Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	S	D	K	K	K	R	S	M	A	G	L	P	W	I	A	A	M	A	F	F	M	Q	A	L	D	A	T	I	L	N	T	A	L	P	A	I	A	H	S	L	N	R	S	P	L	A	M	Q	S	A	I	I	S	Y	T	L	T	V	A
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?																																	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		M	L	I	P	V	S	G	W	L	A	D	R	F	G	T	R	R	I	F	T	L	A	V	S	L	F	T	L	G	S	L	A	C	A	L	S	N	S	L	P	Q	L	V	V	F	R	V	I	Q	G	I	G	G	A	M	M	M	P	V	A
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		R	L	A	L	L	R	A	Y	P	R	N	E	L	L	P	V	L	N	F	V	A	M	P	G	L	V	G	P	I	L	G	P	V	L	G	G	V	L	V	T	W	A	T	W	H	W	I	F	L	I	N	I	P	I	G	I	A	G	L	L
Secondary structure
SS confidence
Disorder								?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		Y	A	R	K	H	M	P	N	F	T	T	A	R	R	R	F	D	I	T	G	F	L	L	F	G	L	S	L	V	L	F	S	S	G	I	E	L	F	G	E	K	I	V	A	S	W	I	A	L	T	V	I	V	T	S	I	G	L	L	L
Secondary structure
SS confidence
Disorder												?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		L	Y	I	L	H	A	R	R	T	P	N	P	L	I	S	L	D	L	F	K	T	R	T	F	S	I	G	I	V	G	N	I	A	T	R	L	G	T	G	C	V	P	F	L	M	P	L	M	L	Q	V	G	F	G	Y	Q	A	F	I	A
Secondary structure
SS confidence
Disorder														?							?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		G	C	M	M	A	P	T	A	L	G	S	I	I	A	K	S	M	V	T	Q	V	L	R	R	L	G	Y	R	H	T	L	V	G	I	T	V	I	I	G	L	M	I	A	Q	F	S	L	Q	S	P	A	M	A	I	W	M	L	I	L	P
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		L	F	I	L	G	M	A	M	S	T	Q	F	T	A	M	N	T	I	T	L	A	D	L	T	D	D	N	A	S	S	G	N	S	V	L	A	V	T	Q	Q	L	S	I	S	L	G	V	A	V	S	A	A	V	L	R	V	Y	E	G	M
Secondary structure
SS confidence
Disorder																					?	?		?	?				?	?	?																										?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.
Sequence		E	G	T	T	T	V	E	Q	F	H	Y	T	F	I	T	M	G	I	I	T	V	A	S	A	A	M	F	M	L	L	K	T	T	D	G	N	N	L	I	K	R	Q	R	K	S	K	P	N	R	V	P	S	E	S	E
Secondary structure
SS confidence
Disorder		?	?	?	?																												?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1pw4 chain A Region: 1 - 467 Aligned: 412 Modelled: 413 Confidence: 100.0% Identity: 11% Fold: MFS general substrate transporter Superfamily: MFS general substrate transporter Family: Glycerol-3-phosphate transporter Phyre2 PDB 2gfp chain A Region: 12 - 442 Aligned: 370 Modelled: 379 Confidence: 100.0% Identity: 19% PDB header:membrane protein Chain: A: PDB Molecule:multidrug resistance protein d; PDBTitle: structure of the multidrug transporter emrd from2 escherichia coli Phyre2 PDB 2xut chain C Region: 12 - 448 Aligned: 437 Modelled: 437 Confidence: 100.0% Identity: 11% PDB header:transport protein Chain: C: PDB Molecule:proton/peptide symporter family protein; PDBTitle: crystal structure of a proton dependent oligopeptide (pot)2 family transporter. Phyre2 PDB 3o7p chain A Region: 7 - 453 Aligned: 407 Modelled: 422 Confidence: 100.0% Identity: 13% PDB header:transport protein Chain: A: PDB Molecule:l-fucose-proton symporter; PDBTitle: crystal structure of the e.coli fucose:proton symporter, fucp (n162a) Phyre2 PDB 1pv7 chain A Region: 1 - 466 Aligned: 407 Modelled: 424 Confidence: 100.0% Identity: 13% Fold: MFS general substrate transporter Superfamily: MFS general substrate transporter Family: LacY-like proton/sugar symporter Phyre2 PDB 3b9y chain A Region: 255 - 475 Aligned: 208 Modelled: 221 Confidence: 85.6% Identity: 10% PDB header:transport protein Chain: A: PDB Molecule:ammonium transporter family rh-like protein; PDBTitle: crystal structure of the nitrosomonas europaea rh protein Phyre2 PDB 3hd6 chain A Region: 310 - 475 Aligned: 161 Modelled: 161 Confidence: 64.8% Identity: 9% PDB header:membrane protein, transport protein Chain: A: PDB Molecule:ammonium transporter rh type c; PDBTitle: crystal structure of the human rhesus glycoprotein rhcg Phyre2 PDB 3qnq chain D Region: 424 - 468 Aligned: 45 Modelled: 45 Confidence: 45.9% Identity: 9% PDB header:membrane protein, transport protein Chain: D: PDB Molecule:pts system, cellobiose-specific iic component; PDBTitle: crystal structure of the transporter chbc, the iic component from the2 n,n'-diacetylchitobiose-specific phosphotransferase system Phyre2 PDB 3cx5 chain C domain 2 Region: 7 - 67 Aligned: 61 Modelled: 61 Confidence: 14.7% Identity: 11% Fold: Heme-binding four-helical bundle Superfamily: Transmembrane di-heme cytochromes Family: Cytochrome b of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase) Phyre2 PDB 2g9p chain A Region: 65 - 78 Aligned: 14 Modelled: 14 Confidence: 14.1% Identity: 36% PDB header:antimicrobial protein Chain: A: PDB Molecule:antimicrobial peptide latarcin 2a; PDBTitle: nmr structure of a novel antimicrobial peptide, latarcin 2a,2 from spider (lachesana tarabaevi) venom Phyre2 PDB 2qjk chain M Region: 7 - 67 Aligned: 61 Modelled: 61 Confidence: 10.2% Identity: 11% PDB header:electron transport Chain: M: PDB Molecule:cytochrome b; PDBTitle: crystal structure analysis of mutant rhodobacter2 sphaeroides bc1 with stigmatellin and antimycin Phyre2 PDB 2oar chain A domain 1 Region: 391 - 475 Aligned: 81 Modelled: 85 Confidence: 8.9% Identity: 9% Fold: Gated mechanosensitive channel Superfamily: Gated mechanosensitive channel Family: Gated mechanosensitive channel Phyre2 PDB 3cx5 chain N Region: 7 - 67 Aligned: 61 Modelled: 61 Confidence: 8.1% Identity: 11% PDB header:oxidoreductase Chain: N: PDB Molecule:cytochrome b; PDBTitle: structure of complex iii with bound cytochrome c in reduced2 state and definition of a minimal core interface for3 electron transfer. Phyre2 PDB 2jo1 chain A Region: 432 - 475 Aligned: 38 Modelled: 44 Confidence: 7.6% Identity: 18% PDB header:hydrolase regulator Chain: A: PDB Molecule:phospholemman; PDBTitle: structure of the na,k-atpase regulatory protein fxyd1 in2 micelles Phyre2 PDB 1q90 chain B Region: 7 - 67 Aligned: 61 Modelled: 61 Confidence: 7.4% Identity: 8% Fold: Heme-binding four-helical bundle Superfamily: Transmembrane di-heme cytochromes Family: Cytochrome b of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase) Phyre2 PDB 2knc chain A Region: 429 - 470 Aligned: 42 Modelled: 42 Confidence: 6.6% Identity: 5% PDB header:cell adhesion Chain: A: PDB Molecule:integrin alpha-iib; PDBTitle: platelet integrin alfaiib-beta3 transmembrane-cytoplasmic2 heterocomplex Phyre2 1 d1pw4a_ 2 c2gfpA_ 3 c2xutC_ 4 c3o7pA_ 5 d1pv7a_ 6 c3b9yA_ 7 c3hd6A_ 8 c3qnqD_ 9 d3cx5c2 10 c2g9pA_ 11 c2qjkM_ 12 d2oara1 13 c3cx5N_ 14 c2jo1A_ 15 d1q90b_ 16 c2kncA_

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1pw4a_ 100.0 11 Fold:MFS general substrate transporter Superfamily:MFS general substrate transporter Family:Glycerol-3-phosphate transporter 2 c2gfpA_ 100.0 19 PDB header:membrane protein Chain: A: PDB Molecule:multidrug resistance protein d; PDBTitle: structure of the multidrug transporter emrd from2 escherichia coli 3 c2xutC_ 100.0 11 PDB header:transport protein Chain: C: PDB Molecule:proton/peptide symporter family protein; PDBTitle: crystal structure of a proton dependent oligopeptide (pot)2 family transporter. 4 c3o7pA_ 100.0 13 PDB header:transport protein Chain: A: PDB Molecule:l-fucose-proton symporter; PDBTitle: crystal structure of the e.coli fucose:proton symporter, fucp (n162a) 5 d1pv7a_ 100.0 13 Fold:MFS general substrate transporter Superfamily:MFS general substrate transporter Family:LacY-like proton/sugar symporter 6 c3b9yA_ 85.6 10 PDB header:transport protein Chain: A: PDB Molecule:ammonium transporter family rh-like protein; PDBTitle: crystal structure of the nitrosomonas europaea rh protein 7 c3hd6A_ 64.8 9 PDB header:membrane protein, transport protein Chain: A: PDB Molecule:ammonium transporter rh type c; PDBTitle: crystal structure of the human rhesus glycoprotein rhcg 8 c3qnqD_ 45.9 9 PDB header:membrane protein, transport protein Chain: D: PDB Molecule:pts system, cellobiose-specific iic component; PDBTitle: crystal structure of the transporter chbc, the iic component from the2 n,n'-diacetylchitobiose-specific phosphotransferase system 9 d3cx5c2 14.7 11 Fold:Heme-binding four-helical bundle Superfamily:Transmembrane di-heme cytochromes Family:Cytochrome b of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase) 10 c2g9pA_ 14.1 36 PDB header:antimicrobial protein Chain: A: PDB Molecule:antimicrobial peptide latarcin 2a; PDBTitle: nmr structure of a novel antimicrobial peptide, latarcin 2a,2 from spider (lachesana tarabaevi) venom 11 c2qjkM_ 10.2 11 PDB header:electron transport Chain: M: PDB Molecule:cytochrome b; PDBTitle: crystal structure analysis of mutant rhodobacter2 sphaeroides bc1 with stigmatellin and antimycin 12 d2oara1 8.9 9 Fold:Gated mechanosensitive channel Superfamily:Gated mechanosensitive channel Family:Gated mechanosensitive channel 13 c3cx5N_ 8.1 11 PDB header:oxidoreductase Chain: N: PDB Molecule:cytochrome b; PDBTitle: structure of complex iii with bound cytochrome c in reduced2 state and definition of a minimal core interface for3 electron transfer. 14 c2jo1A_ 7.6 18 PDB header:hydrolase regulator Chain: A: PDB Molecule:phospholemman; PDBTitle: structure of the na,k-atpase regulatory protein fxyd1 in2 micelles 15 d1q90b_ 7.4 8 Fold:Heme-binding four-helical bundle Superfamily:Transmembrane di-heme cytochromes Family:Cytochrome b of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase) 16 c2kncA_ 6.6 5 PDB header:cell adhesion Chain: A: PDB Molecule:integrin alpha-iib; PDBTitle: platelet integrin alfaiib-beta3 transmembrane-cytoplasmic2 heterocomplex

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0