Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	N	Y	R	Y	R	A	M	T	Q	D	G	Q	K	L	Q	G	I	I	D	A	N	D	E	R	Q	A	R	L	R	L	R	E	E	G	L	F	L	L	D	I	R	P	Q	K	S	S	G	V	K	T	R	R	P	R	I	S	H	S	E
Secondary structure
SS confidence
Disorder		?	?								?																								?									?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		L	T	L	F	T	R	Q	L	A	T	L	S	A	A	A	L	P	L	E	E	S	L	A	V	I	G	Q	Q	S	S	N	K	R	L	G	D	V	L	N	Q	V	R	S	A	I	L	E	G	H	P	L	S	D	A	L	Q	H	F	P	T
Secondary structure
SS confidence
Disorder																												?	?																			?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		L	F	D	S	L	Y	R	T	L	V	K	A	G	E	K	S	G	L	L	A	P	V	L	E	K	L	A	D	Y	N	E	N	R	Q	K	I	R	S	K	L	I	Q	S	L	I	Y	P	C	M	L	T	T	V	A	I	G	V	V	I	I
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		L	L	T	A	V	V	P	K	I	T	E	Q	F	V	H	M	K	Q	Q	L	P	L	S	T	R	I	L	L	G	L	S	D	T	L	Q	R	T	G	P	T	L	L	A	T	V	F	I	V	A	V	G	F	W	L	W	L	K	R	G	N
Secondary structure
SS confidence
Disorder																		?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		N	R	H	R	F	H	A	M	L	L	R	V	A	L	I	G	P	L	I	C	A	I	N	S	A	R	Y	L	R	T	L	S	I	L	Q	S	S	G	V	P	L	L	D	G	M	N	L	S	T	E	S	L	N	N	L	E	I	R	Q	R
Secondary structure
SS confidence
Disorder																																																						?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		L	A	N	A	A	E	N	V	R	Q	G	N	S	I	H	L	S	L	E	Q	T	A	I	F	P	P	M	M	L	Y	M	V	A	S	G	E	K	S	G	Q	L	G	T	L	M	V	R	A	A	D	N	Q	E	T	L	Q	Q	N	R	I
Secondary structure
SS confidence
Disorder											?	?	?	?																								?		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.
Sequence		A	L	T	L	S	I	F	E	P	A	L	I	I	T	M	A	L	I	V	L	F	I	V	V	S	V	L	Q	P	L	L	Q	L	N	S	M	I	N
Secondary structure
SS confidence
Disorder																																				?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 3c1q chain A Region: 50 - 163 Aligned: 114 Modelled: 114 Confidence: 99.9% Identity: 51% PDB header:transport protein Chain: A: PDB Molecule:general secretion pathway protein f; PDBTitle: the three-dimensional structure of the cytoplasmic domains of epsf2 from the type 2 secretion system of vibrio cholerae Phyre2 PDB 2whn chain A Region: 250 - 359 Aligned: 110 Modelled: 110 Confidence: 99.9% Identity: 23% PDB header:protein transport Chain: A: PDB Molecule:pilus assembly protein pilc; PDBTitle: n-terminal domain from the pilc type iv pilus biogenesis2 protein Phyre2 PDB 3c8m chain A Region: 74 - 117 Aligned: 44 Modelled: 44 Confidence: 42.1% Identity: 18% PDB header:oxidoreductase Chain: A: PDB Molecule:homoserine dehydrogenase; PDBTitle: crystal structure of homoserine dehydrogenase from thermoplasma2 volcanium Phyre2 PDB 3cum chain A domain 1 Region: 254 - 355 Aligned: 102 Modelled: 102 Confidence: 38.1% Identity: 8% Fold: 6-phosphogluconate dehydrogenase C-terminal domain-like Superfamily: 6-phosphogluconate dehydrogenase C-terminal domain-like Family: Hydroxyisobutyrate and 6-phosphogluconate dehydrogenase domain Phyre2 PDB 3i3a chain C Region: 42 - 103 Aligned: 62 Modelled: 62 Confidence: 36.5% Identity: 8% PDB header:transferase Chain: C: PDB Molecule:acyl-[acyl-carrier-protein]--udp-n- PDBTitle: structural basis for the sugar nucleotide and acyl chain2 selectivity of leptospira interrogans lpxa Phyre2 PDB 3ing chain A Region: 74 - 117 Aligned: 44 Modelled: 44 Confidence: 34.4% Identity: 27% PDB header:oxidoreductase Chain: A: PDB Molecule:homoserine dehydrogenase; PDBTitle: crystal structure of homoserine dehydrogenase (np_394635.1) from2 thermoplasma acidophilum at 1.95 a resolution Phyre2 PDB 2ejw chain B Region: 74 - 117 Aligned: 44 Modelled: 44 Confidence: 34.2% Identity: 16% PDB header:oxidoreductase Chain: B: PDB Molecule:homoserine dehydrogenase; PDBTitle: homoserine dehydrogenase from thermus thermophilus hb8 Phyre2 PDB 2d1l chain A Region: 80 - 164 Aligned: 84 Modelled: 85 Confidence: 33.4% Identity: 10% PDB header:protein binding Chain: A: PDB Molecule:metastasis suppressor protein 1; PDBTitle: structure of f-actin binding domain imd of mim (missing in metastasis) Phyre2 PDB 3ok8 chain A Region: 91 - 164 Aligned: 73 Modelled: 74 Confidence: 31.0% Identity: 15% PDB header:protein binding Chain: A: PDB Molecule:brain-specific angiogenesis inhibitor 1-associated protein PDBTitle: i-bar of pinkbar Phyre2 PDB 1y2o chain A domain 1 Region: 78 - 164 Aligned: 85 Modelled: 83 Confidence: 29.7% Identity: 15% Fold: BAR/IMD domain-like Superfamily: BAR/IMD domain-like Family: IMD domain Phyre2 PDB 3iug chain A Region: 108 - 160 Aligned: 52 Modelled: 53 Confidence: 20.0% Identity: 17% PDB header:splicing Chain: A: PDB Molecule:rho/cdc42/rac gtpase-activating protein rics; PDBTitle: crystal structure of the rhogap domain of rics Phyre2 PDB 2jf2 chain A domain 1 Region: 42 - 103 Aligned: 62 Modelled: 62 Confidence: 17.0% Identity: 11% Fold: Single-stranded left-handed beta-helix Superfamily: Trimeric LpxA-like enzymes Family: UDP N-acetylglucosamine acyltransferase Phyre2 PDB 3k4i chain C Region: 99 - 118 Aligned: 20 Modelled: 20 Confidence: 15.1% Identity: 25% PDB header:structural genomics, unknown function Chain: C: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of uncharacterized protein pspto_3204 from2 pseudomonas syringae pv. tomato str. dc3000 Phyre2 PDB 3r1f chain O Region: 111 - 150 Aligned: 40 Modelled: 40 Confidence: 14.5% Identity: 15% PDB header:transcription Chain: O: PDB Molecule:esx-1 secretion-associated regulator espr; PDBTitle: crystal structure of a key regulator of virulence in mycobacterium2 tuberculosis Phyre2 PDB 2c5q chain E Region: 92 - 122 Aligned: 31 Modelled: 31 Confidence: 14.1% Identity: 19% PDB header:structural genomics,unknown function Chain: E: PDB Molecule:rraa-like protein yer010c; PDBTitle: crystal structure of yeast yer010cp Phyre2 PDB 2bbj chain B Region: 59 - 240 Aligned: 134 Modelled: 147 Confidence: 13.0% Identity: 17% PDB header:metal transport/membrane protein Chain: B: PDB Molecule:divalent cation transport-related protein; PDBTitle: crystal structure of the cora mg2+ transporter Phyre2 PDB 1xa6 chain A domain 1 Region: 108 - 160 Aligned: 53 Modelled: 53 Confidence: 12.8% Identity: 13% Fold: GTPase activation domain, GAP Superfamily: GTPase activation domain, GAP Family: BCR-homology GTPase activation domain (BH-domain) Phyre2 PDB 3kuq chain A Region: 108 - 160 Aligned: 53 Modelled: 53 Confidence: 12.8% Identity: 15% PDB header:hydrolase activator Chain: A: PDB Molecule:rho gtpase-activating protein 7; PDBTitle: crystal structure of the dlc1 rhogap domain Phyre2 PDB 3byi chain A Region: 108 - 160 Aligned: 53 Modelled: 53 Confidence: 12.7% Identity: 19% PDB header:signaling protein Chain: A: PDB Molecule:rho gtpase activating protein 15; PDBTitle: crystal structure of human rho gtpase activating protein 15 (arhgap15) Phyre2 PDB 1vq3 chain A Region: 10 - 33 Aligned: 24 Modelled: 24 Confidence: 11.9% Identity: 13% Fold: PurS-like Superfamily: PurS-like Family: PurS subunit of FGAM synthetase Phyre2 1 c3c1qA_ 2 c2whnA_ 3 c3c8mA_ 4 d3cuma1 5 c3i3aC_ 6 c3ingA_ 7 c2ejwB_ 8 c2d1lA_ 9 c3ok8A_ 10 d1y2oa1 11 c3iugA_ 12 d2jf2a1 13 c3k4iC_ 14 c3r1fO_ 15 c2c5qE_ 16 c2bbjB_ 17 d1xa6a1 18 c3kuqA_ 19 c3byiA_ 20 d1vq3a_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c3c1qA_ 99.9 51 PDB header:transport protein Chain: A: PDB Molecule:general secretion pathway protein f; PDBTitle: the three-dimensional structure of the cytoplasmic domains of epsf2 from the type 2 secretion system of vibrio cholerae 2 c2whnA_ 99.9 23 PDB header:protein transport Chain: A: PDB Molecule:pilus assembly protein pilc; PDBTitle: n-terminal domain from the pilc type iv pilus biogenesis2 protein 3 c3c8mA_ 42.1 18 PDB header:oxidoreductase Chain: A: PDB Molecule:homoserine dehydrogenase; PDBTitle: crystal structure of homoserine dehydrogenase from thermoplasma2 volcanium 4 d3cuma1 38.1 8 Fold:6-phosphogluconate dehydrogenase C-terminal domain-like Superfamily:6-phosphogluconate dehydrogenase C-terminal domain-like Family:Hydroxyisobutyrate and 6-phosphogluconate dehydrogenase domain 5 c3i3aC_ 36.5 8 PDB header:transferase Chain: C: PDB Molecule:acyl-[acyl-carrier-protein]--udp-n- PDBTitle: structural basis for the sugar nucleotide and acyl chain2 selectivity of leptospira interrogans lpxa 6 c3ingA_ 34.4 27 PDB header:oxidoreductase Chain: A: PDB Molecule:homoserine dehydrogenase; PDBTitle: crystal structure of homoserine dehydrogenase (np_394635.1) from2 thermoplasma acidophilum at 1.95 a resolution 7 c2ejwB_ 34.2 16 PDB header:oxidoreductase Chain: B: PDB Molecule:homoserine dehydrogenase; PDBTitle: homoserine dehydrogenase from thermus thermophilus hb8 8 c2d1lA_ 33.4 10 PDB header:protein binding Chain: A: PDB Molecule:metastasis suppressor protein 1; PDBTitle: structure of f-actin binding domain imd of mim (missing in metastasis) 9 c3ok8A_ 31.0 15 PDB header:protein binding Chain: A: PDB Molecule:brain-specific angiogenesis inhibitor 1-associated protein PDBTitle: i-bar of pinkbar 10 d1y2oa1 29.7 15 Fold:BAR/IMD domain-like Superfamily:BAR/IMD domain-like Family:IMD domain 11 c3iugA_ 20.0 17 PDB header:splicing Chain: A: PDB Molecule:rho/cdc42/rac gtpase-activating protein rics; PDBTitle: crystal structure of the rhogap domain of rics 12 d2jf2a1 17.0 11 Fold:Single-stranded left-handed beta-helix Superfamily:Trimeric LpxA-like enzymes Family:UDP N-acetylglucosamine acyltransferase 13 c3k4iC_ 15.1 25 PDB header:structural genomics, unknown function Chain: C: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of uncharacterized protein pspto_3204 from2 pseudomonas syringae pv. tomato str. dc3000 14 c3r1fO_ 14.5 15 PDB header:transcription Chain: O: PDB Molecule:esx-1 secretion-associated regulator espr; PDBTitle: crystal structure of a key regulator of virulence in mycobacterium2 tuberculosis 15 c2c5qE_ 14.1 19 PDB header:structural genomics,unknown function Chain: E: PDB Molecule:rraa-like protein yer010c; PDBTitle: crystal structure of yeast yer010cp 16 c2bbjB_ 13.0 17 PDB header:metal transport/membrane protein Chain: B: PDB Molecule:divalent cation transport-related protein; PDBTitle: crystal structure of the cora mg2+ transporter 17 d1xa6a1 12.8 13 Fold:GTPase activation domain, GAP Superfamily:GTPase activation domain, GAP Family:BCR-homology GTPase activation domain (BH-domain) 18 c3kuqA_ 12.8 15 PDB header:hydrolase activator Chain: A: PDB Molecule:rho gtpase-activating protein 7; PDBTitle: crystal structure of the dlc1 rhogap domain 19 c3byiA_ 12.7 19 PDB header:signaling protein Chain: A: PDB Molecule:rho gtpase activating protein 15; PDBTitle: crystal structure of human rho gtpase activating protein 15 (arhgap15) 20 d1vq3a_ 11.9 13 Fold:PurS-like Superfamily:PurS-like Family:PurS subunit of FGAM synthetase 21 d2ezla_ not modelled 11.4 14 Fold:DNA/RNA-binding 3-helical bundle Superfamily:Homeodomain-like Family:Recombinase DNA-binding domain 22 c2f4qA_ not modelled 10.7 18 PDB header:isomerase Chain: A: PDB Molecule:type i topoisomerase, putative; PDBTitle: crystal structure of deinococcus radiodurans topoisomerase ib 23 c3ibqA_ not modelled 10.7 22 PDB header:transferase Chain: A: PDB Molecule:pyridoxal kinase; PDBTitle: crystal structure of pyridoxal kinase from lactobacillus2 plantarum in complex with atp 24 c3r0sA_ not modelled 10.5 16 PDB header:transferase Chain: A: PDB Molecule:acyl-[acyl-carrier-protein]--udp-n-acetylglucosamine o- PDBTitle: udp-n-acetylglucosamine acyltransferase from campylobacter jejuni 25 c3do5A_ not modelled 10.5 18 PDB header:oxidoreductase Chain: A: PDB Molecule:homoserine dehydrogenase; PDBTitle: crystal structure of putative homoserine dehydrogenase (np_069768.1)2 from archaeoglobus fulgidus at 2.20 a resolution 26 c2hx1D_ not modelled 10.4 11 PDB header:hydrolase Chain: D: PDB Molecule:predicted sugar phosphatases of the had PDBTitle: crystal structure of possible sugar phosphatase, had2 superfamily (zp_00311070.1) from cytophaga hutchinsonii3 atcc 33406 at 2.10 a resolution 27 d1j2za_ not modelled 9.3 12 Fold:Single-stranded left-handed beta-helix Superfamily:Trimeric LpxA-like enzymes Family:UDP N-acetylglucosamine acyltransferase 28 d1gtda_ not modelled 9.3 4 Fold:PurS-like Superfamily:PurS-like Family:PurS subunit of FGAM synthetase 29 c2zw2B_ not modelled 9.2 13 PDB header:ligase Chain: B: PDB Molecule:putative uncharacterized protein sts178; PDBTitle: crystal structure of formylglycinamide ribonucleotide amidotransferase2 iii from sulfolobus tokodaii (stpurs) 30 c1xa6A_ not modelled 8.9 13 PDB header:signaling protein Chain: A: PDB Molecule:beta2-chimaerin; PDBTitle: crystal structure of the human beta2-chimaerin 31 c3neuA_ not modelled 8.8 15 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:lin1836 protein; PDBTitle: the crystal structure of a functionally-unknown protein lin1836 from2 listeria innocua clip11262 32 c2pmzN_ not modelled 8.4 13 PDB header:translation, transferase Chain: N: PDB Molecule:dna-directed rna polymerase subunit n; PDBTitle: archaeal rna polymerase from sulfolobus solfataricus 33 d1hara_ not modelled 8.3 5 Fold:DNA/RNA polymerases Superfamily:DNA/RNA polymerases Family:Reverse transcriptase 34 d2f06a1 not modelled 7.8 19 Fold:Ferredoxin-like Superfamily:ACT-like Family:BT0572-like 35 d1e8ob_ not modelled 7.6 23 Fold:Signal recognition particle alu RNA binding heterodimer, SRP9/14 Superfamily:Signal recognition particle alu RNA binding heterodimer, SRP9/14 Family:Signal recognition particle alu RNA binding heterodimer, SRP9/14 36 c3mtjA_ not modelled 7.5 16 PDB header:oxidoreductase Chain: A: PDB Molecule:homoserine dehydrogenase; PDBTitle: the crystal structure of a homoserine dehydrogenase from thiobacillus2 denitrificans to 2.15a 37 d2c4na1 not modelled 6.8 14 Fold:HAD-like Superfamily:HAD-like Family:NagD-like 38 d2gyqa1 not modelled 6.3 18 Fold:Ferritin-like Superfamily:Ferritin-like Family:YciF-like 39 c3gdeA_ not modelled 5.7 10 PDB header:ligase Chain: A: PDB Molecule:dna ligase; PDBTitle: the closed conformation of atp-dependent dna ligase from2 archaeoglobus fulgidus 40 d1ijwc_ not modelled 5.7 27 Fold:DNA/RNA-binding 3-helical bundle Superfamily:Homeodomain-like Family:Recombinase DNA-binding domain 41 c1n54A_ not modelled 5.6 21 PDB header:rna binding protein Chain: A: PDB Molecule:80 kda nuclear cap binding protein; PDBTitle: cap binding complex m7gpppg free 42 c1zk6A_ not modelled 5.6 13 PDB header:isomerase Chain: A: PDB Molecule:foldase protein prsa; PDBTitle: nmr solution structure of b. subtilis prsa ppiase 43 d1jnsa_ not modelled 5.6 8 Fold:FKBP-like Superfamily:FKBP-like Family:FKBP immunophilin/proline isomerase 44 d1zhva2 not modelled 5.5 29 Fold:Ferredoxin-like Superfamily:ACT-like Family:Atu0741-like 45 c2i7aA_ not modelled 5.4 14 PDB header:hydrolase Chain: A: PDB Molecule:calpain 13; PDBTitle: domain iv of human calpain 13 46 c2kk6A_ not modelled 5.4 24 PDB header:transferase Chain: A: PDB Molecule:proto-oncogene tyrosine-protein kinase fer; PDBTitle: solution structure of sh2 domain of proto-oncogene tyrosine-2 protein kinase fer from homo sapiens, northeast structural3 genomics consortium (nesg) target hr3461d 47 d1914a2 not modelled 5.3 23 Fold:Signal recognition particle alu RNA binding heterodimer, SRP9/14 Superfamily:Signal recognition particle alu RNA binding heterodimer, SRP9/14 Family:Signal recognition particle alu RNA binding heterodimer, SRP9/14 48 d2gs4a1 not modelled 5.1 20 Fold:Ferritin-like Superfamily:Ferritin-like Family:YciF-like 49 c2kncB_ not modelled 5.1 10 PDB header:cell adhesion Chain: B: PDB Molecule:integrin beta-3; PDBTitle: platelet integrin alfaiib-beta3 transmembrane-cytoplasmic2 heterocomplex 50 c1ebuA_ not modelled 5.1 23 PDB header:oxidoreductase Chain: A: PDB Molecule:homoserine dehydrogenase; PDBTitle: homoserine dehydrogenase complex with nad analogue and l-2 homoserine 51 c2kncA_ not modelled 5.1 9 PDB header:cell adhesion Chain: A: PDB Molecule:integrin alpha-iib; PDBTitle: platelet integrin alfaiib-beta3 transmembrane-cytoplasmic2 heterocomplex 52 c1sneB_ not modelled 5.1 31 PDB header:de novo protein Chain: B: PDB Molecule:tetrameric beta-beta-alpha mini-protein; PDBTitle: an oligomeric domain-swapped beta-beta-alpha mini-protein 53 c1sneA_ not modelled 5.1 31 PDB header:de novo protein Chain: A: PDB Molecule:tetrameric beta-beta-alpha mini-protein; PDBTitle: an oligomeric domain-swapped beta-beta-alpha mini-protein 54 d1hcra_ not modelled 5.1 27 Fold:DNA/RNA-binding 3-helical bundle Superfamily:Homeodomain-like Family:Recombinase DNA-binding domain 55 c3nmeA_ not modelled 5.0 17 PDB header:hydrolase Chain: A: PDB Molecule:sex4 glucan phosphatase; PDBTitle: structure of a plant phosphatase

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0