Secondary structure and disorder prediction

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10

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20

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40

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50

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60

Sequence

M

L

N

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C

R

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P

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T

D

R

K

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K

E

K

R

A

M

K

Q

H

K

A

M

I

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A

L

I

V

I

C

I

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A

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V

A

A

L

V

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R

K

D

L

C

E

V

H

I

R

T

G

Q

T

E

V

Secondary structure

SS confidence

Disorder

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Disorder confidence

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Sequence

A

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F

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A

Y

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S

E

Secondary structure

SS confidence

Disorder

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Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1y4o chain A domain 1 Region: 18 - 65 Aligned: 48 Modelled: 48 Confidence: 27.0% Identity: 17% Fold: Profilin-like Superfamily: Roadblock/LC7 domain Family: Roadblock/LC7 domain Phyre2 PDB 2hz5 chain A domain 1 Region: 18 - 62 Aligned: 45 Modelled: 45 Confidence: 25.7% Identity: 18% Fold: Profilin-like Superfamily: Roadblock/LC7 domain Family: Roadblock/LC7 domain Phyre2 PDB 1prt chain B domain 1 Region: 44 - 60 Aligned: 17 Modelled: 17 Confidence: 11.8% Identity: 41% Fold: OB-fold Superfamily: Bacterial enterotoxins Family: Bacterial AB5 toxins, B-subunits Phyre2 PDB 1prt chain C domain 1 Region: 44 - 60 Aligned: 17 Modelled: 17 Confidence: 9.2% Identity: 41% Fold: OB-fold Superfamily: Bacterial enterotoxins Family: Bacterial AB5 toxins, B-subunits Phyre2 PDB 1bcp chain H Region: 44 - 60 Aligned: 17 Modelled: 17 Confidence: 7.1% Identity: 41% PDB header:toxin Chain: H: PDB Molecule:pertussis toxin; PDBTitle: binary complex of pertussis toxin and atp Phyre2 PDB 1u7m chain B Region: 16 - 24 Aligned: 9 Modelled: 9 Confidence: 7.1% Identity: 44% PDB header:de novo protein Chain: B: PDB Molecule:four-helix bundle model; PDBTitle: solution structure of a diiron protein model: due ferri(ii)2 turn mutant Phyre2 PDB 2jug chain B Region: 5 - 31 Aligned: 27 Modelled: 27 Confidence: 6.9% Identity: 11% PDB header:biosynthetic protein Chain: B: PDB Molecule:tubc protein; PDBTitle: multienzyme docking in hybrid megasynthetases Phyre2 PDB 2rpi chain A Region: 35 - 55 Aligned: 21 Modelled: 21 Confidence: 6.6% Identity: 29% PDB header:hydrolase Chain: A: PDB Molecule:ribonuclease h; PDBTitle: the nmr structure of the submillisecond folding2 intermediate of the thermus thermophilus ribonuclease h Phyre2 PDB 1zll chain E Region: 6 - 38 Aligned: 33 Modelled: 33 Confidence: 5.9% Identity: 24% PDB header:membrane protein/signaling protein Chain: E: PDB Molecule:cardiac phospholamban; PDBTitle: nmr structure of unphosphorylated human phospholamban2 pentamer Phyre2 PDB 1ehs chain A Region: 45 - 50 Aligned: 6 Modelled: 6 Confidence: 5.4% Identity: 83% Fold: Toxic hairpin Superfamily: Heat-stable enterotoxin B Family: Heat-stable enterotoxin B Phyre2 1 d1y4oa1 2 d2hz5a1 3 d1prtb1 4 d1prtc1 5 c1bcpH_ 6 c1u7mB_ 7 c2jugB_ 8 c2rpiA_ 9 c1zllE_ 10 d1ehsa_

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1y4oa1 27.0 17 Fold:Profilin-like Superfamily:Roadblock/LC7 domain Family:Roadblock/LC7 domain 2 d2hz5a1 25.7 18 Fold:Profilin-like Superfamily:Roadblock/LC7 domain Family:Roadblock/LC7 domain 3 d1prtb1 11.8 41 Fold:OB-fold Superfamily:Bacterial enterotoxins Family:Bacterial AB5 toxins, B-subunits 4 d1prtc1 9.2 41 Fold:OB-fold Superfamily:Bacterial enterotoxins Family:Bacterial AB5 toxins, B-subunits 5 c1bcpH_ 7.1 41 PDB header:toxin Chain: H: PDB Molecule:pertussis toxin; PDBTitle: binary complex of pertussis toxin and atp 6 c1u7mB_ 7.1 44 PDB header:de novo protein Chain: B: PDB Molecule:four-helix bundle model; PDBTitle: solution structure of a diiron protein model: due ferri(ii)2 turn mutant 7 c2jugB_ 6.9 11 PDB header:biosynthetic protein Chain: B: PDB Molecule:tubc protein; PDBTitle: multienzyme docking in hybrid megasynthetases 8 c2rpiA_ 6.6 29 PDB header:hydrolase Chain: A: PDB Molecule:ribonuclease h; PDBTitle: the nmr structure of the submillisecond folding2 intermediate of the thermus thermophilus ribonuclease h 9 c1zllE_ 5.9 24 PDB header:membrane protein/signaling protein Chain: E: PDB Molecule:cardiac phospholamban; PDBTitle: nmr structure of unphosphorylated human phospholamban2 pentamer 10 d1ehsa_ 5.4 83 Fold:Toxic hairpin Superfamily:Heat-stable enterotoxin B Family:Heat-stable enterotoxin B

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0