Job Description P55138 Confidence 100.00% Date Thu Jan 5 12:06:08 GMT 2012 Rank 14 Aligned Residues 426 % Identity 20% Template c3ezwD_ PDB info PDB header:transferase Chain: D: PDB Molecule:glycerol kinase; PDBTitle: crystal structure of a hyperactive escherichia coli glycerol kinase2 mutant gly230 --> asp obtained using microfluidic crystallization3 devices Resolution 2.00 Å

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		2	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60	.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.
Predicted Secondary structure
Query SS confidence
Query Sequence		S	K	K	Y	I	I	G	I	D	G	G	S	Q	S	T	K	V	V	M	Y	D	L	E	G	N	V	V	C	E	G	K	G	L	L	Q	P	M	H	T	P	D	A	D	T	A	E	H	P	D	D	D	L	W	A	S	L	C	F	A	G	H	D	L	M	S	Q	F	A	G	N	K	E	D	I	V	G	I	G	L	G
Query Conservation
Alig confidence																																				.
Template Conservation																																				.
Template Sequence		E	K	K	Y	I	V	A	L	D	Q	G	T	T	S	S	R	A	V	V	M	D	H	D	A	N	I	I	S	V	S	Q	R	E	F	.	E	Q	I	Y	P	K	P	G	W	V	E	H	D	P	M	E	I	W	A	T	Q	S	S	T	L	V	E	V	L	A	K	A	D	I	S	S	D	Q	I	A	A	I	G	I	T
Template Known Secondary structure													S	S									T	T												.			B			S	T	T																								T	T			G	G	G
Template Predicted Secondary structure																																				.
Template SS confidence
		2	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.		.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60	.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80
		82	.	.	.	.	.	.	.	90	.	.		.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110																																	.	.	.	.	.	.	.	.	.	120	.
Predicted Secondary structure													.																			.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.												.	.	.	.	.	.	.
Query SS confidence													.																			.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.												.	.	.	.	.	.	.
Query Sequence		S	I	R	C	C	R	A	L	L	K	A	.	D	G	T	P	A	A	P	L	I	S	W	Q	D	A	R	V	T	R	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	P	Y	E	H	T	N	P	D	V	A	Y	.	.	.	.	.	.	.
Query Conservation													.																			.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.												.	.	.	.	.	.	.
Alig confidence													.																			.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.												.	.	.	.	.	.	.
Template Conservation
Template Sequence		N	Q	R	E	T	T	I	V	W	E	K	E	T	G	K	P	I	Y	N	A	I	V	W	Q	C	R	R	T	A	E	I	C	E	H	L	K	R	D	G	L	E	D	Y	I	R	S	N	T	G	L	V	I	D	P	Y	F	S	G	T	K	V	K	W	I	L	D	H	V	E	G	S	R	E	R	A	R	R	G	E	L
Template Known Secondary structure				S	S		B					T	T	T			B	S	S					T	T			T	T										T	T										S			S	S	T	T	S												S	T	T							T	T	S
Template Predicted Secondary structure
Template SS confidence
		81	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120	.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160
			122	.	.	.	.	.	.	.	130	.	.	.	.	.			.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180	.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.
Predicted Secondary structure		.															.	.
Query SS confidence		.															.	.
Query Sequence		.	V	T	S	F	S	G	Y	L	T	H	R	L	T	G	.	.	E	F	K	D	N	I	A	N	Y	F	G	Q	W	P	V	D	Y	K	S	W	A	W	S	E	D	A	A	V	M	D	K	F	N	I	P	R	H	M	L	F	D	V	Q	M	P	G	T	V	L	G	H	I	T	P	Q	A	A	L	A	T	H	F	P
Query Conservation		.															.	.
Alig confidence		.															.	.																										.	.																											.	.	.	.	.	.	.	.	.	.
Template Conservation																																												.	.																											.	.	.	.	.	.	.	.	.	.
Template Sequence		L	F	G	T	V	D	T	W	L	I	W	K	M	T	Q	G	R	V	H	V	T	D	Y	T	N	A	S	R	T	M	L	F	N	I	H	T	L	D	W	D	D	K	.	.	M	L	E	V	L	D	I	P	R	E	M	L	P	E	V	R	R	S	S	E	V	Y	G	Q	T	N	.	.	.	.	.	.	.	.	.	.
Template Known Secondary structure															T	T	T	S										G	G	G	S	S			T	T	T	T						.	.					T	T			G	G	G							S									.	.	.	.	.	.	.	.	.	.
Template Predicted Secondary structure																																												.	.																											.	.	.	.	.	.	.	.	.	.
Template SS confidence
		161	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180	.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.			.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.
		199	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240	.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.				.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.
Predicted Secondary structure																																																												.	.	.
Query SS confidence																																																												.	.	.
Query Sequence		A	G	L	P	V	V	C	T	T	S	D	K	P	V	E	A	L	G	A	G	L	L	D	D	E	T	A	V	I	S	L	G	T	Y	I	A	L	M	M	N	G	K	A	L	P	K	D	P	V	A	Y	W	P	I	M	S	S	I	.	.	.	P	Q	T	L	L	Y	E	G	Y	G	I	R	K	G	M	W	T	V	S
Query Conservation																																																												.	.	.
Alig confidence		.																																																										.	.	.
Template Conservation		.
Template Sequence		.	R	I	P	I	S	G	I	A	G	D	Q	Q	A	A	L	F	G	Q	L	C	V	K	E	G	M	A	K	N	T	Y	G	T	G	C	F	M	L	M	N	T	G	E	K	A	V	K	S	E	N	G	L	L	T	T	I	A	C	G	P	T	G	E	V	N	Y	A	L	E	G	A	V	F	M	A	G	A	S	I	Q
Template Known Secondary structure		.																		T	T			S	T	T							S	S	S								T	T	S					S	S	S									T	T	S											S		S
Template Predicted Secondary structure		.
Template SS confidence
			236	.	.	.	240	.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300	.	.	.	.	.	.	.	.	.	310	.	.	.	.
		276	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300	.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.				.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.
Predicted Secondary structure																																													.	.	.
Query SS confidence																																													.	.	.
Query Sequence		W	L	R	D	M	L	G	E	S	L	I	Q	D	A	R	A	Q	D	L	S	P	E	D	L	L	N	K	K	A	S	C	V	P	P	G	C	N	G	L	M	T	V	L	.	.	.	D	W	L	T	N	P	W	E	P	Y	K	R	G	I	M	I	G	F	D	S	S	M	D	Y	A	W	I	Y	R	S	I	L	E	S
Query Conservation																																													.	.	.
Alig confidence											.	.	.	.	.	.	.	.	.	.	.	.	.	.	.																				.	.	.
Template Conservation											.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Sequence		W	L	R	D	E	M	K	L	I	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	D	S	E	Y	F	A	T	K	V	Q	N	T	N	G	V	Y	V	V	P	A	F	T	G	L	G	A	P	Y	W	D	P	Y	A	R	G	A	I	F	G	L	T	R	G	V	N	A	N	H	I	I	R	A	T	L	E	S
Template Known Secondary structure						T	S				.	.	.	.	.	.	.	.	.	.	.	.	.	.	.								T	S	S	S		T	T							T	T		B		T	T	T		B	T	T										T	T
Template Predicted Secondary structure											.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template SS confidence
		315	.	.	.	.	320	.	.	.																.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360	.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.
		353	.	.	.	.	.	.	360	.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420	.	.	.	.	.	.	.	.	.	430	.	.
Predicted Secondary structure
Query SS confidence
Query Sequence		V	A	L	T	L	K	N	N	Y	D	N	M	C	N	E	M	N	H	F	A	K	H	V	I	I	T	G	G	G	S	N	S	D	L	F	M	Q	I	F	A	D	V	F	N	L	P	A	R	R	N	A	I	N	G	C	A	S	L	G	A	A	I	N	T	A	V	G	L	G	L	Y	P	D	Y	A	T	A	V	D	N
Query Conservation
Alig confidence																																																			.	.	.																									.	.	.	.
Template Conservation																																																			.	.	.																									.	.	.	.
Template Sequence		I	A	Y	Q	T	R	D	V	L	E	A	M	Q	A	D	S	G	I	R	L	H	A	L	R	V	D	G	G	A	V	A	N	N	F	L	M	Q	F	Q	S	D	I	L	G	T	R	V	E	R	.	.	.	P	E	V	T	A	L	G	A	A	Y	L	A	G	L	A	V	G	F	W	Q	N	L	D	E	.	.	.	.
Template Known Secondary structure																						S						S	G	G	G	G													T	S					.	.	.																	T	S	S	S					.	.	.	.
Template Predicted Secondary structure																																																			.	.	.																									.	.	.	.
Template SS confidence
		384	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420	.	.	.	.	.	.	.	.	.	430	.	.				.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.
		433	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.
Predicted Secondary structure
Query SS confidence
Query Sequence		M	V	R	V	K	D	I	F	I	P	I	E	S	N	A	K	R	Y	D	A	M	N	K	G	I	F	K	D	L	T	K	H	T
Query Conservation
Alig confidence																						.			.
Template Conservation																						.			.
Template Sequence		L	Q	E	I	E	R	E	F	R	P	G	I	E	T	T	E	R	N	Y	R	.	Y	A	.	G	W	K	K	A	V	K	R	A
Template Known Secondary structure			T										S									.			.
Template Predicted Secondary structure																						.			.
Template SS confidence
		460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.		480	.		.	.	.	.	.	.	.	.	490