Job Description P77212 Confidence 100.00% Date Thu Jan 5 12:26:22 GMT 2012 Rank 23 Aligned Residues 426 % Identity 29% Template c1geuA_ PDB info PDB header:oxidoreductase(flavoenzyme) Chain: A: PDB Molecule:glutathione reductase; PDBTitle: anatomy of an engineered nad-binding site Resolution 2.20 Å

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		2	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60	.	.	.	.	.	.	.	.	.	70	.
Predicted Secondary structure																																																																								.	.	.	.	.	.	.	.	.	.
Query SS confidence																																																																								.	.	.	.	.	.	.	.	.	.
Query Sequence		N	K	Y	Q	A	V	I	I	G	F	G	K	A	G	K	T	L	A	V	T	L	A	K	A	G	W	R	V	A	L	I	E	Q	S	N	A	M	Y	G	G	T	C	I	N	I	G	C	I	P	T	K	T	L	V	H	D	A	Q	Q	H	T	D	F	V	R	A	I	Q	R	K	.	.	.	.	.	.	.	.	.	.
Query Conservation																																																																								.	.	.	.	.	.	.	.	.	.
Alig confidence																																									.	.																														.	.	.	.	.	.	.	.	.	.
Template Conservation																																									.	.
Template Sequence		K	H	Y	D	Y	I	A	I	G	G	G	S	G	G	I	A	S	I	N	R	A	A	M	Y	G	Q	K	C	A	L	I	E	A	K	E	L	G	G	T	.	.	C	V	N	V	G	C	V	P	K	K	V	M	W	H	A	A	Q	I	R	E	A	I	H	M	Y	G	P	D	Y	G	F	D	T	T	I	N	K	F	N
Template Known Secondary structure												S												T	T	T								S	S		T	T			.	.					S																			T	T	T	G	G	G	T
Template Predicted Secondary structure																																									.	.
Template SS confidence
		3	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.			.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60	.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80
									72	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120	.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.
Predicted Secondary structure		.	.	.	.	.	.	.
Query SS confidence		.	.	.	.	.	.	.
Query Sequence		.	.	.	.	.	.	.	N	E	V	V	N	F	L	R	N	K	N	F	H	N	L	A	D	M	P	N	I	D	V	I	D	G	Q	A	E	F	I	N	N	H	S	L	R	V	H	R	P	E	G	N	L	E	I	H	G	E	K	I	F	I	N	T	G	A	Q	T	V	V	P	P	I	P	G	I	T	T	T	P	G
Query Conservation		.	.	.	.	.	.	.
Alig confidence		.	.	.	.	.	.	.																																							.	.	.	.																														.	.
Template Conservation																																															.	.	.	.																														.	.
Template Sequence		W	E	T	L	I	A	S	R	T	A	Y	I	D	R	I	H	T	S	Y	E	N	V	L	G	K	N	N	V	D	V	I	K	G	F	A	R	F	V	D	A	K	T	L	E	V	.	.	.	.	N	G	E	T	I	T	A	D	H	I	L	I	A	T	G	G	R	P	S	H	P	D	I	P	G	V	E	Y	G	.	.
Template Known Secondary structure																											T	T						S							T	T					.	.	.	.	T	T																					S	T	T	G	G	G	S	.	.
Template Predicted Secondary structure																																															.	.	.	.																														.	.
Template SS confidence
		81	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120	.	.	.	.	.					.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.
		145	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180	.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.
Predicted Secondary structure
Query SS confidence
Query Sequence		V	Y	D	S	T	G	L	L	N	L	K	E	L	P	G	H	L	G	I	L	G	G	G	Y	I	G	V	E	F	A	S	M	F	A	N	F	G	S	K	V	T	I	L	E	A	A	S	L	F	L	P	R	E	D	R	D	I	A	D	N	I	A	T	I	L	R	D	Q	G	V	D	I	I	L	N	A	H	V	E	R
Query Conservation
Alig confidence		.
Template Conservation		.
Template Sequence		.	I	D	S	D	G	F	F	A	L	P	A	L	P	E	R	V	A	V	V	G	A	G	Y	I	G	V	E	L	G	G	V	I	N	G	L	G	A	K	T	H	L	F	E	M	F	D	A	P	L	P	S	F	D	P	M	I	S	E	T	L	V	E	V	M	N	A	E	G	P	Q	L	H	T	N	A	I	P	K	A
Template Known Secondary structure		.	B							T		S	S			S								S													T	T								S	S	S	S	S	S	T	T	S																						S
Template Predicted Secondary structure		.
Template SS confidence
			155	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180	.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.
		225	.	.	.	.	230	.			.	.	.	.	.	.	.	.	240	.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300	.	.
Predicted Secondary structure									.	.
Query SS confidence									.	.
Query Sequence		I	S	H	H	E	N	Q	.	.	V	Q	V	H	S	E	H	A	Q	L	A	V	D	A	L	L	I	A	S	G	R	Q	P	A	T	A	S	L	H	P	E	N	A	G	I	A	V	N	E	R	G	A	I	V	V	D	K	R	L	H	T	T	A	D	N	I	W	A	M	G	D	V	T	G	G	L	Q	F	T	Y	I
Query Conservation									.	.
Alig confidence									.	.
Template Conservation
Template Sequence		V	V	K	N	T	D	G	S	L	T	L	E	L	E	D	G	R	S	E	T	V	D	C	L	I	W	A	I	G	R	E	P	A	N	D	N	I	N	L	E	A	A	G	V	K	T	N	E	K	G	Y	I	V	V	D	K	Y	Q	N	T	N	I	E	G	I	Y	A	V	G	D	N	T	G	A	V	E	L	T	P	V
Template Known Secondary structure						T	T	S							T	T	S						S						S							S	T	T						T			B		T	T	S		B				T	T	S	B		S	S	T	T					G	G	G	G	T	S
Template Predicted Secondary structure
Template SS confidence
		234	.	.	.	.	.	240	.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300	.	.	.	.	.	.	.	.	.	310	.	.	.
		303	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.			.	.	.	.	.	360	.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380
Predicted Secondary structure																																																						.	.
Query SS confidence																																																						.	.
Query Sequence		S	L	D	D	Y	R	I	V	R	D	E	L	L	G	E	G	K	R	S	T	D	D	R	K	N	V	P	Y	S	V	F	M	T	P	P	L	S	R	V	G	M	T	E	E	Q	A	R	E	S	G	A	D	.	.	I	Q	V	V	T	L	P	V	A	A	I	P	R	A	R	V	M	N	D	T	R	G	V	L	K	A
Query Conservation																																																						.	.
Alig confidence																																																						.	.
Template Conservation
Template Sequence		A	V	A	A	G	R	R	L	S	E	R	L	F	N	N	K	P	D	E	H	L	D	Y	S	N	I	P	T	V	V	F	S	H	P	P	I	G	T	V	G	L	T	E	P	Q	A	R	E	Q	Y	G	D	D	Q	V	K	V	Y	K	S	S	F	T	A	M	Y	T	A	V	T	T	H	R	Q	P	C	R	M	K	L
Template Known Secondary structure															T	T		T	T						S	S								S	S																		G	G	G															T	S	S	S
Template Predicted Secondary structure
Template SS confidence
		314	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360	.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.
		381	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420	.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.
Predicted Secondary structure
Query SS confidence
Query Sequence		I	V	D	N	K	T	Q	R	M	L	G	A	S	L	L	C	V	D	S	H	E	M	I	N	I	V	K	M	V	M	D	A	G	L	P	Y	S	I	L	R	D	Q	I	F	T	H	P	S	M	S	E	S	L	N	D	L
Query Conservation
Alig confidence
Template Conservation
Template Sequence		V	C	V	G	S	E	E	K	I	V	G	I	H	G	I	G	F	G	M	D	E	M	L	Q	G	F	A	V	A	L	K	M	G	A	T	K	K	D	F	D	N	T	V	A	I	H	P	T	A	A	E	E	F	V	T	M
Template Known Secondary structure					T	T	T	T									S	T	T														T	T		B						T	S				S	S		S	G	G	G	G	G	T
Template Predicted Secondary structure
Template SS confidence
		394	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420	.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.