Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	I	L	D	T	V	D	E	K	K	K	G	V	H	T	R	Y	L	I	L	L	I	I	F	I	V	T	A	V	N	Y	A	D	R	A	T	L	S	I	A	G	T	E	V	A	K	E	L	Q	L	S	A	V	S	M	G	Y	I	F	S
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		A	F	G	W	A	Y	L	L	M	Q	I	P	G	G	W	L	L	D	K	F	G	S	K	K	V	Y	T	Y	S	L	F	F	W	S	L	F	T	F	L	Q	G	F	V	D	M	F	P	L	A	W	A	G	I	S	M	F	F	M	R	F
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		M	L	G	F	S	E	A	P	S	F	P	A	N	A	R	I	V	A	A	W	F	P	T	K	E	R	G	T	A	S	A	I	F	N	S	A	Q	Y	F	S	L	A	L	F	S	P	L	L	G	W	L	T	F	A	W	G	W	E	H	V
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		F	T	V	M	G	V	I	G	F	V	L	T	A	L	W	I	K	L	I	H	N	P	T	D	H	P	R	M	S	A	E	E	L	K	F	I	S	E	N	G	A	V	V	D	M	D	H	K	K	P	G	S	A	A	A	S	G	P	K	L
Secondary structure
SS confidence
Disorder																												?											?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		H	Y	I	K	Q	L	L	S	N	R	M	M	L	G	V	F	F	G	Q	Y	F	I	N	T	I	T	W	F	F	L	T	W	F	P	I	Y	L	V	Q	E	K	G	M	S	I	L	K	V	G	L	V	A	S	I	P	A	L	C	G	F
Secondary structure
SS confidence
Disorder																																											?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		A	G	G	V	L	G	G	V	F	S	D	Y	L	I	K	R	G	L	S	L	T	L	A	R	K	L	P	I	V	L	G	M	L	L	A	S	T	I	I	L	C	N	Y	T	N	N	T	T	L	V	V	M	L	M	A	L	A	F	F	G
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		K	G	F	G	A	L	G	W	P	V	I	S	D	T	A	P	K	E	I	V	G	L	C	G	G	V	F	N	V	F	G	N	V	A	S	I	V	T	P	L	V	I	G	Y	L	V	S	E	L	H	S	F	N	A	A	L	V	F	V	G
Secondary structure
SS confidence
Disorder																?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.
Sequence		C	S	A	L	M	A	M	V	C	Y	L	F	V	V	G	D	I	K	R	M	E	L	Q	K
Secondary structure
SS confidence
Disorder																		?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1pw4 chain A Region: 3 - 444 Aligned: 418 Modelled: 424 Confidence: 100.0% Identity: 20% Fold: MFS general substrate transporter Superfamily: MFS general substrate transporter Family: Glycerol-3-phosphate transporter Phyre2 PDB 3o7p chain A Region: 14 - 435 Aligned: 409 Modelled: 415 Confidence: 100.0% Identity: 10% PDB header:transport protein Chain: A: PDB Molecule:l-fucose-proton symporter; PDBTitle: crystal structure of the e.coli fucose:proton symporter, fucp (n162a) Phyre2 PDB 2gfp chain A Region: 19 - 425 Aligned: 369 Modelled: 380 Confidence: 100.0% Identity: 17% PDB header:membrane protein Chain: A: PDB Molecule:multidrug resistance protein d; PDBTitle: structure of the multidrug transporter emrd from2 escherichia coli Phyre2 PDB 1pv7 chain A Region: 16 - 444 Aligned: 404 Modelled: 412 Confidence: 100.0% Identity: 13% Fold: MFS general substrate transporter Superfamily: MFS general substrate transporter Family: LacY-like proton/sugar symporter Phyre2 PDB 2xut chain C Region: 21 - 434 Aligned: 409 Modelled: 414 Confidence: 100.0% Identity: 12% PDB header:transport protein Chain: C: PDB Molecule:proton/peptide symporter family protein; PDBTitle: crystal structure of a proton dependent oligopeptide (pot)2 family transporter. Phyre2 PDB 3b9y chain A Region: 2 - 244 Aligned: 231 Modelled: 243 Confidence: 64.5% Identity: 9% PDB header:transport protein Chain: A: PDB Molecule:ammonium transporter family rh-like protein; PDBTitle: crystal structure of the nitrosomonas europaea rh protein Phyre2 PDB 2g9p chain A Region: 72 - 85 Aligned: 14 Modelled: 14 Confidence: 14.5% Identity: 43% PDB header:antimicrobial protein Chain: A: PDB Molecule:antimicrobial peptide latarcin 2a; PDBTitle: nmr structure of a novel antimicrobial peptide, latarcin 2a,2 from spider (lachesana tarabaevi) venom Phyre2 PDB 2wwa chain A Region: 4 - 81 Aligned: 69 Modelled: 78 Confidence: 7.4% Identity: 12% PDB header:ribosome Chain: A: PDB Molecule:sec sixty-one protein homolog; PDBTitle: cryo-em structure of idle yeast ssh1 complex bound to the2 yeast 80s ribosome Phyre2 PDB 3pro chain C domain 1 Region: 40 - 81 Aligned: 42 Modelled: 42 Confidence: 5.9% Identity: 7% Fold: Alpha-lytic protease prodomain-like Superfamily: Alpha-lytic protease prodomain Family: Alpha-lytic protease prodomain Phyre2 PDB 2rdd chain B Region: 418 - 444 Aligned: 27 Modelled: 27 Confidence: 5.9% Identity: 11% PDB header:membrane protein/transport protein Chain: B: PDB Molecule:upf0092 membrane protein yajc; PDBTitle: x-ray crystal structure of acrb in complex with a novel2 transmembrane helix. Phyre2 1 d1pw4a_ 2 c3o7pA_ 3 c2gfpA_ 4 d1pv7a_ 5 c2xutC_ 6 c3b9yA_ 7 c2g9pA_ 8 c2wwaA_ 9 d3proc1 10 c2rddB_

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1pw4a_ 100.0 20 Fold:MFS general substrate transporter Superfamily:MFS general substrate transporter Family:Glycerol-3-phosphate transporter 2 c3o7pA_ 100.0 10 PDB header:transport protein Chain: A: PDB Molecule:l-fucose-proton symporter; PDBTitle: crystal structure of the e.coli fucose:proton symporter, fucp (n162a) 3 c2gfpA_ 100.0 17 PDB header:membrane protein Chain: A: PDB Molecule:multidrug resistance protein d; PDBTitle: structure of the multidrug transporter emrd from2 escherichia coli 4 d1pv7a_ 100.0 13 Fold:MFS general substrate transporter Superfamily:MFS general substrate transporter Family:LacY-like proton/sugar symporter 5 c2xutC_ 100.0 12 PDB header:transport protein Chain: C: PDB Molecule:proton/peptide symporter family protein; PDBTitle: crystal structure of a proton dependent oligopeptide (pot)2 family transporter. 6 c3b9yA_ 64.5 9 PDB header:transport protein Chain: A: PDB Molecule:ammonium transporter family rh-like protein; PDBTitle: crystal structure of the nitrosomonas europaea rh protein 7 c2g9pA_ 14.5 43 PDB header:antimicrobial protein Chain: A: PDB Molecule:antimicrobial peptide latarcin 2a; PDBTitle: nmr structure of a novel antimicrobial peptide, latarcin 2a,2 from spider (lachesana tarabaevi) venom 8 c2wwaA_ 7.4 12 PDB header:ribosome Chain: A: PDB Molecule:sec sixty-one protein homolog; PDBTitle: cryo-em structure of idle yeast ssh1 complex bound to the2 yeast 80s ribosome 9 d3proc1 5.9 7 Fold:Alpha-lytic protease prodomain-like Superfamily:Alpha-lytic protease prodomain Family:Alpha-lytic protease prodomain 10 c2rddB_ 5.9 11 PDB header:membrane protein/transport protein Chain: B: PDB Molecule:upf0092 membrane protein yajc; PDBTitle: x-ray crystal structure of acrb in complex with a novel2 transmembrane helix.

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0