Job Description P08204 Confidence 100.00% Date Thu Jan 5 11:00:58 GMT 2012 Rank 6 Aligned Residues 484 % Identity 19% Template c3flcX_ PDB info PDB header:transferase Chain: X: PDB Molecule:glycerol kinase; PDBTitle: crystal structure of the his-tagged h232r mutant of glycerol kinase2 from enterococcus casseliflavus with glycerol Resolution 1.85 Å

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		2	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60	.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.
Predicted Secondary structure
Query SS confidence
Query Sequence		A	I	A	I	G	L	D	F	G	S	D	S	V	R	A	L	A	V	D	C	A	T	G	E	E	I	A	T	S	V	E	W	Y	P	R	W	Q	K	G	Q	F	C	D	A	P	N	N	Q	F	R	H	H	P	R	D	Y	I	E	S	M	E	A	A	L	K	T	V	L	A	E	L	S	V	E	Q	R	A	A	V	V
Query Conservation
Alig confidence																					.															.	.	.	.	.	.																																				.
Template Conservation																					.															.	.	.	.	.	.																																				.
Template Sequence		N	Y	V	M	A	I	D	Q	G	T	T	S	S	R	A	I	I	F	D	.	R	N	G	K	K	I	G	S	S	Q	K	E	F	P	.	.	.	.	.	.	Q	Y	F	P	K	S	G	W	V	E	H	N	A	N	E	I	W	N	S	V	Q	S	V	I	A	G	A	F	I	E	S	G	I	R	P	.	E	A	I	A
Template Known Secondary structure											S	S									.	T	T	S												.	.	.	.	.	.		B		S	S	T	T																												G	.	G	G
Template Predicted Secondary structure																					.															.	.	.	.	.	.																																				.
Template SS confidence
		5	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.		.	.	.	.	.	.	30	.	.	.	.	.	.	.							.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60	.	.	.	.	.	.	.	.	.	70	.	.		.	.	.	.
		82	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.		.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120	.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160
Predicted Secondary structure																		.
Query SS confidence																		.
Query Sequence		G	I	G	V	D	S	T	G	S	T	P	A	P	I	D	A	.	D	G	N	V	L	A	L	R	P	E	F	A	E	N	P	N	A	M	F	V	L	W	K	D	H	T	A	V	E	E	A	E	E	I	T	R	L	C	H	A	P	G	N	V	D	Y	S	R	Y	I	G	G	I	Y	S	S	E	W	F	W	A	K	I
Query Conservation																		.
Alig confidence																		.						.	.	.	.	.	.	.	.	.	.	.																					.	.	.
Template Conservation																								.	.	.	.	.	.	.	.	.	.	.																					.	.	.
Template Sequence		G	I	G	I	T	N	Q	R	E	T	T	V	V	W	D	K	T	T	G	Q	P	I	.	.	.	.	.	.	.	.	.	.	.	A	N	A	I	V	W	Q	S	R	Q	S	S	P	I	A	D	Q	L	K	V	.	.	.	D	G	H	T	E	M	I	H	E	K	T	G	L	V	I	D	A	Y	F	S	A	T	K	V
Template Known Secondary structure									S	S							T	T	T			B	S	.	.	.	.	.	.	.	.	.	.	.	S					T	T			T	T										.	.	.	T	T										S					T	T	S
Template Predicted Secondary structure																								.	.	.	.	.	.	.	.	.	.	.																					.	.	.
Template SS confidence
		77	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.												.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.				.	120	.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.
		161	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.					.	.	.	180	.	.	.	.	.	.	.	.	.	190	.			.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.
Predicted Secondary structure																		.	.	.	.																.	.
Query SS confidence																		.	.	.	.																.	.
Query Sequence		L	H	V	T	R	Q	D	S	A	V	A	Q	S	A	A	S	.	.	.	.	W	I	E	L	C	D	W	V	P	A	L	L	S	G	T	.	.	T	R	P	Q	D	I	R	R	G	R	C	S	A	G	H	K	S	L	W	H	E	S	W	G	G	L	P	P	A	S	F	F	D	E	L	D	P	I	L	N	R	H	L
Query Conservation																		.	.	.	.																.	.
Alig confidence																		.	.	.	.																.	.											.	.	.	.	.	.																				.	.	.	.	.	.
Template Conservation																																																	.	.	.	.	.	.																				.	.	.	.	.	.
Template Sequence		R	W	L	L	D	N	I	E	G	A	Q	E	K	A	D	N	G	E	L	L	F	G	T	I	D	S	W	L	V	W	K	L	T	D	G	Q	V	H	V	T	D	Y	S	N	A	S	R	.	.	.	.	.	.	T	M	L	Y	N	I	H	K	L	E	W	D	Q	E	I	L	D	L	L	.	.	.	.	.	.	N	I
Template Known Secondary structure								S	T	T							T	T																T	T	T	S											T	.	.	.	.	.	.	T	T	S			T	T	T	T										T	.	.	.	.	.	.	T
Template Predicted Secondary structure																																																	.	.	.	.	.	.																				.	.	.	.	.	.
Template SS confidence
		143	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180	.	.	.	.	.	.	.	.	.							190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.							.	210
		235	.	.	.	.	240	.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.		.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300	.	.	.	.	.	.	.	.	.	310	.	.	.
Predicted Secondary structure																																																				.
Query SS confidence																																																				.
Query Sequence		P	S	P	L	F	T	D	T	W	T	A	D	I	P	V	G	T	L	C	P	E	W	A	Q	R	L	G	L	P	E	S	V	V	I	S	G	G	A	F	D	C	H	M	G	A	V	G	A	G	A	.	Q	P	N	A	L	V	K	V	I	G	T	S	T	C	D	I	L	I	A	D	K	Q	S	V	G	E	R	A	V
Query Conservation																																																				.
Alig confidence																						.	.	.	.																											.
Template Conservation																						.	.	.	.
Template Sequence		P	S	S	M	L	P	E	V	K	S	N	S	E	V	Y	G	H	T	R	S	.	.	.	.	Y	R	F	Y	G	S	E	V	P	I	A	G	M	A	G	D	Q	Q	A	A	L	F	G	Q	M	A	F	E	K	G	M	I	K	N	T	Y	G	T	G	A	F	I	V	M	N	T	G	E	E	P	L	S	D	N	D	L
Template Known Secondary structure			G	G	G							S									G	.	.	.	.	G	G	G	T	T																			T	T			S	T	T							S	S	S									T						T	T	S
Template Predicted Secondary structure																						.	.	.	.
Template SS confidence
		211	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230					.	.	.	.	.	.	.	.	.	240	.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.
		314	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360	.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.
Predicted Secondary structure
Query SS confidence
Query Sequence		K	G	I	C	G	Q	V	D	G	S	V	V	P	G	F	I	G	L	E	A	G	Q	S	A	F	G	D	I	Y	A	W	F	G	R	V	L	G	W	P	L	E	Q	L	A	A	Q	H	P	E	L	K	T	Q	I	N	A	S	Q	K	Q	L	L	P	A	L	T	E	A	W	A	K	N	P	S	L	D	H	L	P	V
Query Conservation
Alig confidence												.	.																											.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Conservation												.	.																											.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template Sequence		L	T	T	I	G	Y	G	I	N	G	.	.	K	V	Y	Y	A	L	E	G	S	I	F	V	A	G	S	A	I	Q	W	L	R	D	G	L	R	M	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	I	E	T	S	P	Q	S	E	E	L	A	A	K	A	K	G	D	N	E	V	Y
Template Known Secondary structure										T	T	.	.											S		S										T	S			.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	S	S	S	T	T											S		S
Template Predicted Secondary structure												.	.																											.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
Template SS confidence
		288	.	290	.	.	.	.	.	.	.			.	.	300	.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.																						.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.
		394	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420	.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.		.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.
Predicted Secondary structure																																																								.
Query SS confidence																																																								.
Query Sequence		V	L	D	W	F	N	G	R	R	T	P	N	A	N	Q	R	L	K	G	V	I	T	D	L	N	L	A	T	D	A	P	L	L	F	G	G	L	I	A	A	T	A	F	G	A	R	A	I	M	E	C	F	T	D	.	Q	G	I	A	V	N	N	V	M	A	L	G	G	I	A	R	K	N	Q	V	I	M	Q	A	C
Query Conservation																																																								.
Alig confidence																																																								.															.
Template Conservation																																																																							.
Template Sequence		V	V	P	A	F	T	G	L	G	A	P	Y	W	D	S	E	A	R	G	A	V	F	G	L	T	R	G	T	T	K	E	D	F	V	R	A	T	L	Q	A	V	A	Y	Q	S	K	D	V	I	D	T	M	K	K	D	S	G	I	D	I	P	L	L	K	V	D	G	G	A	.	A	K	N	D	L	L	M	Q	F	Q
Template Known Secondary structure						T	T		B		T	T	T		B	T	T										T	T																																								S	G	G	.	G	G
Template Predicted Secondary structure																																																																							.
Template SS confidence
		345	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360	.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.		.	.	.	.	.	.	420	.	.	.
		473	.	.	.	.	.	.	480	.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.	510	.	.	.	.	.	.	.	.	.	520	.	.	.	.	.	.	.	.	.	530	.	.	.	.	.	.	.	.	.	540	.	.	.	.	.	.	.	.	.	550
Predicted Secondary structure
Query SS confidence
Query Sequence		C	D	V	L	N	R	P	L	Q	I	V	A	S	D	Q	C	C	A	L	G	A	A	I	F	A	A	V	A	A	K	V	H	A	D	I	P	S	A	Q	Q	K	M	A	S	A	V	E	K	T	L	Q	P	C	S	E	Q	A	Q	R	F	E	Q	L	Y	R	R	Y	Q	Q	W	A	M	S	A	E	Q	H	Y
Query Conservation
Alig confidence																																								.						.														.
Template Conservation																																								.						.														.
Template Sequence		A	D	I	L	D	I	D	V	Q	R	A	A	N	L	E	T	T	A	L	G	A	A	Y	L	A	G	L	A	V	G	F	W	K	D	L	D	E	L	.	K	S	M	A	E	.	E	G	Q	M	F	T	P	E	M	P	A	E	E	.	R	D	N	L	Y	E	G	W	K	Q	A	V	A	A	T	Q	T	F	K
Template Known Secondary structure						T	S						S	S	S															T	T	S	S							.			T			.									S					.
Template Predicted Secondary structure																																								.						.														.
Template SS confidence
		424	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.		.	.	.	.	.		.	.	.	470	.	.	.	.	.	.	.	.	.		480	.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.