold:SH2-like Superfamily:SH2 domain Family:SH2 domain 39 c3m07A_ not modelled 8.0 11 PDB header:unknown function Chain: A: PDB Molecule:putative alpha amylase; PDBTitle: 1.4 angstrom resolution crystal structure of putative alpha2 amylase from salmonella typhimurium. 40 c3fg7A_ not modelled 7.6 16 PDB header:structural protein Chain: A: PDB Molecule:villin-1; PDBTitle: the crystal structure of villin domain 6 41 d1xa6a2 not modelled 7.6 18 Fold:SH2-like Superfamily:SH2 domain Family:SH2 domain 42 d2evra2 not modelled 7.3 26 Fold:Cysteine proteinases Superfamily:Cysteine proteinases Family:NlpC/P60 43 c1ik9C_ not modelled 7.3 38 PDB header:gene regulation/ligase Chain: C: PDB Molecule:dna ligase iv; PDBTitle: crystal structure of a xrcc4-dna ligase iv complex 44 d2o62a1 not modelled 7.2 23 Fold:Lipocalins Superfamily:Lipocalins Family:All1756-like 45 d2i1sa1 not modelled 6.9 27 Fold:MM3350-like Superfamily:MM3350-like Family:MM3350-like 46 d1exbe_ not modelled 6.6 21 Fold:POZ domain Superfamily:POZ domain Family:Tetramerization domain of potassium channels 47 d2btci_ not modelled 6.5 71 Fold:Knottins (small inhibitors, toxins, lectins) Superfamily:Plant inhibitors of proteinases and amylases Family:Plant inhibitors of proteinases and amylases 48 c2btcI_ not modelled 6.5 71 PDB header:hydrolase/hydrolase inhibitor Chain: I: PDB Molecule:protein (trypsin inhibitor); PDBTitle: bovine trypsin in complex with squash seed inhibitor2 (cucurbita pepo trypsin inhibitor ii) 49 c2stbI_ not modelled 6.5 71 PDB header:hydrolase/hydrolase inhibitor Chain: I: PDB Molecule:protein (trypsin inhibitor); PDBTitle: anionic salmon trypsin in complex with squash seed2 inhibitor (cucurbita pepo trypsin inhibitor ii) 50 c3h9xB_ not modelled 6.3 13 PDB header:structural genomics, unknown function Chain: B: PDB Molecule:uncharacterized protein pspto_3016; PDBTitle: crystal structure of the pspto_3016 protein from2 pseudomonas syringae, northeast structural genomics3 consortium target psr293 51 c2ysxA_ not modelled 6.2 15 PDB header:signaling protein Chain: A: PDB Molecule:signaling inositol polyphosphate phosphatase PDBTitle: solution structure of the human ship sh2 domain 52 c2yh5A_ not modelled 6.1 12 PDB header:lipid binding protein Chain: A: PDB Molecule:dapx protein; PDBTitle: structure of the c-terminal domain of bamc 53 c2xznZ_ not modelled 6.1 11 PDB header:ribosome Chain: Z: PDB Molecule:rps21e; PDBTitle: crystal structure of the eukaryotic 40s ribosomal2 subunit in complex with initiation factor 1. this file3 contains the 40s subunit and initiation factor for4 molecule 2 54 d1t6aa_ not modelled 6.0 12 Fold:TBP-like Superfamily:Rbstp2229 protein Family:Rbstp2229 protein 55 d2eyva1 not modelled 6.0 19 Fold:SH2-like Superfamily:SH2 domain Family:SH2 domain 56 d1eysl_ not modelled 5.9 67 Fold:Bacterial photosystem II reaction centre, L and M subunits Superfamily:Bacterial photosystem II reaction centre, L and M subunits Family:Bacterial photosystem II reaction centre, L and M subunits 57 c2kkuA_ not modelled 5.8 38 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein; PDBTitle: solution structure of protein af2351 from archaeoglobus2 fulgidus. northeast structural genomics consortium target3 att9/ontario center for structural proteomics target af2351 58 d2bcqa3 not modelled 5.5 25 Fold:Nucleotidyltransferase Superfamily:Nucleotidyltransferase Family:DNA polymerase beta-like 59 c2nvgA_ not modelled 5.5 13 PDB header:oxidoreductase Chain: A: PDB Molecule:ubiquinol-cytochrome c reductase iron-sulfur subunit; PDBTitle: soluble domain of rieske iron sulfur protein. 60 c2qv5A_ not modelled 5.5 23 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein atu2773; PDBTitle: crystal structure of uncharacterized protein atu2773 from2 agrobacterium tumefaciens c58 61 c3rv2B_ not modelled 5.4 43 PDB header:transferase Chain: B: PDB Molecule:s-adenosylmethionine synthase; PDBTitle: crystal structure of s-adenosylmethionine synthetase from2 mycobacterium marinum

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0