Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	V	I	G	P	F	I	N	A	S	A	V	L	L	G	G	V	L	G	A	L	L	S	Q	R	L	P	E	R	I	R	V	S	M	T	S	I	F	G	L	A	S	L	G	I	G	I	L	L	V	V	K	C	A	N	L	P	A	M	V
Secondary structure
SS confidence
Disorder		?	?	?																																																?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		L	A	T	L	L	G	A	L	I	G	E	I	C	L	L	E	K	G	V	N	T	A	V	A	K	A	Q	N	L	F	R	H	S	R	K	K	P	A	H	E	S	F	I	Q	N	Y	V	A	I	I	V	L	F	C	A	S	G	T	G	I
Secondary structure
SS confidence
Disorder																																?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		F	G	A	M	N	E	G	M	T	G	D	P	S	I	L	I	A	K	S	F	L	D	F	F	T	A	M	I	F	A	C	S	L	G	I	A	V	S	V	I	S	I	P	L	L	I	I	Q	L	T	L	A	W	A	A	A	L	I	L	P
Secondary structure
SS confidence
Disorder																																																												?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.
Sequence		L	T	T	P	S	M	M	A	D	F	S	A	V	G	G	L	L	L	L	A	T	G	L	R	I	C	G	I	K	M	F	P	V	V	N	M	L	P	A	L	L	L	A	M	P	L	S	A	A	W	T	A	W	F	A
Secondary structure
SS confidence
Disorder																																		?																						?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1kpl chain A Region: 11 - 92 Aligned: 82 Modelled: 82 Confidence: 12.6% Identity: 15% Fold: Clc chloride channel Superfamily: Clc chloride channel Family: Clc chloride channel Phyre2 PDB 1rh1 chain A domain 2 Region: 129 - 191 Aligned: 63 Modelled: 63 Confidence: 11.6% Identity: 14% Fold: Toxins' membrane translocation domains Superfamily: Colicin Family: Colicin Phyre2 PDB 1dxs chain A Region: 25 - 38 Aligned: 14 Modelled: 14 Confidence: 11.0% Identity: 21% Fold: SAM domain-like Superfamily: SAM/Pointed domain Family: SAM (sterile alpha motif) domain Phyre2 PDB 2o34 chain A domain 1 Region: 72 - 126 Aligned: 55 Modelled: 55 Confidence: 10.7% Identity: 7% Fold: T-fold Superfamily: ApbE-like Family: DVU1097-like Phyre2 PDB 2uwj chain G Region: 71 - 106 Aligned: 36 Modelled: 36 Confidence: 9.2% Identity: 3% PDB header:chaperone Chain: G: PDB Molecule:type iii export protein pscg; PDBTitle: structure of the heterotrimeric complex which regulates2 type iii secretion needle formation Phyre2 PDB 2ju0 chain B Region: 215 - 223 Aligned: 9 Modelled: 9 Confidence: 8.4% Identity: 56% PDB header:metal binding protein/signaling protein Chain: B: PDB Molecule:phosphatidylinositol 4-kinase pik1; PDBTitle: structure of yeast frequenin bound to pdtins 4-kinase Phyre2 PDB 3few chain X Region: 129 - 191 Aligned: 61 Modelled: 63 Confidence: 7.0% Identity: 15% PDB header:immune system Chain: X: PDB Molecule:colicin s4; PDBTitle: structure and function of colicin s4, a colicin with a2 duplicated receptor binding domain Phyre2 PDB 1aa7 chain A Region: 83 - 118 Aligned: 36 Modelled: 36 Confidence: 6.3% Identity: 11% Fold: Influenza virus matrix protein M1 Superfamily: Influenza virus matrix protein M1 Family: Influenza virus matrix protein M1 Phyre2 PDB 1c7q chain A Region: 76 - 100 Aligned: 25 Modelled: 25 Confidence: 6.2% Identity: 20% Fold: SIS domain Superfamily: SIS domain Family: Phosphoglucose isomerase, PGI Phyre2 1 d1kpla_ 2 d1rh1a2 3 d1dxsa_ 4 d2o34a1 5 c2uwjG_ 6 c2ju0B_ 7 c3fewX_ 8 d1aa7a_ 9 d1c7qa_

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1kpla_ 12.6 15 Fold:Clc chloride channel Superfamily:Clc chloride channel Family:Clc chloride channel 2 d1rh1a2 11.6 14 Fold:Toxins' membrane translocation domains Superfamily:Colicin Family:Colicin 3 d1dxsa_ 11.0 21 Fold:SAM domain-like Superfamily:SAM/Pointed domain Family:SAM (sterile alpha motif) domain 4 d2o34a1 10.7 7 Fold:T-fold Superfamily:ApbE-like Family:DVU1097-like 5 c2uwjG_ 9.2 3 PDB header:chaperone Chain: G: PDB Molecule:type iii export protein pscg; PDBTitle: structure of the heterotrimeric complex which regulates2 type iii secretion needle formation 6 c2ju0B_ 8.4 56 PDB header:metal binding protein/signaling protein Chain: B: PDB Molecule:phosphatidylinositol 4-kinase pik1; PDBTitle: structure of yeast frequenin bound to pdtins 4-kinase 7 c3fewX_ 7.0 15 PDB header:immune system Chain: X: PDB Molecule:colicin s4; PDBTitle: structure and function of colicin s4, a colicin with a2 duplicated receptor binding domain 8 d1aa7a_ 6.3 11 Fold:Influenza virus matrix protein M1 Superfamily:Influenza virus matrix protein M1 Family:Influenza virus matrix protein M1 9 d1c7qa_ 6.2 20 Fold:SIS domain Superfamily:SIS domain Family:Phosphoglucose isomerase, PGI

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0